Labshake search
Citations for Acrobiosystems :
51 - 99 of 99 citations for SARS Coronavirus Spike Glycoprotein S1 His Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with biotinylated S1 (Acrobiosystems Cat#: S1N-C82E8) or RBD (Acrobiosystems Cat# ...
-
bioRxiv - Immunology 2021Quote: SARS-CoV-2 RBD-coated magnetic beads (ACROBiosystems) were prepared according to the manufacturer’s protocol and resuspended in ultrapure water at 1 mg/ml concentration ...
-
bioRxiv - Microbiology 2021Quote: ... 10 nM of SARS-CoV-2 RBD (ACROBiosystems) together with different concentrations of CV38-142 IgG dissolved in the running buffer were injected into the system within 90 s in a flow rate of 30 μl/min followed by a regeneration step between each concentration ...
-
bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 RBD with an AviTag (Acrobiosystems) was immobilized on the streptavidin chip to around 100 RU ...
-
bioRxiv - Microbiology 2021Quote: ... His,Avitag™ (SPD-C82E9,Acrobiosystems); SARS-Vov-2 S protein RBD ...
-
bioRxiv - Biochemistry 2023Quote: For serological ELISAs, 30 µL of assorted proteins (SARS-CoV-2 HexaPro S, SARS-CoV-2 XBB.1.5 HexaPro S (AcroBiosystems, SPN-C524i) and SARS-CoV-1 HexaPro S ...
-
bioRxiv - Immunology 2022Quote: ... Recombinant S protein with S1/S2 cleavage site abolished (ACROBioSystems, China) was used as positive control in western blot.
-
bioRxiv - Immunology 2023Quote: ... The omicron BA.5 spike trimer (ACROBiosystems SPN-C522e-50ug) at a final concentration of 0.6 mg/mL was mixed with the bispecific antibody 0213 at a molar ratio of 1:1 on ice for 30mins ...
-
bioRxiv - Immunology 2020Quote: Assays to determine the binding of the S protein or its RBD of SARS-CoV-2 was performed using the SARS-CoV-2 (COVID-19) Inhibitor Screening Kit from Acrobiosystems (EP-105) as per the manufacture’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Avitag SARS-CoV-2 RBD protein (SPD-C82E9, Acrobiosystems) was diluted to a final concentration of 400 nM in kinectics buffer (1×PBS ...
-
bioRxiv - Biophysics 2022Quote: ... His-tagged ICAM-1 (IC1-H52H5, ACROBiosystems), B/B homodimeriser (AP20187 ...
-
bioRxiv - Immunology 2020Quote: SARS-Cov-2 S protein was produced by ACROBiosystems (USA). The sequence contains AA Val 16 - Pro 1213 (Accession # QHD43416.1 (R683A ...
-
bioRxiv - Immunology 2022Quote: ... (iv) NanoSTING-NS: 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems ...
-
bioRxiv - Biochemistry 2020Quote: ... human ACE2 (Fc-tag) were all purchased from Acrobiosystems (S1N-C52H3 ...
-
bioRxiv - Microbiology 2021Quote: Magnetic beads conjugated to the SARS-CoV-2 RBD (AcroBiosystems, MBS-K002) were prepared according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2021Quote: ... Magnetic beads conjugated to the SARS-CoV-2 RBD (AcroBiosystems, MBS-K002) were prepared according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: SARS-CoV-2 S protein was purchased from ACROBiosystems (Catalog# SPN-C52H9), SARS-CoV-2 E protein was purchased from ThermoFisher Scientific (Catalog# RP-87682) ...
-
bioRxiv - Neuroscience 2023Quote: ... with the addition of 100 ng/mL of CD200 (Cluster of Differentiation 200/OX-2 membrane glycoprotein) (ACROBiosystems, Catalog No. 50-101-8369) and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif ...
-
bioRxiv - Microbiology 2021Quote: S protein trimer of SARS-CoV-2 (# SPN-C52H9, Acrobiosystems, 600 μg/mL) and K-874A (11 mg/mL ...
-
bioRxiv - Immunology 2023Quote: ... or entire S2 domain of SARS-CoV-2 Wuhan-Hu-1 S (ACROBiosystems). Beads were resuspended in PBS + 0.05% BSA ...
-
bioRxiv - Immunology 2021Quote: ... His-tagged and Avi-Tagged RBD from ACROBiosystems (Cat. # SPD-C82E9), and His-tagged and Avi-Tagged ACE2 from ACROBiosystems (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... Magnetic beads conjugated to the SARS-CoV-2 B.1.351 RBD (ACROBiosystems, MBS-K032) were prepared according to the manufacturer’s protocol ...
-
bioRxiv - Immunology 2022Quote: SARS-CoV-2 Wuhan-Hu-1 RBD-coupled magnetic beads (AcroBiosystems Inc, MBS-K002) were reconstituted at 1 mg/ml in assay buffer (PBS + 0.05% BSA ...
