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Citations for Sino Biological :
601 - 650 of 840 citations for 4 2 Hydroxyimino 2 4 methoxy phenyl ethyl piperazine 1 carboxylic acid tert butyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... viral infection was examined by quantifying genomic and sub-genomic copy number by qPCR and immunofluorescence (IF) analysis using a SARS-CoV-2 antibody Polyclonal Anti-SARS Coronavirus antibody (Cat. # 40143-R001 from Sino Biological). SARS-CoV-2 antigen was detected in the cytoplasm of the infected cells ...
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bioRxiv - Microbiology 2024Quote: ... with or without rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc, Cat no. 40592-T62) and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Genetics 2020Quote: ... and incubated overnight in 500 µL of PBS/0.2% BSA containing 150 nM His6-tagged SARS-CoV-2 spike RBD (Sino Biological, Chesterbrook, PA) and 5 µg/mL anti-myc IgY (Aves Labs ...
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Bioengineering 2020Quote: ... and SARS-CoV-2 (2019-nCoV) and RBD of spike glycoprotein (mFC tag)(40592-V05H) were purchased from Sino Biological (Beijing, China).
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bioRxiv - Immunology 2021Quote: ... Cells were stained with an anti-SARS antibody (SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit monoclonal antibody; Sino Biological #40143-R001), diluted to 0.125 μg/mL in 3% BSA/PBS blocking solution for 1 h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... the plates were sequentially stained with cross-reactive rabbit anti-SARS-CoV-2 N IgG (Cat. No.: 40143-T62, Sino Biological Inc) as the primary antibody and HRP-conjugated goat anti-rabbit IgG(H+L ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then incubated with a rabbit polyclonal antibody against SARS-CoV/SARS-CoV-2-nucleoprotein (40143-T62, Sino Biological, Pennsylvania, USA) (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... isolated splenocytes were cultured with 10 mg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) (Sino biological Europe). Recombinant Ovalbumin [10 mg/ml] served as negative protein control ...
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Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: ... then proteins were monitored with ECL Western blotting substrate (Pierce) after incubation with anti-SARS-CoV-2-S-RBD (Sino Biological, China) or anti-β-actin antibody (Solarbio ...
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bioRxiv - Microbiology 2022Quote: ... after mounting slides were deparaffinized and antigen stained in house with a SARS-CoV-2 N specific antibody (Sino Biologicals #40143-R001) at a dilution of 1:30,000 followed by goat anti-rabbit secondary (Vector Labs #BA1000) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
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bioRxiv - Microbiology 2020Quote: ... The horses were inoculated subcutaneously with SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) antigens containing 1.0mg ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 8 μg/ml SARS-CoV-2 antigens (spike glycoprotein extracellular or receptor-binding domains, or nucleocapsid: Sino Biological, China) or SARS antigen (spike glycoprotein S1 subunit ...
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bioRxiv - Immunology 2021Quote: ... Plates were coated with 1μg/ml of SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1), SARS-CoV-2_DB_his (monomeric RBD ...
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bioRxiv - Immunology 2023Quote: ... each well was transfected with 2 μg eucaryotic recombinant plasmid pCMV3-C-GFPSpark (C-GFPSpark tag, Sino Biological Inc., Cat#VG40608-ACG) encoding the SARS-CoV-2 full-length membrane glycoprotein fused to a green fluorescent protein (GFP) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the SARS-CoV and SARS-CoV-2 S proteins were purchased from Sino Biologicals (catalog # VG40150-G-N; VG40589-CY). Expression of different coronavirus E proteins ...
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bioRxiv - Immunology 2023Quote: ... 26.4 pmol His-Tagged Biotinylated SARS-CoV-2 (2019-nCoV) spike RBD Recombinant Protein (Sino Biological Inc.; cat number: 40592-VO8H-B) was firstly incubated for 1 hour with 3.78 pmol Streptavidin Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Immunology 2022Quote: ... Antigen retrieval was performed using microwave heating (600 W) in 10 mM citric acid (pH 6.0) for 12 min for SARS-CoV-1 nucleoprotein antibody (Sino Biological Inc.; Beijing, China) and using recombinant protease from Streptomyces griseus (PanReac Applichem ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 with C terminal GFPSpark tag (pCMV3-hACE2-GFPSpark) was obtained from Sino biological (#HG10108-ACG). The mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike ...
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bioRxiv - Molecular Biology 2021Quote: Codon optimized SARS-CoV-2 (2019-nCoV) Spike S1 Gene with C terminal OFP Spark fluorescent tag (pCMV3-C-OFPSpark) was obtained from Sino biological (#VG40591-ACR). Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
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bioRxiv - Microbiology 2021Quote: An immunohistochemistry (IHC) technique to detect SARS-CoV-2 nucleoprotein (NP) antigen using the rabbit monoclonal antibody (40143-R019, Sino Biological, Beijing, China) at a 1:15,000 dilution ...
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bioRxiv - Bioengineering 2021Quote: ... was incubated with 5 ng/µl of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein (Sino Biological #40589-V08B1) in phosphate buffer (pH 6.0) ...
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bioRxiv - Microbiology 2020Quote: ... The SARS-CoV-2 S expression plasmid pC-SARS2-S was created by inserting the Acc65I/NotI-digested PCR-amplified SARS-CoV-2 S fragment of pCMV3-2019-nCoV-Spike(S1+S2)-long (Sino Biological; VG40589-UT) into the corresponding site of pCAGGS ...
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bioRxiv - Immunology 2021Quote: ... Potency was assessed using a cell-based immunosorbent assay to quantify infection by detecting the SARS-CoV-2 nucleoprotein using a specific antibody raised against this protein (Sino Biological, Wayne, PA). The secondary antibody (Life Technologies ...
