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Citations for Sino Biological :
501 - 550 of 594 citations for Recombinant PK50 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Plate was coated with SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, His Tag; Sino Biological, Cat# 40591-V08H) at 5 μg/ml at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 1:1000 mouse antibody specific for SARS-CoV-2 nucleocapsid protein (Sino Biological, Beijing, China 40143-MM05) overnight at 4°C in blocking buffer consisting of 2% FBS and 2% BSA ...
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bioRxiv - Microbiology 2020Quote: ... The 96-well plates coated with 0.1μg Spike protein of SARS-CoV-2 from Sino Biological (Product code: 40591-V08H) at 4°C overnight were blocked by 2% BSA/PBST at room temperature for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... Monoclonal antibody (Ab) against SARS-CoV-2 spike protein (IgG1 clone#43, Cat # 40591-MM43) was purchased from Sino Biological, Inc (Wanye ...
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bioRxiv - Immunology 2020Quote: ... Primary antibodies used were against spike protein (S) (Mouse monoclonal, Gene tex (1A9)) or nucleoprotein (N) (Rabbit monoclonal, Sino Biological). Following 3x PBS washes ...
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bioRxiv - Immunology 2021Quote: ... using a rabbit polyclonal antibody specific to SARS-CoV-2 RBD protein (cat. n° 40592-T62, Sino Biological, Beijing, China) (2/5000 ...
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bioRxiv - Biochemistry 2021Quote: Vero E6 cells were incubated with RBD protein (0.45 μg/mL, final concentration) followed by incubation with human anti-RBD antibody (primary antibody) (40150-D003, Sino Biological) and goat anti-human IgG AF488-conjugated antibody (secondary antibody ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates were coated with Spike 1 protein of SARS-CoV-2 (0.1μg/100 μL, Sino Biological, 40591-V08H), the tested sera were diluted at 1:100 and added to each well ...
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bioRxiv - Immunology 2022Quote: ... were coated with SARS-CoV-2 spike protein (SARS-CoV-2 S1+S2_ECD, 200 ng/well) (Sino Biological, Beijing, China) in PBS and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Neuroscience 2022Quote: ... DRG tissue lysates were immunoprecipitated over 2 hours at 4°C with protein A/G coupled with antibodies (rabbit anti-FcγRI 1:1000, Cat: 80016-R015, Sino Biological Inc. ...
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bioRxiv - Synthetic Biology 2022Quote: ... were used to immobilize the peptide hormone and either Protein A (Fc tagged GLP-1 receptor, Sino Biological, 13944-H02H) or Ni-NTA (6x His tagged GH ...
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bioRxiv - Synthetic Biology 2022Quote: ... After bilayer formation beads were washed two more times with PBS and 5pmol of His-tagged proteins added (Sino Biological: ACE2-His 10108-H08H ...
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bioRxiv - Neuroscience 2023Quote: ... a rabbit polyclonal antibody against the SARS-CoV-2 nucleocapsid protein (dilution: 1:4,000, catalog number: 40143-T62, Sino Biological) was applied to the slides for 15 minutes ...
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bioRxiv - Immunology 2022Quote: ... at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological, 40592-V05H) at 4 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... were prepared for H&E staining and immunofluorescence staining for SARS-CoV-2 detection using the Coronavirus nucleocapsid protein (NP) antibody (cat. 40143-MM05, Sino Biological). Images were collected under a Pannoramic MIDI system (3DHISTECH ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 wild-type (WT) and mutated (Beta: K417N-E484K-N501Y, Delta: L452R-T478K, Lambda: L452Q-F490S) RBD proteins (Sino Biological) were separately coated into 96-well plates at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The L19-mTNF fusion protein was expressed by transient gene expression in CHO-S cells and purified from the cell culture supernatant to homogeneity by protein A (Sino Biological) chromatography ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with either 2 ug/ml SARS-CoV-2 Spike Protein ECD (Sino Biological, 40589-V08B1) or 1 ug/ml SARS-CoV-2 Spike-RBD (Sino Biological ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion protein was produced in CHO-S mammalian cells and purified from the cell culture medium by affinity chromatography using a Protein A (Sino Biological) affinity column ...