-
bioRxiv - Immunology 2021Quote: ... and His-tagged and Avi-Tagged ACE2 from ACROBiosystems (Cat. # AC2-H82E6). Two initial rounds of magnetic-activated cell sorting were performed on the library using decreasing amounts of Ray Biotech RBD-coated His-Tag Dynabeads (Thermo Fisher) ...
-
bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human TfR (TfR cross-reactivity, ACROBiosystems TFR-H82E5); His-tagged mouse TfR (TfR cross-reactivity ...
-
bioRxiv - Immunology 2022Quote: ... The antigen panel used in the ELISA includes RBDs of SARS RBD (AcroBiosystems, SPD-S52H6), MERS RBD (AcroBiosystems ...
-
bioRxiv - Microbiology 2022Quote: Magnetic beads conjugated to the SARS-CoV-2 Wuhan-Hu-1 RBD (ACROBiosystems, MBS-K002) or Delta RBD (ACROBiosystems ...
-
bioRxiv - Biophysics 2021Quote: ... The histidine tag was cleaved by human rhinovirus (HRV) 3C protease (AcroBIOSYSTEMS) and further purified by reverse nickel-affinity chromatography ...
-
bioRxiv - Immunology 2022Quote: ... B cell tetramers were assembled by mixing 100 μg ml−1 of C-terminal AviTagged RBD or S1 (ACROBiosystems) with 100 μg ml−1 of streptavidin-PE (eBiosciences ...
-
bioRxiv - Microbiology 2023Quote: ... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
-
bioRxiv - Microbiology 2021Quote: ... The two libraries were panned separately on biotinylated recombinant SARS-CoV-2 RBD (SPD-C82E9, Acrobiosystems), for 4 rounds with decreasing amount of antigen ...
-
bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
-
bioRxiv - Microbiology 2023Quote: 384 well ELISA plates were coated with 20µl of RBD (SARS-CoV-2 Omicron BA.1, ACROBiosystems) at 1µg/mL in PBS at 4°C overnight ...
-
bioRxiv - Immunology 2021Quote: His-tagged recombinant RBD Beta protein was produced in HEK cells (ACROBiosystems, SPD-C52Hp) and covalently labeled using CruzFluor488 (Santa Cruz Biotechnology ...
-
bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
-
bioRxiv - Biochemistry 2020Quote: ... Data analysis was performed using Byonic 2.3 software and manually using Xcalibur 4.2 and GlycoWorkbench 1.1. The N protein expressed in HEK293 cells (Cat. No. NUN-C5227) was purchased from AcroBiosystems (Newark, DE).
-
bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis, ACROBiosystems EGR-H82E3); biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake ...
-
bioRxiv - Microbiology 2021Quote: ... ELISA plates were coated with recombinant B.1.351 spike (purified and prepared as described in (71)) and RBD (ACROBiosystems, SPD-C52Hp) antigens described in at 2 µg/mL ...
-
bioRxiv - Microbiology 2021Quote: ... A chimeric neutralizing antibody specific against SARS-CoV-2 S protein receptor binding domain (NR-55410) was provided by ACROBiosystems through BEI resources.
-
bioRxiv - Cell Biology 2022Quote: The BIAcore 8K system was used to detect the binding of antibodies to the S trimer of SARS-CoV-2 WA1/2020 (AcroBiosystems). The S protein trimer was covalently coupled to the CM5 sensor chip using an amino coupling kit (Cytiva) ...
-
Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... were coated with recombinant RBD of SARS-CoV-2 wild-type S protein (1 μg/mL, Catalog No. SPD-C82E9, Acrobiosystems) in carbonate buffer (pH 9.6 ...
-
bioRxiv - Bioengineering 2024Quote: ... biotinylated His-Avi-tagged human EGFRvIII ectodomain (EGF variant analysis and soluble EGFR uptake, ACROBiosystems EGR-H82E0); biotinylated His-Avi-tagged human TfR (TfR cross-reactivity ...
-
bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
-
bioRxiv - Microbiology 2022Quote: ... ACE2 competition ELISA was performed by using the SARS-CoV-2 (B.1.1.529) Inhibitor Screening Kit (ACROBiosystems, Cat No. EP-115) according to the recommended protocol ...
-
bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
-
bioRxiv - Genomics 2022Quote: ... and isolated serum was diluted 100-fold using the dilution buffer of a mouse anti-SARS-CoV-2 antibody IgG titer serologic assay kit (ACROBiosystems, RAS-T023). Diluted samples were added to a microplate with pre-coated SARS-CoV-2 Spike protein (2 μg/mL) ...
-
bioRxiv - Molecular Biology 2022Quote: The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
-
bioRxiv - Synthetic Biology 2022Quote: ... After incubation ACE2 with a C-terminal His and AviTag (ACE2-Avi, human ACE2 residues 18-740) (ACROBiosystems, Newark, DE) was added to a final concentration of 4 nM and the resulting mixture was incubated at 25°C for 30 minutes ...
-
bioRxiv - Bioengineering 2024Quote: Recombinant proteins used for surface display flow cytometry and for catalytic uptake of soluble proteins were as follows: biotinylated His-Avi-tagged human PD-L1 ectodomain (ACROBiosystems PD1-H82E5); biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis ...