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Citations for Sino Biological :
351 - 400 of 407 citations for Human integrin alpha 5 beta 3 His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Islets in Akura™ 96 Spheroid Microplate were treated with 0.3 ng/mL or 30 ng/mL of human recombinant IL-1R2 protein (10111-H08H, Sino Biological) for 16 days ...
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bioRxiv - Cancer Biology 2023Quote: ... followed by applying 100 ng/well (in 100 μL/well volume) of Flag-tagged human CD3δ/ε heterodimer (Sino Biological) in 100 μL of Dulbecco’s phosphate-buffered saline (DPBS) ...
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bioRxiv - Biochemistry 2024Quote: ... Full-length monoclonal antibodies (mAb) and antibody fragments (Fab) were produced recombinantly in human cells by Sino Biological (Wayne, PA). Fabs were made from mAbs by papain protease digestion ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001 ...
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bioRxiv - Biochemistry 2020Quote: ... Then 5 μL of 2019-nCoV spike pseudotyped virus was added (Sino Biological, PSC001), and incubated in a 37 °C incubator containing 5% CO2 for 4 h followed with adding 100 μL of complemented DMEM per well ...
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bioRxiv - Biochemistry 2020Quote: The recombinant forms of human ACE2 (residues 1-740) and RBD (residues 319-541) were purchased from Sino Biological (Wayne, PA). Both proteins were extensively dialyzed in 150 mM NH4CH3CO2 prior to MS analyses ...
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bioRxiv - Neuroscience 2020Quote: ... Myc epitope-tagged SARS-CoV-2 (2019-nCoV) spike S cDNA (VG40589-CM) and Flag epitope-tagged human ACE2 cDNA (HG10108-NF) plasmids were purchased from Sino Biological.
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bioRxiv - Biochemistry 2020Quote: A Pro-CTSL clone was generated by sub-cloning the human Pre-Pro-CTSL ORF clone (in pMD18-T Simple Vector) obtained from Sino Biologicals Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: The rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Cat# 40592-T62) or human SARS-CoV-2 S-NTD antibody (E-AB-V1030) was obtained from Sino Biological or Elabscience ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Immunology 2023Quote: ... and blocking activity of anti-CTLA-4 monoclonal antibodies were determined by ELISA using recombinant human B7.1 (Sino Biological, #10698-HCCH), human CTLA-4 (Sino Biological ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Immunology 2022Quote: ... or 0.13 μg biotinylated SARS-CoV-2 BA.5 RBD protein (customized from Sino Biological) conjugated with PE-streptavidin (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... intramuscularly (IM) with 5 μg of SARS-CoV-2 Spike S1 (Sino Biological 40592-V05H) protein with or without adjuvants ...
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bioRxiv - Cancer Biology 2021Quote: pCMV3-N-HA negative control vector and human RPRM plasmids (full length, S98A mutant and Δ(79-109) mutant) were purchased from Sino Biological Inc ...
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bioRxiv - Bioengineering 2020Quote: ... Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological ...
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bioRxiv - Cancer Biology 2020Quote: ... Prepared elastic Fab anti-Fc-functionalized PAA nanotextures were incubated with 20 μg/mL human ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (4°C ...
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bioRxiv - Biochemistry 2021Quote: Affinity-purified full-length EGFR was concentrated to 50 μM and treated with 100 μM human EGF (Novoprotein) or TGF-α (Sino Biological) for at least 5 mins on ice ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-1 pseudotyped virus neutralization assay) or 293T cells overexpressing human angiotensin-converting enzyme 2 (293T-hACE2) (Sino Biological Company) (for Pangolin-GD and RaTG13 pseudotyped virus neutralization assay) ...
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bioRxiv - Microbiology 2022Quote: ... the PBMCs were cultured for 7 days in presence of 25ng/mL recombinant human macrophage colony-stimulating factor (rh-MCSF, Sino Biological, China). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Biochemistry 2020Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc.) for 30 min before transfection ...
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bioRxiv - Immunology 2021Quote: ... ~ 5×10e5 PBMC were stained with 100 ng of full length biotinylated spike protein (Sino Biological) pre-incubated with Streptavidin-BV510 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... The wells were coated with 5 μg/ml of the spike protein (Sino Biological, PA, USA) overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... CSF and plasma sTREM2 concentrations were calculated using the standard curve generated for each plate using recombinant human TREM2 protein (Sino Biological, Wayne, PA). A dedicated CSF and plasma sample was loaded onto all plates and used to normalize values ...
