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Citations for Sino Biological :
101 - 150 of 154 citations for 7 S 17 S dihydroxy 8 E 10 Z 13 Z 15 E 19 Z Docosapentaenoic Acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... The expression of SARS-CoV-2 S protein was analyzed by Western blotting with rabbit polyclonal antibody to SARS-CoV-2 RBD (Sino Biological, China) and heat-inactivated human serum samples from Chinese COVID-19 infected patients ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the SARS-CoV and SARS-CoV-2 S proteins were purchased from Sino Biologicals (catalog # VG40150-G-N; VG40589-CY). Expression of different coronavirus E proteins ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 S expression plasmid pC-SARS2-S was created by inserting the Acc65I/NotI-digested PCR-amplified SARS-CoV-2 S fragment of pCMV3-2019-nCoV-Spike(S1+S2)-long (Sino Biological; VG40589-UT) into the corresponding site of pCAGGS ...
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bioRxiv - Microbiology 2020Quote: Sections were covered with rabbit polyclonal anti-SARS-CoV S antibody diluted at 1:250 (Sino Biologicals, #40150-T62-COV2, Supplemental Table 1) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Microbiology 2023Quote: ... groups of five 8-to-12-week C57B6 mice (Taconic Farms Inc) were immunized with 4 μg/mouse of HCoV-OC43 S-His (Sino Biological # 40607-V08B) or N-His protein (Sino Biological # 40643-V07E ...
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bioRxiv - Microbiology 2020Quote: A full-length wild-type pCMV3-SARS-CoV-2 Spike (S1+S2)-long (termed as pCMV-S, codon-optimized, Sino Biological, cat # VG40589-UT) plasmid was used as a template to generate tagged pCMV-S ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...
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bioRxiv - Bioengineering 2022Quote: ... IL-8 (Sino Biological, Wayne, PA) at 1000 μg/mL ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (pH 6.0) for 12 min for SARS-CoV-1 nucleoprotein antibody (Sino Biological Inc.; Beijing, China) and using recombinant protease from Streptomyces griseus (PanReac Applichem ...
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bioRxiv - Microbiology 2022Quote: ... or 8 μg empty vector (Sino Biologicals, cat # CV011). Cells were harvested 120 h post-transfection for Western blot to assess overexpression efficiency.
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bioRxiv - Immunology 2022Quote: ... The human IL-15 ELISA Pair Set (Sino Biological) was used for the quantitative determination of IL-15 monomer.
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bioRxiv - Immunology 2023Quote: ... and Fc-tagged DLL4 (15 μg/ml; Sino Biological, 10171-H02H). Prior to seeding ...
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bioRxiv - Immunology 2021Quote: ... and 200 μg per dose of recombinant human IL-15 (Sino Biological). One year later ...
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bioRxiv - Immunology 2021Quote: ... and influenza virus A/PR/8/34 H1N1 hemagglutinin (HA) protein (Sino Biological, 11684-V08H) were coated at a concentration of 2 μg/ml in carbonate buffer on 96-well MaxiSorp plates (Thermo Fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... or exposed to 15 pg/ml recombinant human interleukin-1β (IL-1β, Sino Biological Inc.®) or 10 ng/ml recombinant rat IFNγ (R&D® ...
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bioRxiv - Pathology 2022Quote: Recombinant His-tagged TRP120 (10 μg) and Notch-1 (10 μg) (Sino Biological) were incubated with Ni-NTA beads alone ...
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bioRxiv - Microbiology 2022Quote: ... the PBMCs were cultured for 7 days in presence of 25ng/mL recombinant human macrophage colony-stimulating factor (rh-MCSF, Sino Biological, China). Cells were cultured at 37°C in a humidified atmosphere with 5% CO2.
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bioRxiv - Neuroscience 2023Quote: ... and human HepaCAM protein extracellular domain (ECD, amino acid sequence 1-240, 16047-H08H) (Sino Biological Inc.) were used in this study ...
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bioRxiv - Microbiology 2022Quote: ... Codon optimized SARS-CoV-2 Wuhan Spike carrying the D614G amino acid change (Sino Biological #VG40589-UT(D614G)) was modified to remove the last 21 amino acids at the C-terminus (SpikeΔ21 ...
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bioRxiv - Cell Biology 2022Quote: ... IL-1β (Sino Biological, 10 ng/mL), Rot/A (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 8 μg/ml SARS-CoV-2 antigens (spike glycoprotein extracellular or receptor-binding domains, or nucleocapsid: Sino Biological, China) or SARS antigen (spike glycoprotein S1 subunit ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with the SARS-CoV-2 S1 subunit (amino acids 1–685) (Sino Biological, China) at 1 μg/ml in PBS (50 ul/well) ...
