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Citations for Sino Biological :
651 - 700 of 733 citations for Chikungunya Virus E2 Envelope Protein HEK293 Human Fc Tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... 96-well plates were coated with Spike 1 protein of SARS-CoV-2 (0.1μg/100 μL, Sino Biological, 40591-V08H), the tested sera were diluted at 1:100 and added to each well ...
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bioRxiv - Immunology 2022Quote: ... were coated with SARS-CoV-2 spike protein (SARS-CoV-2 S1+S2_ECD, 200 ng/well) (Sino Biological, Beijing, China) in PBS and incubated at 4 °C overnight ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Immunology 2022Quote: Recombinant His-tagged Spike S1 wild-type produced in baculovirus-insect cells and with < 1.0 EU per μg protein as determined by the LAL method (#40591-V08B1, Sino Biological) or flagellin (Invivogen ...
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bioRxiv - Neuroscience 2022Quote: ... DRG tissue lysates were immunoprecipitated over 2 hours at 4°C with protein A/G coupled with antibodies (rabbit anti-FcγRI 1:1000, Cat: 80016-R015, Sino Biological Inc. ...
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bioRxiv - Synthetic Biology 2022Quote: ... After bilayer formation beads were washed two more times with PBS and 5pmol of His-tagged proteins added (Sino Biological: ACE2-His 10108-H08H ...
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bioRxiv - Neuroscience 2023Quote: ... a rabbit polyclonal antibody against the SARS-CoV-2 nucleocapsid protein (dilution: 1:4,000, catalog number: 40143-T62, Sino Biological) was applied to the slides for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... The S1-specific binding assays were coated with 100 ng/well of the SARS-CoV-2 spike S1-His Recombinant Protein (Sino Biological) using high-binding 96-well plates (Santa Cruz Biotechnology) ...
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bioRxiv - Immunology 2022Quote: ... at 4 °C for 30 min and then stained with biotin-conjugated SARS-CoV-2 RBD protein (Sino Biological, 40592-V05H) at 4 °C for 20 min ...
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bioRxiv - Microbiology 2022Quote: ... were prepared for H&E staining and immunofluorescence staining for SARS-CoV-2 detection using the Coronavirus nucleocapsid protein (NP) antibody (cat. 40143-MM05, Sino Biological). Images were collected under a Pannoramic MIDI system (3DHISTECH ...
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bioRxiv - Immunology 2022Quote: ... recombinant SARS-CoV-2 Spike S1-HIS proteins (Sino Biological Europe for the Wild Type Wuhan, or Omicron from Sino Biological Europe and from Covalab ...
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bioRxiv - Immunology 2022Quote: ... containing 10 μg OVA or 5 μg of the spike protein of SARS-CoV-2 (the receptor-binding domain; recombinant product from Sino Biological) mixed with or without 10 μg or 20 μg S100A4 as indicated ...
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bioRxiv - Immunology 2021Quote: SARS-CoV-2 wild-type (WT) and mutated (Beta: K417N-E484K-N501Y, Delta: L452R-T478K, Lambda: L452Q-F490S) RBD proteins (Sino Biological) were separately coated into 96-well plates at 4 °C overnight ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The L19-mTNF fusion protein was expressed by transient gene expression in CHO-S cells and purified from the cell culture supernatant to homogeneity by protein A (Sino Biological) chromatography ...
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bioRxiv - Biochemistry 2021Quote: ... The recombinant SARS-CoV-2 spike regional-binding domain protein expressed in the HEK 293 cell line was purchased from Sino Biological Inc ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with either 2 ug/ml SARS-CoV-2 Spike Protein ECD (Sino Biological, 40589-V08B1) or 1 ug/ml SARS-CoV-2 Spike-RBD (Sino Biological ...
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bioRxiv - Cancer Biology 2020Quote: ... the fusion protein was produced in CHO-S mammalian cells and purified from the cell culture medium by affinity chromatography using a Protein A (Sino Biological) affinity column ...
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bioRxiv - Microbiology 2021Quote: ... of 20 patients with COVID-19 obtained in February and October 2020 in Chongqing were previously reported.2 Eight RBD-specific mAbs with neutralizing capability against SARS-CoV-2 were obtained from the blood samples of COVID-19 convalescent patients.4 DNA sequences encoding reference strain (wild-type, WT) and mutant Spike proteins of SARS-CoV-2 were codon-optimized and synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding reference strain (GenBank: QHD43416) SARS-CoV-2 Spike protein with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: The codon-optimized gene encoding SARS-CoV-2 S protein (GenBank: QHD43416) with C-terminal 19-amino acid deletion was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Microbiology 2020Quote: ... The rabbit polyclonal antibody against SARS-CoV-2 SP (Cat# 40150-R007) and ACE2 protein (Cat# 40592-T62) were obtained from Sino Biological and anti-HIVp24 monoclonal antibody was described previously [35,36] ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... The cells were formalin-fixed and ethanol permeabilized followed by incubation with a murine monoclonal antibody which targets the viral nucleocapsid protein (Sino Biological), followed by a secondary anti-mouse IgG peroxidase conjugate (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... were coated overnight with 1 ug/ml SARS-CoV-2 (2019-nCoV) Spike Receptor Binding Domain (polyhistidine-tagged) recombinant protein (Sino Biological) diluted in PBS ...
