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Citations for Sino Biological :
101 - 150 of 168 citations for African Swine Fever Virus P30 Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were transiently transfected with a plasmid expressing human DC-SIGN (HG10200-UT; Sino Biological), using Promega FuGENE™ HD Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (Sino Biological) using FuGENE 6 (Promega) ...
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bioRxiv - Immunology 2020Quote: ... Baculovirus-expressed S2 subdomain and HEK293 cell-expressed N protein were obtained from Sino Biological (Chesterbrook, PA) and RayBiotech (Peachtree Corners ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK293T cells were grown to 80% confluency before transfection with pCMV3-SARS-CoV-2-spike (Sino Biological) using Lipofectamine®3000 (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with anti-SARS-CoV-2 Spike protein antibodies (1:200, Sino Biological, 40150-R007) overnight at 4 °C ...
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bioRxiv - Biophysics 2023Quote: ... Sf9 cells were cultured in suspension at 27 °C in SIM SF culture medium (Sino Biological, China) and routinely passaged every other day ...
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bioRxiv - Bioengineering 2020Quote: ... Human-cell-expressed SARS-CoV-2 Spike S1-His recombinant protein was provided by Sino Biological (40591-V08H). The human-cell-expressed SARS-CoV Spike S1-His recombinant protein was purchased from Creative Biolabs (VAng-Wyb7337) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed and stained with a polyclonal rabbit anti-SARS-CoV antibody (Sino Biological; 1:500). Staining was developed using a rabbit anti-SARS-CoV serum and a secondary alexa-fluor-labeled conjugate (Dako) ...
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bioRxiv - Microbiology 2020Quote: ... Cells were incubated with 200 µl primary antibody (rabbit anti-SARS-CoV-2 N, Sino Biological, 40143-R019) diluted 1:1000 in blocking buffer (4% dried milk/0.1% Tween-20 in PBS ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with a primary antibody targeting SARS-CoV-2 nucleocapsid protein (Sino Biological, cat. 40143-R001) at a 1:2000 dilution for 1h ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004) diluted 1:5000 in PBS with 1% bovine serum albumin and 0.2% skim milk powder (PBSK ...
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bioRxiv - Microbiology 2021Quote: ... the proportion of transfected cells was determined by staining with biotinylated RBD (Sino biologicals, Cat: 40592-V08H-B) for 30 min ...
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bioRxiv - Immunology 2020Quote: ... Fixed and permeabilized cells were first stained with a primary antibody recognizing SARS-CoV nucleocapsid protein (Sino Biological), followed by secondary antibody staining with AlexaFluor 488-conjugated goat anti-rabbit antibody ...
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bioRxiv - Immunology 2020Quote: ... and the number of infected cells was determined using SARS-CoV-S specific mAb (Sino Biological 401430-R001) and fluorescently labeled secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... Cells were also stained with SARS-CoV-2 antigens including biotinylated SARS-CoV-2 RBD protein (Sino Biological) and full-length SARS-CoV-2 Spike protein (Sino Biological ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with human recombinant TGF-β1 (5 ng/ml, 10804-HNAC, Sino Biological Inc., Beijing, China), CMLD-2 (LN03203514 ...
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bioRxiv - Microbiology 2023Quote: Cells were then incubated overnight at 4°C with rabbit anti-HCoV-229E nucleocapsid (1:400, Sino Biological) diluted in 1×SEA BLOCK ...
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bioRxiv - Cell Biology 2022Quote: ... the cell lysates were incubated with 50 μl of Protein A agarose beads (Sino Biological, Chesterbrook, PA, USA) at 4°C for another 2 h ...
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bioRxiv - Microbiology 2022Quote: ... the medium of the transfected cells was replenished with the SMM 293-TII Expression Medium (Sino Biological, M293TII), and the protein-containing supernatant was harvested every three days for 2-3 batches ...
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bioRxiv - Microbiology 2024Quote: ... Cells were then stained with polyclonal anti-SARS-CoV-2 S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) followed by anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Microbiology 2024Quote: ... The transfected cells were cultured in SMM 293-TIS Expression Medium (Serum-free, without L-Glutamine) (Sino Biological). The supernatant ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed and incubated in primary conjugated antibodies (EZH2, BD Bioscience; CD133, Miltenyi; CD15, Biolegend; Sox2, Sino Biological Inc. ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% formaldehyde in PBS and stained with polyclonal rabbit anti-SARS-CoV Ab (Sino Biological) and secondary Alexa488 conjugated goat-anti-rabbit Ab (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Microbiology 2023Quote: ... the culture medium of the transfected cells was replaced with the SMM 293-TII Expression Medium (Sino Biological, M293TII) 4-6 hours post-transfection ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were stained in FACS buffer with anti-CEACAM6-FITC antibody (Sino Biological 10823-R408R, Sino Biological, Inc., Wayne, PA) at 10 μg/mL for 30 min at 4°C in the dark ...
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bioRxiv - Biochemistry 2024Quote: ... Full-length monoclonal antibodies (mAb) and antibody fragments (Fab) were produced recombinantly in human cells by Sino Biological (Wayne, PA). Fabs were made from mAbs by papain protease digestion ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were pelleted and resuspended in incubation buffer containing increasing concentrations of recombinant FC-ACE2 (Sino Biological Cat# 10108-H05H-100) followed by incubation for 20 minutes on ice ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Genetics 2020Quote: ... cells were treated with 2 ng/ml of recombinant biotinylated SARS-CoV-2 spike protein RBD (spike protein-RBD, Sino Biological) for 1 hour at 37°C ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... The cell culture supernatants were collected and analyzed by western blot analysis using a commercial antibody (Sino Biological Inc. Beijing, China) against SARS-CoV-2 RBD.
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bioRxiv - Immunology 2022Quote: Plasma or memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2023Quote: ... Antigen-positive cells were detected by indirect immunofluorescence assay (IFA) using an anti-HCoV-229E nucleoprotein antibody (Sino Biological, Beijing, China).
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Microbiology 2023Quote: ... the SARS-CoV2 infected cells were fixed and immunostained with a SARS-CoV Nucleocapsid Antibody (Sino Biological, Catalogue number: 40143-MM05), followed by HRP-conjugated Goat anti-Mouse IgG (H+L ...
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bioRxiv - Immunology 2023Quote: Memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Immunology 2021Quote: ... Cells were stained with an anti-SARS antibody (SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit monoclonal antibody; Sino Biological #40143-R001), diluted to 0.125 μg/mL in 3% BSA/PBS blocking solution for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... ID8 and SK-OV-3 cells were stimulated with 2.5 ng/mL murine and human recombinant IL-20 protein (Sino Biological, Beijing, China) for 24 hours ...
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bioRxiv - Immunology 2020Quote: Internalization of CCR7 was also examined with HEK293T cells stably expressing a mouse CCR7-GFP fusion construct (pCMV3-mCCR7-GFPSpark, Sino Biological Inc.). HEK293T cells were transfected with CCR7-GFP with Lipofectamin 2000 (Gibco) ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Neuroscience 2019Quote: TSA-201 cells cultured at ~60% confluency were transfected with 1 ug/mL human MUC1 in pCMV3-GFPSpark (Sino Biological, Collegeville, PA; MG50877ACG) using Lipofectamine 3000 (Thermo Fisher) ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...