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Citations for Sino Biological :
101 - 150 of 152 citations for Rcombinant Cynomolgus IL2RA Fc tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2022Quote: Nanobody-Fc format were produced through CRO company using HEK293 transient expression system (Sino Biological). Spike protein was expressed and purified using the method published previously (22) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... were coated with 2 µg/ml SARS-CoV-2 Spike RBD-Fc protein (Sino Biological) in coating buffer (0.1 M carbonate-bicarbonate pH 9.4) ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed with PBS-T and further incubated with human ACE2-Fc (Sino Biological) for 2 hours at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... Recombinant His-tagged versions of AGO1 and AGO2 proteins were purchased from Sino Biological (#11225-H07B #11079-H07B, respectively).
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bioRxiv - Microbiology 2022Quote: ... The His-tagged RBD (RBD-His) protein of SARS-CoV-2 was purchased from Sino Biological (Cat: 40592-V08B). The Fc-tagged wild-type and mutant RBDs mentioned were generated as described previously23 ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminally myc-tagged human NOLC1 cDNA (GenBank ID NM_001284388.2) expression plasmid was procured from Sino Biologicals (HG16317-NM). cDNA encoding human IP6K1 domains ...
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bioRxiv - Biophysics 2021Quote: ACE2-Fc (Acro Biosystems, Newark, DE, AC2-H5257) and RBD (Sino Biological, Chesterbrook, PA, 40592-V08B) deglycosylation was performed using Remove-iT PNGase F (Bio-Rad ...
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bioRxiv - Immunology 2020Quote: ... 96 well plate was coated with RBD recombinant protein (0.5 μg/ml, Fc tag; Sino Biological) at 4 oC overnight ...
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bioRxiv - Immunology 2022Quote: ... were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological) for 1 hour at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... His-tagged SARS-CoV-2 Spike protein (5 μl) or His-tagged RBD protein (5 μl at 1.75 μg/μl, purchased from Sino Biological) were first diluted in 45 μl of MBP binding buffer ...
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bioRxiv - Microbiology 2020Quote: ... The expression plasmid for N-terminally myc-tagged hACE2 (pCMV3-SP-Myc-ACE2) was purchased from Sino Biological (HG10108-NM).
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bioRxiv - Cancer Biology 2023Quote: ... followed by applying 100 ng/well (in 100 μL/well volume) of Flag-tagged human CD3δ/ε heterodimer (Sino Biological) in 100 μL of Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Synthetic Biology 2022Quote: ... After bilayer formation beads were washed two more times with PBS and 5pmol of His-tagged proteins added (Sino Biological: ACE2-His 10108-H08H ...
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bioRxiv - Microbiology 2020Quote: ... was collected for 120 s prior to immersing the sensors in a 1:3 serial dilution of his-tagged human ACE2 (Sino Biologicals) ranging from 1,000 to 0.83nm in 10X Kinetics Buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Myc epitope-tagged SARS-CoV-2 (2019-nCoV) spike S cDNA (VG40589-CM) and Flag epitope-tagged human ACE2 cDNA (HG10108-NF) plasmids were purchased from Sino Biological.
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bioRxiv - Microbiology 2021Quote: ... were coated overnight with 1 ug/ml SARS-CoV-2 (2019-nCoV) Spike Receptor Binding Domain (polyhistidine-tagged) recombinant protein (Sino Biological) diluted in PBS ...
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bioRxiv - Microbiology 2022Quote: ... and the C-terminal c-Myc-tagged pCMV3-2019-nCoV-NP-Myc (SARS-2 N) vectors were obtained from Sino Biological. The SARS-CoV-2 variants C-terminal Flag-tagged pReceiver-M39 U.K ...
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bioRxiv - Microbiology 2020Quote: ... and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological). Supernatant containing lentiviral particles was used to transduce more 293T cells in presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2021Quote: ... and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological). Supernatant containing lentiviral particles was used to transduce HOS and CEM.NKr CCR5+ cells in presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV-2 RBD was immobilized on HIS1K biosensors to study the binding kinetics of mFc-tagged hACE2 (10108-H05H; Sino Biological) before being dipped into tubes containing the 1X Kinetics buffer ...
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bioRxiv - Neuroscience 2024Quote: ... The capsids were immobilized on CM5 sensor chip (Cytiva, 29149603) by amine coupling and His-tagged hALPL (Sino Biological, 10440-H08H) was injected at 15.6 -250 nM concentration to measure binding kinetics ...
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bioRxiv - Microbiology 2021Quote: ... the 4-fold serially diluted SARS-CoV-2 spike RBD-Fc recombinant protein (Sino biological, #40592-V02H) and SARS-CoV-2 spike S1 NTD-Fc (Sino biological ...
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bioRxiv - Genetics 2020Quote: ... and incubated overnight in 500 µL of PBS/0.2% BSA containing 150 nM His6-tagged SARS-CoV-2 spike RBD (Sino Biological, Chesterbrook, PA) and 5 µg/mL anti-myc IgY (Aves Labs ...
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bioRxiv - Immunology 2023Quote: ... 26.4 pmol His-Tagged Biotinylated SARS-CoV-2 (2019-nCoV) spike RBD Recombinant Protein (Sino Biological Inc.; cat number: 40592-VO8H-B) was firstly incubated for 1 hour with 3.78 pmol Streptavidin Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant human ACE2 linked to the Fc domain of human IgG1 (ACE2-Ig) was purchased from Sino Biological Inc ...
