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Citations for Acrobiosystems :
51 - 100 of 102 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Recombinant S protein with S1/S2 cleavage site abolished (ACROBioSystems, China) was used as positive control in western blot.
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bioRxiv - Evolutionary Biology 2023Quote: ... and incubated with biotinylated monomeric human ACE2 protein (ACROBiosystems AC2-H82E8) across a concentration range from 10−6 to 10−13 M at 1-log intervals ...
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bioRxiv - Immunology 2022Quote: ... (iii) NanoSTING-N: 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems, #NUN-C52H8) was mixed with 20µg of NanoSTING ...
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bioRxiv - Immunology 2022Quote: ... and biotinylated human ACE2 protein (Cat# AC2-H82E6) from AcroBiosystems (Newark, DE). Human C1q protein was a gift from Dr ...
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bioRxiv - Microbiology 2021Quote: ... both the saturating binding concentrations of antibodies and of the ACE2 protein (ACROBiosystems) for the immobilized SARS-CoV-2 spike protein were determined separately ...
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bioRxiv - Microbiology 2022Quote: ... and split into incubations with biotinylated monomeric human ACE2 protein (ACROBiosystems AC2-H82E8) across a concentration range from 10−6 to 10−13 M at 1-log intervals ...
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bioRxiv - Immunology 2022Quote: ... (iv) NanoSTING-NS: 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) and 20µg of nucleocapsid protein-B.1.17 (Acrobiosystems ...
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bioRxiv - Cancer Biology 2023Quote: ... EGFR or EGFRvIII proteins (#CD9-H82E9, #EGR-H82E3 and #EGR-H82E0, ACROBiosystems, USA) for 1 h in the dark at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged recombinant human FcRn (FCGRT-B2M heterodimer) protein was purchased from ACROBiosystems. Bebtelovimab biosimilar and a human IgG1 isotype control antibody were used as controls in the assay ...
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bioRxiv - Cancer Biology 2024Quote: Recombinant CD70 trimer protein conjugated to Alexa Fluor 647 (Acrobiosystems, cat. # CDL-HA246) was used to evaluate the affinity of truncated CD27-based CARs for their CD70 target ...
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bioRxiv - Microbiology 2021Quote: Magnetic beads conjugated to the SARS-CoV-2 RBD (AcroBiosystems, MBS-K002) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: ... Magnetic beads conjugated to the SARS-CoV-2 RBD (AcroBiosystems, MBS-K002) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was obtained from ProSpec and ProL was obtained from Acrobiosystems. Before use ...
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bioRxiv - Immunology 2023Quote: ... A B.1.617.2 (Delta) SARS-CoV-2 spike antibody (ACROBiosystems; S1N-S58A1) was used to set up a standard curve which was structured using Graphpad prism (Version 9.4.1 ...
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bioRxiv - Immunology 2021Quote: ... or spike proteins (polyclonal anti-spike, Abcam ab272504; anti-RBD, AcroBiosystems SAD-S35-100ug) of SARS-CoV-2 in a total volume of 60ul of VIM were made in a separate 96 well dilution plate to which 60 pfu/well of SARS-CoV-2 (USA-WA1/2020 ...
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bioRxiv - Immunology 2023Quote: ... or entire S2 domain of SARS-CoV-2 Wuhan-Hu-1 S (ACROBiosystems). Beads were resuspended in PBS + 0.05% BSA ...
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bioRxiv - Immunology 2021Quote: Anti-spike antibody was depleted from sera using magnetic bead conjugated spike trimer protein (Acrobiosystems). Beads were resuspended in PBS+0.05% BSA ...
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bioRxiv - Microbiology 2021Quote: ... Magnetic beads conjugated to the SARS-CoV-2 B.1.351 RBD (ACROBiosystems, MBS-K032) were prepared according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... SARS-CoV-2 VOC Omicron RBD tagged with his (ACROBiosystems, Cat No. SPD-C522e) was loaded on HIS1K biosensors ...
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bioRxiv - Immunology 2022Quote: SARS-CoV-2 Wuhan-Hu-1 RBD-coupled magnetic beads (AcroBiosystems Inc, MBS-K002) were reconstituted at 1 mg/ml in assay buffer (PBS + 0.05% BSA ...
