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Citations for Sino Biological :
351 - 394 of 394 citations for Recombinant Rat Sele His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Islets in Akura™ 96 Spheroid Microplate were treated with 0.3 ng/mL or 30 ng/mL of human recombinant IL-1R2 protein (10111-H08H, Sino Biological) for 16 days ...
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bioRxiv - Cell Biology 2023Quote: ... These patterns were then coated overnight at 4°C with a recombinant E-cadherin Fc Tag protein (10204, Sino Biological) at a concentration of 20μg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... and MERS-CoV S2 subdomains as well as recombinant HCoV-NL63 and HCoV-229E S were purchased from Sino Biological.
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bioRxiv - Molecular Biology 2021Quote: ... The rat DDR2/CD167b Gene ORF cDNA clone expression plasmid was obtained from Sino Biologicals (Beijing, China). Pcax Itgb1-FLAG was a gift from Dennis Selkoe and Tracy Young-Pearse (Addgene plasmid 30153 ...
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bioRxiv - Molecular Biology 2020Quote: ... The rat DDR2/CD167b Gene ORF cDNA clone expression plasmid was obtained from Sino Biologicals (Beijing, China) and Native ORF cIAP-2 clone in pCMV vector was purchased from Origene ...
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bioRxiv - Molecular Biology 2022Quote: ... The rat DDR2/CD167b Gene ORF cDNA clone expression plasmid was obtained from Sino Biologicals (Beijing, China). Opti-MEM and Fetal Bovine serum (FBS ...
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bioRxiv - Immunology 2021Quote: ... The protein antigen used for most SARS-CoV-2 ELISAs was recombinant SARS-CoV-2 (2019-nCoV) spike S1-Fc from Sino Biological. The protein antigen used for the SARS-CoV-2 variants included S1 proteins and receptor binding domain (RBD ...
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bioRxiv - Immunology 2022Quote: ... were added to the 96-well plates (100 µL/well; Costar) coated with recombinant SARS-CoV-2 Spike or RBD antigens (Sino Biological) and blocked with 1% BSA for 60 min at 37°C ...
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bioRxiv - Immunology 2022Quote: ... containing 10 μg OVA or 5 μg of the spike protein of SARS-CoV-2 (the receptor-binding domain; recombinant product from Sino Biological) mixed with or without 10 μg or 20 μg S100A4 as indicated ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pelleted and resuspended in incubation buffer containing increasing concentrations of recombinant FC-ACE2 (Sino Biological Cat# 10108-H05H-100) followed by incubation for 20 minutes on ice ...
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bioRxiv - Biochemistry 2020Quote: The recombinant forms of human ACE2 (residues 1-740) and RBD (residues 319-541) were purchased from Sino Biological (Wayne, PA). Both proteins were extensively dialyzed in 150 mM NH4CH3CO2 prior to MS analyses ...
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bioRxiv - Microbiology 2021Quote: ... were washed and coated with 50 μl per well of 2μg/ml of recombinant spike protein (Sino Biological; 40589-V08B1-B), diluted in 0.1% BSA ...
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bioRxiv - Immunology 2020Quote: ... IgGs and recombinant hACE2 (expressed in a bivalent format as a C-terminal IgG1 Fc conjugate; Sino Biological, Cat # 10108-H02H) were tested against the panel of 17 sarbecovirus RBDs ...
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bioRxiv - Genetics 2020Quote: ... cells were treated with 2 ng/ml of recombinant biotinylated SARS-CoV-2 spike protein RBD (spike protein-RBD, Sino Biological) for 1 hour at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated with a rabbit polyclonal antibody directed against recombinant SARS-CoV nucleoprotein (40143-T62, Sino Biological, Beijing, China). A goat anti-rabbit HRP polymer (Envision + ...
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bioRxiv - Immunology 2023Quote: ... and blocking activity of anti-CTLA-4 monoclonal antibodies were determined by ELISA using recombinant human B7.1 (Sino Biological, #10698-HCCH), human CTLA-4 (Sino Biological ...
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with 50 μL of coating buffer containing 100 ng/well recombinant SARS-CoV-2 spike antigens (Sino Biological) at 4 °C overnight ...
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bioRxiv - Microbiology 2020Quote: ... China) were coated with 2 μg/mL (50μL/well) SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) in carbonate bicarbonate buffer pH9.6 and the plates were incubated at 4°C overnight ...
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bioRxiv - Biochemistry 2021Quote: ... or S2-His proteins expressed in HEK293 (S1 and RBD) or insect cells (S2) were used (Sino Biological 40591-V08H, 40592-VNAH, and 40590-V08B). Native mass spectrometry (Olinares et al. ...
