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Citations for Acrobiosystems :
51 - 100 of 105 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: 384 well ELISA plates were coated with 20µl of RBD (SARS-CoV-2 Omicron BA.1, ACROBiosystems) at 1µg/mL in PBS at 4°C overnight ...
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bioRxiv - Immunology 2020Quote: ... His Tag (MALS verified) (Cat. No. S1N-C52H4, ACROBiosystems). Then interact with the first antibody and the second antibody in sequence ...
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bioRxiv - Microbiology 2021Quote: ... A chimeric neutralizing antibody specific against SARS-CoV-2 S protein receptor binding domain (NR-55410) was provided by ACROBiosystems through BEI resources.
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bioRxiv - Cell Biology 2022Quote: The BIAcore 8K system was used to detect the binding of antibodies to the S trimer of SARS-CoV-2 WA1/2020 (AcroBiosystems). The S protein trimer was covalently coupled to the CM5 sensor chip using an amino coupling kit (Cytiva) ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... were coated with recombinant RBD of SARS-CoV-2 wild-type S protein (1 μg/mL, Catalog No. SPD-C82E9, Acrobiosystems) in carbonate buffer (pH 9.6 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... IL-2 (His tag, IL2-H52H8) and IL-2Rα-Fc (ILA-H5251) were from ACROBiosystems (Newark, DE, USA). Mutations in the delta S1 variant (40591-V08H23 ...
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bioRxiv - Microbiology 2022Quote: ... ACE2 competition ELISA was performed by using the SARS-CoV-2 (B.1.1.529) Inhibitor Screening Kit (ACROBiosystems, Cat No. EP-115) according to the recommended protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Immunology 2021Quote: 96-well QuickPlex plates (Meso Scale Discovery, Rockville, MD) were coated with 50 μL of 1 μg/mL SARS-CoV-2 S1 (ACROBiosystems, Newark, DE), diluted in DPBS (Corning ...
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bioRxiv - Genomics 2022Quote: ... and isolated serum was diluted 100-fold using the dilution buffer of a mouse anti-SARS-CoV-2 antibody IgG titer serologic assay kit (ACROBiosystems, RAS-T023). Diluted samples were added to a microplate with pre-coated SARS-CoV-2 Spike protein (2 μg/mL) ...
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bioRxiv - Molecular Biology 2022Quote: The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated alternatively with 4 µg/ml of SARS-CoV-2-S1 (ACROBiosystems, Newark, DE 19711, USA; #S1N-C52H3) or -S2 (ACROBiosystems ...
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bioRxiv - Biochemistry 2020Quote: ACE2-His-Avi (human ACE2 residues 18-740, C-terminal His-Avi tags) (Cat # AC2H82E6) was acquired from ACROBiosystems (Newark, DE). RBD-Fc (SARS-CoV-2 spike protein residues 319-541 ...
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bioRxiv - Immunology 2022Quote: ... or -S2 (ACROBiosystems; #S2N-C52H5) antigen per well (HL-1 ELISA (28)) ...
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bioRxiv - Immunology 2021Quote: ... Levels of anti-spike antibody were measured using a spike trimer ELISA (Acrobiosystems).
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bioRxiv - Immunology 2021Quote: ... or spike proteins (polyclonal anti-spike, Abcam ab272504; anti-RBD, AcroBiosystems SAD-S35-100ug) of SARS-CoV-2 in a total volume of 60ul of VIM were made in a separate 96 well dilution plate to which 60 pfu/well of SARS-CoV-2 (USA-WA1/2020 ...
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bioRxiv - Immunology 2023Quote: ... spike protein (ACROBiosystems, Beijing, China), IL-2 (100 IU/mL) ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of 100 ng/mL of CD200 (Cluster of Differentiation 200/OX-2 membrane glycoprotein) (ACROBiosystems, Catalog No. 50-101-8369) and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif ...
