Labshake search
Citations for Echelon Biosciences :
1 - 50 of 63 citations for Protein Kinase A PKA Activity Assay Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2019Quote: ... PI3 kinase activity was measured using PI3 kinase activity ELISA kit from Echelon Biosciences according to the manufacture’s protocol ...
-
bioRxiv - Biochemistry 2023Quote: PI(3,4,5)P3 production was measured by modified PI3-Kinase activity fluorescence polarisation assay (Echelon Biosciences, Salt Lake City, UT, USA). 10μL reactions in 384- well black microtitre plates used 1mM liposomes containing 50μM PI(4,5)P2 ...
-
bioRxiv - Cell Biology 2022Quote: The enzymatic hydrolysis of SM to ceramide and phosphorylcholine by acid sphingomyelinase was measured with the Acid Sphingomyelinase Activity Assay Kit (Echelon Biosciences, K-3200) according to the manufacturer’s instructions ...
-
bioRxiv - Plant Biology 2019Quote: ... a Malachite Green Assay Kit (Echelon Biosciences) was used to detect the liberated phosphate among different PI substrates according to the manufacturer’s protocol ...
-
bioRxiv - Cancer Biology 2020Quote: PI3K activity was determined using a commercially available PI3K ELISA kit (Echelon Biosciences Inc., Salt Lake City, UT) according to the manufacturer's instructions ...
-
bioRxiv - Microbiology 2021Quote: Protein-lipid overlay assays were performed using commercially available PIP strips (Echelon Biosciences Inc.). Nitrocellulose membranes pre-spotted with different phospholipids were blocked with 2% nonfat milk in PBST [PBS and 0.1% Tween-20 (v/v ...
-
bioRxiv - Molecular Biology 2022Quote: ... The PI3KC3 kinase activity assay and the production of phosphatidylinositol (3,4)-bisphosphate [PI(3,4)P2] were measured using a Class III PI3-Kinase Kit according to the manufacturer’s protocols (Echelon Biosciences Inc. Salt Lake City, UT, USA).
-
bioRxiv - Developmental Biology 2022Quote: ... Free phosphate was measured using the Malachite Green assay kit (Echelon Biosciences). Results of three independent experiments were presented as mean ± standard deviation ...
-
bioRxiv - Cell Biology 2022Quote: ... PtdIns(4,5)P2-diC8 (P-4508) and Malachite Green Assay Kit (K-1500) were from Echelon Biosciences (Tebu-Bio), while n-octyl-β-D-glucopyranoside was from Alfa Aesar (J67390.03).
-
bioRxiv - Plant Biology 2019Quote: ... Measurements of PI4P and PI(4,5)P2 levels in the different samples were then performed using a PI(4)P and PI(4,5)P2 Mass ELISA Assay Kit (Echelon Biosciences) by means of competitive ELISA assay ...
-
bioRxiv - Cell Biology 2022Quote: ... the supernatant was retrieved from the beads and its phosphate concentration measured at 620 nm using the Malachite Green Assay Kit (Echelon Biosciences). Beads were then processed for Western blot as above.
-
bioRxiv - Cell Biology 2020Quote: ... Strips were then transferred to blocking buffer containing purified protein of interest: PI(4,5)P2 Grip (1 μg/mL, positive control from Echelon Biosciences), GST (5 μg/mL) ...
-
Nonsense Mediated RNA Decay Is a Unique Vulnerability of Cancer Cells with SF3B1 and U2AF1 MutationsbioRxiv - Cell Biology 2021Quote: ... Activity of the SMG1i against other PI3 kinase family members (PI3Kα, PI3Kβ, PI3Kγ, PI3Kδ, MTOR) was evaluated using Amplified Luminescent Proximity Homogeneous Assay (Alpha) Screen assays (Perkin Elmer and Echelon Biosciences). PI3K or mTOR protein (produced in Sf9 cells ...
-
bioRxiv - Neuroscience 2022Quote: Nitrocellulose-immobilized phospholipids (assay strips Cat. p6002; Echelon Biosciences) were blocked by incubation for 60min with 3% fatty-acid free BSA in TBST (137mM NaCl ...