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bioRxiv - Microbiology 2021Quote: ... of 20 patients with COVID-19 obtained in February and October 2020 in Chongqing were previously reported.2 Eight RBD-specific mAbs with neutralizing capability against SARS-CoV-2 were obtained from the blood samples of COVID-19 convalescent patients.4 DNA sequences encoding reference strain (wild-type, WT) and mutant Spike proteins of SARS-CoV-2 were codon-optimized and synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding reference strain (GenBank: QHD43416) SARS-CoV-2 Spike protein with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: The codon-optimized gene encoding SARS-CoV-2 S protein (GenBank: QHD43416) with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... The rabbit polyclonal antibody against SARS-CoV-2 SP (Cat# 40150-R007) and ACE2 protein (Cat# 40592-T62) were obtained from Sino Biological and anti-HIVp24 monoclonal antibody was described previously [35,36] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The cells were formalin-fixed and ethanol permeabilized followed by incubation with a murine monoclonal antibody which targets the viral nucleocapsid protein (Sino Biological), followed by a secondary anti-mouse IgG peroxidase conjugate (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... RBD protein expression was analyzed using a rabbit polyclonal antibody SARS-CoV-2 Spike RBD Antibody (HRP) (Sino Biological, #40592-T62), which cross-reacts with SARS-CoV RBD protein ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant SARS-CoV-2 proteins S1-614D and S1-614G with polyhistidine-tag (Cat: 40591-V08H, 40591-V08H3) were purchased from Sino Biological. All proteins were quantitated with Qubit Protein Assay (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... specific S protein levels were identified using specific primary antibodies (NB100-56578, Novus Biologicals and 40150-T62-CoV2, Sino Biological Inc.), and HRP conjugated secondary antibody (Protein Simple) ...
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bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
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bioRxiv - Immunology 2021Quote: ... Presence of virus replication in each well was assessed by visual examination of cytopathic effect along with antibody staining for N protein antigen (mouse monoclonal antibody 40143-MM08, Sino Biological) with fluorescent secondary antibody detection ...
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bioRxiv - Microbiology 2021Quote: ... Cells were treated with methanol 0.6% H2O2 and stained for 1 h with a 1:3000 dilution of 40143-R019 rabbit mAb to SARS-CoV-2 nucleocapsid protein (Sino Biological). A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Immunology 2020Quote: The following proteins were coated on 96 well ELISA plates at the following concentrations: SARS-CoV RBD (Sino Biological, 40150-V08B1) at 1 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Western Blot detection of SARS-CoV-2 Spike protein was performed using a polyclonal Spike antibody (Sino Biological Cat. # 40589-V08B1).
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bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... and huCD4 (Met 1-Trp 390; catalog no. 10400-H08H) proteins with C-terminus 6x-His-tags were purchased from Sino Biological.
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding SARS-CoV-2 spike (S) protein (GenBank: QHD43416) with 19 amino acids deletion at the C-terminal was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: 250 μg the extracellular domain of SARS-CoV-2 spike protein fused with His tag (S1+S2 ECD, S, cat.# 40589-V08B1, Sino Biological) was emulsified with 250 μl Freund’s complete adjuvant (F5881-10ML ...
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bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Biochemistry 2022Quote: ... Codon-optimized expression vactors for HA-tagged and GFP-tagged nucleocapsid (N) protein of SARS-CoV-2 virus were purchased from Sino Biologicals US Inc ...
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bioRxiv - Microbiology 2022Quote: ... The vectors expressing the SARS-CoV-2 M and S proteins (with C-terminal HA-tag) were obtained from Sino Biologicals. The pcDNA3.1 (+)-N-eGFP-N plasmid ...