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Citations for Sino Biological :
801 - 842 of 842 citations for 2 1 1 Diethoxy 2 methyl propyl 4' Nitrophenyl Carbonate d6 since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: The recombinant forms of human ACE2 (residues 1-740) and RBD (residues 319-541) were purchased from Sino Biological (Wayne, PA). Both proteins were extensively dialyzed in 150 mM NH4CH3CO2 prior to MS analyses ...
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bioRxiv - Microbiology 2020Quote: ... was collected for 120 s prior to immersing the sensors in a 1:3 serial dilution of his-tagged human ACE2 (Sino Biologicals) ranging from 1,000 to 0.83nm in 10X Kinetics Buffer ...
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bioRxiv - Microbiology 2021Quote: ... and huCD4 (Met 1-Trp 390; catalog no. 10400-H08H) proteins with C-terminus 6x-His-tags were purchased from Sino Biological.
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... using a combination of polyclonal antibodies against the viral spike protein (1:200 dilution of anti S1 RBD cat. no. 40592-T62, and 1:200 dilution of anti S2 cat. no. 40590-T62, both from Sino Biological), for 3 h at room temperature ...
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bioRxiv - Cell Biology 2024Quote: ... IP complexes were sequentially eluted first with 50 ul of 1 mg/ml HA peptide (Sino Biological, catalog #PP100028, lot #IP15MA1401X) then boiling in sample buffer 95°C for 10 minutes.
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells were starved for 5 hours in DMEM/F12 supplemented with 1% PS and then stimulated with 100ng/mL BDNF (50240-MNAS, Sino Biological) for different time periods (5 ...
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bioRxiv - Immunology 2024Quote: ... improperly folded RBD variants in XBB.1.5 and JN.1 DMS libraries were removed using ACE2 (Sino Biological, 10108-H08H-B) conjugated biotin binder beads (Thermo Fisher ...
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bioRxiv - Cancer Biology 2020Quote: ... The resultant fluorescent PAA-nanopatterns of Fab anti-Fc antibody fragment protein were incubated overnight with 1 mg/mL ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (Gibco ...
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bioRxiv - Bioengineering 2022Quote: ... in 1 x TBST (Fisher AAJ77500K2) for 1 hour after the direct addition of 1.5 μl of either recombinant HER4 (Sino Biological 10363-H08H), or recombinant ATF-6 Beta (Abnova H00001388-Q01) ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 h at 37 °C ...
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bioRxiv - Bioengineering 2020Quote: ... Plates were washed with PBST four times and then incubated with 70 μL per well of 1:2,000 dilution of Anti-GFP antibody (Rabbit PAb, Sino Biological, #13105-RP01) for 1 hour at 37 °C ...
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bioRxiv - Biochemistry 2021Quote: ... HIV-1 Gag p24 or actin using anti-V5 (for pseudovirion detection; V2660)/anti-S2 (for virion detection; Sino Biologicals; 40590-T62), anti-N (Sino Biologicals ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were grown till 70-80% confluency and then treated for 48 hours with 1 μM recombinant mouse interferon gamma (Sino Biological, China). Treated cells were detached by trypsinisation and stained with antibodies against the MHC cell surface proteins.
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were grown as spheroids and media supplemented with 0.2μg/mL each Human Epidermal Growth Factor Protein (Sino Biological, 10605-HNAE-1) and Human Recombinant bFGF/FGF2 Protein (Sino Biological ...
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bioRxiv - Immunology 2022Quote: ... BA.2.12.1 (Acro SPN-C522d-50ug) and BA.4/5 (SPN-C5229-50ug) were purchased from Sino Biological and AcroBiosystems ...
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bioRxiv - Biochemistry 2020Quote: ... (Cat # 10108-H08H), and S1-His (spike protein residues 1-667, C-terminal His tag) (Cat # 40150-V08B1) were acquired from Sino Biological (Wayne, PA). All proteins were reconstituted in 1X PBS at pH 7.4 supplemented with 0.05 mg/mL bovine serum albumin (BSA) ...
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bioRxiv - Microbiology 2021Quote: ... Then the sections were incubated with primary antibodies: rabbit anti-SARS-CoV Spike (1:200, 40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse anti-SARS-CoV NP (1:200 ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 1:500 dilution ratio of primary antibody specific for SARS-CoV nucleoprotein56 (Rabbit mAb) (Sino Biological Inc. China), which cross-reacting with the NP protein of SARS-CoV–2 as well ...
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (pH 6.0) for 12 min for SARS-CoV-1 nucleoprotein antibody (Sino Biological Inc.; Beijing, China) and using recombinant protease from Streptomyces griseus (PanReac Applichem ...
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bioRxiv - Synthetic Biology 2022Quote: ... were coated overnight at 4 °C with 50 μL of 5-μg/mL in-house prepared ColE7 in PBS for ribosome-tethered Im7N58 or the same amount of commercial extracellular domain (residues 1 to 652) of human HER2 (HER2-ED; Sino Biological, 10004-HCCH) for ribosome-tethered scFv–HER2DQNAT in PBS ...
