Labshake search
Citations for Sino Biological :
651 - 700 of 710 citations for Myelin protein P0 MPZ Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Microbiology 2020Quote: ... Recombinant SARS-CoV-2 proteins S1-614D and S1-614G with polyhistidine-tag (Cat: 40591-V08H, 40591-V08H3) were purchased from Sino Biological. All proteins were quantitated with Qubit Protein Assay (Thermo Fisher Scientific) ...
-
bioRxiv - Microbiology 2020Quote: ... specific S protein levels were identified using specific primary antibodies (NB100-56578, Novus Biologicals and 40150-T62-CoV2, Sino Biological Inc.), and HRP conjugated secondary antibody (Protein Simple) ...
-
bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
-
bioRxiv - Immunology 2021Quote: ... Presence of virus replication in each well was assessed by visual examination of cytopathic effect along with antibody staining for N protein antigen (mouse monoclonal antibody 40143-MM08, Sino Biological) with fluorescent secondary antibody detection ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were treated with methanol 0.6% H2O2 and stained for 1 h with a 1:3000 dilution of 40143-R019 rabbit mAb to SARS-CoV-2 nucleocapsid protein (Sino Biological). A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma ...
-
bioRxiv - Immunology 2020Quote: The following proteins were coated on 96 well ELISA plates at the following concentrations: SARS-CoV RBD (Sino Biological, 40150-V08B1) at 1 µg/ml ...
-
bioRxiv - Cell Biology 2020Quote: Caco-2 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
-
bioRxiv - Microbiology 2021Quote: ... Western Blot detection of SARS-CoV-2 Spike protein was performed using a polyclonal Spike antibody (Sino Biological Cat. # 40589-V08B1).
-
bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding SARS-CoV-2 spike (S) protein (GenBank: QHD43416) with 19 amino acids deletion at the C-terminal was synthesized by Sino Biological Inc (Beijing ...
-
bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
-
bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
-
bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
-
bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
-
bioRxiv - Biochemistry 2022Quote: ... Codon-optimized expression vactors for HA-tagged and GFP-tagged nucleocapsid (N) protein of SARS-CoV-2 virus were purchased from Sino Biologicals US Inc ...
-
bioRxiv - Microbiology 2022Quote: ... The vectors expressing the SARS-CoV-2 M and S proteins (with C-terminal HA-tag) were obtained from Sino Biologicals. The pcDNA3.1 (+)-N-eGFP-N plasmid ...
-
bioRxiv - Microbiology 2022Quote: ... The copies of the phage-packaged NP protein were quantified by Western blotting using the commercial rabbit anti-NP antibody (Sino Biological) and NP protein standard (ThermoFisher Scientific ...
-
bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
-
bioRxiv - Microbiology 2022Quote: ... The supernatants of Expi293™ cells transfected with pAd/SARS-CoV-2-S1SA was diluted 1:40 in PBS-T with 1% BSA and along with standard control protein 40591-V08H (rS1H, Sino Biological) or purified rSARS-CoV-2S1Beta were incubated overnight at 4°C ...
-
bioRxiv - Immunology 2022Quote: ... Various dilutions of SEC-purified S-EABR eVLP samples and known amounts of soluble SARS-CoV-2 S1 protein (Sino Biological) were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare) ...
-
bioRxiv - Microbiology 2023Quote: ... IHC was carried out using a rabbit monoclonal antibody for SARS-CoV nucleocapsid protein (catalogue #40143-R001, clone #001, Sino Biological) which cross-reacts with SARS-CoV-2 nucleocapsid protein ...
-
bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
-
Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... and then membrane incubated with mouse anti-influenza A virus NP protein monoclonal Ab (Sino biological Company, China; 1: 10,000, 1 h) or mouse anti-influenza polyclonal anti-body (1 ...
-
bioRxiv - Biochemistry 2020Quote: ... the “BiEV index (caspase 14)” was used based on the fluorescein-labeled recombinant caspase 14 protein (11856-H07E; Sino Biological, Beijing, China) labeled with NHS-fluorescein (Thermo Fisher Scientific ...
-
Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: ... then proteins were monitored with ECL Western blotting substrate (Pierce) after incubation with anti-SARS-CoV-2-S-RBD (Sino Biological, China) or anti-β-actin antibody (Solarbio ...
