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Citations for Sino Biological :
451 - 500 of 513 citations for Vascular Endothelial Cell Growth Factor VEGF Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... cell lysates were thawed on ice and 2.5 U/ml SuperNuclease (Sino Biological Inc, SSNP01) was added to digest DNA for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... cells were treated with varying doses of recombinant human IFN-α2 (Sino Biological; 13833-HNAY), IFN-β (Peprotech ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids in human cells of untagged RFPL4 and PTOV1 were purchased from Sino Biological (products HG25044-UT and #HG20551-UT ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Molecular Biology 2022Quote: ... Bound phages were incubated for 1 hour with 50 μl of M13 HRP-conjugated M13 bacteriophage antibody (Sino Biological Inc; 1:5,000 diluted in PT, 0.05% BSA). The wells were again washed 4 times with PT buffer followed by one wash with PBS ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated with a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2020Quote: ... Free style 293-F cells were grown in SMM 293-TII expression medium (Sino Biological, M293TII) in a shaker incubator at 150 rpm ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then fixed and stained using a rabbit anti-SARS-CoV serum (Sino Biological) and a secondary peroxidase-labelled goat anti-rabbit IgG (Dako) ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated with cross-reactive rabbit anti-SARS-CoV-2 nucleocapsid IgG (Sino Biological) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: Expi293F cells were obtained from ThermoFisher (cat# A14527) and grown in HEK293 Cell Complete Medium (SMM 293-TII, Sino Biological cat# M293TII). For transient overexpression of B55 and PP2Ac constructs ...
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bioRxiv - Microbiology 2020Quote: ... HEK293 cells were transiently transfected by SARS-CoV-2 Spike expression plasmid (Cat: VG40589-UT, Sino Biological) for 24 to 48 hours ...
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bioRxiv - Bioengineering 2021Quote: Expi293 cells were transiently transfected with ACE2 cloned into the pCMV3-C-GFPSpark expression vector (Sino Biological). Cells were incubated at 37°C in 8% CO2 shaking at 350 RPM for 48 hr ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were transiently transfected with a plasmid expressing human DC-SIGN (HG10200-UT; Sino Biological), using Promega FuGENE™ HD Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (Sino Biological) using FuGENE 6 (Promega) ...
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bioRxiv - Immunology 2020Quote: ... Baculovirus-expressed S2 subdomain and HEK293 cell-expressed N protein were obtained from Sino Biological (Chesterbrook, PA) and RayBiotech (Peachtree Corners ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (Sino Biological) using Lipofectamine®3000 (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: Sf21 and High Five insect cells were cultured using SIM-SF and SIM-HF media (Sino Biological) in a nonhumidified shaker at 27 °C ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were cultured in suspension at 27 °C in SIM SF culture medium (Sino Biological, China) and routinely passaged every other day ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...
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bioRxiv - Bioengineering 2020Quote: ... Human-cell-expressed SARS-CoV-2 Spike S1-His recombinant protein was provided by Sino Biological (40591-V08H). The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337) ...
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bioRxiv - Microbiology 2021Quote: ... the proportion of transfected cells was determined by staining with biotinylated RBD (Sino biologicals, Cat: 40592-V08H-B) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... and the number of infected cells was determined using SARS-CoV-S specific mAb (Sino Biological 401430-R001) and fluorescently labeled secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were also stained with SARS-CoV-2 antigens including biotinylated SARS-CoV-2 RBD protein (Sino Biological) and full-length SARS-CoV-2 Spike protein (Sino Biological ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with human recombinant TGF-β1 (5 ng/ml, 10804-HNAC, Sino Biological Inc., Beijing, China), CMLD-2 (LN03203514 ...
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bioRxiv - Microbiology 2023Quote: Cells were then incubated overnight at 4°C with rabbit anti-HCoV-229E nucleocapsid (1:400, Sino Biological) diluted in 1×SEA BLOCK ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates were incubated with 50 μl of Protein A agarose beads (Sino Biological, Chesterbrook, PA, USA) at 4°C for another 2 h ...
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bioRxiv - Microbiology 2022Quote: ... the medium of the transfected cells was replenished with the SMM 293-TII Expression Medium (Sino Biological, M293TII), and the protein-containing supernatant was harvested every three days for 2-3 batches ...
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were cultured in SMM 293-TIS Expression Medium (Serum-free, without L-Glutamine) (Sino Biological). The supernatant ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% formaldehyde in PBS and stained with polyclonal rabbit anti-SARS-CoV Ab (Sino Biological) and secondary Alexa488 conjugated goat-anti-rabbit Ab (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Microbiology 2023Quote: ... the culture medium of the transfected cells was replaced with the SMM 293-TII Expression Medium (Sino Biological, M293TII) 4-6 hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pelleted and resuspended in incubation buffer containing increasing concentrations of recombinant FC-ACE2 (Sino Biological Cat# 10108-H05H-100) followed by incubation for 20 minutes on ice ...
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bioRxiv - Genetics 2020Quote: ... cells were treated with 2 ng/ml of recombinant biotinylated SARS-CoV-2 spike protein RBD (spike protein-RBD, Sino Biological) for 1 hour at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2022Quote: Plasma or memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Immunology 2023Quote: Memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... ID8 and SK-OV-3 cells were stimulated with 2.5 ng/mL murine and human recombinant IL-20 protein (Sino Biological, Beijing, China) for 24 hours ...
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bioRxiv - Immunology 2020Quote: Internalization of CCR7 was also examined with HEK293T cells stably expressing a mouse CCR7-GFP fusion construct (pCMV3-mCCR7-GFPSpark, Sino Biological Inc.). HEK293T cells were transfected with CCR7-GFP with Lipofectamin 2000 (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-1 pseudotyped virus neutralization assay) or 293T cells overexpressing human angiotensin-converting enzyme 2 (293T-hACE2) (Sino Biological Company) (for Pangolin-GD and RaTG13 pseudotyped virus neutralization assay) ...
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bioRxiv - Neuroscience 2019Quote: TSA-201 cells cultured at ~60% confluency were transfected with 1 ug/mL human MUC1 in pCMV3-GFPSpark (Sino Biological, Collegeville, PA; MG50877ACG) using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...