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bioRxiv - Microbiology 2021Quote: ... anti-S1 neutralizing antibody (Catalog# 40591-MM43, lot HB14AP2001) and anti-S1 non-neutralizing antibody (Catalog # 40591-MM42, lot HB14AP0701) were also purchased from Sino Biological (Vendor #2). S1-Fc (Catalog # 10623-CV ...
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bioRxiv - Synthetic Biology 2022Quote: ... nanobodies were preincubated with 4 nM SARS-CoV-2 spike protein receptor binding domain fused to an Fc tag (RBD-Fc, SARS-CoV-2 spike protein residues 319-541) (Sino Biological, Wayne, PA) in PBS supplemented with 0.05 mg/mL BSA at 25°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated beads were incubated a second time with 2 µg of a recombinant human DC-SIGN protein with a human Fc tag (Sino Biological, Köln, Germany) for 1 h at 4°C with agitation ...
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bioRxiv - Pathology 2021Quote: A previously described immunohistochemistry technique to detect SARS-CoV-2 NP antigen 40 using the rabbit monoclonal antibody (40143-R019, Sino Biological, Beijing, China) at dilution 1:1000 ...
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bioRxiv - Biochemistry 2021Quote: ... different concentrations of rat serum (immunized with COVID-eVax vaccine) or anti-SARS-CoV-2 Spike S1 Subunit antibody (Sino Biological, 40150-T62) were added to each well and incubated overnight at 4°C in PBS – 0.05% Tween20 - 1% BSA ...
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bioRxiv - Immunology 2021Quote: ... HIV-1 PSD and pCMV3 containing the SARS-CoV-2/COVID-19 Spike gene were co-transfected into 7 x 105 293F cells using Sinofection (Sino Biological, Beijing, China). The medium was replaced with fresh DMEM containing 10% FBS after overnight incubation ...
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Cancer Biology 2023Quote: ... INHBA (Cat# 10429, -HNAH), and neuregulin-beta1 (Cat# 11609-HNCH, Ser 2-Lys 246 N-terminal fragment) were all from Sino Biological (Houston, TX). Human NRG1-specific siRNAs (5’-UCGGCUGCAGGUUCCAAAC ...
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bioRxiv - Cancer Biology 2023Quote: ... (2) VWF-bound gcEVs (VWF+gcEVs) obtained by incubation of gcEVs with 10 µg of recombinant (r) human VWF (Sino Biological, Beijing, China); (3 ...
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bioRxiv - Microbiology 2023Quote: ... The cells were fixed in 3.7% formaldehyde and stained with anti-SARS-CoV-2 polyclonal S1 antibody (Sino Biological, 40591-T62; RRID: AB_2893171) and secondary antibody anti-Rabbit-IgG-FITC (Sigma ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Microbiology 2020Quote: The pVSV-spike expression plasmid was constructed by PCR amplification of the full length human codon optimized spike gene from pCMV3-SARS-Cov-2 spike expression plasmid (Sino Biological, Cat #VG40588-UT) using the following primers ...
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bioRxiv - Microbiology 2022Quote: ... The Vero E6 cells were fixed and stained 20 hours (h) after infection and immunostained with a SARS-CoV/SARS-CoV-2 Nucleocapsid monoclonal antibody (Sino Biological, Wayne, PA, USA) followed by a horseradish peroxidase (HRP)-labeled Goat anti-Mouse IgG (cat # G-21040 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the pVSV-spike expression plasmid was constructed by PCR amplification of the full-length human codon-optimized S gene from pCMV3-SARS-CoV-2 S expression plasmid (Sino Biological, Cat# VG40588-UT) that was used to replace the VSV-G (Glycoprotein ...
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bioRxiv - Molecular Biology 2020Quote: ... 1A10 were prepared by immunizing BALB/c mice with recombinant SARS-CoV-2 RBD fused with a C-terminal mouse IgGFc tag (Sino Biological Inc, Beijing, China) using previously described protocols (Qu et al. ...
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bioRxiv - Genetics 2020Quote: ... Loaded sensors were dipped into recombinant SARS-Cov-2 His-tagged Spike protein (D614 or D614G, Sino Biological, Cat # 40591-V08H and 40591-V08H3).
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Pathology 2020Quote: ... for 40 minutes, followed by a 60-minute incubation with the primary antibodies (SARS-CoV-2 nucleoprotein, mouse IgG1 (Sino Biological, cat#40143-MM08); ACE2 ...
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bioRxiv - Immunology 2021Quote: ... sections were stained with an anti-SARS-CoV/SARS-COV-2 nucleocapsid (N) protein rabbit monoclonal antibody (#40143-R001, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at RT for 45 min ...
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bioRxiv - Microbiology 2020Quote: A full-length wild-type pCMV3-SARS-CoV-2 Spike (S1+S2)-long (termed as pCMV-S, codon-optimized, Sino Biological, cat # VG40589-UT) plasmid was used as a template to generate tagged pCMV-S ...
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bioRxiv - Immunology 2020Quote: ... RBD219-N1C1 was detected using a rabbit monoclonal antibody against the SARS-CoV-2 Spike S1 protein (Sino Biological, Beijing, China; Cat#: 40150-R007) and goat anti-rabbit IgG secondary antibodies conjugated with horseradish peroxidase (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Fractions which contain HexaPro protein were pooled and analyzed with SDS-PAGE and western blot against the SARS-CoV-2 RBD protein (Sino Biological, Cat#40592-T62) or pooled convalescent sera ...