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bioRxiv - Microbiology 2021Quote: ... MERS and SARS CoV-1 S1-Fc fusion proteins were prepared by Sino Biological (Vendor #2; Beijing, China) under request ...
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bioRxiv - Microbiology 2021Quote: ... S1 domain of SARS-CoV-2 spike C-terminally fused with Fc (Sino Biological #40591-V02H, 1μg/ml) at 4 °C for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... S1 domain of SARS-CoV-2 spike C-terminally fused with Fc (Sino Biological #40591-V02H, 1μg/ml) at 4°C for 30 min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Recombinant SARS-CoV-2 RBD fused C-terminally to rabbit IgG Fc (RBD::rFc, # 40592-V31H Sino Biological) was used as a specific competitor ...
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bioRxiv - Microbiology 2021Quote: ... the library were incubated with SARS-CoV-2 RBD containing a mouse Fc tag (Sino Biological, 40592-V05H), and biotinylated hACE2 (Kactus ...
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bioRxiv - Microbiology 2020Quote: ... S1 domain of SARS-CoV-2 spike C-terminally fused with Fc (Sino Biological #40591-V02H, 1μg/ml), or the anti-ACE2 antibody (Sino Biological #10108-RP01 ...
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bioRxiv - Cell Biology 2023Quote: A plasmid for Dox-inducible expression of C-terminal HA epitope-tagged RACK1 was generated using a cDNA for RACK1 that was amplified from pCMV3-RACK1 (Sino Biologicals, HG16196-CY) and subcloned into the pInducer20 vector (Addgene ...
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bioRxiv - Biophysics 2020Quote: ... the substrate was incubated with 0.1 mg/ml recombinant E-cadherin-Fc for 2 hours (Sino Biological, Beijing, China). Afterwards ...
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bioRxiv - Immunology 2021Quote: ... One hundred μL of 20 μg/mL 2019-nCoV Spike Protein S1 (Fc Tag) (Sino Biological, CN: 40591-V02H) was mixed with 100 μL of antisera dilutions (serially 5-fold diluted from 1:5 to 1:3,125 ...
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bioRxiv - Biophysics 2023Quote: ... were coated with protein A (Prospec, East Brunswick, NJ) and then with E-cadherin-Fc (Sino Biological, Beijing, China). To obtain the first coating with protein A ...
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bioRxiv - Genetics 2020Quote: ... Loaded sensors were dipped into recombinant SARS-Cov-2 His-tagged Spike protein (D614 or D614G, Sino Biological, Cat # 40591-V08H and 40591-V08H3).
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bioRxiv - Microbiology 2020Quote: ... the S1 domain of the SARS-CoV-2 spike C-terminally fused with Fc (Sino Biological #40591-V02H, 1μg/ml), at 4 °C for 30 min ...
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bioRxiv - Immunology 2022Quote: ... The spike expression on cell surface were detected by staining cells with human ACE2–Fc chimera (Sino Biological, 10108-H02HG) in MACS buffer (D-PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Microbiology 2021Quote: ... The loaded sensors were dipped into twofold serial dilutions from 245.8 nM of the SARS-CoV-2 (2019-nCoV) Spike S1-Fc Recombinant Protein (Sino Biological) to measure a 180-sec specific binding at the association step ...
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bioRxiv - Cell Biology 2023Quote: ... These patterns were then coated overnight at 4°C with a recombinant E-cadherin Fc Tag protein (10204, Sino Biological) at a concentration of 20μg/mL ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells were seeded in 60 mm plates then transfected with 2 µg of N-terminal tagged GFP-HMGB1 DNA plasmid DNA (Sino biological, cat no. HG10326-ANG). In brief ...
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bioRxiv - Immunology 2021Quote: ... The protein antigen used for most SARS-CoV-2 ELISAs was recombinant SARS-CoV-2 (2019-nCoV) spike S1-Fc from Sino Biological. The protein antigen used for the SARS-CoV-2 variants included S1 proteins and receptor binding domain (RBD ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pelleted and resuspended in incubation buffer containing increasing concentrations of recombinant FC-ACE2 (Sino Biological Cat# 10108-H05H-100) followed by incubation for 20 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... IgGs and recombinant hACE2 (expressed in a bivalent format as a C-terminal IgG1 Fc conjugate; Sino Biological, Cat # 10108-H02H) were tested against the panel of 17 sarbecovirus RBDs ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 His-tagged Spike (S) protein and the receptor binding domain (RBD) protein of S were purchased from Sino Biological (Beijing/China and Wayne/USA).
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
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bioRxiv - Immunology 2022Quote: ... pre-incubated beads were incubated a second time with 2 µg of a recombinant human DC-SIGN protein with a human Fc tag (Sino Biological, Köln, Germany) for 1 h at 4°C with agitation ...
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bioRxiv - Developmental Biology 2021Quote: ... ELISA plates were coated with a mixture of recombinant human ACVR1 antigens consisting of ACVR1 ECD fused to human G1 Fc (Sino Biologicals,10227-H03B), human ACVR1 ECD fused to human G1 Fc (R&D Systems ...