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Citations for Sino Biological :
151 - 200 of 475 citations for IL 1 Alpha Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Androgen Regulates SARS-CoV-2 Receptor Levels and Is Associated with Severe COVID-19 Symptoms in MenbioRxiv - Cell Biology 2020Quote: ... 0.5 μM/mL human Fc-tagged recombinant spike-RBD protein with (Sino Biological Inc. 40592-V02H) was added to the medium and incubated for 30 minutes ...
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bioRxiv - Microbiology 2019Quote: ... 2.5 µl [62.5 ng/ul] of human Argonaute 2 (hAgo2) protein (Sino Biologicals, North Wales, PA) and 15 µl of Assembling Buffer [2 mM Mg(OAc)2 ...
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bioRxiv - Molecular Biology 2021Quote: ... or recombinant human ACE2-hIgG1 (2 μg/ml, diluted in blocking buffer, Sino Biological, 10108-H02H) were added for 1h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Human IL1RL1/ST2 Protein (isoform a, His Tag) (Cat. #13034-H08H) was purchased from Sino Biological. NF-κB inhibitor Bay 11-7802 and JAK inhibitor 1 were both from EMD Millipore ...
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bioRxiv - Synthetic Biology 2023Quote: ... The recombinant human angiotensin-converting enzyme 2 (rhACE2) was purchased from Sino Biological (Cat# 10108-H08H).
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bioRxiv - Neuroscience 2024Quote: ... 200 ng or the indicated amount of human recombinant PGRN (rPGRN, # 10826-H08H, Sino Biological Inc.) was either added to the activation assay or to the activity assay.
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were transiently transfected with a plasmid expressing human DC-SIGN (HG10200-UT; Sino Biological), using Promega FuGENE™ HD Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human KRAS (G12D) and the corresponding His tag (12259-H07E1) were purchased from Sino Biological (Beijing, China). Human KRAS (G12V) ...
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bioRxiv - Bioengineering 2023Quote: ... Human polymeric immunoglobulin receptor (hpIgR) construct was assembled from a cDNA clone (Sino Biological, cat. HG10131-UT), which was used to extract the fragment encoding the hpIgR ectodomain ( GenBank:NP_002635 ...
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bioRxiv - Molecular Biology 2024Quote: ... Glass coverslips (24 × 60 mm) were coated with human recombinant Fas protein (50 μg/mL, Sino Biological) at a defined area (10 × 10 mm) ...
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bioRxiv - Bioengineering 2020Quote: ... Human-cell-expressed SARS-CoV-2 Spike S1-His recombinant protein was provided by Sino Biological (40591-V08H). The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337) ...
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bioRxiv - Cancer Biology 2020Quote: ... The pCMV3 plasmid containing C-terminal His-tagged human GOT2 cDNA was purchased from Sino Biological (HG14463-CH) and cloned into the lentiviral vector pLenti CMV Puro DEST (Addgene #17452 ...
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bioRxiv - Cancer Biology 2019Quote: The vector encoding human Gal-14 gene (HG11371-CH) was obtained from Sino Biological (North Wales, PA, USA). The plasmid encoding human Gal-7 gene was obtained through the cloning of galectin-7 cDNA into sr⍺ eukaryotic expression vector using SpeI and BamHI restriction enzymes as previously described (Campion et al ...
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bioRxiv - Microbiology 2021Quote: ... we screened preconstructed human naïve phage scFv libraries with biotinylated SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with human recombinant TGF-β1 (5 ng/ml, 10804-HNAC, Sino Biological Inc., Beijing, China), CMLD-2 (LN03203514 ...
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bioRxiv - Bioengineering 2023Quote: Full length cDNA of human CD123 (NM_002183.2) was obtained from a pCMV3 vector (Cat#HG10518-M, Sino Biological). The Hygromycin sequence was replaced by a Neomycin resistance cassette by Gibson Assembly ...
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bioRxiv - Genetics 2020Quote: Mammalian cDNA ORF clones were used for AVP (human, HG17671-UT, NCBI Ref Seq: NM_000490.4, Sino Biological, Beijing, China) and MAOA (mouse ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Chi3l1-/- mice treated with APAP were immediately injected (i.p.) with either PBS (100μl) or recombinant human Chi3l1 (rhChi3l1, 1μg/mouse in 100μl, Sino Biological 11227-H08H). After 3h ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments encoding human hSSB1 and INTS3 were amplified by PCR from NABP2- GFPspark plasmid (Sino Biological, HG22790-ACG) and INTS3- GFPspark plasmid (Sino Biological ...
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bioRxiv - Immunology 2023Quote: ... A standard curve for calculations of unknowns was constructed using recombinant human TREM2 (4000–62.5 pg/mL, Sino Biological), and samples were diluted 1:4 before being assayed
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bioRxiv - Cancer Biology 2023Quote: ... was coated overnight with 100 ng/well (in 100 μL/well volume) of purified human CEACAM6 protein (Sino Biological). Standards containing known concentrations of the UCHT-2-03 recombinant protein or test samples were applied to the plate for 1 hour ...