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bioRxiv - Microbiology 2022Quote: Magnetic beads conjugated to the SARS-CoV-2 Wuhan-Hu-1 RBD (ACROBiosystems, MBS-K002) or Delta RBD (ACROBiosystems ...
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bioRxiv - Molecular Biology 2021Quote: ... 2019-nCoV S protein RBD that was biotinylated through an Avi-tag (AcroBiosystems, Cat nr. SPD-C82E9) was loaded on streptavidin coated Alpha Donor beads (Perkin Elmer ...
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bioRxiv - Microbiology 2024Quote: ... 96-well plates (JET BIOFIL) were coated with recombinant spike proteins of Wuhan-hu-1 (ACROBiosystems, SPN-C52H4), BA.2.75.2 (ACROBiosystems ...
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bioRxiv - Microbiology 2021Quote: ... The two libraries were panned separately on biotinylated recombinant SARS-CoV-2 RBD (SPD-C82E9, Acrobiosystems), for 4 rounds with decreasing amount of antigen ...
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bioRxiv - Immunology 2022Quote: ... (i) NanoSTING-S vaccine was prepared by gently mixing 10µg of trimeric spike protein-B.1.351 (Acrobiosystems, #SPN-C52Hk) with 20µg of NanoSTING ...
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bioRxiv - Molecular Biology 2023Quote: ... produced by Mabylon AG, Schlieren) and on additional his-tagged proteins as controls (his-LAG3 77, his-TIM3 (AcroBiosystems)) was conducted using the same ELISA protocols as outlined above ...
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bioRxiv - Biochemistry 2020Quote: ... fluorescently labeled SARS-CoV-2 spike S1 (10 nM) was mixed with unlabeled antibody (SAD-S35, ACROBiosystems) at increasing concentrations (3.8 pM–250 nM ...
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bioRxiv - Microbiology 2023Quote: 384 well ELISA plates were coated with 20µl of RBD (SARS-CoV-2 Omicron BA.1, ACROBiosystems) at 1µg/mL in PBS at 4°C overnight ...
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bioRxiv - Bioengineering 2021Quote: ... assays were performed in the same manner using the following proteins and antibodies: human EGFR (100 ng/well, #EGR-H5222 from ACROBiosystems) for plate coating ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... IL-2 (His tag, IL2-H52H8) and IL-2Rα-Fc (ILA-H5251) were from ACROBiosystems (Newark, DE, USA). Mutations in the delta S1 variant (40591-V08H23 ...
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bioRxiv - Microbiology 2021Quote: ... were coated with 50 ng/well recombinant 2019-nCoV (COVID-19) S protein (Full Length-R683A-R685A-HisTag, ACROBiosystems, Newark, USA) overnight at 4 °C ...
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bioRxiv - Bioengineering 2024Quote: Recombinant proteins used for surface display flow cytometry and for catalytic uptake of soluble proteins were as follows: biotinylated His-Avi-tagged human PD-L1 ectodomain (ACROBiosystems PD1-H82E5); biotinylated His-Avi-tagged human full-length EGFR ectodomain (VHH nanobody analysis ...
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bioRxiv - Cell Biology 2022Quote: The BIAcore 8K system was used to detect the binding of antibodies to the S trimer of SARS-CoV-2 WA1/2020 (AcroBiosystems). The S protein trimer was covalently coupled to the CM5 sensor chip using an amino coupling kit (Cytiva) ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were isolated by mechanical disruption of spleen pouch and seeded at a concentration of 5E5 cells/well in a precoated 96-well plate and incubated for 24 h in the presence or absence of 10 µg/ml of SARS-CoV-2 Spike (S1N-C52H4, AcroBiosystems). Commercially available ELISPOT plates for evaluation of IL-4 (MuIL4 ...
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bioRxiv - Immunology 2023Quote: ... splenocytes were isolated by mechanical disruption of spleen pouch and seeded at a concentration of 5E5 cells/well and incubated for 24 h in the presence or absence of 10 μg/ml of SARS-CoV-2 Spike (S1N-C52H4, AcroBiosystems) or Nucleocapsid (NUN-C5227 ...
