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Citations for Sino Biological :
301 - 350 of 709 citations for Pops Hrms Hch Clean Up Spike 13C6 99% 50X Stock 1 Ug Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... RBD protein expression was analyzed using a rabbit polyclonal antibody SARS-CoV-2 Spike RBD Antibody (HRP) (Sino Biological, #40592-T62), which cross-reacts with SARS-CoV RBD protein ...
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bioRxiv - Microbiology 2020Quote: ... and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological). Supernatant containing lentiviral particles was used to transduce more 293T cells in presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2021Quote: ... and a lentiviral transfer plasmid coding for a GFP-tagged SARS-CoV-2 Spike (pLV-SARS-CoV-2 S C-GFPSpark tag) (Sino Biological). Supernatant containing lentiviral particles was used to transduce HOS and CEM.NKr CCR5+ cells in presence of 5μg/mL polybrene ...
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bioRxiv - Microbiology 2020Quote: ... Some initial optimization experiments utilized recombinant SARS-CoV-2 RBD and recombinant SARS-CoV-2 spike extracellular domain purchased from Sino Biological and Genscript ...
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bioRxiv - Microbiology 2020Quote: ... plates were emptied and incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:5,000 in PBSK for 2 hours at room temperature ...
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bioRxiv - Immunology 2020Quote: ... HIV-1 CH505TF SOSIP trimer and commercially-obtained constructs of SARS-CoV-2 spike ectodomain (S1+S2 ECD, S2 ECD and RBD) (Sino Biological Inc cat# 40589-V08B1 and 40590-V08B respectively and RBD from Genescript cat# Z03483 ...
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bioRxiv - Immunology 2020Quote: ... Spike S1-His Recombinant Protein (40591-V08B1) and SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein (40590-V08B) were obtained from Sino Biological. SARS-CoV-2 Spike protein (RBD ...
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding SARS-CoV-2 spike (S) protein (GenBank: QHD43416) with 19 amino acids deletion at the C-terminal was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Immunology 2020Quote: ... were coated with 2 μg/ml of recombinant SARS-CoV-2 proteins (S1 and RBD-His proteins were produced in the lab and S1 +S2 ECD spike protein was purchased from Sino Biologicals) in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Microbiology 2021Quote: 250 μg the extracellular domain of SARS-CoV-2 spike protein fused with His tag (S1+S2 ECD, S, cat.# 40589-V08B1, Sino Biological) was emulsified with 250 μl Freund’s complete adjuvant (F5881-10ML ...
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bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... The plate was washed for 3 times then incubated with mouse sera and SARS-CoV-2 Spike antibody (Sino Biological, China) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2022Quote: ... purified SARS-CoV-2 spike-specific monoclonal rabbit primary antibodies R001 and R007 (cat. n° 40592-R001 and cat. n° 40150-R007, Sino Biological)
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bioRxiv - Microbiology 2022Quote: The following antibodies were used for flow cytometry: rabbit monoclonal SARS-CoV-2 spike-specific antibody [R001] (Cat. n° 40592-R001, Sino Biological), mouse monoclonal SARS-CoV-2 spike-specific antibody [MM57] (cat ...
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bioRxiv - Immunology 2023Quote: ... RBD (Cat. No. 40592-VNAH) and Spike S1 B.1.1.7 variant (Cat. no. 40591-V08H12) were acquired from Sino Biological (Beijing, China) and biotinylated using EZ-Link™ Sulfo-NHS-Biotin kit (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with 50 μL of coating buffer containing 100 ng/well recombinant SARS-CoV-2 spike antigens (Sino Biological) at 4 °C overnight ...
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bioRxiv - Immunology 2024Quote: ... were coated with 100 ng/well of SARS-CoV-2 (2019-nCOV) Spike S1 + S2 ECD-His-Recombinant Protein (40589-V08B1, Sino Biological) overnight at 4°C ...
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Resolution of SARS-CoV-2 infection in human lung tissues is driven by extravascular CD163+ monocytesbioRxiv - Immunology 2024Quote: ... The anti-SARS-CoV-2 (2019-nCoV) Spike RBD-mFc clone #57 used as a control for neutralization assays was acquired from Sino Biologicals.
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bioRxiv - Microbiology 2024Quote: ... Sections of the head were prepared to expose different regions of the nasal cavity and were stained with hematoxylin-eosin or an immunohistochemistry (IHC) staining kit using a SARS-CoV-2 spike RBD-specific antibody (Sino Biological). The damage score of each section was defined as 0 ...
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bioRxiv - Immunology 2022Quote: ... NP (0.5 μg/ml) or M1 (0.25 μg/ml) protein of A/Anhui/1/2013 (Sino biologicals). The next day ...
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bioRxiv - Immunology 2022Quote: ... and 1 μg/ml N protein (Sino Biological, PA, USA) onto ELISA plates (Corning ...
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bioRxiv - Biochemistry 2023Quote: ... and 1 µg/ml human Dectin-2-Fc (Sino Biological) in TSMBB followed by 5 µg/ml anti-mouse IgG AF 488 (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The presence of SARS-CoV-2 Spike was confirmed by western blot with anti-SARS-CoV-2 S1 (Sino Biological # 40150-D004) and anti-SARS-CoV-2 S2 (Thermo Fisher # PA5-112048) ...
