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Citations for Acrobiosystems :
51 - 76 of 76 citations for RNA Binding Motif Protein 45 RBM45 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ...
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bioRxiv - Immunology 2021Quote: ... or spike proteins (polyclonal anti-spike, Abcam ab272504; anti-RBD, AcroBiosystems SAD-S35-100ug) of SARS-CoV-2 in a total volume of 60ul of VIM were made in a separate 96 well dilution plate to which 60 pfu/well of SARS-CoV-2 (USA-WA1/2020 ...
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bioRxiv - Microbiology 2021Quote: One ug/ml His-tagged spike RBD protein of SARS-CoV-2 (SPD-C52H3, Acrobiosystems) were immobilized onto 96-well plates (9018 ...
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bioRxiv - Immunology 2023Quote: ... 96-well Nunc MaxiSorp plates were coated with SARS-CoV-2 Wuhan spike protein (AcroBiosystems) at 2µg/ml in carbonate/bicarbonate buffer for 18h at 4°C ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl/well of recombinant SARS Cov-2 S1 protein (ACROBiosystems #S1N-C52H3-100ug) at a concentration of 2 μg/ml in PBS and were incubated overnight at 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl/well of recombinant SARS Cov-2 S1 protein (ACROBiosystems #S1N-C52H3-100ug) at a concentration of 2 μg/ml in PBS and were incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Immunology 2023Quote: ... A B.1.617.2 (Delta) SARS-CoV-2 spike antibody (ACROBiosystems; S1N-S58A1) was used to set up a standard curve which was structured using Graphpad prism (Version 9.4.1 ...
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bioRxiv - Immunology 2022Quote: ... Indirect ELISAs were performed by coating microtiter plates with 0.5 - 1 μg/mL full length SARS-CoV-2 Spike protein (SPN-C52H4; AcroBiosystems) in 1x PBS overnight at 4 °C (50 μL/ well) ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Molecular Biology 2023Quote: ... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
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bioRxiv - Immunology 2021Quote: ... Levels of anti-spike antibody were measured using a spike trimer ELISA (Acrobiosystems).
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bioRxiv - Immunology 2020Quote: ... while ELISA with monkey sera were performed with a trimeric his-tagged stabilized SARS-CoV-2 Spike protein produced in-house in Expi293 cells or with a commercial RBD (ACROBiosystems). In brief ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... were coated with recombinant RBD of SARS-CoV-2 wild-type S protein (1 μg/mL, Catalog No. SPD-C82E9, Acrobiosystems) in carbonate buffer (pH 9.6 ...
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bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 ng/well recombinant 2019-nCoV (COVID-19) S protein (Full Length-R683A-R685A-HisTag, ACROBiosystems, Newark, USA) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Biochemistry 2023Quote: For serological ELISAs, 30 µL of assorted proteins (SARS-CoV-2 HexaPro S, SARS-CoV-2 XBB.1.5 HexaPro S (AcroBiosystems, SPN-C524i) and SARS-CoV-1 HexaPro S ...
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bioRxiv - Bioengineering 2024Quote: Recombinant proteins used for surface display flow cytometry and for catalytic uptake of soluble proteins were as follows: biotinylated His-Avi-tagged human PD-L1 ectodomain (ACROBiosystems PD1-H82E5); biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis ...
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bioRxiv - Biochemistry 2020Quote: Recombinant SARS-CoV-2 spike (R683A, R685A, His-tag) protein expressed in HEK 293 cell line was obtained from Acrobiosystems (Newark, DE, USA). Trypsin Gold and Glu-C ...
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bioRxiv - Biophysics 2022Quote: ... 986 and 987 and two alanine substitutions at residues 683 and 685 and a C-terminal T4 fibritin foldon domain to facilitate the protein expression and stabilization of the trimer conformation (ACROBiosystems, Cat No. SPN-C52Hz). All the constructs described above were attached with a C-terminal six-His for protein purification ...
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bioRxiv - Molecular Biology 2020Quote: ... hACE2-overexpressed Hek293 cells were incubated with 10 uM peptide for 1 hour and then treated and incubated with 5 uM SARS-CoV-1 S1 protein-His Tag (AcroBiosystems, S1N-C52H3-100UG, USA) for 24 hours ...
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bioRxiv - Biochemistry 2020Quote: ... fluorescently labeled SARS-CoV-2 spike S1 (10 nM) was mixed with unlabeled antibody (SAD-S35, ACROBiosystems) at increasing concentrations (3.8 pM–250 nM ...
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bioRxiv - Molecular Biology 2022Quote: The IgG ELISAs for RBD were conducted by using Antibody IgG Titer Serologic Assay Kit (AcroBiosystems, RAS-T059). According to the manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and isolated serum was diluted 100-fold using the dilution buffer of a mouse anti-SARS-CoV-2 antibody IgG titer serologic assay kit (ACROBiosystems, RAS-T023). Diluted samples were added to a microplate with pre-coated SARS-CoV-2 Spike protein (2 μg/mL) ...