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bioRxiv - Microbiology 2022Quote: ... monoclonal antibodies were immobilized onto Protein A biosensors (Fortebio) and the threefold serial dilutions of wild-type RBD (ACROBiosystems, Cat No. SPD-C52H3), Alpha RBD (ACROBiosystems ...
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bioRxiv - Microbiology 2022Quote: ... ACE2 competition ELISA was performed by using the SARS-CoV-2 (B.1.1.529) Inhibitor Screening Kit (ACROBiosystems, Cat No. EP-115) according to the recommended protocol ...
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bioRxiv - Biophysics 2022Quote: ... 986 and 987 and two alanine substitutions at residues 683 and 685 and a C-terminal T4 fibritin foldon domain to facilitate the protein expression and stabilization of the trimer conformation (ACROBiosystems, Cat No. SPN-C52Hz). All the constructs described above were attached with a C-terminal six-His for protein purification ...
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bioRxiv - Molecular Biology 2020Quote: ... hACE2-overexpressed Hek293 cells were incubated with 10 uM peptide for 1 hour and then treated and incubated with 5 uM SARS-CoV-1 S1 protein-His Tag (AcroBiosystems, S1N-C52H3-100UG, USA) for 24 hours ...
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bioRxiv - Molecular Biology 2022Quote: ... Polyclonal anti-eIF4A antibody was further purified from 40 ml of serum by running over a 5 ml Protein A column (Acrobiosystems Inc., MA-0422-C5) for immunoprecipitation experiments ...
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bioRxiv - Immunology 2021Quote: 96-well QuickPlex plates (Meso Scale Discovery, Rockville, MD) were coated with 50 μL of 1 μg/mL SARS-CoV-2 S1 (ACROBiosystems, Newark, DE), diluted in DPBS (Corning ...
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bioRxiv - Genomics 2022Quote: ... and isolated serum was diluted 100-fold using the dilution buffer of a mouse anti-SARS-CoV-2 antibody IgG titer serologic assay kit (ACROBiosystems, RAS-T023). Diluted samples were added to a microplate with pre-coated SARS-CoV-2 Spike protein (2 μg/mL) ...
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bioRxiv - Molecular Biology 2022Quote: The RBD-binding antibodies against the ancestral strain variant were depleted by using magnetic beads coated with the RBD fragment of ancestral strain SARS-CoV-2 (AcroBiosystems, MBS-K002). According to the manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated alternatively with 4 µg/ml of SARS-CoV-2-S1 (ACROBiosystems, Newark, DE 19711, USA; #S1N-C52H3) or -S2 (ACROBiosystems ...
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bioRxiv - Neuroscience 2023Quote: ... with the addition of 100 ng/mL of CD200 (Cluster of Differentiation 200/OX-2 membrane glycoprotein) (ACROBiosystems, Catalog No. 50-101-8369) and 100 ng/mL of CX3CL1 (Fractalkine/ chemokine (C-X3-C motif ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with recombinant biotinylated ACE2 (ACROBiosystems, AC2-H82E6) and serial dilutions of rCR3022 antibody for 1 h at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated with biotinylated S1 (Acrobiosystems Cat#: S1N-C82E8) or RBD (Acrobiosystems Cat# ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed five times with PBST and incubated for an additional hour with 100 µL/well of 2-fold serially diluted ACE2-Fc (human ACE2 fused to human IgG1 Fc; ACROBiosystems, Newark, DE, Cat#AC2-H5257) in PBSTC (PBS/0.2% casein/0.1% Tween 20) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were stained with either recombinant biotinylated ACE2 ectodomain (ACROBiosystems, AC2-H82E6) or CR3022 antibody (kindly provided by Neil King and Mike Murphy ...
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bioRxiv - Immunology 2022Quote: ... B cell tetramers were assembled by mixing 100 μg ml−1 of C-terminal AviTagged RBD or S1 (ACROBiosystems) with 100 μg ml−1 of streptavidin-PE (eBiosciences ...