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bioRxiv - Genetics 2020Quote: ... and incubated overnight in 500 µL of PBS/0.2% BSA containing 150 nM His6-tagged SARS-CoV-2 spike RBD (Sino Biological, Chesterbrook, PA) and 5 µg/mL anti-myc IgY (Aves Labs ...
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bioRxiv - Bioengineering 2020Quote: ... and SARS-CoV-2 (2019-nCoV) and RBD of spike glycoprotein (mFC tag)(40592-V05H) were purchased from Sino Biological (Beijing, China).
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
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bioRxiv - Microbiology 2020Quote: ... The horses were inoculated subcutaneously with SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) antigens containing 1.0mg ...
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bioRxiv - Immunology 2023Quote: ... 26.4 pmol His-Tagged Biotinylated SARS-CoV-2 (2019-nCoV) spike RBD Recombinant Protein (Sino Biological Inc.; cat number: 40592-VO8H-B) was firstly incubated for 1 hour with 3.78 pmol Streptavidin Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... plates were coated with 1 μg ml−1 of SARS-CoV-2 S protein (Sino Biological) or SARS-CoV-2 RBD protein (Aaron Schmidt ...
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bioRxiv - Cancer Biology 2020Quote: ... R-spondin 500□ng□ml−1 (R&D or Sino Biological), N-acetyl-L-cysteine 1□μM (Sigma-Aldrich) ...
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bioRxiv - Immunology 2021Quote: ... R-spondin 500 ng ml−1 (R&D or Sino Biological), N2 1X (Life Technologies) ...
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bioRxiv - Immunology 2021Quote: ... rabbit anti-C1-INH (1 µg/mL, Sino Biologicals, 10995-R018) or rabbit anti-human C1q (1:300) ...
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bioRxiv - Molecular Biology 2021Quote: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with C terminal OFP Spark fluorescent tag (pCMV3-C-OFPSpark) was obtained from Sino biological (#VG40591-ACR). Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
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bioRxiv - Bioengineering 2021Quote: ... was incubated with 5 ng/µl of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein (Sino Biological #40589-V08B1) in phosphate buffer (pH 6.0) ...
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bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 S expression plasmid pC-SARS2-S was created by inserting the Acc65I/NotI-digested PCR-amplified SARS-CoV-2 S fragment of pCMV3-2019-nCoV-Spike(S1+S2)-long (Sino Biological; VG40589-UT) into the corresponding site of pCAGGS ...
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bioRxiv - Synthetic Biology 2022Quote: ... nanobodies were preincubated with 4 nM SARS-CoV-2 spike protein receptor binding domain fused to an Fc tag (RBD-Fc, SARS-CoV-2 spike protein residues 319-541) (Sino Biological, Wayne, PA) in PBS supplemented with 0.05 mg/mL BSA at 25°C for 30 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... different concentrations of rat serum (immunized with COVID-eVax vaccine) or anti-SARS-CoV-2 Spike S1 Subunit antibody (Sino Biological, 40150-T62) were added to each well and incubated overnight at 4°C in PBS – 0.05% Tween20 - 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 PSD and pCMV3 containing the SARS-CoV-2/COVID-19 Spike gene were co-transfected into 7 x 105 293F cells using Sinofection (Sino Biological, Beijing, China). The medium was replaced with fresh DMEM containing 10% FBS after overnight incubation ...
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Immunology 2022Quote: ... SARS-CoV-2 S1 and SF were detected by a rabbit polyclonal anti-SARS-CoV-2 Spike RBD antibody (Sino Biological, 40592-T62). S2 protein was detected by rabbit polyclonal anti-SARS-CoV-2 Spike S2 antibody (Sino Biological ...
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bioRxiv - Microbiology 2022Quote: ... followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher, #26183) or an antibody against SARS-CoV Spike protein (Sino Biologicals, #40590-T62).
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% BSA in TBS + 0.1% Tween-20 (TBST) at room temperature before incubation at 4°C overnight with primary antibodies: SARS-CoV-2 spike RBD (Sino Biological, 40592-T62), GAPDH (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... vaccines were formulated in 100 μL of PBS 1X and contained a 10 µg antigen dose of spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (Sino Biological 40589-V08H4) and a 20 µg 30% CpG-NPs adjuvant dose ...
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bioRxiv - Microbiology 2020Quote: ... DCs were pulsed with 20μg/mL SSP-1 peptide in the presence of 3μg/mL β-microglobulin (Sino Biological) at 37°C for 3 hours before use ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Genetics 2020Quote: ... Loaded sensors were dipped into recombinant SARS-Cov-2 His-tagged Spike protein (D614 or D614G, Sino Biological, Cat # 40591-V08H and 40591-V08H3).
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bioRxiv - Microbiology 2020Quote: A full-length wild-type pCMV3-SARS-CoV-2 Spike (S1+S2)-long (termed as pCMV-S, codon-optimized, Sino Biological, cat # VG40589-UT) plasmid was used as a template to generate tagged pCMV-S ...