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Citations for Sino Biological :
101 - 150 of 153 citations for Small Airway Epithelial Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the medium of the transfected cells was replenished with the SMM 293-TII Expression Medium (Sino Biological, M293TII), and the protein-containing supernatant was harvested every three days for 2-3 batches ...
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were cultured in SMM 293-TIS Expression Medium (Serum-free, without L-Glutamine) (Sino Biological). The supernatant ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with polyclonal anti-SARS-CoV-2 S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) followed by anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed and incubated in primary conjugated antibodies (EZH2, BD Bioscience; CD133, Miltenyi; CD15, Biolegend; Sox2, Sino Biological Inc. ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% formaldehyde in PBS and stained with polyclonal rabbit anti-SARS-CoV Ab (Sino Biological) and secondary Alexa488 conjugated goat-anti-rabbit Ab (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Microbiology 2023Quote: ... the culture medium of the transfected cells was replaced with the SMM 293-TII Expression Medium (Sino Biological, M293TII) 4-6 hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained in FACS buffer with anti-CEACAM6-FITC antibody (Sino Biological 10823-R408R, Sino Biological, Inc., Wayne, PA) at 10 μg/mL for 30 min at 4°C in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Full-length monoclonal antibodies (mAb) and antibody fragments (Fab) were produced recombinantly in human cells by Sino Biological (Wayne, PA). Fabs were made from mAbs by papain protease digestion ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pelleted and resuspended in incubation buffer containing increasing concentrations of recombinant FC-ACE2 (Sino Biological Cat# 10108-H05H-100) followed by incubation for 20 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Genetics 2020Quote: ... cells were treated with 2 ng/ml of recombinant biotinylated SARS-CoV-2 spike protein RBD (spike protein-RBD, Sino Biological) for 1 hour at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... The cell culture supernatants were collected and analyzed by western blot analysis using a commercial antibody (Sino Biological Inc. Beijing, China) against SARS-CoV-2 RBD.
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bioRxiv - Immunology 2022Quote: Plasma or memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2023Quote: ... Antigen-positive cells were detected by indirect immunofluorescence assay (IFA) using an anti-HCoV-229E nucleoprotein antibody (Sino Biological, Beijing, China).
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Microbiology 2023Quote: ... the SARS-CoV2 infected cells were fixed and immunostained with a SARS-CoV Nucleocapsid Antibody (Sino Biological, Catalogue number: 40143-MM05), followed by HRP-conjugated Goat anti-Mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: Memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Immunology 2021Quote: ... Cells were stained with an anti-SARS antibody (SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit monoclonal antibody; Sino Biological #40143-R001), diluted to 0.125 μg/mL in 3% BSA/PBS blocking solution for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... ID8 and SK-OV-3 cells were stimulated with 2.5 ng/mL murine and human recombinant IL-20 protein (Sino Biological, Beijing, China) for 24 hours ...
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bioRxiv - Immunology 2020Quote: Internalization of CCR7 was also examined with HEK293T cells stably expressing a mouse CCR7-GFP fusion construct (pCMV3-mCCR7-GFPSpark, Sino Biological Inc.). HEK293T cells were transfected with CCR7-GFP with Lipofectamin 2000 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-1 pseudotyped virus neutralization assay) or 293T cells overexpressing human angiotensin-converting enzyme 2 (293T-hACE2) (Sino Biological Company) (for Pangolin-GD and RaTG13 pseudotyped virus neutralization assay) ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Neuroscience 2019Quote: TSA-201 cells cultured at ~60% confluency were transfected with 1 ug/mL human MUC1 in pCMV3-GFPSpark (Sino Biological, Collegeville, PA; MG50877ACG) using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 1:500 dilution ratio of primary antibody specific for SARS-CoV nucleoprotein56 (Rabbit mAb) (Sino Biological Inc. China), which cross-reacting with the NP protein of SARS-CoV–2 as well ...
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bioRxiv - Pathology 2020Quote: ... HEK-Blue SR-AI and HEK-Blue CD36 cells were prepared from the parent cell line (HEK-Blue™ Null1-v) by stable transfection plasmid pCMV/Hygro-SR-AI or pCMV/Hygro-CD36 (Sino Biological Inc.) using Hygromycin as the selective antibiotic ...
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bioRxiv - Molecular Biology 2021Quote: ... Imdevimab/REGN10987 and REGN10989 (Hansen et al., 2020) were cloned in pCMC3-untagged-NCV (SINO Biologics, Cat: CV011) and produced in 293T cells by SINO Biological (Beijing, China). The human IgG1 isotype control antibodies IgG1/κ and IgG1/λ were produced by transfecting FreeStyle 293-F or 293T cells (Fisher Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Calculation of absolute copy number for TH and VGluT2 in each cell was determined with an external calibration curve based on a TH or VGluT2 plasmid cDNA (obtained, respectively, from Sino Biological (Beijing, China) and Harvard University (Boston ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 PSD and pCMV3 containing the SARS-CoV-2/COVID-19 Spike gene were co-transfected into 7 x 105 293F cells using Sinofection (Sino Biological, Beijing, China). The medium was replaced with fresh DMEM containing 10% FBS after overnight incubation ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 3.7% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) and secondary antibody anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Microbiology 2021Quote: 293F cells at the density of 6 × 105 cells/mL were seeded into 100 mL SMM 293-TII serum-free medium (Sino Biological, Cat. No. M293TII) one day before transfection ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...
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bioRxiv - Immunology 2020Quote: ... and 7 days post transfection by gently pipetting cells into medium and labeled with an anti-RBD monoclonal antibody (clone D003 Sino Biological Catalog # 40150-D003) and F(ab’)2-Goat anti-Human IgG-Fc secondary antibody conjugated with R-phycoerythrin (ThermoFisher Catalog # H10104) ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 antigen production in cells was detected by successive incubations with an anti-SARS-CoV nucleoprotein mouse monoclonal antibody (Sino Biological catalog# 40143-MM05), HRP IgG conjugate (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then fixed in 4% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171). Cells were then stained with anti-rabbit-IgG-FITC (Sigma ...
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bioRxiv - Molecular Biology 2022Quote: ... Viral infected cells were revealed by staining using primary monoclonal antibodies directed against SARS CoV-2 nucleocapsid (SARS CoV-2, Sino Biological Inc., 40143-R001) or primary monoclonal mouse antibodies directed against J2 (HCoV-229E ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were plated in 6-well plates (2×105 cells/well) and treated with 1 ng/mL LPS and 20 ng/mL mIFNγ (Sino Biological Inc., Beijing, China) for 24 h ...
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bioRxiv - Cell Biology 2023Quote: Cells were treated with either the recombinant gp120 of HIV-1 Group M Subunit A (Catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H ...