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Citations for Sino Biological :
851 - 900 of 908 citations for Human Immunodeficiency Virus Nef Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: Cells were then incubated overnight at 4°C with rabbit anti-HCoV-229E nucleocapsid (1:400, Sino Biological) diluted in 1×SEA BLOCK ...
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bioRxiv - Immunology 2024Quote: The antibodies used for immunoblotting include: rabbit anti-SARS-CoV-2 S2 (Sino Biological, 40590-T62, 1:2000), mouse anti-SARS-CoV-2 N (Sino Biological ...
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bioRxiv - Microbiology 2020Quote: ... DCs were pulsed with 20μg/mL SSP-1 peptide in the presence of 3μg/mL β-microglobulin (Sino Biological) at 37°C for 3 hours before use ...
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bioRxiv - Immunology 2021Quote: ... Recombinant SARS-CoV-2 spike protein S1 subunit (Wuhan-Hu-1 (Val16-Arg685) (accession YP_009724390.1)) with a C-terminal His tag was purchased from Sino Biological.
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bioRxiv - Microbiology 2021Quote: ... Immunofluorescence staining was conducted using the SARS-CoV-2 nucleoprotein mouse monoclonal antibody (1:500; Sino Biological #40143-MM05), Alexa-Fluor Plus 488 anti-mouse antibody (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... The recombinant spike trimers used in these assays were as follows: Wuhan-Hu-1 (Sino Biological, cat. 40589-V08H4), Delta (Sino Biological ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Immunology 2022Quote: ... were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Microbiology 2020Quote: ... were stained for 1 h with 0.01 mg/ml rabbit anti-SARS-CoV-2 Spike S1 subunit (clone#007, Sino Biologicals) and 0.03 mg/ml mouse anti-Tubulin IV (cloneONS.1A6 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 0777)/1 X DPBS % v/v solution (DPBST) and 50 µl/well of 0.05 µg/ml H3 (Sino Biological; 11231-RP02) or 0.5 µg/ml H3K4me3 (Millipore ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV were stained with mouse-anti-SARS-CoV nucleoprotein (40143-MM05, 1:400, Sino Biological) or rabbit-anti-SARS-CoV nucleoprotein (40143-T62 ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with the SARS-CoV-2 S1 subunit (amino acids 1–685) (Sino Biological, China) at 1 μg/ml in PBS (50 ul/well) ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilized with 70% ethanol and stained with polyclonal rabbit anti-SARS-CoV nucleoprotein antibody (1:1000; 40588-T62, Sino Biological) followed by secondary Alexa488 conjugated goat-anti-rabbit antibody (Invitrogen) ...
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bioRxiv - Bioengineering 2024Quote: ... 2X dilutions of the serum (starting at 1:50) were mixed with a constant 0.07 nM concentration of an HRP-conjugated anti-RBD monoclonal antibody (Sino Biological) and incubated for 2h at room temperature ...
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bioRxiv - Immunology 2024Quote: ... The antibodies and regents used for immunofluorescence include: rabbit anti-SARS-CoV-2 S2 (Sino Biological, 40590-T62, 1:200), mouse anti-SARS-CoV-2 N (Sino Biological ...
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Vaccinia virus-based vaccines confer protective immunity against SARS-CoV-2 virus in Syrian hamstersbioRxiv - Immunology 2021Quote: ... Immunohistochemical staining was performed with a monoclonal rabbit anti-SARS-CoV/SARS-CoV-2 nucleocapsid (NP) antibody (1:1000, 40143-R001, Sino Biological), followed by incubation with Dako EnVision+ System HRP ...
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bioRxiv - Immunology 2021Quote: ELISA plates were coated with RBD (SARS-CoV-2 WT, SARS-CoV-2 Omicron, SARS-CoV-1 RBD, Sino Biological Inc.) at 0.03 μg/mL and 1 μg/mL in PBS at 4°C overnight ...
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bioRxiv - Immunology 2022Quote: ... The Omicron BA.1 RBD and BA.2 RBD used in ELISA were purchased from Sino Biological (Cat. No. 40592-V08H121) and AcroBiosystems (Cat ...
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bioRxiv - Microbiology 2019Quote: ... and the A/H7 HA (EPI439507) and A/N9 NA (EPI439509) from A/Anhui/1/2013 (Anhui13) were purchased from Sino Biological. cDNAs were amplified by Platinum™ SuperFi™ PCR (Invitrogen ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... Plates were coated with 1 µg/ml of SARS-CoV-2 (2019-nCoV) spike RBD-His (Sino Biologicals, Cat. 40592-V08B) for 4-5h at 37°C and then blocked overnight in 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... IP complexes were sequentially eluted first with 50 ul of 1 mg/ml HA peptide (Sino Biological, catalog #PP100028, lot #IP15MA1401X) then boiling in sample buffer 95°C for 10 minutes.
