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Citations for Sino Biological :
701 - 750 of 785 citations for Glyphosate Chemical Purity 96% 2 13C 99%; 15N 98+% 100 Ug Ml In H2O since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 3.7% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) and secondary antibody anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked in 5% BSA in TBS + 0.1% Tween-20 (TBST) at room temperature before incubation at 4°C overnight with primary antibodies: SARS-CoV-2 spike RBD (Sino Biological, 40592-T62), GAPDH (BioLegend ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Cancer Biology 2023Quote: ... INHBA (Cat# 10429, -HNAH), and neuregulin-beta1 (Cat# 11609-HNCH, Ser 2-Lys 246 N-terminal fragment) were all from Sino Biological (Houston, TX). Human NRG1-specific siRNAs (5’-UCGGCUGCAGGUUCCAAAC ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) VWF-bound gcEVs (VWF+gcEVs) obtained by incubation of gcEVs with 10 µg of recombinant (r) human VWF (Sino Biological, Beijing, China); (3 ...
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bioRxiv - Bioengineering 2021Quote: ... Coating solution was prepared using sterile PBS combined with 15ug/mL Fc-tagged DLL4 (Sino Biological, 10171-H02H) and 2.5ug/mL Fc-tagged VCAM1 (R&D Systems ...
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bioRxiv - Microbiology 2021Quote: Bilayers were formed by the process described previously and incubated with 0.06 mg/ml GFP solution (Sino Biological) for 30 minutes at 30°C ...
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bioRxiv - Molecular Biology 2020Quote: ... YKL-40 group (HUVECs stimulated with 100ng/ml YKL-40, Cat No: 11227-H08H, Sino Biological Inc. China), YKL-40+lenti-synd4 shRNA group (HUVECs transfected with lentivirus encoding syndecan-4 shRNA plus 100ng/ml YKL-40 ...
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bioRxiv - Immunology 2020Quote: ... ELISA plates were also coated with 1 μg/mL recombinant SARS-CoV S1 protein (Sino Biological 40150-V08B1) and RBD protein (Sino Biological 40592-V08B ...
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bioRxiv - Molecular Biology 2022Quote: ... cells were incubated with human recombinant TGF-β1 (5 ng/ml, 10804-HNAC, Sino Biological Inc., Beijing, China), CMLD-2 (LN03203514 ...
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bioRxiv - Immunology 2021Quote: ... were fixed after 48 hrs with 1:1 solution of methanol/ acetone and air-dried prior to immunostaining with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62; Sino Biological; 1:1000 dilution) and HRP-conjugated anti-Rabbit IgG antibody (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% skim milk in PBS-T and incubated with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62, Sino Biological; 1:1,000 dilution) overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers ...
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bioRxiv - Microbiology 2022Quote: ... The Vero E6 cells were fixed and stained 20 hours (h) after infection and immunostained with a SARS-CoV/SARS-CoV-2 Nucleocapsid monoclonal antibody (Sino Biological, Wayne, PA, USA) followed by a horseradish peroxidase (HRP)-labeled Goat anti-Mouse IgG (cat # G-21040 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Molecular Biology 2020Quote: ... 1A10 were prepared by immunizing BALB/c mice with recombinant SARS-CoV-2 RBD fused with a C-terminal mouse IgGFc tag (Sino Biological Inc, Beijing, China) using previously described protocols (Qu et al. ...
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bioRxiv - Genetics 2020Quote: ... Loaded sensors were dipped into recombinant SARS-Cov-2 His-tagged Spike protein (D614 or D614G, Sino Biological, Cat # 40591-V08H and 40591-V08H3).
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Pathology 2020Quote: ... for 40 minutes, followed by a 60-minute incubation with the primary antibodies (SARS-CoV-2 nucleoprotein, mouse IgG1 (Sino Biological, cat#40143-MM08); ACE2 ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with an anti-SARS-CoV/SARS-COV-2 nucleocapsid (N) protein rabbit monoclonal antibody (#40143-R001, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at RT for 45 min ...
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bioRxiv - Microbiology 2020Quote: A full-length wild-type pCMV3-SARS-CoV-2 Spike (S1+S2)-long (termed as pCMV-S, codon-optimized, Sino Biological, cat # VG40589-UT) plasmid was used as a template to generate tagged pCMV-S ...
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bioRxiv - Immunology 2020Quote: ... RBD219-N1C1 was detected using a rabbit monoclonal antibody against the SARS-CoV-2 Spike S1 protein (Sino Biological, Beijing, China; Cat#: 40150-R007) and goat anti-rabbit IgG secondary antibodies conjugated with horseradish peroxidase (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed with 4% PFA and were incubated with either a polyclonal antibody against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or a monoclonal antibody against the SARS-CoV-2 S1 protein (MyBioSource ...
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bioRxiv - Immunology 2021Quote: ... Fractions which contain HexaPro protein were pooled and analyzed with SDS-PAGE and western blot against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or pooled convalescent sera ...