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bioRxiv - Cell Biology 2023Quote: ... N-terminally myc-tagged human NOLC1 cDNA (GenBank ID NM_001284388.2) expression plasmid was procured from Sino Biologicals (HG16317-NM). cDNA encoding human IP6K1 domains ...
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bioRxiv - Immunology 2022Quote: ... The spike expression on cell surface were detected by staining cells with human ACE2–Fc chimera (Sino Biological, 10108-H02HG) in MACS buffer (D-PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Biochemistry 2021Quote: Vero E6 cells were incubated with RBD protein (0.45 μg/mL, final concentration) followed by incubation with human anti-RBD antibody (primary antibody) (40150-D003, Sino Biological) and goat anti-human IgG AF488-conjugated antibody (secondary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by applying 100 ng/well (in 100 μL/well volume) of Flag-tagged human CD3δ/ε heterodimer (Sino Biological) in 100 μL of Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Full-length monoclonal antibodies (mAb) and antibody fragments (Fab) were produced recombinantly in human cells by Sino Biological (Wayne, PA). Fabs were made from mAbs by papain protease digestion ...
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bioRxiv - Immunology 2019Quote: ... 0.23 nM LUVs with Bodipy-PE were then incubated with 8.3 nM human PD-L1-His (Sino Biological, catalog 10084-H08H) for 90 minutes in PBS with 1 mg/mL BSA at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Myc epitope-tagged SARS-CoV-2 (2019-nCoV) spike S cDNA (VG40589-CM) and Flag epitope-tagged human ACE2 cDNA (HG10108-NF) plasmids were purchased from Sino Biological.
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bioRxiv - Biochemistry 2020Quote: A Pro-CTSL clone was generated by sub-cloning the human Pre-Pro-CTSL ORF clone (in pMD18-T Simple Vector) obtained from Sino Biologicals Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA of human CD47 and mouse CD47 with an HA-tag at the C-terminal were purchased from Sino Biological and Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: Purified recombinant SARS-CoV-2 S1 subunit including a histidine tag and the RBD tagged with the Fc region of human IgG1 (both Sino Biological) were used in ELISA to detect SARS-CoV-2 S-specific IgG in mice ...
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bioRxiv - Microbiology 2021Quote: The rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Cat# 40592-T62) or human SARS-CoV-2 S-NTD antibody (E-AB-V1030) was obtained from Sino Biological or Elabscience ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Evolutionary Biology 2023Quote: The plasmid containing the human SLC30A9 gene codifying for the ZnT9 transporter with an N-HA tag was obtained from Sino Biological Inc ...
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bioRxiv - Immunology 2023Quote: ... and blocking activity of anti-CTLA-4 monoclonal antibodies were determined by ELISA using recombinant human B7.1 (Sino Biological, #10698-HCCH), human CTLA-4 (Sino Biological ...
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Cancer Biology 2021Quote: pCMV3-N-HA negative control vector and human RPRM plasmids (full length, S98A mutant and Δ(79-109) mutant) were purchased from Sino Biological Inc ...
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bioRxiv - Bioengineering 2020Quote: ... Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological ...
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bioRxiv - Cancer Biology 2020Quote: ... Prepared elastic Fab anti-Fc-functionalized PAA nanotextures were incubated with 20 μg/mL human ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (4°C ...
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bioRxiv - Biochemistry 2021Quote: Affinity-purified full-length EGFR was concentrated to 50 μM and treated with 100 μM human EGF (Novoprotein) or TGF-α (Sino Biological) for at least 5 mins on ice ...
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bioRxiv - Microbiology 2022Quote: ... the PBMCs were cultured for 7 days in presence of 25ng/mL recombinant human macrophage colony-stimulating factor (rh-MCSF, Sino Biological, China). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Neuroscience 2019Quote: ... CSF and plasma sTREM2 concentrations were calculated using the standard curve generated for each plate using recombinant human TREM2 protein (Sino Biological, Wayne, PA). A dedicated CSF and plasma sample was loaded onto all plates and used to normalize values ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Microbiology 2021Quote: ... human (WT or H34Y) or mink ACE2 (WT or Y34H) were incubated with rabbit polyclonal antibody (Sino Biological Inc. China, Cat: 10108-T24) against ACE2 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were firstly pre-treated with the C5aR1 antagonist PMX53 (10 μM) for 20min (37 ℃, 5 % CO2) before stimulated with recombinant human C5a (Sino Biological, Beijing, China) or P32 (100 μM ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) VWF-bound gcEVs (VWF+gcEVs) obtained by incubation of gcEVs with 10 µg of recombinant (r) human VWF (Sino Biological, Beijing, China); (3 ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-Human Fc FAB patterns on PAA were incubated with 10 µg/mL ICAM1-hFc solution (Sino Biological, Cat# 10346-H03H-50) and α-collagen-1 Ab micropatterns on PAA gels were incubated with 1 mg/mL rat monomeric collagen-1 (Corning ...