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bioRxiv - Biochemistry 2021Quote: ... C5aR1−/− and C5aR2−/− mice on a C57BL/6J genetic background (n=5-15) were administered with recombinant mouse C5a (Sino Biological, China) at a dose of 50 μg kg-1 via intravenous injection (tail vein) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml LIF (Sino Biological, 14890 -HNAE), 10 ng/ml bFGF (Sino Biological,10014-HNAE) ...
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bioRxiv - Cell Biology 2020Quote: ... 10 ng/ml bFGF (Sino Biological,10014-HNAE), 0.1 mM β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... After stimulated by 10 ng/mL TNFα (Sino Biological), cells expressing high level of eGFP were sorted by FACS and the Jurkat/NF-κB-eGFP cell line were characterized ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Immunology 2022Quote: ... Mice serum was 3-fold diluted starting from 1:15 (Omicron) or 1:45 (WT) in the blocking buffer with a final amount of 50 ng biotinylated hACE2 (Sino Biological, cat# 10108-H08H-B) / 8 ng epitope 3 nanobody / 8 ng epitope 4 nanobody at each concentration and then incubated with the plate at room temperature for 2 hrs ...
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bioRxiv - Immunology 2020Quote: ... or 1-10 μg of Spike RBD protein (Sino Biological, Cat ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were treated with either 10 ng/ml IL-1β (Sino Biological) or mock-treated ...
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bioRxiv - Immunology 2020Quote: ... with 10 μg Influenza A H1N1 nucleoprotein (Sino Biological Inc., 11675-V08B) alone ...
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bioRxiv - Immunology 2023Quote: ... The cytokine IL22 (10 ng/ml, Cat. No. 13059-HNAE, Sino Biological) was used in certain experiments after transfection in some experiments.
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bioRxiv - Microbiology 2020Quote: ... Then 10 μL anti-FcγRI antibody-FITC (Cat: 10256-R401-F, Sino Biological), anti-FcγRIIa antibody-FITC (Cat ...
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bioRxiv - Cell Biology 2022Quote: ... and 10 ng/ml macrophage colony-stimulating factor (Sino Biological Inc., Beijing, China). The fresh medium containing 10 ng/ml macrophage colony-stimulating factor was changed every two days to induce differentiation of the cells into macrophages ...
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bioRxiv - Immunology 2023Quote: ... were coated with 10 μg/mL of H3 protein (Sino Biological, cat# 11715-V08H) in coating buffer (50 mM carbonate/ bicarbonate ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were labeled with the following antibodies:(CEACAM6-FITC at 10 μg/mL, Sino Biological 10823-R408R ...
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bioRxiv - Microbiology 2022Quote: Purified recombinant SARS-CoV-2 Spike (S1+S2) protein (10 μg/ml, Sino Biological, 40589-V08B1) exposed cells were fixed with 4% PFA for 15 minutes at RT ...
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bioRxiv - Cell Biology 2022Quote: ... in association with 10 ng/ml LPS and 20 ng/ml hIFNγ (Sino Biological Inc., Beijing, China) for 48 h ...
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bioRxiv - Microbiology 2022Quote: ... the culture medium was replenished with DMEM+10% FBS or SMM 293-TII Expression Medium (Sino Biological, M293TII) containing VSV neutralizing antibody (from I1-mouse hybridoma) ...
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bioRxiv - Immunology 2021Quote: ... streptavidin beads were coated as described above with the following biotinylated antigens: 10 µg/mL MERS spike (Sino Biological, 40069-V08B ...
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bioRxiv - Microbiology 2023Quote: ... the culture medium was replenished with either DMEM+10% FBS or SMM 293-TII Expression Medium (Sino Biological, M293TII), along with an antibody (from I1-mouse hybridoma ...
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bioRxiv - Immunology 2023Quote: ... donor mice (B6PL-Thy1.1) were hydrodynamically injected with 10 μg of a plasmid encoding murine IL-25 (pCMV3-mIL25, Sino Biological) as previously described (Frech et al ...
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bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... isolated splenocytes were cultured with 10 mg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) (Sino biological Europe). Recombinant Ovalbumin [10 mg/ml] served as negative protein control ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were firstly pre-treated with the C5aR1 antagonist PMX53 (10 μM) for 20min (37 ℃, 5 % CO2) before stimulated with recombinant human C5a (Sino Biological, Beijing, China) or P32 (100 μM ...