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Citations for Bio Basic :
1 - 50 of 56 citations for 6 METHYL 2 OXO 4 THIOPHEN 2 YL 1 2 3 4 TETRAHYDRO PYRIMIDINE 5 CARBOXYLIC ACID ETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... Microglia were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours.
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bioRxiv - Bioengineering 2023Quote: Macrophages were polarized to M2 with 50 ng/ml interleukin-4 (IL-4, Bio Basic, RC212-15-5) for 48 hours (Ext Fig 1B) ...
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bioRxiv - Biochemistry 2022Quote: The gene encoding SARS-CoV-2 2-170 NSP3 Mac1 was cloned into a pET-11a plasmid (Bio Basic) and transformed into E ...
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bioRxiv - Cell Biology 2023Quote: ... alkaline phosphatase conjugated antibodies were detected colourometrically using BCIP/NBT (nitro-blue tetrazolium and 5- bromo-4-chloro-3’-indolyphosphate) Substrate Solution (#BE116.100ML; Bio Basic Canada Inc). For analysis of bands ...
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bioRxiv - Immunology 2024Quote: ... 1.5 mM MgCl2 and 2 mM MgSO4 (Bio Basic) in a total volume of 20 μL ...
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bioRxiv - Molecular Biology 2023Quote: ... selection was performed using 2 µg/ml of puromycin (Bio Basic) to collect cells expressing the desired shRNAs ...
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bioRxiv - Cancer Biology 2024Quote: ... Citric acid monohydrate (CAS# 5949-29-1) and sodium citrate (CAS# 6132-04-3) from Bio basic Canada Inc ...
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bioRxiv - Neuroscience 2022Quote: ... RT-qPCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Sumo1 (NM_009460.2 ...
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bioRxiv - Molecular Biology 2021Quote: ... supplemented with 1/10 volume of 1.5M Tris-HCl pH 8.8 (Tris Base Fisher BP152-5, Hydrochloric Acid Fisher A144-212) and 1/10 volume 1 M DTT (Bio Basic DB0058). Samples were boiled for 5 minutes before an additional centrifugation at 4 °C at 16,000 x g for 4 minutes.
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bioRxiv - Microbiology 2020Quote: GM (2 mg/mL) solution was prepared using gentamicin sulfate of USP grade produced by BIO BASIC INC (Toronto ...
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bioRxiv - Evolutionary Biology 2023Quote: The wild-type (wt) VIM-2 gene including its signal peptide sequence was synthesized (Bio Basic Inc.) and subcloned into a low-copy number plasmid ...
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bioRxiv - Molecular Biology 2020Quote: ... Forward primer LCHV-MEP93-qF: 5’-GTACTTCATCGCCTACGGAGC-3’ and reverse primer LCHV-MEP93-qR: 5’-TACGTGTGCTTGAGGAGGTC-3’ were synthesized from Bio Basic, Canada ...
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bioRxiv - Neuroscience 2021Quote: ... Real-time quantitative PCR was performed using Green-2-Go qPCR Master Mix (Bio Basic cat# QPCR004-S) with 25 ng cDNA per reaction and primers targeting mouse Gapdh (Forward ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 S-expressing plasmids were codon-optimized and generated by gene synthesis (Bio Basic Asia Pacific) according to GenBank accession QHD43416.1 ...
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bioRxiv - Microbiology 2020Quote: ... the entire genome sequence of SARS-CoV-2 USA/WA1/2020 (GenBank accession no. MN985325) was chemically synthesized (Bio Basic) in five fragments and cloned into pUC57 plasmids containing unique restriction sites ...
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bioRxiv - Biophysics 2024Quote: ... The mutant ΔPAS EAG1 channel in pGH19 with the deletion of the PAS domain residues 2-130 was generated by Bio Basic Inc ...
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bioRxiv - Microbiology 2021Quote: ... a set of overlapping cDNA fragments representing the entire genome of SARS-CoV-2 Wuhan isolate (GenBank: MN908947.3) were chemically synthesized and cloned into pUC57-Kan (Bio Basic Canada Inc). Where appropriate the relevant synthetic cDNA fragment carried the mutation D614G or Y453F + D614G in the viral S gene ...
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bioRxiv - Biophysics 2021Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility ...
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bioRxiv - Biophysics 2022Quote: The gene encoding 419-residue SARS-CoV-2 N protein was purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was cloned into an expression vector pET-28a with a TEV protease cleavage site between N protein and N-terminal 6xHis-SUMO tag used to enhance the solubility (10) ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... They were fixed in 4% paraformaldehyde for 24 h (Bio Basic, Markham, Ontario, Canada), decalcified in 10% EDTA-disodium salt for 6–8 weeks (Kelong ...
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bioRxiv - Genetics 2020Quote: ... and were then fixed using perilymphatic perfusion with 4% paraformaldehyde (PFA) (Bio Basic Inc.,) prepared in phosphate-buffered saline (PBS) ...
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bioRxiv - Genomics 2020Quote: ... and strand-switching oligonucleotides with three O-methyl-guanine RNA bases (PCR_Sw_mod_3G; Bio Basic, Canada). The resulting double-stranded cDNAs were amplified by PCR using KAPA HiFi DNA Polymerase (Kapa Biosystems) ...
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bioRxiv - Biochemistry 2020Quote: The plasmid containing the monomer of a 145 bp of telomeric DNA (5′-ATC-(TTAGGG)23TGAT-3′) flanked by EcoRV and AvaI sites was obtained from Bio Basic Asia Pacific Pte Ltd ...
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bioRxiv - Plant Biology 2023Quote: ... Bicinchoninic acid (BCA) assay (Bio Basic Inc.), or ...
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bioRxiv - Biochemistry 2020Quote: Genomic DNA was isolated using the Bio Basic All-4-One Genomic DNA MiniPrep Kit flowing the manufacturer’s protocol including the RNase treatment (Bio Basic Inc., Markham ON, Canada). The region around the CRISPR PREX1 target site was PCR-amplified using the primer pair DPREX3F (5’-GCACAGAGGGAAAGTCTCGG-3’ ...
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bioRxiv - Bioengineering 2023Quote: ... in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Bioengineering 2023Quote: ... were mixed with ssDNA output oligonucleotides of interest at 2x excess (Table S1) in 1x annealing buffer (5 mM Tris, 1 mM EDTA, 5 mM MgCl, Millipore, cat# 648311-1KG, Bio Basic, cat# SD8135, Bio Basic, cat# MRB0328) and placed into the thermocycler ...
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bioRxiv - Biochemistry 2021Quote: ... and the resultant RNA purified by nucleic acid spin column (Bio Basic). The purity and homogeneity of the RNA was assessed by urea PAGE and A260/A280 ratio (BioDrop μLite ...
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bioRxiv - Cell Biology 2022Quote: ... 3% low-melting point agarose (Bio Basic, catalog # AB0015) was prepared in modified L-15 media and cooled to 37°C ...
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bioRxiv - Genetics 2019Quote: ... containing 5% milk (Bio Basic, cat #NB0669). Membranes were then stained with PTEN antibody (1:1000 ...
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bioRxiv - Cell Biology 2021Quote: ... bacteria cells were spin down and suspended in buffer H (50 mM Tris-HCl pH 8.0, 150 mM NaCl, 10% Glycerol) containing 1 mg/ml lysozyme (Bio Basic, Cat. No. LDB0308-5) and 0.1 % TritonX-100 ...
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bioRxiv - Cancer Biology 2024Quote: Blocking Solution: 3% Albumin Fraction V (Bio Basic; CA#9048-46-8), 0.1% Tween20 (ACP Chemicals ...
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bioRxiv - Microbiology 2023Quote: ... all in the MM medium (carbon sources are purchased from Sigma-Aldrich with the exception of α-ketoglutaric acid purchased from Bio Basic). For the assay with salt ...
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bioRxiv - Biophysics 2021Quote: 6-well cell culture plates (Cell Star, Cat. -No. 657 160) were prepared by melting agarose (purchased from Bio Basic Canada Inc.; AB0015) pouring 3 mL into each well ...
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bioRxiv - Microbiology 2022Quote: ... NaHCO3 or FBS but supplemented with 5 ng/mL IFN-γ (Bio Basic, USA), 400 ng/mL LPS (EMD Millipore ...
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bioRxiv - Neuroscience 2024Quote: ... The membrane was blocked using 5% milk in Tris-Buffered Saline (Bio Basic, Markham, Ontario, Canada) with Tween-20 for 45 minutes at room temperature ...
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bioRxiv - Molecular Biology 2019Quote: ... 5’-GCG GGG ATC AAA TGT CGT TTT G-3’ yielding an amplicon of 135 bp (Fig. S1) were synthesized by Bio Basic Inc ...
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bioRxiv - Immunology 2021Quote: ... and RNA was reverse-transcribed into cDNA using the 5 × All-In-One RT Master Mix (Bio Basic) according to the manufacturer’s specifications (25 °C for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... source 1 agar (BIO BASIC, FB0010), source 2 Agar (Sangon Biotech ...
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bioRxiv - Biophysics 2020Quote: ... the expression vector of the tethered RRM1-RRM2 (5-184) of hnRNPA1 were purchased from a local company (Bio Basic Asia Pacific Pte Ltd), which was subsequently dissected into the isolated RRM1 (5-95 ...
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bioRxiv - Immunology 2020Quote: ... Lm-OVA stocks frozen at −80 C were grown overnight at 37°C in BHI broth supplemented with 5 ug/ml Erythromycin (Bio Basic, Amherst, New York). Then ...
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bioRxiv - Microbiology 2021Quote: ... 50 µg mL−1 of kanamycin (Kan; Bio Basic).
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bioRxiv - Molecular Biology 2022Quote: ... or 1 mM MTX (Bio Basic Inc, Cat# MB0612) was added where relevant and the sample preheated at 37°C for 2 min ...
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bioRxiv - Developmental Biology 2023Quote: ... covered with a 1% agar pad (Agar A, Bio Basic, FB0010 ...
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bioRxiv - Cell Biology 2022Quote: ... Yeast strains were grown in YPD (1% yeast extract (Bio Basic), 2% peptone (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: ... and 140 mM NaCl (SB0476-1; Bio Basic, Markham, Ontario, Canada) in ddH2O] for 1 h ...
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bioRxiv - Molecular Biology 2022Quote: ... 1% sodium dodecyl sulfate (SDS) and 4X Denhardt’s solution (Bio Basic D0062)] ...
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bioRxiv - Microbiology 2023Quote: ... traNAhD4-1 and traNAQU1 were chemically synthesized and obtained from Bio Basic. sfx94 ...
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bioRxiv - Cell Biology 2024Quote: ... and then adding 1mM IPTG (Bio Basic item number 367-93-1) for 4 hours at 37°C with shaking ...
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bioRxiv - Systems Biology 2020Quote: ... Protein production was then induced by the addition of 1 mM IPTG (Bio Basic) and incubation at 16°C overnight with agitation ...