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Citations for Sino Biological :
601 - 650 of 673 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... The cells were then incubated with 200 ng/well of R-PE labelled rabbit anti-SARS-CoV-2 spike neutralizing monoclonal antibody (Sino Biological, Cat: 40592-R001) at 4°C for 2 h ...
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bioRxiv - Immunology 2020Quote: ... SARS-CoV-2 antigen production in cells was detected by successive incubations with an anti-SARS-CoV nucleoprotein mouse monoclonal antibody (Sino Biological catalog# 40143-MM05), HRP IgG conjugate (Jackson ImmunoResearch Laboratories ...
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bioRxiv - Cell Biology 2022Quote: ... BMDMs were plated in 6-well plates (2×105 cells/well) and treated with 1 ng/mL LPS and 20 ng/mL mIFNγ (Sino Biological Inc., Beijing, China) for 24 h ...
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bioRxiv - Bioengineering 2023Quote: ... The sample solutions were prepared by spiking biotinylated SARS-CoV-2 N protein rabbit monoclonal antibody (no. 40143-R004-B; Sino Biological, Inc.; Beijing, China) in buffer solution (0.1% BSA and 0.05% Tween 20 in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... consisting of SARS-CoV-2 residues Arg319—Phe541 fused to a murine IgG1 Fc domain was purchased from Sino Biological (RBD-Fc, Cat#: 40592-V05H). For kinetic interaction analysis of RBD escape mutants and sybodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were permeabilised in methanol 0.6% H2O2 and stained for 1 h with an antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; 40143-R019, 1:300 dilution). Cells were further stained with the secondary antibody anti-rabbit HRP conjugate (Sigma ...
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bioRxiv - Microbiology 2021Quote: ... and 10% FBS in 0.1% Tween-20 PBS (PBST) for 1 h prior to staining with a rabbit polyclonal anti SARS-CoV-2 nucleocapsid antibody (Sino Biological Inc. catalog # 40588-T62) diluted 1:500 and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... the sections was incubated with corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:200; anti-SARS-CoV-2 Spike antibody, 40150-R007, Sino Biological, China, dilution 1:400) for 16 hours at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... the membrane was incubated with the corresponding primary antibodies (anti-CD147, HAb18, produced by our laboratory, dilution 1:2000; anti-SARS-CoV-2 Spike antibody, 40150-R007, Sino Biological, China, dilution 1:2000) at 4°C overnight ...
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bioRxiv - Microbiology 2020Quote: ... Chamber slides were washed twice with PBS-tween and then incubated with primary antibody SARS-CoV-2 spike chimeric monoclonal antibody (Sino Biological #40150-D004, Beijing, China) diluted 1:1,000 in PBSK for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... followed by rabbit monoclonal antibody against SARS-CoV-2 N protein (1:20,000 dilution x 60 minutes, #40143-R019, Sino Biological US Inc., Wayne, PA, USA), then incubated with Rabbit Envision (Dako ...
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bioRxiv - Microbiology 2021Quote: ... For the detection of Spike protein-similar structures the samples were first incubated in 50 μL containing 0.5 μg SARS-CoV-2 Spike Neutralizing Antibody (clone: HA14JL2302, Sino Biological Inc. Cat. No: 40592-R001) or Neutralizing Antibody isolated from COVID-19 patients for 15 min at 4 °C then washed with PBS and stained again in 50 μL of the anti-Rabbit Alexa 647 (7,5μg/ml ...
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bioRxiv - Immunology 2020Quote: ... was quantitated then added at stoichiometric ratios (starting at 1:1) to 170 ng (750 fmol) of SARS-CoV-2 S1 trimer complex (Sino Biological, Cat: 40591-V08H; RU171) that had been dye-labeled with Dy-549P1 NHS ester in a total volume of 100 μL ...
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bioRxiv - Immunology 2023Quote: ... Paraffin blocks containing the left lung lobe were sectioned at approximately 4 microns and stained using hematoxylin and eosin (H&E) or immunostained using a rabbit monoclonal antibody against SARS-CoV-2 nucleocapsid protein (Sino Biological; cat. no. 40588-R002) and rabbit polyclonal antibodies against CD-3 protein (Dako ...
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bioRxiv - Cancer Biology 2023Quote: A549 cells were seeded in 60 mm plates then transfected with 2 µg of N-terminal tagged GFP-HMGB1 DNA plasmid DNA (Sino biological, cat no. HG10326-ANG). In brief ...
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bioRxiv - Molecular Biology 2024Quote: The PPAP isoform 2 (membrane protein) protein tagged with HA in the C terminus was expressed using a commercially available plasmid from Sino Biologicals (HG10959-CY, Sino Biologicals). The plasmid was transfected into HeLa 229 cells using X-tremeGENE™ HP DNA Transfection Reagent (6366236001 ...
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Bioengineering 2021Quote: ... washed five times with PBST and incubated at room temperature for 1 h with 100 µL of 1 µg/mL anti-SARS-CoV-2 spike rabbit polyclonal antibody (Sino Biological, Beijing, China, Cat#40589-T62) in PBSCT ...
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bioRxiv - Immunology 2021Quote: ... the cells were permeabilized with Triton X-100 and probed with a SARS-CoV/SARS-CoV-2 nucleoprotein-specific rabbit primary antibody (Sino Biological, Wayne, PA, USA, 40143-R001) followed by an Alexa Fluor 647-conjugated secondary antibody (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... and stained with Coomassie Blue or transferred to a polyvinylidene difluoride membrane and probed with a monoclonal anti-SARS-CoV-2 Spike rabbit antibody recognizing the RBD region (Sino Biological, Beijing, China; Cat # 40150-R007) to evaluate the size and confirm the identity ...