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bioRxiv - Biochemistry 2020Quote: ... the “BiEV index (caspase 14)” was used based on the fluorescein-labeled recombinant caspase 14 protein (11856-H07E; Sino Biological, Beijing, China) labeled with NHS-fluorescein (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2022Quote: ... in 1 x TBST (Fisher AAJ77500K2) for 1 hour after the direct addition of 1.5 μl of either recombinant HER4 (Sino Biological 10363-H08H), or recombinant ATF-6 Beta (Abnova H00001388-Q01) ...
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bioRxiv - Biochemistry 2021Quote: ... C5aR1−/− and C5aR2−/− mice on a C57BL/6J genetic background (n=5-15) were administered with recombinant mouse C5a (Sino Biological, China) at a dose of 50 μg kg-1 via intravenous injection (tail vein) ...
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bioRxiv - Bioengineering 2021Quote: ... were coated with 100 mM bicarbonate/carbonate buffer (pH 9.6) containing 0.03 μg/mL of recombinant proteins (VP1) from homologous wild-type viruses (Sino Biological Inc., Beijing, China) overnight at 4 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... ID8 and SK-OV-3 cells were stimulated with 2.5 ng/mL murine and human recombinant IL-20 protein (Sino Biological, Beijing, China) for 24 hours ...
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bioRxiv - Microbiology 2022Quote: ... the PBMCs were cultured for 7 days in presence of 25ng/mL recombinant human macrophage colony-stimulating factor (rh-MCSF, Sino Biological, China). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Immunology 2023Quote: ... each well was transfected with 2 μg eucaryotic recombinant plasmid pCMV3-C-GFPSpark (C-GFPSpark tag, Sino Biological Inc., Cat#VG40608-ACG) encoding the SARS-CoV-2 full-length membrane glycoprotein fused to a green fluorescent protein (GFP) ...
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bioRxiv - Bioengineering 2023Quote: ... The libraries were subjected to five rounds of biopanning against the recombinant SARS-CoV-2 BA.1 RBD protein (Sino Biological Inc.) as described previously38 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were grown till 70-80% confluency and then treated for 48 hours with 1 μM recombinant mouse interferon gamma (Sino Biological, China). Treated cells were detached by trypsinisation and stained with antibodies against the MHC cell surface proteins.
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bioRxiv - Neuroscience 2023Quote: ... and 20 µM ThT in the presence or absence of purified recombinant MDK (1.25 ng/µl equivalent to 47 nM) from Sino Biological (10247-HNAB) or PTN (5 ng/µl equivalent to 327 nM ...
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bioRxiv - Neuroscience 2019Quote: ... CSF and plasma sTREM2 concentrations were calculated using the standard curve generated for each plate using recombinant human TREM2 protein (Sino Biological, Wayne, PA). A dedicated CSF and plasma sample was loaded onto all plates and used to normalize values ...
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bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were firstly pre-treated with the C5aR1 antagonist PMX53 (10 μM) for 20min (37 ℃, 5 % CO2) before stimulated with recombinant human C5a (Sino Biological, Beijing, China) or P32 (100 μM ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated beads were incubated a second time with 2 µg of a recombinant human DC-SIGN protein with a human Fc tag (Sino Biological, Köln, Germany) for 1 h at 4°C with agitation ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng Firefly-luciferase (FLuc) reporter constructs were mixed with storage buffer or storage buffer supplemented with recombinant 4E-BP1 (Sino Biological, 10022-H07E), eIF4A1wt or eIF4A1E183Q (eIF4A1DQAD ...
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bioRxiv - Developmental Biology 2021Quote: ... ELISA plates were coated with a mixture of recombinant human ACVR1 antigens consisting of ACVR1 ECD fused to human G1 Fc (Sino Biologicals,10227-H03B), human ACVR1 ECD fused to human G1 Fc (R&D Systems ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) VWF-bound gcEVs (VWF+gcEVs) obtained by incubation of gcEVs with 10 µg of recombinant (r) human VWF (Sino Biological, Beijing, China); (3 ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Immunology 2021Quote: 96-well microtiter plates were coated over night at 4°C with 100ng per well of recombinant NTD (Sino Biological; Beijing, China #40591-V49H), SARS-CoV-2 Spike S2 or S1 Glycoprotein (Virion\Serion ...
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bioRxiv - Molecular Biology 2020Quote: ... 1A10 were prepared by immunizing BALB/c mice with recombinant SARS-CoV-2 RBD fused with a C-terminal mouse IgGFc tag (Sino Biological Inc, Beijing, China) using previously described protocols (Qu et al. ...
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Cell Biology 2023Quote: Cells were treated with either the recombinant gp120 of HIV-1 Group M Subunit A (Catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H ...
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Biochemistry 2021Quote: ... different concentrations of rat serum (immunized with COVID-eVax vaccine) or anti-SARS-CoV-2 Spike S1 Subunit antibody (Sino Biological, 40150-T62) were added to each well and incubated overnight at 4°C in PBS – 0.05% Tween20 - 1% BSA ...