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Citations for Acrobiosystems :
51 - 71 of 71 citations for Dengue Virus Serotype 4 Envelope Protein HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Immunology 2020Quote: ... were coated with 50 μl/well of recombinant SARS Cov-2 S1 protein (ACROBiosystems #S1N-C52H3-100ug) at a concentration of 2 μg/ml in PBS and were incubated overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... Pre-treated tumor cells were washed with PBS and incubated for 30 min at 4°C with biotinylated SIRPα (bt-SIRPα; #CDA-H82F2, ACROBiosystems, USA), which bound to the unblocked CD47 on BS153 cells ...
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bioRxiv - Immunology 2022Quote: ... Indirect ELISAs were performed by coating microtiter plates with 0.5 - 1 μg/mL full length SARS-CoV-2 Spike protein (SPN-C52H4; AcroBiosystems) in 1x PBS overnight at 4 °C (50 μL/ well) ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Molecular Biology 2023Quote: ... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
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bioRxiv - Bioengineering 2021Quote: ... assays were performed in the same manner using the following proteins and antibodies: human EGFR (100 ng/well, #EGR-H5222 from ACROBiosystems) for plate coating ...
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bioRxiv - Microbiology 2021Quote: ... A chimeric neutralizing antibody specific against SARS-CoV-2 S protein receptor binding domain (NR-55410) was provided by ACROBiosystems through BEI resources.
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bioRxiv - Immunology 2020Quote: ... while ELISA with monkey sera were performed with a trimeric his-tagged stabilized SARS-CoV-2 Spike protein produced in-house in Expi293 cells or with a commercial RBD (ACROBiosystems). In brief ...
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Analysis of spike protein variants evolved in a novel mouse model of persistent SARS-CoV-2 infectionbioRxiv - Microbiology 2023Quote: ... were coated with recombinant RBD of SARS-CoV-2 wild-type S protein (1 μg/mL, Catalog No. SPD-C82E9, Acrobiosystems) in carbonate buffer (pH 9.6 ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated alternatively with 4 µg/ml of SARS-CoV-2-S1 (ACROBiosystems, Newark, DE 19711, USA; #S1N-C52H3) or -S2 (ACROBiosystems ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 ng/well recombinant 2019-nCoV (COVID-19) S protein (Full Length-R683A-R685A-HisTag, ACROBiosystems, Newark, USA) overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... while some plates were coated with 50 ng/ml SARS-CoV-2 S protein RBD or Biotinylated hACE2 (0.2 µg/ml) (EP-105; AcroBiosystems, Newark, USA) overnight at 4°C in sodium carbonate buffer (pH 9.6) ...
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bioRxiv - Biochemistry 2023Quote: For serological ELISAs, 30 µL of assorted proteins (SARS-CoV-2 HexaPro S, SARS-CoV-2 XBB.1.5 HexaPro S (AcroBiosystems, SPN-C524i) and SARS-CoV-1 HexaPro S ...
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bioRxiv - Bioengineering 2024Quote: Recombinant proteins used for surface display flow cytometry and for catalytic uptake of soluble proteins were as follows: biotinylated His-Avi-tagged human PD-L1 ectodomain (ACROBiosystems PD1-H82E5); biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis ...
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bioRxiv - Biochemistry 2020Quote: Recombinant SARS-CoV-2 spike (R683A, R685A, His-tag) protein expressed in HEK 293 cell line was obtained from Acrobiosystems (Newark, DE, USA). Trypsin Gold and Glu-C ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibodies were immobilized onto Protein A biosensors (Fortebio) and the threefold serial dilutions of wild-type RBD (ACROBiosystems, Cat No. SPD-C52H3), Alpha RBD (ACROBiosystems ...
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bioRxiv - Biophysics 2022Quote: ... 986 and 987 and two alanine substitutions at residues 683 and 685 and a C-terminal T4 fibritin foldon domain to facilitate the protein expression and stabilization of the trimer conformation (ACROBiosystems, Cat No. SPN-C52Hz). All the constructs described above were attached with a C-terminal six-His for protein purification ...
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bioRxiv - Molecular Biology 2020Quote: ... hACE2-overexpressed Hek293 cells were incubated with 10 uM peptide for 1 hour and then treated and incubated with 5 uM SARS-CoV-1 S1 protein-His Tag (AcroBiosystems, S1N-C52H3-100UG, USA) for 24 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Polyclonal anti-eIF4A antibody was further purified from 40 ml of serum by running over a 5 ml Protein A column (Acrobiosystems Inc., MA-0422-C5) for immunoprecipitation experiments ...