-
bioRxiv - Neuroscience 2023Quote: ... PIP strip assay (Echelon Biosciences, Salt Lake City, UT) was performed to analyze interactions between purified GFPNaviPA1 from NG108-15 cells and membrane lipids ...
-
bioRxiv - Cell Biology 2020Quote: ... The assay using membrane lipid strips (P-6002, Echelon Biosciences, USA) spotted with 100 pmol of 15 different lipids were used according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: Lipid blot assay was carried out using PIP Strips™ (Echelon Biosciences) following the manufacturer’s instructions ...
-
bioRxiv - Developmental Biology 2020Quote: One-cell stage zebrafish embryos were injected with 10 pg of a cDNA construct containing a fluorescent protein attached to a PIP-binding protein domain [Bodipy-PI (Echelon Biosciences Inc)] ...
-
bioRxiv - Plant Biology 2020Quote: 5μg protein was mixed with 40μl PolyPIPosomes (Echelon Biosciences) in a total volume of 80μl in a final buffer ...
-
bioRxiv - Pathology 2021Quote: Lipid overlay assays were performed using membrane lipid strips (P-6001; Echelon Biosciences) and purified AvrE or MBP proteins ...
-
bioRxiv - Molecular Biology 2023Quote: Lipid binding assay with purified human NLRP3 variants was performed using PIP strips (Echelon Biosciences) according to manufacturer instructions ...
-
bioRxiv - Cell Biology 2021Quote: ... either protein-lipid overlay was used according to the manufacturer’s instructions (Echelon Biosciences) or a vesicle co-sedimentation assay was used as described in (Kooijman et al. ...
-
bioRxiv - Immunology 2021Quote: Lipid binding assay with purified NLRP3 variants was performed using PIP strip (Echelon Biosciences, Cat. no: P-6001) according to manufacturer instructions ...
-
bioRxiv - Plant Biology 2023Quote: Lipid-strip binding assays were performed according to the manufacturer’s instructions (Echelon Biosciences, Salt Lake City, UT, USA). Briefly ...
-
bioRxiv - Plant Biology 2023Quote: ... The His-tagged peptides (GMA4CG_WT and GMA4CG_V6) phospholipid binding assays were performed using the PIP StripsTM (Echelon Biosciences Inc., CA) as previously described (Islam et al. ...
-
bioRxiv - Cell Biology 2023Quote: Lipid binding capacity of recombinant proteins or anti-PS antibody were analyzed using lipid arrays (Echelon Biosciences Inc ...
-
bioRxiv - Microbiology 2020Quote: ... 5 mg parasite protein lysate was precleaned with 600 μL uncoated agarose beads (Echelon Biosciences, Salt Lake City, UT) at 4 °C for 90 min ...
-
bioRxiv - Microbiology 2022Quote: The lipid-peptide overlay assay was performed using FITC-conjugated TB_KKG6K and PIP strips according to the manufacturer’s instructions (Echelon Biosciences, Salt Lake City, UT, USA). To ensure the suitability of the applied detection method ...
-
bioRxiv - Cell Biology 2020Quote: Identification of lipids interacting with recombinant Vps24 protein was performed using PIP-strips (Echelon Biosciences Inc., Salt Lake City, UT, USA) as per manufacturer’s instructions ...
-
bioRxiv - Biochemistry 2023Quote: ... The protein complexes were analysed via silver staining and incubated with lipid coated membranes (PIP-Strips cat. P-6001) (Echelon Bioscience). The PIP strips were subsequently probed with Myc-antibody (1:1000 ...
-
bioRxiv - Cell Biology 2022Quote: ... Wortmannin (1 μM; Echelon biosciences), IPA-3 (1 μM ...
-
bioRxiv - Cell Biology 2022Quote: ... After spotting 1 ng of the purified recombinant proteins on the membrane lipid and PIP strips (P-6002 and P-6001, respectively, Echelon Biosciences, Salt Lake City, UT) as a positive control ...
