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Citations for Sino Biological :
601 - 646 of 646 citations for Vitamin K epoxide reductase complex subunit 1 VKORC1 Antibody Biotin since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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bioRxiv - Immunology 2022Quote: ... were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological) for 1 hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated for 1 hour at 4°C with 1μg/ml of anti-nucleoprotein mAb (40143-MM05; Sino Biological) diluted in permeabilization flow cytometry buffer (PBS supplemented with 5% FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... 0777)/1 X DPBS % v/v solution (DPBST) and 50 µl/well of 0.05 µg/ml H3 (Sino Biological; 11231-RP02) or 0.5 µg/ml H3K4me3 (Millipore ...
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bioRxiv - Pathology 2020Quote: ... Primary antibodies were incubated overnight at 4 °C in DPBS with 1% normal goat serum and 0.1% Tween-20 (rabbit anti-2019-nCoV NP [Sino Biological #40143-R019 ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 and SARS-CoV were stained with mouse-anti-SARS-CoV nucleoprotein (40143-MM05, 1:400, Sino Biological) or rabbit-anti-SARS-CoV nucleoprotein (40143-T62 ...
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bioRxiv - Microbiology 2020Quote: ... 96-well plates were coated with Spike 1 protein of SARS-CoV-2 (0.1μg/100 μL, Sino Biological, 40591-V08H), the tested sera were diluted at 1:100 and added to each well ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (1×106) were stimulated with SARS-CoV-2 spike protein (S1+S2_ECD, 5 μg/ml, Sino Biological, Beijing, China) or BSA (5 μg/ml ...
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bioRxiv - Synthetic Biology 2022Quote: ... were used to immobilize the peptide hormone and either Protein A (Fc tagged GLP-1 receptor, Sino Biological, 13944-H02H) or Ni-NTA (6x His tagged GH ...
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bioRxiv - Genomics 2024Quote: The human codon-optimized full-length SARS-CoV-2 spike (Wuhan-Hu-1 strain) gene was obtained from Sino Biological Inc ...
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bioRxiv - Cell Biology 2024Quote: 100x SYPRO Orange Protein Gel Stain (SigmaAldrich, S5692) was mixed with 1 µM purified recombinant PEBP1(Cat.No. 14582-HNAE, Sino Biological), and aliquoted it into each well of the 96-well plate in 11.5 µl total volume ...
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bioRxiv - Biochemistry 2020Quote: The recombinant forms of human ACE2 (residues 1-740) and RBD (residues 319-541) were purchased from Sino Biological (Wayne, PA). Both proteins were extensively dialyzed in 150 mM NH4CH3CO2 prior to MS analyses ...
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bioRxiv - Microbiology 2020Quote: ... was collected for 120 s prior to immersing the sensors in a 1:3 serial dilution of his-tagged human ACE2 (Sino Biologicals) ranging from 1,000 to 0.83nm in 10X Kinetics Buffer ...
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bioRxiv - Microbiology 2021Quote: ... were coated overnight with 1 ug/ml SARS-CoV-2 (2019-nCoV) Spike Receptor Binding Domain (polyhistidine-tagged) recombinant protein (Sino Biological) diluted in PBS ...
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bioRxiv - Immunology 2021Quote: ELISA plates were coated with RBD (SARS-CoV-2 WT, SARS-CoV-2 Omicron, SARS-CoV-1 RBD, Sino Biological Inc.) at 0.03 μg/mL and 1 μg/mL in PBS at 4°C overnight ...
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bioRxiv - Microbiology 2021Quote: ELISA plates (Evergreen Scientific) were coated with 100 μl per well of 1 μg/ml of SARS-CoV-2 S-ecto protein (Sino Biological), SARS-CoV-2 RBD-untagged protein (Sino Biological) ...
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bioRxiv - Immunology 2022Quote: ... The Omicron BA.1 RBD and BA.2 RBD used in ELISA were purchased from Sino Biological (Cat. No. 40592-V08H121) and AcroBiosystems (Cat ...
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bioRxiv - Microbiology 2021Quote: ... and huCD4 (Met 1-Trp 390; catalog no. 10400-H08H) proteins with C-terminus 6x-His-tags were purchased from Sino Biological.
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... Samples comprised of a 1.4:1 molar ratio of mAbs (10 μg/mL) to mouse Fc-RBD (2.5 μg/mL, Sino Biological, 51.5 kDa) were complexed in kinetics buffer for 30 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
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bioRxiv - Immunology 2022Quote: ... Plates were coated with 1 µg/ml of SARS-CoV-2 (2019-nCoV) spike RBD-His (Sino Biologicals, Cat. 40592-V08B) for 4-5h at 37°C and then blocked overnight in 1% BSA ...
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bioRxiv - Cell Biology 2024Quote: ... IP complexes were sequentially eluted first with 50 ul of 1 mg/ml HA peptide (Sino Biological, catalog #PP100028, lot #IP15MA1401X) then boiling in sample buffer 95°C for 10 minutes.
