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Citations for Sino Biological :
601 - 650 of 1040 citations for SARS CoV 2 Spike Glycoprotein S2 His Tag Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with C terminal OFP Spark fluorescent tag (pCMV3-C-OFPSpark) was obtained from Sino biological (#VG40591-ACR). Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A ...
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bioRxiv - Immunology 2021Quote: ... were fixed after 48 hrs with 1:1 solution of methanol/ acetone and air-dried prior to immunostaining with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62; Sino Biological; 1:1000 dilution) and HRP-conjugated anti-Rabbit IgG antibody (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% skim milk in PBS-T and incubated with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62, Sino Biological; 1:1,000 dilution) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Pathology 2020Quote: ... for 40 minutes, followed by a 60-minute incubation with the primary antibodies (SARS-CoV-2 nucleoprotein, mouse IgG1 (Sino Biological, cat#40143-MM08); ACE2 ...
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bioRxiv - Immunology 2021Quote: ... Cells were fixed with 4% PFA and were incubated with either a polyclonal antibody against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or a monoclonal antibody against the SARS-CoV-2 S1 protein (MyBioSource ...
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bioRxiv - Immunology 2021Quote: ... Fractions which contain HexaPro protein were pooled and analyzed with SDS-PAGE and western blot against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or pooled convalescent sera ...
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bioRxiv - Bioengineering 2023Quote: ... The sample solutions were prepared by spiking biotinylated SARS-CoV-2 N protein rabbit monoclonal antibody (no. 40143-R004-B; Sino Biological, Inc.; Beijing, China) in buffer solution (0.1% BSA and 0.05% Tween 20 in PBS ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-SARS-CoV NP at a dilution of 1:200 (40143-MM05, Sino Biological), mouse monoclonal anti-pan cytokeratin at a dilution of 1:100 (sc-8018 ...
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bioRxiv - Microbiology 2020Quote: The mouse mAb against the SARS-CoV nucleoprotein NP (40143-MM05) was purchased from Sino biologicals and used at dilutions of 1:500 for flow cytometry analysis and 1:1,000 for titration in TCID50 assays ...
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bioRxiv - Immunology 2022Quote: ... Anti-SARS-CoV-1 NP mouse monoclonal antibody (Sino Biological Inc.; Beijing, China, dilution: 1:500) and rabbit anti hamster IgA antibody (Brookwood Biomedical ...
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bioRxiv - Immunology 2020Quote: ... were coated overnight with 2μg/ml of SARS-CoV S or RBD proteins (Sino Biological, China). Serum samples were 2-fold serially diluted and S or RBD binding antibody (S-BAb or RBD-BAb ...
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bioRxiv - Microbiology 2020Quote: ... at dilution 1:500 and mouse monoclonal antibody against SARS-CoV NP (Sino Biological, 40143-MM05) at a dilution 1:500 for 2 h at room temperature ...
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bioRxiv - Immunology 2020Quote: ... and then human ACE2 recombinant protein (0.25 μg/ml, His tag; Sino Biological) was added to the wells and incubated for 2 h at RT ...
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bioRxiv - Immunology 2020Quote: ... luciferase reporter vector and a plasmid encoding for the wild type SARS-CoV2 Spike protein (Sino Biological) or vesicular stomatitis virus G (VSV-G ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Biochemistry 2021Quote: ... consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain was purchased from Sino Biological (RBD-Fc, Cat#: 40592-V05H). For kinetic interaction analysis of RBD escape mutants and sybodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilised in methanol 0.6% H2O2 and stained for 1 h with an antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; 40143-R019, 1:300 dilution). Cells were further stained with the secondary antibody anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 10% FBS in 0.1% Tween-20 PBS (PBST) for 1 h prior to staining with a rabbit polyclonal anti SARS-CoV-2 nucleocapsid antibody (Sino Biological Inc. catalog # 40588-T62) diluted 1:500 and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... followed by rabbit monoclonal antibody against SARS-CoV-2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Immunology 2020Quote: ... was quantitated then added at stoichiometric ratios (starting at 1:1) to 170 ng (750 fmol) of SARS-CoV-2 S1 trimer complex (Sino Biological, Cat: 40591-V08H; RU171) that had been dye-labeled with Dy-549P1 NHS ester in a total volume of 100 μL ...
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bioRxiv - Microbiology 2023Quote: ... Tissue sections were incubated overnight at 4°C with primary antibody against the SARS-CoV-2 nucleocapsid protein (1:1000, Sino Biologicals US Inc, Wayne, PA). After washing 3 times with wash buffer ...
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bioRxiv - Immunology 2023Quote: ... Paraffin blocks containing the left lung lobe were sectioned at approximately 4 microns and stained using hematoxylin and eosin (H&E) or immunostained using a rabbit monoclonal antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; cat. no. 40588-R002) and rabbit polyclonal antibodies against CD-3 protein (Dako ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated with rabbit polyclonal antibody against SARS-CoV S (Sino Biological, #40150-T62-COV2) at dilution 1:500 and mouse monoclonal antibody against SARS-CoV NP (Sino Biological ...
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bioRxiv - Microbiology 2023Quote: ... the plates were sequentially stained with cross-reactive rabbit anti-SARS-CoV-N IgG (Sino Biological Inc) as the primary antibody and HRP-conjugated goat anti-rabbit IgG (H + L ...