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bioRxiv - Immunology 2021Quote: ... The cells were then incubated with 200 ng/well of R-PE labelled rabbit anti-SARS-CoV-2 spike neutralizing monoclonal antibody (Sino Biological, Cat: 40592-R001) at 4°C for 2 h ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 antigen production in cells was detected by successive incubations with an anti-SARS-CoV nucleoprotein mouse monoclonal antibody (Sino Biological catalog# 40143-MM05), HRP IgG conjugate (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then fixed in 4% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171). Cells were then stained with anti-rabbit-IgG-FITC (Sigma ...
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bioRxiv - Bioengineering 2023Quote: ... The sample solutions were prepared by spiking biotinylated SARS-CoV-2 N protein rabbit monoclonal antibody (no. 40143-R004-B; Sino Biological, Inc.; Beijing, China) in buffer solution (0.1% BSA and 0.05% Tween 20 in PBS ...
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bioRxiv - Cell Biology 2024Quote: ... The expression vector for the SARS-CoV-2 Spike protein (pCMV3-2019-nCoV-Spike (S1+S2)-long) was purchased from Sino Biological (VG40589-UT, China). The expression vector for ACE2 was purchased from Miaolinbio (pEnCMV-ACE2 (human)-3×FLAG ...
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bioRxiv - Immunology 2021Quote: ... streptavidin beads were coated as described above with the following biotinylated antigens: 10 µg/mL MERS spike (Sino Biological, 40069-V08B ...
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bioRxiv - Immunology 2021Quote: ... One hundred μL of 20 μg/mL 2019-nCoV Spike Protein S1 (Fc Tag) (Sino Biological, CN: 40591-V02H) was mixed with 100 μL of antisera dilutions (serially 5-fold diluted from 1:5 to 1:3,125 ...
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bioRxiv - Immunology 2023Quote: ... A standard curve for calculations of unknowns was constructed using recombinant human TREM2 (4000–62.5 pg/mL, Sino Biological), and samples were diluted 1:4 before being assayed
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bioRxiv - Microbiology 2024Quote: ... the cells were incubated at an OD600 of 8/mL in FACS staining buffer (washing buffer + 0.5 mg/mL of Bovine Serum Albumin) with 9.09 nM hACE2-muFc (Sino Biological) for 1h ...
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bioRxiv - Biochemistry 2021Quote: ... consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain was purchased from Sino Biological (RBD-Fc, Cat#: 40592-V05H). For kinetic interaction analysis of RBD escape mutants and sybodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilised in methanol 0.6% H2O2 and stained for 1 h with an antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; 40143-R019, 1:300 dilution). Cells were further stained with the secondary antibody anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 10% FBS in 0.1% Tween-20 PBS (PBST) for 1 h prior to staining with a rabbit polyclonal anti SARS-CoV-2 nucleocapsid antibody (Sino Biological Inc. catalog # 40588-T62) diluted 1:500 and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the sections was incubated with corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:200; anti-SARS-CoV-2 Spike antibody, 40150-R007, Sino Biological, China, dilution 1:400) for 16 hours at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with the corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:2000; anti-SARS-CoV-2 Spike antibody, 40150-R007, Sino Biological, China, dilution 1:2000) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Chamber slides were washed twice with PBS-tween and then incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:1,000 in PBSK for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... followed by rabbit monoclonal antibody against SARS-CoV-2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Microbiology 2021Quote: ... For the detection of Spike protein-similar structures the samples were first incubated in 50 μL containing 0.5 μg SARS-CoV-2 Spike Neutralizing Antibody (clone: HA14JL2302, Sino Biological Inc. Cat. No: 40592-R001) or Neutralizing Antibody isolated from COVID-19 patients for 15 min at 4 °C then washed with PBS and stained again in 50 μL of the anti-Rabbit Alexa 647 (7,5μg/ml ...
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bioRxiv - Immunology 2020Quote: ... was quantitated then added at stoichiometric ratios (starting at 1:1) to 170 ng (750 fmol) of SARS-CoV-2 S1 trimer complex (Sino Biological, Cat: 40591-V08H; RU171) that had been dye-labeled with Dy-549P1 NHS ester in a total volume of 100 μL ...
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bioRxiv - Microbiology 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibody against the SARS-CoV-2 nucleocapsid protein (1:1000, Sino Biologicals US Inc, Wayne, PA). After washing 3 times with wash buffer ...
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bioRxiv - Immunology 2023Quote: ... Paraffin blocks containing the left lung lobe were sectioned at approximately 4 microns and stained using hematoxylin and eosin (H&E) or immunostained using a rabbit monoclonal antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; cat. no. 40588-R002) and rabbit polyclonal antibodies against CD-3 protein (Dako ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells were seeded in 60 mm plates then transfected with 2 µg of N-terminal tagged GFP-HMGB1 DNA plasmid DNA (Sino biological, cat no. HG10326-ANG). In brief ...
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bioRxiv - Immunology 2022Quote: ... were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... ELISA (enzyme-linked immunosorbent assay) plates were coated overnight at 4°C with 1μg/ml of nucleoprotein or hemagglutinin (Sino Biological) diluted in PBS ...
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bioRxiv - Biochemistry 2020Quote: ... using a GeSiM Nano-Plotter 2.1 at the following concentrations: 60 μg/mL for S1 (40591-V08H, Sino Biological Inc.) and 25 μg/mL for RBD (40592-V08H ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc.) for 30 min before transfection ...