-
bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
-
bioRxiv - Immunology 2020Quote: ... The expression of SARS-CoV-2 S protein was analyzed by Western blotting with rabbit polyclonal antibody to SARS-CoV-2 RBD (Sino Biological, China) and heat-inactivated human serum samples from Chinese COVID-19 infected patients ...
-
bioRxiv - Bioengineering 2021Quote: ... were coated with 100 mM bicarbonate/carbonate buffer (pH 9.6) containing 0.03 μg/mL of recombinant proteins (VP1) from homologous wild-type viruses (Sino Biological Inc., Beijing, China) overnight at 4 °C ...
-
bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
-
bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the SARS-CoV and SARS-CoV-2 S proteins were purchased from Sino Biologicals (catalog # VG40150-G-N; VG40589-CY). Expression of different coronavirus E proteins ...
-
bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
-
bioRxiv - Bioengineering 2023Quote: ... The libraries were subjected to five rounds of biopanning against the recombinant SARS-CoV-2 BA.1 RBD protein (Sino Biological Inc.) as described previously38 ...
-
bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 RBD fusion protein with the Fc region of mouse IgG1 at the C-terminus (RBD-Fc) was purchased from Sino Biological (Beijing, China), recombinant SARS-CoV-2 RBD with a C-terminal His-tag was purchased from Kactus Biosystems (Shanghai ...
-
bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
-
bioRxiv - Microbiology 2020Quote: ... For the detection of SARS-CoV-2 spike protein a rabbit polyclonal anti-SARS-CoV-2 spike S2 antibody (Sino Biological #40590-T62) was used.
-
bioRxiv - Immunology 2021Quote: ... Potency was assessed using a cell-based immunosorbent assay to quantify infection by detecting the SARS-CoV-2 nucleoprotein using a specific antibody raised against this protein (Sino Biological, Wayne, PA). The secondary antibody (Life Technologies ...
-
bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
-
bioRxiv - Immunology 2021Quote: ... and incubated the monolayer with the rabbit polyclonal antibody specific to SARS-CoV-2 RBD protein (1:200) (cat. n° 40592-T62, Sino Biological, Beijing, China), and a chicken antiserum specific to Newcastle disease virus (1:200 ...
-
bioRxiv - Microbiology 2021Quote: Biotinylated SARS-CoV-2 Spike D614G protein (Spikebiotin, in-house generated) or the biotinylated SARS-CoV-2 Spike receptor-binding domain (RBDbiotin, Sino Biological, 40592-V08B) were incubated with Alexa Fluor® 647 streptavidin (AF647-strep ...
-
bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
-
bioRxiv - Microbiology 2022Quote: ... spleen and tracheobronchial lymph node were covered with a mouse monoclonal anti-SARS-CoV nucleocapsid protein (#40143-MM05, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at room temperature for forty five minutes ...
-
bioRxiv - Microbiology 2022Quote: ... followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher, #26183) or an antibody against SARS-CoV Spike protein (Sino Biologicals, #40590-T62).
-
bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
-
bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
-
bioRxiv - Immunology 2021Quote: ... sections were stained with an anti-SARS-CoV/SARS-COV-2 nucleocapsid (N) protein rabbit monoclonal antibody (#40143-R001, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at RT for 45 min ...
-
bioRxiv - Immunology 2021Quote: ... blots were incubated with an anti-SARS-CoV spike protein polyclonal antibodies (S1, Sino Biological #40591-T62; S2, Invitrogen #PA1-41165; RBD, Sino Biological #40592-MP01) then visualized with horseradish peroxidase (HRP)-conjugated anti-mouse or anti-rabbit IgG (Bethyl ...
-
bioRxiv - Immunology 2020Quote: ... RBD219-N1C1 was detected using a rabbit monoclonal antibody against the SARS-CoV-2 Spike S1 protein (Sino Biological, Beijing, China; Cat#: 40150-R007) and goat anti-rabbit IgG secondary antibodies conjugated with horseradish peroxidase (Invitrogen ...
-
bioRxiv - Immunology 2021Quote: ... Cells were fixed with 4% PFA and were incubated with either a polyclonal antibody against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or a monoclonal antibody against the SARS-CoV-2 S1 protein (MyBioSource ...
-
bioRxiv - Bioengineering 2023Quote: ... The sample solutions were prepared by spiking biotinylated SARS-CoV-2 N protein rabbit monoclonal antibody (no. 40143-R004-B; Sino Biological, Inc.; Beijing, China) in buffer solution (0.1% BSA and 0.05% Tween 20 in PBS ...