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bioRxiv - Immunology 2024Quote: ... The membranes were blocked and incubated with primary antibodies at 37℃ for 1 h to detect the S1 and S2 subunits using rabbit anti-SARS-Spike S1 (Sino Biological, 40591-T62) and rabbit anti-SARS-Spike S2 (Sino Biological ...
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bioRxiv - Immunology 2024Quote: ... again using mouse LYVE1 and additionally mouse LYVE-1 fused to human IgG1 Fc at the C-terminus (50065-M02H, Sino Biological, Beijing, China). To exclude potential nanobody clones binding to human IgG1 Fc ...
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bioRxiv - Immunology 2020Quote: ... Cells were fixed with 4% formaldehyde in PBS and stained with polyclonal rabbit anti-SARS-CoV Ab (Sino Biological) and secondary Alexa488 conjugated goat-anti-rabbit Ab (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... BA.2.12.1(Acro SPD-C522q-100ug) and BA.4/5(Acro SPD-C522r-100ug) were purchased from Sino Biological and AcroBiosystems ...
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bioRxiv - Microbiology 2020Quote: ... Then the sections were incubated with primary antibodies: rabbit polyclonal anti-SARS-CoV Spike at a dilution of 1:200 (40150-T62-COV2, Sino Biological, Chesterbrook, PA, USA), mouse monoclonal anti-SARS-CoV NP at a dilution of 1:200 (40143-MM05 ...
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bioRxiv - Immunology 2021Quote: ... and blotted onto nitrocellulose membranes which were probed with the SARS-CoV2/2019-nCoV Spike rabbit polyclonal Ab (PAb, cat# 40591-T62, Sino Biologicals; dilution 1:1,000) followed by anti-Rabbit IgG-HRP conjugated antibody (1:15,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...
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bioRxiv - Cell Biology 2023Quote: Cells were treated with either the recombinant gp120 of HIV-1 Group M Subunit A (Catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H ...
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bioRxiv - Immunology 2021Quote: ... ELISA (enzyme-linked immunosorbent assay) plates were coated overnight at 4°C with 1μg/ml of nucleoprotein or hemagglutinin (Sino Biological) diluted in PBS ...
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bioRxiv - Immunology 2022Quote: ... were coated overnight at 4°C with 2.5 μg/ml of the following recombinant proteins (in PBS) purchased from Sino Biological Inc ...
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bioRxiv - Cell Biology 2023Quote: ... These patterns were then coated overnight at 4°C with a recombinant E-cadherin Fc Tag protein (10204, Sino Biological) at a concentration of 20μg/mL ...
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bioRxiv - Microbiology 2020Quote: ... followed by rabbit monoclonal antibody against SARS-CoV2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Immunology 2022Quote: ... Mice serum was 3-fold diluted starting from 1:15 (Omicron) or 1:45 (WT) in the blocking buffer with a final amount of 50 ng biotinylated hACE2 (Sino Biological, cat# 10108-H08H-B) / 8 ng epitope 3 nanobody / 8 ng epitope 4 nanobody at each concentration and then incubated with the plate at room temperature for 2 hrs ...
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bioRxiv - Microbiology 2021Quote: ... the cultures were fixed with 4% PFA and wholemount labeled with anti-Spike antibody (Clone ID007, Cat. No. 40150-R007, Sino Biological) followed by Alexa-Dye conjugated secondary antibody ...
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bioRxiv - Immunology 2023Quote: ... and blocking activity of anti-CTLA-4 monoclonal antibodies were determined by ELISA using recombinant human B7.1 (Sino Biological, #10698-HCCH), human CTLA-4 (Sino Biological ...
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bioRxiv - Microbiology 2021Quote: ... we fixed the cells with 4% formaldehyde/phosphate-buffered saline (PBS) and stained the cells with polyclonal rabbit anti-SARS-CoV antibody (Sino Biological, https://www.sinobiological.com) and a secondary peroxidase-labeled goat anti-rabbit IgG (Dako ...
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bioRxiv - Microbiology 2023Quote: ... groups of five 8-to-12-week C57B6 mice (Taconic Farms Inc) were immunized with 4 μg/mouse of HCoV-OC43 S-His (Sino Biological # 40607-V08B) or N-His protein (Sino Biological # 40643-V07E ...
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bioRxiv - Immunology 2021Quote: 96-well microtiter plates were coated over night at 4°C with 100ng per well of recombinant NTD (Sino Biological; Beijing, China #40591-V49H), SARS-CoV-2 Spike S2 or S1 Glycoprotein (Virion\Serion ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% BSA in TBS containing 0.1% tween-20 (TBS-T) for 1h at 4 °C before incubating with anti-S2 Spike primary antibody (rabbit polyclonal, Sino Biologicals, Cat#: 40590-T62) at 1:1,000 dilution in TBS-T with 5% BSA for 14-16 h at 4°C ...
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bioRxiv - Bioengineering 2024Quote: Coating streptavidin-coated fluorescent Nile Red and Purple particles with biotinylated human IL-2Rβ (Acro Biosystems, ILB-H82E3) and human 4-1BB (Sino Biological, 10041-H27H-B) was performed following the same procedure as for the flow cytometry experiments ...