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bioRxiv - Microbiology 2023Quote: ... using a combination of polyclonal antibodies against the viral spike protein (1:200 dilution of anti S1 RBD cat. no. 40592-T62, and 1:200 dilution of anti S2 cat. no. 40590-T62, both from Sino Biological), for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells were starved for 5 hours in DMEM/F12 supplemented with 1% PS and then stimulated with 100ng/mL BDNF (50240-MNAS, Sino Biological) for different time periods (5 ...
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bioRxiv - Immunology 2023Quote: ELISA assays were conducted by pre-coating ELISA plates with RBD (SARS-CoV-2 wild type, SARS-CoV-2 BA.1, SARS-CoV-2 BA.2 RBD, Sino Biological) at concentrations of 0.03 μg ml−1 and 1 μg ml−1 in PBS overnight at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... with rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc, Cat. no. 40592-T62, 1: 1000) and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2024Quote: ... and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike S2 Antibody (Sino Biological Inc, Cat. no. 40590-T62, 1: 1000) or N protein of SARS-CoV-2 (dilution 1:2000 ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... in 1 x TBST (Fisher AAJ77500K2) for 1 hour after the direct addition of 1.5 μl of either recombinant HER4 (Sino Biological 10363-H08H), or recombinant ATF-6 Beta (Abnova H00001388-Q01) ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times and then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 hour at 37 °C ...
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bioRxiv - Immunology 2021Quote: ... the viral nucleocapsid was detected using 1:5000 of SARS-CoV/SARS-CoV-2 Nucleocapsid monoclonal antibody (Sino Biological, Cat#40143-R001) by incubation at 37°C for 1 h ...
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bioRxiv - Microbiology 2024Quote: ... Samples were then incubated in a primary mouse SARS-CoV-2 nucleocapsid (N) antibody (Sino Biological Inc., Cat # 40588-T62, 1:1000) at 4 °C overnight ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were grown till 70-80% confluency and then treated for 48 hours with 1 μM recombinant mouse interferon gamma (Sino Biological, China). Treated cells were detached by trypsinisation and stained with antibodies against the MHC cell surface proteins.
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bioRxiv - Molecular Biology 2019Quote: ... Amount of Tf in plaque homogenates was also determined by western blot analysis using the anti-Tf antibody (1:2000, 11019-RP01, Sino Biological Inc, China) as described above.
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bioRxiv - Microbiology 2021Quote: ... Then the sections were incubated with primary antibodies: rabbit anti-SARS-CoV Spike (1:200, 40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse anti-SARS-CoV NP (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 1:500 dilution ratio of primary antibody specific for SARS-CoV nucleoprotein56 (Rabbit mAb) (Sino Biological Inc. China), which cross-reacting with the NP protein of SARS-CoV–2 as well ...
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bioRxiv - Immunology 2021Quote: ... When this was done the mix was marked with rabbit monoclonal antibody anti-SARS-CoV-2 S1 (1:200) (cat. n° 40150-R007, Sino Biological, Beijing, China) as primary antibody for 1h at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (pH 6.0) for 12 min for SARS-CoV-1 nucleoprotein antibody (Sino Biological Inc.; Beijing, China) and using recombinant protease from Streptomyces griseus (PanReac Applichem ...
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bioRxiv - Immunology 2020Quote: ... were coated with SARS-CoV-1 RBD-rCD4-His or SARS-CoV-2 RBD-rCD4-His or MERS-CoV S1-His (Sino Biological, 40069-V08H), at 3 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded lung were incubated for 1 hour with the primary antibodies SARS-CoV-2 nucleoprotein (mouse IgG1, catalog number 40143-MM08, Sino Biological, Wayne, PA), Ionized calcium-binding adaptor (IBA1 ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked and incubated with primary antibodies at 37℃ for 1 h to detect the S1 and S2 subunits using rabbit anti-SARS-Spike S1 (Sino Biological, 40591-T62) and rabbit anti-SARS-Spike S2 (Sino Biological ...
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bioRxiv - Immunology 2021Quote: ... were fixed after 48 hrs with 1:1 solution of methanol/ acetone and air-dried prior to immunostaining with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62; Sino Biological; 1:1000 dilution) and HRP-conjugated anti-Rabbit IgG antibody (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% skim milk in PBS-T and incubated with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62, Sino Biological; 1:1,000 dilution) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Then the sections were incubated with primary antibodies: rabbit polyclonal anti-SARS-CoV Spike at a dilution of 1:200 (40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse monoclonal anti-SARS-CoV NP at a dilution of 1:200 (40143-MM05 ...
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bioRxiv - Immunology 2021Quote: ... and blotted onto nitrocellulose membranes which were probed with the SARS-CoV2/2019-nCoV Spike rabbit polyclonal Ab (PAb, cat# 40591-T62, Sino Biologicals; dilution 1:1,000) followed by anti-Rabbit IgG-HRP conjugated antibody (1:15,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...