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bioRxiv - Immunology 2021Quote: Anti-spike antibody was depleted from sera using magnetic bead conjugated spike trimer protein (Acrobiosystems). Beads were resuspended in PBS+0.05% BSA ...
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bioRxiv - Bioengineering 2023Quote: ... Fc Tag (ACROBiosystems). To obtain comparable live cell counts between conditions ...
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bioRxiv - Bioengineering 2023Quote: ... Fc Tag (ACROBiosystems).
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bioRxiv - Molecular Biology 2023Quote: ... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used trimeric spike variants protein (ACROBiosystems) such as Delta (B.1.167.2) ...
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bioRxiv - Systems Biology 2022Quote: ... SPIKE Prototype RBD or Omicron RBD (ACROBiosystems, China), nucleic acid and Biotin-Hexapeptide (synthesized by Sangon Biotech ...
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bioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2022Quote: ... Recombinant S protein with S1/S2 cleavage site abolished (ACROBioSystems, China) was used as positive control in western blot.
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bioRxiv - Microbiology 2021Quote: ... His,Avitag™ (SPD-C82E9,Acrobiosystems); SARS-Vov-2 S protein RBD ...
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bioRxiv - Immunology 2023Quote: ... The omicron BA.5 spike trimer (ACROBiosystems SPN-C522e-50ug) at a final concentration of 0.6 mg/mL was mixed with the bispecific antibody 0213 at a molar ratio of 1:1 on ice for 30mins ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged ICAM-1 (IC1-H52H5, ACROBiosystems), B/B homodimeriser (AP20187 ...
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bioRxiv - Immunology 2022Quote: ... (iv) NanoSTING-NS: 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems ...
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bioRxiv - Biochemistry 2020Quote: ... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
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bioRxiv - Immunology 2021Quote: ... His-tagged and Avi-Tagged RBD from ACROBiosystems (Cat. # SPD-C82E9), and His-tagged and Avi-Tagged ACE2 from ACROBiosystems (Cat ...
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bioRxiv - Immunology 2021Quote: ... and His-tagged and Avi-Tagged ACE2 from ACROBiosystems (Cat. # AC2-H82E6). Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher) ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
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bioRxiv - Immunology 2022Quote: ... The antigen panel used in the ELISA includes RBDs of SARS RBD (AcroBiosystems, SPD-S52H6), MERS RBD (AcroBiosystems ...
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bioRxiv - Biophysics 2021Quote: ... The histidine tag was cleaved by human rhinovirus (HRV) 3C protease (AcroBIOSYSTEMS) and further purified by reverse nickel-affinity chromatography ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis, ACROBiosystems EGR-H82E3); biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were coated with recombinant B.1.351 spike (purified and prepared as described in (71)) and RBD (ACROBiosystems, SPD-C52Hp) antigens described in at 2 µg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake, ACROBiosystems EGR-H82E0); biotinylated His-Avi-tagged human TfR (TfR cross-reactivity ...
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Synthetic Biology 2022Quote: ... After incubation ACE2 with a C-terminal His and AviTag (ACE2-Avi, human ACE2 residues 18-740) (ACROBiosystems, Newark, DE) was added to a final concentration of 4 nM and the resulting mixture was incubated at 25°C for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: Recombinant proteins used for surface display flow cytometry and for catalytic uptake of soluble proteins were as follows: biotinylated His-Avi-tagged human PD-L1 ectodomain (ACROBiosystems PD1-H82E5); biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis ...
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bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
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bioRxiv - Immunology 2020Quote: RBD/ACE-2 neutralization ELISA (ACROBiosystems) was performed according to manufacturer instruction ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was obtained from ProSpec and ProL was obtained from Acrobiosystems. Before use ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with recombinant biotinylated ACE2 (ACROBiosystems, AC2-H82E6) and serial dilutions of rCR3022 antibody for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with biotinylated S1 (Acrobiosystems Cat#: S1N-C82E8) or RBD (Acrobiosystems Cat# ...