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Citations for Sino Biological :
601 - 650 of 1070 citations for SARS CoV 2 Spike E M Mosaic Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2023Quote: ... HRP-labeled Anti-His tag Antibody (105327-MM02T-H, Sino Biological) was then incubated at 37°C for an additional hour ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... R040 were raised against recombinant SARS-CoV nucleocapsid phosphoprotein (NP (Sino Biological, 0143-V08B) and expressed in HEK293 cells ...
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bioRxiv - Biophysics 2023Quote: ... were coated with protein A (Prospec, East Brunswick, NJ) and then with E-cadherin-Fc (Sino Biological, Beijing, China). To obtain the first coating with protein A ...
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bioRxiv - Microbiology 2022Quote: ... spleen and tracheobronchial lymph node were covered with a mouse monoclonal anti-SARS-CoV nucleocapsid protein (#40143-MM05, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at room temperature for forty five minutes ...
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bioRxiv - Biophysics 2020Quote: ... the substrate was incubated with 0.1 mg/ml recombinant E-cadherin-Fc for 2 hours (Sino Biological, Beijing, China). Afterwards ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-2-S2 (ECD; YP_009724390.1; Ser686-Pro1213; 40591-V08B) proteins were from Sino Biological. ITX 5061(HY-19900 ...
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bioRxiv - Microbiology 2021Quote: An immunohistochemistry (IHC) technique to detect SARS-CoV-2 nucleoprotein (NP) antigen using the rabbit monoclonal antibody (40143-R019, Sino Biological, Beijing, China) at a 1:15,000 dilution ...
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bioRxiv - Immunology 2021Quote: ... When this was done the mix was marked with rabbit monoclonal antibody anti-SARS-CoV-2 S1 (1:200) (cat. n° 40150-R007, Sino Biological, Beijing, China) as primary antibody for 1h at 37°C ...
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bioRxiv - Pathology 2021Quote: A previously described immunohistochemistry technique to detect SARS-CoV-2 NP antigen 40 using the rabbit monoclonal antibody (40143-R019, Sino Biological, Beijing, China) at dilution 1:1000 ...
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bioRxiv - Immunology 2021Quote: ... paraffin-embedded lung were incubated for 1 hour with the primary antibodies SARS-CoV-2 nucleoprotein (mouse IgG1, catalog number 40143-MM08, Sino Biological, Wayne, PA), Ionized calcium-binding adaptor (IBA1 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 3.7% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) and secondary antibody anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Immunology 2023Quote: The plasmid pCMV3-2019-nCoV-Spike (S1+S2)-long encodes full-length spike from SARS-CoV-2 amino acid 1–1273 (GenBank accession number YP_009724390 /QHD43416.1) and was obtained from Sino Biological (catalogue number VG40589-UT). This contained codon optimised cDNA for expression of the protein in mammalian cells inserted into the KpnI/XbaI sites of the mammalian expression vector pCMV3-untagged under control of the high-level expression mammalian human enhanced cytomegalovirus (CMV ...
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bioRxiv - Immunology 2023Quote: ... vaccines were formulated in 100 μL of PBS 1X and contained a 10 µg antigen dose of spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (Sino Biological 40589-V08H4) and a 20 µg 30% CpG-NPs adjuvant dose ...
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bioRxiv - Immunology 2020Quote: ... Mouse-Human chimeric mAb D001 (SARS-CoV RBD mAb; Sino Biological Inc Cat# 40150-D001) was used as a control mAb ...
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bioRxiv - Immunology 2022Quote: ... and incubated with cross-reactive rabbit anti-SARS-CoV-N IgG (Sino Biological, Inc., China) for 1 h at room temperature before adding an HRP-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Immunology 2020Quote: ... recombinant 2019-nCoV Spike protein (S1; Sino Biological), and recombinant 2019-nCoV Spike (S1-D614G ...
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bioRxiv - Immunology 2023Quote: Spike protein antigens were purchased from Sino Biological and stored at –20°C until utilized ...
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bioRxiv - Microbiology 2021Quote: ... and huCD4 (Met 1-Trp 390; catalog no. 10400-H08H) proteins with C-terminus 6x-His-tags were purchased from Sino Biological.
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bioRxiv - Molecular Biology 2021Quote: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with C terminal OFP Spark fluorescent tag (pCMV3-C-OFPSpark) was obtained from Sino biological (#VG40591-ACR). Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A ...
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bioRxiv - Immunology 2021Quote: ... were fixed after 48 hrs with 1:1 solution of methanol/ acetone and air-dried prior to immunostaining with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62; Sino Biological; 1:1000 dilution) and HRP-conjugated anti-Rabbit IgG antibody (1:2000 ...
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bioRxiv - Immunology 2021Quote: ... blocked with 5% skim milk in PBS-T and incubated with rabbit SARS-CoV-2 RBD polyclonal antibody (Cat # 40592-T62, Sino Biological; 1:1,000 dilution) overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Pathology 2020Quote: ... for 40 minutes, followed by a 60-minute incubation with the primary antibodies (SARS-CoV-2 nucleoprotein, mouse IgG1 (Sino Biological, cat#40143-MM08); ACE2 ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 antigen production in cells was detected by successive incubations with an anti-SARS-CoV nucleoprotein mouse monoclonal antibody (Sino Biological catalog# 40143-MM05), HRP IgG conjugate (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then fixed in 4% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171). Cells were then stained with anti-rabbit-IgG-FITC (Sigma ...
