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Citations for Sino Biological :
651 - 692 of 692 citations for 2 Trimethylsilyl Furo 3 2 B Pyridine 6 Carbaldehyde since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Bioengineering 2021Quote: ... washed five times with PBST and incubated at room temperature for 1 h with 100 µL of 1 µg/mL anti-SARS-CoV-2 spike rabbit polyclonal antibody (Sino Biological, Beijing, China, Cat#40589-T62) in PBSCT ...
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bioRxiv - Immunology 2021Quote: ... the cells were permeabilized with Triton X-100 and probed with a SARS-CoV/SARS-CoV-2 nucleoprotein-specific rabbit primary antibody (Sino Biological, Wayne, PA, USA, 40143-R001) followed by an Alexa Fluor 647-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... and stained with Coomassie Blue or transferred to a polyvinylidene difluoride membrane and probed with a monoclonal anti-SARS-CoV-2 Spike rabbit antibody recognizing the RBD region (Sino Biological, Beijing, China; Cat # 40150-R007) to evaluate the size and confirm the identity ...
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bioRxiv - Microbiology 2021Quote: ... 96-well plates were coated overnight with either 1 μg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag, Sino biological, Cat. No. 40592-V08B-100) or recombinant SARS-CoV-2 Nucleocapsid His Protein ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 His-tagged Spike (S) protein and the receptor binding domain (RBD) protein of S were purchased from Sino Biological (Beijing/China and Wayne/USA).
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bioRxiv - Bioengineering 2023Quote: Nectagen’s nanoCLAMP phage display library NL-21 was panned as described (Suderman et al. 2017) against SARS CoV 2 Spike protein RBD (Sino Biologicals, S1RBD-Fc, cat#40592-VO2H) for rounds 1 and 2 ...
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bioRxiv - Microbiology 2021Quote: ... the proportion of transfected cells was determined by staining with biotinylated RBD (Sino biologicals, Cat: 40592-V08H-B) for 30 min ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked with 5% milk in PBST before the membrane was incubated with a primary antibody (rabbit anti-SARS-CoV-2 S2 polyclonal antibody, Sino Biological, Cat# 40590-T62, 1:2500 dilution) in PBST ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated with a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV2-S1 (Sino Biological, 40591-V08H, n=3) and SARS-CoV2-S2 (Sino Biological ...
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bioRxiv - Bioengineering 2023Quote: Binding of IL-3 (Sino Biological, PN: 11858-H08H) (analyte in solution ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human recombinant Galectin 3 (His tag) (Sino Biological Inc.) in PBS was then applied to the wells coated with svPDE after the wells were washed with PBST three times ...
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bioRxiv - Immunology 2023Quote: Recombinant SARS-CoV-2 Spike RBD proteins from WT (#40592-V08H) and Omicron (B.1.1.529; #40592-V08H121) strains were purchased from Sino Biological and labelled with AlexaFluor 647 as previously described(25) ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV2-S2 (Sino Biological, 40590-V08B, n=3), respectively ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...
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bioRxiv - Biochemistry 2022Quote: ... that had been preincubated for 30 minutes with 12.5 μL of Biotinylated-ACE2 (Sino Biological, Cat. No. 10108-H27B-B). After a 30 minute incubation at 37 °C ...
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bioRxiv - Microbiology 2021Quote: ... were washed and coated with 50 μl per well of 2μg/ml of recombinant spike protein (Sino Biological; 40589-V08B1-B), diluted in 0.1% BSA ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2023Quote: ... RBD (Cat. No. 40592-VNAH) and Spike S1 B.1.1.7 variant (Cat. no. 40591-V08H12) were acquired from Sino Biological (Beijing, China) and biotinylated using EZ-Link™ Sulfo-NHS-Biotin kit (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... improperly folded RBD variants in XBB.1.5 and JN.1 DMS libraries were removed using ACE2 (Sino Biological, 10108-H08H-B) conjugated biotin binder beads (Thermo Fisher ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was added to an immune plate (430341, Thermo Fischer Scientific, USA) coated with a monoclonal IL-12-B antibody (51004-R020, Sino Biological, China), and the plate was incubated at 4 °C for 18 h ...
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bioRxiv - Bioengineering 2023Quote: ... The 5A12 library was first incubated with either 0.5 nM VEGF (Acro, VE5-H82Q0-200ug) or 12 nM Ang2 (Sino Biological, 10691-H08S-B) plus Streptavidin-AlexaFluor647 (Bioloegend ...
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bioRxiv - Immunology 2023Quote: ... plates were incubated for 1 hour with serially diluted IgGs with 1µg/mL of human CTLA-4-Biotin (Sino Biological, #11159-H08H-B) in 1xPBS with 2% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... astrocyte cultures were treated with 3 ng/ml IL-1α (Sino Biological, 50114MNAE), 30 ng/ml TNF-α (PeproTech ...
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bioRxiv - Bioengineering 2024Quote: Coating streptavidin-coated fluorescent Nile Red and Purple particles with biotinylated human IL-2Rβ (Acro Biosystems, ILB-H82E3) and human 4-1BB (Sino Biological, 10041-H27H-B) was performed following the same procedure as for the flow cytometry experiments ...
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bioRxiv - Immunology 2022Quote: ... Mice serum was 3-fold diluted starting from 1:15 (Omicron) or 1:45 (WT) in the blocking buffer with a final amount of 50 ng biotinylated hACE2 (Sino Biological, cat# 10108-H08H-B) / 8 ng epitope 3 nanobody / 8 ng epitope 4 nanobody at each concentration and then incubated with the plate at room temperature for 2 hrs ...
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bioRxiv - Biophysics 2020Quote: ... when the cells were grown to a density of 2.0×10 6/ml in SMM 293T-I medium (Sino Biological Inc.) supplemented with 1% FBS under 5% CO2 in a shaker at 130 rpm at 37°C ...
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bioRxiv - Bioengineering 2020Quote: ... Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological ...
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001 ...
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bioRxiv - Microbiology 2021Quote: 293F cells at the density of 6 × 105 cells/mL were seeded into 100 mL SMM 293-TII serum-free medium (Sino Biological, Cat. No. M293TII) one day before transfection ...
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bioRxiv - Microbiology 2023Quote: 293F cells at 6 × 105 cells/mL density were seeded into 100 mL SMM 293-TII serum-free medium (Sino Biological, Cat. No. M293TII) one day before transfection ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc.) for 30 min before transfection ...
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bioRxiv - Microbiology 2020Quote: ... was collected for 120 s prior to immersing the sensors in a 1:3 serial dilution of his-tagged human ACE2 (Sino Biologicals) ranging from 1,000 to 0.83nm in 10X Kinetics Buffer ...
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bioRxiv - Microbiology 2021Quote: ... Plates were washed a further 3 times with 300 μL PBSB prior to the addition of 100 ng/mL BiotinylatedACE2 (Sino Biological) in PBSB + 1% casein (50 μL/ well ...
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bioRxiv - Cancer Biology 2021Quote: ... ID8 and SK-OV-3 cells were stimulated with 2.5 ng/mL murine and human recombinant IL-20 protein (Sino Biological, Beijing, China) for 24 hours ...