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Citations for Acrobiosystems :
51 - 92 of 92 citations for Recombinant Human CTLA4 His tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The binding was detected using streptavidin tagged with HRP (1:5000) (EP-105, Acrobiosystems), and the colour was developed as described above.
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bioRxiv - Cancer Biology 2020Quote: Human CD40 (Acrobiosystems), GITR (Acrobiosystems) ...
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bioRxiv - Microbiology 2023Quote: ... the membrane was incubated with recombinant c-Met (# MET-H5227, ACROBiosystems) followed by an anti human c-Met antibody (# AF276 ...
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bioRxiv - Immunology 2022Quote: ... Recombinant S protein with S1/S2 cleavage site abolished (ACROBioSystems, China) was used as positive control in western blot.
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bioRxiv - Immunology 2020Quote: ... Anti-human CD19 CAR was detected by staining with FITC-conjugated soluble human CD19 (ACROBiosystems) at room temperature for 20 minutes ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant CD70 trimer protein conjugated to Alexa Fluor 647 (Acrobiosystems, cat. # CDL-HA246) was used to evaluate the affinity of truncated CD27-based CARs for their CD70 target ...
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Microbiology 2023Quote: ... For deep mutational scanning ACE2 binding experiments biotinylated dimeric ACE2 was purchased from ACROBiosystems (AC2-H82E7-1mg).
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bioRxiv - Microbiology 2021Quote: ... 12 OD units of yeast were incubated overnight at room temperature with monomeric biotinylated ACE2 (ACROBiosystems AC2-H82E8) across a concentration range of 10−13 M to 10−6 M at 1-log intervals ...
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bioRxiv - Microbiology 2022Quote: ... 12 OD units of yeast were incubated overnight at room temperature with monomeric biotinylated ACE2 (ACROBiosystems AC2-H82E8) across a concentration range of 10−13 M to 10−6 M at 1-log intervals (plus a 0 M ACE2 condition) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... IL-2 (His tag, IL2-H52H8) and IL-2Rα-Fc (ILA-H5251) were from ACROBiosystems (Newark, DE, USA). Mutations in the delta S1 variant (40591-V08H23 ...
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bioRxiv - Bioengineering 2021Quote: ... or FITC-labeled human CD19 from AcroBiosystems following the manufacture’s protocols ...
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bioRxiv - Immunology 2022Quote: ... 3) human ACE2 (ACROBiosystems, Cat# AC2-H82E6); and 4 ...
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bioRxiv - Microbiology 2023Quote: ... 50 ng of human anti-S1 antibody (ACROBiosystems) was mixed with S1 protein and incubated at room temperature for three hours with gentle agitation before adding it to the culture media.
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bioRxiv - Biochemistry 2024Quote: ... human ENPP3 was purchased from AcroBiosystems (#EN3-H52H4). Reactions were performed in buffer containing 20 mM Tris pH 9 ...
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-treated tumor cells were washed with PBS and incubated for 30 min at 4°C with biotinylated SIRPα (bt-SIRPα; #CDA-H82F2, ACROBiosystems, USA), which bound to the unblocked CD47 on BS153 cells ...
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bioRxiv - Biochemistry 2020Quote: ... SARS-CoV-S1 subunit (14-667), SARS-CoV-S (14-1195, R677A) with C-terminal His-tags were purchased from ACROBiosystems. The recombinant SARS-CoV-2-S2 (686-1213) ...
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bioRxiv - Microbiology 2021Quote: The binding kinetics and affinity of recombinant monoclonal antibodies for the SARS-CoV-2 spike protein (ACROBiosystems) were analysed by SPR (Biacore 8K ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl/well of recombinant SARS Cov-2 S1 protein (ACROBiosystems #S1N-C52H3-100ug) at a concentration of 2 μg/ml in PBS and were incubated overnight at 4 °C ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl/well of recombinant SARS Cov-2 S1 protein (ACROBiosystems #S1N-C52H3-100ug) at a concentration of 2 μg/ml in PBS and were incubated overnight at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
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bioRxiv - Synthetic Biology 2020Quote: ... and human Her2-HisTag (AcroBiosystems, Cat. No. HE2-H5225).