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bioRxiv - Immunology 2020Quote: ... CoV-2 S1 and S2 (Sino Biologicals) as well as influenza as control (Immune Tech) ...
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bioRxiv - Microbiology 2020Quote: ... mouse monoclonal anti-SARS-CoV NP at a dilution of 1:200 (40143-MM05, Sino Biological), mouse monoclonal anti-pan cytokeratin at a dilution of 1:100 (sc-8018 ...
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bioRxiv - Microbiology 2020Quote: The mouse mAb against the SARS-CoV nucleoprotein NP (40143-MM05) was purchased from Sino biologicals and used at dilutions of 1:500 for flow cytometry analysis and 1:1,000 for titration in TCID50 assays ...
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bioRxiv - Immunology 2022Quote: ... Anti-SARS-CoV-1 NP mouse monoclonal antibody (Sino Biological Inc.; Beijing, China, dilution: 1:500) and rabbit anti hamster IgA antibody (Brookwood Biomedical ...
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bioRxiv - Microbiology 2020Quote: ... The cells were then fixed and stained using a rabbit anti-SARS-CoV serum (Sino Biological) and a secondary peroxidase-labelled goat anti-rabbit IgG (Dako) ...
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bioRxiv - Microbiology 2020Quote: ... at dilution 1:500 and mouse monoclonal antibody against SARS-CoV NP (Sino Biological, 40143-MM05) at a dilution 1:500 for 2 h at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain was purchased from Sino Biological (RBD-Fc, Cat#: 40592-V05H). For kinetic interaction analysis of RBD escape mutants and sybodies ...
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bioRxiv - Microbiology 2021Quote: ... and 10% FBS in 0.1% Tween-20 PBS (PBST) for 1 h prior to staining with a rabbit polyclonal anti SARS-CoV-2 nucleocapsid antibody (Sino Biological Inc. catalog # 40588-T62) diluted 1:500 and incubated overnight at 4°C ...
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bioRxiv - Immunology 2020Quote: ... was quantitated then added at stoichiometric ratios (starting at 1:1) to 170 ng (750 fmol) of SARS-CoV-2 S1 trimer complex (Sino Biological, Cat: 40591-V08H; RU171) that had been dye-labeled with Dy-549P1 NHS ester in a total volume of 100 μL ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... except that HER2-His protein (Sino Biological) was used as the capture reagent for the total antibody and total ADC measurements.
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated with rabbit polyclonal antibody against SARS-CoV S (Sino Biological, #40150-T62-COV2) at dilution 1:500 and mouse monoclonal antibody against SARS-CoV NP (Sino Biological ...
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bioRxiv - Microbiology 2023Quote: ... the plates were sequentially stained with cross-reactive rabbit anti-SARS-CoV-N IgG (Sino Biological Inc) as the primary antibody and HRP-conjugated goat anti-rabbit IgG (H + L ...
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bioRxiv - Microbiology 2021Quote: ... Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000 ...
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bioRxiv - Microbiology 2020Quote: ... Specific anti-CoV immunoreactivity was detected using MERS-CoV nucleocapsid protein rabbit antibody (Sino Biological Inc.) at a 1:4000 ...
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bioRxiv - Immunology 2022Quote: ... Spike S1 protein from commercial sources (RayBiotech or Sino Biologicals) was used as indicated in the figure legends ...
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bioRxiv - Immunology 2021Quote: ... OC43-spike protein was purchased from Sino Biologicals (40607- V08B).
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bioRxiv - Immunology 2020Quote: ... or 1-10 μg of Spike RBD protein (Sino Biological, Cat ...
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bioRxiv - Immunology 2023Quote: Full-length spike protein antigens were purchased from Sino Biological, and the modified C.1.2 S1 protein was purchased from Medigen and stored at –20°C until utilized ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked with 5% milk in PBST before the membrane was incubated with a primary antibody (rabbit anti-SARS-CoV-2 S2 polyclonal antibody, Sino Biological, Cat# 40590-T62, 1:2500 dilution) in PBST ...
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bioRxiv - Microbiology 2020Quote: ... Positive control for the nucleocapsid ELISA was SARS-CoV nucleoprotein rabbit monoclonal antibody (Sino Biological Inc, Beijing, China). Negative controls were reagent grade human sera (compared to Mab CR3022) ...
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bioRxiv - Microbiology 2020Quote: ... the cells were fixed and stained with a polyclonal rabbit anti-SARS-CoV antibody (Sino Biological; 1:500). Staining was developed using a rabbit anti-SARS-CoV serum and a secondary alexa-fluor-labeled conjugate (Dako) ...
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bioRxiv - Immunology 2020Quote: ... and the number of infected cells was determined using SARS-CoV-S specific mAb (Sino Biological 401430-R001) and fluorescently labeled secondary antibody ...
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bioRxiv - Immunology 2020Quote: ... S2 ECD-His Recombinant Protein (Sino Biological, China), and RBD-His Recombinant Protein (Sino Biological ...