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bioRxiv - Neuroscience 2019Quote: TSA-201 cells cultured at ~60% confluency were transfected with 1 ug/mL human MUC1 in pCMV3-GFPSpark (Sino Biological, Collegeville, PA; MG50877ACG) using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: ... human (WT or H34Y) or mink ACE2 (WT or Y34H) were incubated with rabbit polyclonal antibody (Sino Biological Inc. China, Cat: 10108-T24) against ACE2 ...
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bioRxiv - Synthetic Biology 2022Quote: ... were coated overnight at 4 °C with 50 μL of 5-μg/mL in-house prepared ColE7 in PBS for ribosome-tethered Im7N58 or the same amount of commercial extracellular domain (residues 1 to 652) of human HER2 (HER2-ED; Sino Biological, 10004-HCCH) for ribosome-tethered scFv–HER2DQNAT in PBS ...
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bioRxiv - Neuroscience 2022Quote: ... and -Spike Subunit 1 Antibody (Monoclonal Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:100, Clone 007, Sino Biological, Code Number: 40150-R007) immunostainings were employed to evaluate viral antigens within the tissue ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) VWF-bound gcEVs (VWF+gcEVs) obtained by incubation of gcEVs with 10 µg of recombinant (r) human VWF (Sino Biological, Beijing, China); (3 ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated for 1 hour with serially diluted IgGs with 1µg/mL of human CTLA-4-Biotin (Sino Biological, #11159-H08H-B) in 1xPBS with 2% BSA ...
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bioRxiv - Cell Biology 2023Quote: ... the anti-Human Fc FAB patterns on PAA were incubated with 10 µg/mL ICAM1-hFc solution (Sino Biological, Cat# 10346-H03H-50) and α-collagen-1 Ab micropatterns on PAA gels were incubated with 1 mg/mL rat monomeric collagen-1 (Corning ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed a further 3 times with 300 μL PBSB prior to the addition of 100 ng/mL BiotinylatedACE2 (Sino Biological) in PBSB + 1% casein (50 μL/ well ...
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bioRxiv - Immunology 2021Quote: ... The gradient concentrations of SARS-CoV-2 S1 or an artificial chimeric construct carrying 3 mutations on B.1.351 RBD and SD614G (B.1.351 S1) (Sino Biological, Beijing, China) were prepared (2-fold dilutions ...
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bioRxiv - Immunology 2020Quote: ... The plate was washed for 3 times then incubated with mouse sera and SARS-CoV-2 Spike antibody (Sino Biological, China) for 2 hours at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Immunology 2022Quote: ... were maintained at 37°C in 5% CO2 in SMM 293-TII expression medium (Sino Biological, M293TII). Sf9 cells (ATCC ...
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bioRxiv - Immunology 2022Quote: ... BA.2.12.1 (Acro SPN-C522d-50ug) and BA.4/5 (SPN-C5229-50ug) were purchased from Sino Biological and AcroBiosystems ...
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bioRxiv - Immunology 2022Quote: ... BA.2.12.1(Acro SPD-C522q-100ug) and BA.4/5(Acro SPD-C522r-100ug) were purchased from Sino Biological and AcroBiosystems ...
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bioRxiv - Bioengineering 2019Quote: ... cells were detached with 50 mM EDTA and 5 × 105 cells were stained with rabbit anti-CEA antibody (Sino Biological) (500 nM ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: ... containing 10 μg OVA or 5 μg of the spike protein of SARS-CoV-2 (the receptor-binding domain; recombinant product from Sino Biological) mixed with or without 10 μg or 20 μg S100A4 as indicated ...
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bioRxiv - Microbiology 2022Quote: ... a total of 5 × 105 splenocytes in a volume of 200 µL was stimulated with the full-length S peptide mix (Sino Biological) (0.1 μg/mL final concentration per peptide) ...
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bioRxiv - Immunology 2020Quote: ... Association curves were recorded for 5 minutes by incubating the mAb-coated sensors with different concentration of SARS-CoV RBD (Sino Biological) or SARS-CoV-2 RBD (produced in house ...
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bioRxiv - Cancer Biology 2023Quote: ... the conditioned media from treated HUVECs were centrifuged at 3000×g for 5 min and the supernatant was analyzed for VWF:Ag using ELISA (Sino Biological Inc.). For gcEV binding ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells were starved for 5 hours in DMEM/F12 supplemented with 1% PS and then stimulated with 100ng/mL BDNF (50240-MNAS, Sino Biological) for different time periods (5 ...
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bioRxiv - Biochemistry 2021Quote: ... C5aR1−/− and C5aR2−/− mice on a C57BL/6J genetic background (n=5-15) were administered with recombinant mouse C5a (Sino Biological, China) at a dose of 50 μg kg-1 via intravenous injection (tail vein) ...