-
ARL3 Mediates BBSome Ciliary Turnover by Promoting Its Outward Diffusion through the Transition ZonebioRxiv - Cell Biology 2021Quote: ... 20 µl of 1 mM PolyPIPosomesTM (Echelon Biosciences), and 1 ml of binding buffer were mixed ...
-
bioRxiv - Cancer Biology 2022Quote: ... Phosphatidyl-inositol levels were determined with an ELISA detection kit (K-2500S, Echelon Biosciences) and normalized to 100 μg protein.
-
bioRxiv - Microbiology 2021Quote: ... lipids were extracted according to the PI(3)P Mass ELISA Kit (Echelon Biosciences Inc.) protocol ...
-
bioRxiv - Microbiology 2023Quote: ... and PI(3,4,5)P3 content was measured using Echelon’s PIP3 Mass ELISA Kit (Echelon Biosciences), according to the manufacturer’s instructions.
-
bioRxiv - Cancer Biology 2023Quote: ... anti-pNRF2 rabbit monoclonal IgG at 1:2000 dilution and anti-PI4,5P2 mouse monoclonal IgM antibody (#Z-P045, Echelon Biosciences at 1:2000 dilution) was mixed in blocking buffer and incubated with the membrane at 4°C overnight ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse anti-PI(4,5)P2 (1:100; Echelon Biosciences Inc.). Alexa Fluor-conjugated secondary antibodies were used at 1:1000 (Invitrogen) ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse anti-PI(3,4)P2 (1:100; Echelon Biosciences Inc.), mouse anti-PI(4,5)P2 (1:100 ...
-
bioRxiv - Developmental Biology 2020Quote: ... mouse anti-PI(3)P (1:100; Echelon Biosciences Inc.), mouse anti-PI(3,4)P2 (1:100 ...
-
bioRxiv - Developmental Biology 2020Quote: ... 1:10 mouse anti-PI(3)P (Echelon Biosciences, Inc.). Samples were incubated with primary antibodies for 1h at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-PI4P (Z-P004; Echelon Biosciences, PLA at 1/2000), anti-BrdU (Purified Mouse Anti-BrdU Clone 3D4 ...
-
bioRxiv - Cell Biology 2019Quote: PIP2 quantification was done using a competitive ELISA Kit purchased from Echelon Biosciences (Echelon Biosciences, #K-4500) or utilizing a direct ELISA protocol for measuring PIP2 levels as previously modified and described (8) ...
-
bioRxiv - Microbiology 2020Quote: ... 1 μL of 100 μM and 500 μM PI (Echelon Biosciences) and PI(3)P diC 16 (Echelon Biosciences ...
-
bioRxiv - Microbiology 2023Quote: ... A total of 1.0×108 cells were used for PI(3,4,5)P3 quantification using Echelon’s PIP3 Mass ELISA Kit (Echelon Biosciences), according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2022Quote: ... mouse IgM-anti PI4P (Echelon Biosciences Z-P004; 1:100 for immunofluorescence), rabbit-anti TGN46 (Novus Biologicals cat ...
-
bioRxiv - Cell Biology 2022Quote: ... anti-PtdIns-4-P (1:500, Echelon Biosciences cat. no. Z-P004), and anti-insulin (1:1000 ...
-
bioRxiv - Biochemistry 2021Quote: ... and 50 μl of 1 mg/ml di-C16 PtdIns(3)P (Echelon Biosciences), each freshly prepared in chloroform ...
-
bioRxiv - Microbiology 2022Quote: Isolated SCV fraction from infected RAW264.7 macrophages were further processed for lipid isolation and the isolated lipids were quantified using PI(3)P and PI(4)P mass ELISA kits (Echelon Biosciences) as per manufacturer’s protocol.
-
bioRxiv - Cell Biology 2024Quote: ... Mass ELISA kit (# K-4500) and the phosphatidylinositol 3,4,5-trisphosphate (PI(3,4,5)P3) Mass ELISA kit (#K-2500s, Echelon Biosciences Inc, Salt Lake City, UT).