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bioRxiv - Immunology 2022Quote: ... RBD protein expression was analyzed using a rabbit polyclonal antibody SARS-CoV-2 Spike RBD Antibody (HRP) (Sino Biological, #40592-T62), which cross-reacts with SARS-CoV RBD protein ...
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bioRxiv - Microbiology 2021Quote: ... were washed and coated with 50 μl per well of 2μg/ml of recombinant spike protein (Sino Biological; 40589-V08B1-B), diluted in 0.1% BSA ...
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bioRxiv - Microbiology 2020Quote: ... specific S protein levels were identified using specific primary antibodies (NB100-56578, Novus Biologicals and 40150-T62-CoV2, Sino Biological Inc.), and HRP conjugated secondary antibody (Protein Simple) ...
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bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
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bioRxiv - Immunology 2021Quote: ... Presence of virus replication in each well was assessed by visual examination of cytopathic effect along with antibody staining for N protein antigen (mouse monoclonal antibody 40143-MM08, Sino Biological) with fluorescent secondary antibody detection ...
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bioRxiv - Microbiology 2021Quote: ... Cells were treated with methanol 0.6% H2O2 and stained for 1 h with a 1:3000 dilution of 40143-R019 rabbit mAb to SARS-CoV-2 nucleocapsid protein (Sino Biological). A 1:3000 dilution of sheep anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Immunology 2020Quote: The following proteins were coated on 96 well ELISA plates at the following concentrations: SARS-CoV RBD (Sino Biological, 40150-V08B1) at 1 µg/ml ...
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bioRxiv - Cell Biology 2020Quote: Caco-2 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV-2 nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological), followed by an HRP-labeled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Western Blot detection of SARS-CoV-2 Spike protein was performed using a polyclonal Spike antibody (Sino Biological Cat. # 40589-V08B1).
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bioRxiv - Immunology 2021Quote: ... All vaccinations were administered intranasally to anesthetized mice in 50 ul saline with 10 ug NP (Influenza A H1N1 Nucleoprotein / NP Protein, Sino Biological) or 20ug DQ-OVA protein (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Spike S1-His Recombinant Protein (40591-V08B1) and SARS-CoV-2 (2019-nCoV) Spike S2 ECD-His Recombinant Protein (40590-V08B) were obtained from Sino Biological. SARS-CoV-2 Spike protein (RBD ...
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bioRxiv - Microbiology 2021Quote: The codon-optimized gene encoding SARS-CoV-2 spike (S) protein (GenBank: QHD43416) with 19 amino acids deletion at the C-terminal was synthesized by Sino Biological Inc (Beijing ...
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bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Microbiology 2022Quote: ... The copies of the phage-packaged NP protein were quantified by Western blotting using the commercial rabbit anti-NP antibody (Sino Biological) and NP protein standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of Expi293™ cells transfected with pAd/SARS-CoV-2-S1SA was diluted 1:40 in PBS-T with 1% BSA and along with standard control protein 40591-V08H (rS1H, Sino Biological) or purified rSARS-CoV-2S1Beta were incubated overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Various dilutions of SEC-purified S-EABR eVLP samples and known amounts of soluble SARS-CoV-2 S1 protein (Sino Biological) were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... IHC was carried out using a rabbit monoclonal antibody for SARS-CoV nucleocapsid protein (catalogue #40143-R001, clone #001, Sino Biological) which cross-reacts with SARS-CoV-2 nucleocapsid protein ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Immunology 2024Quote: ... were coated with 100 ng/well of SARS-CoV-2 (2019-nCOV) Spike S1 + S2 ECD-His-Recombinant Protein (40589-V08B1, Sino Biological) overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... the “BiEV index (caspase 14)” was used based on the fluorescein-labeled recombinant caspase 14 protein (11856-H07E; Sino Biological, Beijing, China) labeled with NHS-fluorescein (Thermo Fisher Scientific ...
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Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: ... then proteins were monitored with ECL Western blotting substrate (Pierce) after incubation with anti-SARS-CoV-2-S-RBD (Sino Biological, China) or anti-β-actin antibody (Solarbio ...