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Citations for Acrobiosystems :
51 - 100 of 103 citations for Recombinant Human F9 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... The histidine tag was cleaved by human rhinovirus (HRV) 3C protease (AcroBIOSYSTEMS) and further purified by reverse nickel-affinity chromatography ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
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bioRxiv - Immunology 2020Quote: ... Biotin conjugated RBD protein (ACROBiosystems, SPD-C82E9) were coupled with magnetic beads (CELLection Biotin Binder Kit ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: We used trimeric spike variants protein (ACROBiosystems) such as Delta (B.1.167.2) ...
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bioRxiv - Bioengineering 2023Quote: ... such as PE labelled Protein L (ACROBiosystems), or FITC-Labeled Human CD19 (20-291 ...
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bioRxiv - Cell Biology 2024Quote: ... biotinylated human serum albumin b-HSA (70 kg/mol, ACROBiosystems HSA-H82E3, Fisher Scientific), biotinylated heparan sulfate b-HS (synthesized using hydrazone ligation to 12 kg/mol HS and characterized by QCM-D to determine % of biotinylation as described in Thakar et al ...
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bioRxiv - Microbiology 2021Quote: ... Avitag SARS-CoV-2 RBD protein (SPD-C82E9, Acrobiosystems) was diluted to a final concentration of 400 nM in kinectics buffer (1×PBS ...
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bioRxiv - Bioengineering 2020Quote: ... assays were performed in the same manner using human HER2 (100 ng per well, ACROBiosystems) for plate coating ...
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis, ACROBiosystems EGR-H82E3); biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake ...
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bioRxiv - Biophysics 2022Quote: ... Both the WT and Omicron RBD proteins were from ACRObiosystems, Newark ...
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bioRxiv - Immunology 2022Quote: ... Full length SARS-CoV-2 Spike protein (SPN-C52H4; AcroBiosystems) was used as antigen in decreasing amounts starting from 15 to 0.1 μg ...
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bioRxiv - Immunology 2020Quote: SARS-Cov-2 S protein was produced by ACROBiosystems (USA). The sequence contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A ...
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bioRxiv - Immunology 2022Quote: ... and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems, #NUN-C52H8) were mixed with 20µg of NanoSTING ...
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bioRxiv - Immunology 2022Quote: ... we coated 0.5 μg/ml S protein (Acrobiosystems, DE, USA) and 1 μg/ml N protein (Sino Biological ...
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bioRxiv - Bioengineering 2022Quote: ... CAR expression was determined using PE labelled Protein L (ACROBiosystems), or Biotin conjugated CD19 antigen fused recombinant human IgG1 Fc (Miltenyi ...
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bioRxiv - Bioengineering 2023Quote: ... CAR expressions were determined using PE labelled Protein L (ACROBiosystems), or FITC-Labeled Human CD19 (20-291 ...
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bioRxiv - Immunology 2023Quote: ... All SARS-CoV-2 spike proteins were purchased from AcroBiosystems.
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bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake, ACROBiosystems EGR-H82E0); biotinylated His-Avi-tagged human TfR (TfR cross-reactivity ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 S1 protein was purchased from ACROBiosystems (#S1N-C52H3) and used as received ...
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bioRxiv - Biochemistry 2023Quote: ... were coated with 100 ng SARS-CoV-2 spike protein (ACROBiosystems) in 100mM sodium bicarbonate pH 8.8 at 4°C overnight ...
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bioRxiv - Bioengineering 2024Quote: ... Quantification of IL-6 cytokines was conducted using the IL-6 Human ELISA Kit (CRS-B001-96tests, ACROBiosystems). The concentrations were determined by referencing a standard curve ...
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bioRxiv - Immunology 2022Quote: ... (iii) NanoSTING-N: 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems, #NUN-C52H8) was mixed with 20µg of NanoSTING ...
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bioRxiv - Microbiology 2022Quote: SARS-CoV-2 S protein was purchased from ACROBiosystems (Catalog# SPN-C52H9), SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
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bioRxiv - Biochemistry 2022Quote: 200μL 1mg/mL SARS-CoV-2 Spike protein trimer (Acrobiosystems: SPN-C52H9), SARS-CoV-1 Spike trimer (Acrobiosystems ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 super stable trimer spike protein (ACROBiosystems, SPN-C52H9-50UG) was diluted to 100 ng/mL and coated onto 96 well plates using coating buffer (0.1 M Carbonate ...
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bioRxiv - Biochemistry 2023Quote: The SARS-CoV-2 full-length spike protein was purchased from ACROBiosystems. HRP conjugated anti-mouse secondary antibody and horseradish peroxidase substrate were purchased from Thermo Scientific ...