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bioRxiv - Microbiology 2021Quote: ... 96-well plates were coated overnight with either 1 μg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (RBD, His Tag, Sino biological, Cat. No. 40592-V08B-100) or recombinant SARS-CoV-2 Nucleocapsid His Protein ...
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bioRxiv - Microbiology 2021Quote: ... SARS-CoV-2 His-tagged Spike (S) protein and the receptor binding domain (RBD) protein of S were purchased from Sino Biological (Beijing/China and Wayne/USA).
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bioRxiv - Bioengineering 2023Quote: Nectagen’s nanoCLAMP phage display library NL-21 was panned as described (Suderman et al. 2017) against SARS CoV 2 Spike protein RBD (Sino Biologicals, S1RBD-Fc, cat#40592-VO2H) for rounds 1 and 2 ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked with 5% skimmed milk in PBS for 1 hour and incubated with a SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc., Beijing, China, Cat: 40592-T62) for another hour at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The membrane was blocked with 5% milk in PBST before the membrane was incubated with a primary antibody (rabbit anti-SARS-CoV-2 S2 polyclonal antibody, Sino Biological, Cat# 40590-T62, 1:2500 dilution) in PBST ...
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bioRxiv - Immunology 2023Quote: ... human CTLA-4 (Sino Biological, 11159-HNAH), and human FcyRs (human FCGR2A #10374-H08H ...
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bioRxiv - Microbiology 2020Quote: ... SARS-CoV2-S1 (Sino Biological, 40591-V08H, n=3) and SARS-CoV2-S2 (Sino Biological ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Human recombinant Galectin 3 (His tag) (Sino Biological Inc.) in PBS was then applied to the wells coated with svPDE after the wells were washed with PBST three times ...
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bioRxiv - Bioengineering 2023Quote: Binding of IL-3 (Sino Biological, PN: 11858-H08H) (analyte in solution ...
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bioRxiv - Microbiology 2020Quote: ... and SARS-CoV2-S2 (Sino Biological, 40590-V08B, n=3), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... biotinylated HER2 ectodomain (4 μg/mL, Sino Biological, Beijing, China) was added to the tube and incubated for 2 h at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... Omicron BA.4/BA.5 (Sino Biological, cat. 40589-V08H32), Omicron XBB (Sino Biological ...
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bioRxiv - Immunology 2023Quote: ... rhIL-4 (GMP-11846-HNAE) was purchased from Sino Biological. Human IFN-γ ELISA Set (555142 ...
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bioRxiv - Biophysics 2024Quote: ... and fixed with 4% paraformaldehyde (Sino Biological. Inc., Beijing, China). SARS-CoV-2 S proteins induced by vaccines on the cell membrane were recognized by S309 primary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... astrocyte cultures were treated with 3 ng/ml IL-1α (Sino Biological, 50114MNAE), 30 ng/ml TNF-α (PeproTech ...
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bioRxiv - Bioengineering 2020Quote: ... PD-L1 and CTLA-4 were purchased from Sino Biological (Beijing, China). The CHO-PD-L1-CD3L cell and Jurkat-PD-1-NFAT cell line were provided by National Institutes for Food and Drug Control (short for NIFDC ...
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bioRxiv - Immunology 2021Quote: ... OX40 and 4-1BB reporter constructs were cloned from vectors purchased from Sino Biological. Sequences were confirmed by automated capillary Sanger sequencing (Macrogen Europe).
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bioRxiv - Microbiology 2023Quote: ... Primary antibody incubation was conducted for 3 hours at room temperature (1:2,000 α-N protein, Sino Biological 40143-R001 ...
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bioRxiv - Immunology 2021Quote: ... washed with PBS and coated overnight at 4°C with 1μg/ml of nucleoprotein (Sino Biological) diluted in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... coated overnight at 4°C with 100 μg/mL of ACE2 protein (Sino Biological, 10108-H08H) that was diluted in PBS (pH 7.4) ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 1 µg/mL purified S1+S2 antigen (Sino Biological) in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... Growth factors and cytokines such as M-CSF and IL-4 were obtained from Sino Biologicals, CellTracker live cell stains (CellTracker Red ...
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bioRxiv - Immunology 2023Quote: ... The mixture was incubated at 37℃ for 1 hour and added to 3×104 HEK293T-hACE2 cells (Sino Biological). After 48h incubation ...
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Notch signaling functions in non-canonical juxtacrine manner in platelets to amplify thrombogenicitybioRxiv - Cell Biology 2022Quote: ... DLL-1 (# 11635-H08H) and DLL-4 (# 10171-H02H) recombinant human proteins were products from Sino Biological. Anti-CD62P (# 550561) ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated human IL-2Rβ (Acro Biosystems, ILB-H82E3) and human 4-1BB (Sino Biological, 10041-H27H-B) were diluted to a concentration of 2.5 μg/mL in the kinetics buffer and loaded onto streptavidin-coated biosensors (SAForteBio) ...
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bioRxiv - Biochemistry 2020Quote: ... Approximately 1.5 mg plasmids were incubated with 3 mg PEIs in 50 mL SMM 293T-I medium (Sino Biological Inc.) for 30 min before transfection ...