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bioRxiv - Microbiology 2020Quote: ... the cells were incubated with 1:500 dilution ratio of primary antibody specific for SARS-CoV nucleoprotein56 (Rabbit mAb) (Sino Biological Inc. China), which cross-reacting with the NP protein of SARS-CoV–2 as well ...
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bioRxiv - Microbiology 2021Quote: Full-length coronavirus S protein with His tag (500 ng) (40589-V08B1, Sino Biological), coronavirus S protein S1 subunit with His tag (500 ng ...
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bioRxiv - Microbiology 2021Quote: ... coronavirus S protein S1 subunit with His tag (500 ng) (40591-V08B1, Sino Biological), coronavirus S protein S2 subunit with His tag (500 ng ...
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bioRxiv - Microbiology 2020Quote: ... Positive control for the nucleocapsid ELISA was SARS-CoV nucleoprotein rabbit monoclonal antibody (Sino Biological Inc, Beijing, China). Negative controls were reagent grade human sera (compared to Mab CR3022) ...
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bioRxiv - Immunology 2020Quote: ... ELISA plates were also coated with 1 μg/mL recombinant SARS-CoV S1 protein (Sino Biological 40150-V08B1) and RBD protein (Sino Biological 40592-V08B ...
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bioRxiv - Molecular Biology 2024Quote: ... and MERS-CoV S2 subdomains as well as recombinant HCoV-NL63 and HCoV-229E S were purchased from Sino Biological.
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bioRxiv - Microbiology 2021Quote: ... and coronavirus S protein RBD with His tag (500 ng) (40592-V08B-B, Sino Biological) were used as coating reagents in 96-well plates (3361 ...
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bioRxiv - Immunology 2020Quote: ... S protein was probed with anti-SARS-CoV-1 S1 subunit rabbit polyclonal antibody (40150-T62-COV2, Sino Biological); N protein was probed with anti-SARS-CoV1 NP rabbit polyclonal antibody (40413-T62 ...
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bioRxiv - Immunology 2021Quote: ... sections were covered with a mouse monoclonal anti-SARS-CoV nucleocapsid protein (#40143-MM05, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:4,000 and incubated at room temperature for 45 min ...
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bioRxiv - Immunology 2021Quote: ... MERS-CoV Nucleoprotein protein (40068-V08B) and SARS Coronavirus Nucleocapsid Protein (40143-V08B) were from Sino biological (Beijing, China). Mammalian expression plasmid for SARS-CoV-2 nucleocapsid protein (152536 ...
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bioRxiv - Microbiology 2020Quote: ... polyclonal antibodies against SARS-CoV RP01 (Cat: 40150-RP01) and T52 (Cat: 40150-T52) were purchased from Sino Biological. Fetal bovine serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells are fixed with paraformaldehyde and stained by anti-SARS-2 nucleoprotein monoclonal antibody (Sino Biological) followed by peroxidase-conjugated goat anti-mouse IgG (SeraCare) ...
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bioRxiv - Immunology 2020Quote: ... Human IL1RL1/ST2 Protein (isoform a, His Tag) (Cat. #13034-H08H) was purchased from Sino Biological. NF-κB inhibitor Bay 11-7802 and JAK inhibitor 1 were both from EMD Millipore ...
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bioRxiv - Bioengineering 2020Quote: ... were coated with 2 mg/mL coronavirus spike proteins (the spike proteins and their variants are purchased from Sino biological) at least 2 hours at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were incubated overnight at 4°C with a primary antibody for SARS-CoV-2 (2019-nCoV) S protein (Sino Biological, 40589-T62; diluted 1:2,000 in PBS-T). Next ...
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bioRxiv - Immunology 2021Quote: ... One hundred μL of 20 μg/mL 2019-nCoV Spike Protein S1 (Fc Tag) (Sino Biological, CN: 40591-V02H) was mixed with 100 μL of antisera dilutions (serially 5-fold diluted from 1:5 to 1:3,125 ...
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bioRxiv - Molecular Biology 2021Quote: ... permeabilized with 70% ethanol and stained with polyclonal rabbit anti-SARS-CoV nucleoprotein antibody (1:1000; 40588-T62, Sino Biological) followed by secondary Alexa488 conjugated goat-anti-rabbit antibody (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: ... and incubated at room temperature on ExoView R100 (NanoView Biosciences, Brighton, MA) custom virus chips printed with SARS-CoV2-Spike antibodies (clones D001, D003, MM43, Sino Biological), anti-LPS (1E6 ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Human KRAS (G12D) and the corresponding His tag (12259-H07E1) were purchased from Sino Biological (Beijing, China). Human KRAS (G12V) ...
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bioRxiv - Biochemistry 2021Quote: ... Sf21 insect cells grown in SIM SF medium (Sino Biological Inc.) were used to generate and amplify the baculoviruses ...
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bioRxiv - Microbiology 2023Quote: ... The membrane was blocked with 5% BSA in TBS containing 0.1% tween-20 (TBS-T) for 1h at 4 °C before incubating with anti-S2 Spike primary antibody (rabbit polyclonal, Sino Biologicals, Cat#: 40590-T62) at 1:1,000 dilution in TBS-T with 5% BSA for 14-16 h at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... named as pS-SARS) and MERS-CoV S-expressing plasmid (Cat: VG40069-CF, named as pS-MERS) were obtained from Sino Biological Inc (Beijing ...