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bioRxiv - Evolutionary Biology 2022Quote: ... A total of 500-700 μL of OD1 yeast cells were labeled with biotinylated human ACE2 (Acrobiosystems #AC2-H2H82E6) at each of the twelve ACE2 concentrations (half-log increments spanning 10 -12.5 – 10 -7 M) ...
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bioRxiv - Immunology 2021Quote: The affinity test of the RBD dimer with human ACE2 by surface plasmon resonance (SPR) was performed by ACROBiosystems. The Fc tagged ACE2 (AC2-H5257 ...
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bioRxiv - Microbiology 2021Quote: ... both the saturating binding concentrations of antibodies and of the ACE2 protein (ACROBiosystems) for the immobilized SARS-CoV-2 spike protein were determined separately ...
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bioRxiv - Microbiology 2021Quote: S protein trimer of SARS-CoV-2 (# SPN-C52H9, Acrobiosystems, 600 μg/mL) and K-874A (11 mg/mL ...
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bioRxiv - Immunology 2022Quote: ... (iv) NanoSTING-NS: 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... EGFR or EGFRvIII proteins (#CD9-H82E9, #EGR-H82E3 and #EGR-H82E0, ACROBiosystems, USA) for 1 h in the dark at 4°C ...
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bioRxiv - Synthetic Biology 2022Quote: ... After incubation ACE2 with a C-terminal His and AviTag (ACE2-Avi, human ACE2 residues 18-740) (ACROBiosystems, Newark, DE) was added to a final concentration of 4 nM and the resulting mixture was incubated at 25°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... or spike proteins (polyclonal anti-spike, Abcam ab272504; anti-RBD, AcroBiosystems SAD-S35-100ug) of SARS-CoV-2 in a total volume of 60ul of VIM were made in a separate 96 well dilution plate to which 60 pfu/well of SARS-CoV-2 (USA-WA1/2020 ...
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bioRxiv - Biochemistry 2020Quote: ACE2-His-Avi (human ACE2 residues 18-740, C-terminal His-Avi tags) (Cat # AC2H82E6) was acquired from ACROBiosystems (Newark, DE). RBD-Fc (SARS-CoV-2 spike protein residues 319-541 ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% human serum albumin [HSA]) and incubated (diluted 100×) with 1 μg/mL C-terminally site-specifically BirA-biotinylated human FcRn (Acrobiosystems, FCM-H82W7) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Biophysics 2020Quote: ... The as-prepared ProG-DNAMeter was then mixed with an equal molar IgG/Fc-fused Human E-cadherin (AcroBiosystems, catalog#: ECD-H5250) at room temperature for 15 min ...
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bioRxiv - Immunology 2021Quote: Anti-spike antibody was depleted from sera using magnetic bead conjugated spike trimer protein (Acrobiosystems). Beads were resuspended in PBS+0.05% BSA ...
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bioRxiv - Microbiology 2021Quote: One ug/ml His-tagged spike RBD protein of SARS-CoV-2 (SPD-C52H3, Acrobiosystems) were immobilized onto 96-well plates (9018 ...
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bioRxiv - Immunology 2023Quote: ... 96-well Nunc MaxiSorp plates were coated with SARS-CoV-2 Wuhan spike protein (AcroBiosystems) at 2µg/ml in carbonate/bicarbonate buffer for 18h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Immunology 2022Quote: ... Indirect ELISAs were performed by coating microtiter plates with 0.5 - 1 μg/mL full length SARS-CoV-2 Spike protein (SPN-C52H4; AcroBiosystems) in 1x PBS overnight at 4 °C (50 μL/ well) ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Molecular Biology 2023Quote: ... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
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bioRxiv - Microbiology 2021Quote: ... A chimeric neutralizing antibody specific against SARS-CoV-2 S protein receptor binding domain (NR-55410) was provided by ACROBiosystems through BEI resources.
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bioRxiv - Immunology 2020Quote: ... while ELISA with monkey sera were performed with a trimeric his-tagged stabilized SARS-CoV-2 Spike protein produced in-house in Expi293 cells or with a commercial RBD (ACROBiosystems). In brief ...
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bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Biochemistry 2023Quote: For serological ELISAs, 30 µL of assorted proteins (SARS-CoV-2 HexaPro S, SARS-CoV-2 XBB.1.5 HexaPro S (AcroBiosystems, SPN-C524i) and SARS-CoV-1 HexaPro S ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibodies were immobilized onto Protein A biosensors (Fortebio) and the threefold serial dilutions of wild-type RBD (ACROBiosystems, Cat No. SPD-C52H3), Alpha RBD (ACROBiosystems ...