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Citations for Acrobiosystems :
51 - 79 of 79 citations for 6 Chloro 2 trifluoromethyl imidazo 1 2 b pyridazine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... while ELISA with monkey sera were performed with a trimeric his-tagged stabilized SARS-CoV-2 Spike protein produced in-house in Expi293 cells or with a commercial RBD (ACROBiosystems). In brief ...
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bioRxiv - Cell Biology 2022Quote: The BIAcore 8K system was used to detect the binding of antibodies to the S trimer of SARS-CoV-2 WA1/2020 (AcroBiosystems). The S protein trimer was covalently coupled to the CM5 sensor chip using an amino coupling kit (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were isolated by mechanical disruption of spleen pouch and seeded at a concentration of 5E5 cells/well in a precoated 96-well plate and incubated for 24 h in the presence or absence of 10 µg/ml of SARS-CoV-2 Spike (S1N-C52H4, AcroBiosystems). Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4 ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were isolated by mechanical disruption of spleen pouch and seeded at a concentration of 5E5 cells/well and incubated for 24 h in the presence or absence of 10 μg/ml of SARS-CoV-2 Spike (S1N-C52H4, AcroBiosystems) or Nucleocapsid (NUN-C5227 ...
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bioRxiv - Immunology 2022Quote: ... (iv) NanoSTING-NS: 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems ...
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bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Cell Biology 2024Quote: ... biotinylated human serum albumin b-HSA (70 kg/mol, ACROBiosystems HSA-H82E3, Fisher Scientific), biotinylated heparan sulfate b-HS (synthesized using hydrazone ligation to 12 kg/mol HS and characterized by QCM-D to determine % of biotinylation as described in Thakar et al ...
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bioRxiv - Genomics 2022Quote: ... and isolated serum was diluted 100-fold using the dilution buffer of a mouse anti-SARS-CoV-2 antibody IgG titer serologic assay kit (ACROBiosystems, RAS-T023). Diluted samples were added to a microplate with pre-coated SARS-CoV-2 Spike protein (2 μg/mL) ...
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bioRxiv - Molecular Biology 2022Quote: The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated alternatively with 4 µg/ml of SARS-CoV-2-S1 (ACROBiosystems, Newark, DE 19711, USA; #S1N-C52H3) or -S2 (ACROBiosystems ...
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bioRxiv - Biochemistry 2020Quote: Recombinant SARS-CoV-2 spike (R683A, R685A, His-tag) protein expressed in HEK 293 cell line was obtained from Acrobiosystems (Newark, DE, USA). Trypsin Gold and Glu-C ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of 100 ng/mL of CD200 (Cluster of Differentiation 200/OX-2 membrane glycoprotein) (ACROBiosystems, Catalog No. 50-101-8369) and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed five times with PBST and incubated for an additional hour with 100 µL/well of 2-fold serially diluted ACE2-Fc (human ACE2 fused to human IgG1 Fc; ACROBiosystems, Newark, DE, Cat#AC2-H5257) in PBSTC (PBS/0.2% casein/0.1% Tween 20) ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Microbiology 2021Quote: ... ELISA plates were coated with recombinant B.1.351 spike (purified and prepared as described in (71)) and RBD (ACROBiosystems, SPD-C52Hp) antigens described in at 2 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... BA.1 (ACROBiosystems, SPN-C522a), BA.5 (ACROBiosystems ...
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bioRxiv - Immunology 2023Quote: ... Wuhan or Omicron BA.1 (AcroBiosystems) over a concentration range in carbonate/bicarbonate buffer for 18h at 4°C ...
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bioRxiv - Biophysics 2022Quote: ... His-tagged ICAM-1 (IC1-H52H5, ACROBiosystems), B/B homodimeriser (AP20187 ...
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bioRxiv - Immunology 2022Quote: ... and Omicron BA.1 RBD (ACROBiosystems, SPD-C82E4) was conjugated to streptavidin-AF647 (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: ... SARS-CoV-1 Spike trimer (Acrobiosystems: SPN-S52H5), SARS-CoV-2 Spike D614G (Acrobiosystems ...
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bioRxiv - Immunology 2023Quote: ... or recombinant nucleocapsid protein (Acrobiosystems; 1 µg/ml) was coated on 96-well plate at 4 °C overnight ...
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bioRxiv - Molecular Biology 2020Quote: ... hACE2-overexpressed Hek293 cells were incubated with 10 uM peptide for 1 hour and then treated and incubated with 5 uM SARS-CoV-1 S1 protein-His Tag (AcroBiosystems, S1N-C52H3-100UG, USA) for 24 hours ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Recombinant human ACE2 (Uniprot: Q9BYF1-1) was purchased from ACROBiosystems (AC2-H82E6), consisting of residues 18-740 spanning an intrinsic dimerization domain ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Civet (Paguma larvata) ACE2 (Uniprot: Q56NL1-1) was purchased from ACROBiosystems (AC2-P5248), consisting of residues 18-740 spanning an intrinsic dimerization domain ...
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bioRxiv - Immunology 2020Quote: ... The binding was detected using streptavidin tagged with HRP (1:5000) (EP-105, Acrobiosystems), and the colour was developed as described above.
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Microbiology 2022Quote: ... and 0.5% human serum albumin [HSA]) and incubated (diluted 100×) with 1 μg/mL C-terminally site-specifically BirA-biotinylated human FcRn (Acrobiosystems, FCM-H82W7) in PBS-TH for 30 min at 4 °C with shaking ...
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bioRxiv - Microbiology 2022Quote: ... ELISA plates were coated with recombinant Wuhan-Hu-1 (purified and prepared as described in (61)) or Delta RBD (ACRO Biosystems SPD-C52Hh) and RBD (ACROBiosystems, SPD-C52Hp) antigens at 0.5 µg/mL ...