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bioRxiv - Synthetic Biology 2020Quote: ... human IgG1 Fc-HisTag (AcroBiosystems, Cat. No. IG1-H5225) and human Her2-HisTag (AcroBiosystems ...
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bioRxiv - Bioengineering 2023Quote: ... culture supernatants were incubated with immobilized human CD47 (ACRObiosystems). Bound SiRPα-Fc was detected with a secondary biotinylated rabbit anti-IgG Fc monoclonal antibody (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Immunology 2024Quote: ... peptide (YPYDVPDYAGAGC) or N-terminally biotinylated HA peptide (Bio-HA) (GL Biochem Ltd., Shanghai, China) or HER2 extracellular domain (ECD) (Acrobiosystems, Newark, DE, USA) was used as antigen in ELISAs ...
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bioRxiv - Immunology 2020Quote: ... or 200ng/mL human Flt3-L (AcroBiosystems, Cat.Nr. FLL-H55H7) as described 20 ...
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bioRxiv - Biochemistry 2022Quote: ... human SIRPα (Glu 31 - Arg 370 (ACROBiosystems, Newark, DE, USA)
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bioRxiv - Bioengineering 2021Quote: ... 50 µL of human ACE2-Fc (ACROBiosystems, Cat#AC2-H5257) at 400 ng/mL was mixed with 50 µL of VHH at 1 µM ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed five times with PBST and incubated for an additional hour with 100 µL/well of 2-fold serially diluted ACE2-Fc (human ACE2 fused to human IgG1 Fc; ACROBiosystems, Newark, DE, Cat#AC2-H5257) in PBSTC (PBS/0.2% casein/0.1% Tween 20) ...
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bioRxiv - Microbiology 2023Quote: ... recombinant HKU1 spike protein (all kind gifts by Dr. Jason Mclellan) SARS-CoV-2 Omicron BA.1 S protein (ACROBiosystems), mouse Fc-tagged SARS-CoV-2 RBD (Sino Biological ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... were coated with recombinant RBD of SARS-CoV-2 wild-type S protein (1 μg/mL, Catalog No. SPD-C82E9, Acrobiosystems) in carbonate buffer (pH 9.6 ...
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bioRxiv - Molecular Biology 2020Quote: ... hACE2-overexpressed Hek293 cells were incubated with 10 uM peptide for 1 hour and then treated and incubated with 5 uM SARS-CoV-1 S1 protein-His Tag (AcroBiosystems, S1N-C52H3-100UG, USA) for 24 hours ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were coated with recombinant B.1.351 spike (purified and prepared as described in (71)) and RBD (ACROBiosystems, SPD-C52Hp) antigens described in at 2 µg/mL ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 ng/well recombinant 2019-nCoV (COVID-19) S protein (Full Length-R683A-R685A-HisTag, ACROBiosystems, Newark, USA) overnight at 4 °C ...
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bioRxiv - Biophysics 2021Quote: ... The histidine tag was cleaved by human rhinovirus (HRV) 3C protease (AcroBIOSYSTEMS) and further purified by reverse nickel-affinity chromatography ...
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bioRxiv - Bioengineering 2020Quote: ... assays were performed in the same manner using human HER2 (100 ng per well, ACROBiosystems) for plate coating ...
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bioRxiv - Bioengineering 2024Quote: ... Quantification of IL-6 cytokines was conducted using the IL-6 Human ELISA Kit (CRS-B001-96tests, ACROBiosystems). The concentrations were determined by referencing a standard curve ...
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bioRxiv - Immunology 2021Quote: The affinity test of the RBD dimer with human ACE2 by surface plasmon resonance (SPR) was performed by ACROBiosystems. The Fc tagged ACE2 (AC2-H5257 ...
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bioRxiv - Bioengineering 2021Quote: ... assays were performed in the same manner using the following proteins and antibodies: human EGFR (100 ng/well, #EGR-H5222 from ACROBiosystems) for plate coating ...
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bioRxiv - Biophysics 2020Quote: ... The as-prepared ProG-DNAMeter was then mixed with an equal molar IgG/Fc-fused Human E-cadherin (AcroBiosystems, catalog#: ECD-H5250) at room temperature for 15 min ...