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bioRxiv - Immunology 2023Quote: ELISA assays were conducted by pre-coating ELISA plates with RBD (SARS-CoV-2 wild type, SARS-CoV-2 BA.1, SARS-CoV-2 BA.2 RBD, Sino Biological) at concentrations of 0.03 μg ml−1 and 1 μg ml−1 in PBS overnight at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... using a combination of polyclonal antibodies against the viral spike protein (1:200 dilution of anti S1 RBD cat. no. 40592-T62, and 1:200 dilution of anti S2 cat. no. 40590-T62, both from Sino Biological), for 3 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: SH-SY5Y cells were starved for 5 hours in DMEM/F12 supplemented with 1% PS and then stimulated with 100ng/mL BDNF (50240-MNAS, Sino Biological) for different time periods (5 ...
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bioRxiv - Immunology 2024Quote: ... improperly folded RBD variants in XBB.1.5 and JN.1 DMS libraries were removed using ACE2 (Sino Biological, 10108-H08H-B) conjugated biotin binder beads (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Bioengineering 2022Quote: ... in 1 x TBST (Fisher AAJ77500K2) for 1 hour after the direct addition of 1.5 μl of either recombinant HER4 (Sino Biological 10363-H08H), or recombinant ATF-6 Beta (Abnova H00001388-Q01) ...
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bioRxiv - Biochemistry 2021Quote: ... HIV-1 Gag p24 or actin using anti-V5 (for pseudovirion detection; V2660)/anti-S2 (for virion detection; Sino Biologicals; 40590-T62), anti-N (Sino Biologicals ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-1 pseudotyped virus neutralization assay) or 293T cells overexpressing human angiotensin-converting enzyme 2 (293T-hACE2) (Sino Biological Company) (for Pangolin-GD and RaTG13 pseudotyped virus neutralization assay) ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were grown till 70-80% confluency and then treated for 48 hours with 1 μM recombinant mouse interferon gamma (Sino Biological, China). Treated cells were detached by trypsinisation and stained with antibodies against the MHC cell surface proteins.
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bioRxiv - Bioengineering 2023Quote: ... The libraries were subjected to five rounds of biopanning against the recombinant SARS-CoV-2 BA.1 RBD protein (Sino Biological Inc.) as described previously38 ...
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bioRxiv - Cancer Biology 2024Quote: ... All cell lines were grown as spheroids and media supplemented with 0.2μg/mL each Human Epidermal Growth Factor Protein (Sino Biological, 10605-HNAE-1) and Human Recombinant bFGF/FGF2 Protein (Sino Biological ...
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bioRxiv - Biochemistry 2020Quote: ... (Cat # 10108-H08H), and S1-His (spike protein residues 1-667, C-terminal His tag) (Cat # 40150-V08B1) were acquired from Sino Biological (Wayne, PA). All proteins were reconstituted in 1X PBS at pH 7.4 supplemented with 0.05 mg/mL bovine serum albumin (BSA) ...
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bioRxiv - Immunology 2020Quote: ... were coated with SARS-CoV-1 RBD-rCD4-His or SARS-CoV-2 RBD-rCD4-His or MERS-CoV S1-His (Sino Biological, 40069-V08H), at 3 μg/mL ...
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bioRxiv - Immunology 2021Quote: ... 1μg of formalin and heat inactivated SARS-CoV 2 adjuvanted in Alhydrogel (Brenntag) 1% or 1.5 μg of recombinant SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1) adjuvanted in Alhydrogel (Brenntag ...
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bioRxiv - Synthetic Biology 2022Quote: ... were coated overnight at 4 °C with 50 μL of 5-μg/mL in-house prepared ColE7 in PBS for ribosome-tethered Im7N58 or the same amount of commercial extracellular domain (residues 1 to 652) of human HER2 (HER2-ED; Sino Biological, 10004-HCCH) for ribosome-tethered scFv–HER2DQNAT in PBS ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Immunology 2024Quote: ... again using mouse LYVE1 and additionally mouse LYVE-1 fused to human IgG1 Fc at the C-terminus (50065-M02H, Sino Biological, Beijing, China). To exclude potential nanobody clones binding to human IgG1 Fc ...
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bioRxiv - Immunology 2021Quote: ... and blotted onto nitrocellulose membranes which were probed with the SARS-CoV2/2019-nCoV Spike rabbit polyclonal Ab (PAb, cat# 40591-T62, Sino Biologicals; dilution 1:1,000) followed by anti-Rabbit IgG-HRP conjugated antibody (1:15,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... VSV-SARS-1-GFP single cycle particles were produced in Expi293 cells through transfection per manufacturer’s instructions with the SARS-CoV-1 spike expression plasmid (Sino Biological, Cat #VG40150-G-N) containing a 19-aa deletion in the cytoplasmic tail ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were plated in 6-well plates (2×105 cells/well) and treated with 1 ng/mL LPS and 20 ng/mL mIFNγ (Sino Biological Inc., Beijing, China) for 24 h ...
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bioRxiv - Immunology 2022Quote: ... Mice serum was 3-fold diluted starting from 1:15 (Omicron) or 1:45 (WT) in the blocking buffer with a final amount of 50 ng biotinylated hACE2 (Sino Biological, cat# 10108-H08H-B) / 8 ng epitope 3 nanobody / 8 ng epitope 4 nanobody at each concentration and then incubated with the plate at room temperature for 2 hrs ...
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Microbiology 2021Quote: ... 96-well plates were coated overnight with either 1 μg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag, Sino biological, Cat. No. 40592-V08B-100) or recombinant SARS-CoV-2 Nucleocapsid His Protein ...