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Citations for Sino Biological :
551 - 600 of 668 citations for tert Butyl 3 5 2 amino ethyl thiophen 3 yl propionate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: The rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Cat# 40592-T62) or human SARS-CoV-2 S-NTD antibody (E-AB-V1030) was obtained from Sino Biological or Elabscience ...
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bioRxiv - Immunology 2020Quote: ... Nbs were incubated with the bead-mix (containing beads coupled with SARS-CoV-2 homotrimeric spike, RBD and S1 proteins) and biotinylated ACE2 (Sino Biological) which competes for the binding of SARS-CoV-2 spike-derived antigens ...
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
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bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Biochemistry 2022Quote: ... Codon-optimized expression vactors for HA-tagged and GFP-tagged nucleocapsid (N) protein of SARS-CoV-2 virus were purchased from Sino Biologicals US Inc ...
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bioRxiv - Microbiology 2022Quote: ... The vectors expressing the SARS-CoV-2 M and S proteins (with C-terminal HA-tag) were obtained from Sino Biologicals. The pcDNA3.1 (+)-N-eGFP-N plasmid ...
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bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
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bioRxiv - Immunology 2022Quote: Plasma or memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2022Quote: ... purified SARS-CoV-2 spike-specific monoclonal rabbit primary antibodies R001 and R007 (cat. n° 40592-R001 and cat. n° 40150-R007, Sino Biological)
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bioRxiv - Immunology 2022Quote: ... Plates were coated with 1 µg/ml of SARS-CoV-2 (2019-nCoV) spike RBD-His (Sino Biologicals, Cat. 40592-V08B) for 4-5h at 37°C and then blocked overnight in 1% BSA ...
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bioRxiv - Immunology 2022Quote: ... Transfected HEK293T cells were stained with Fixable Viability Dye (eBioscience) and incubated with mouse Fc-tagged recombinant human ACE-2 (Sino Biological). A secondary donkey anti-mouse antibody conjugated with AF647 was used for detection of surface expression ...
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bioRxiv - Immunology 2024Quote: ... were coated with 100 ng/well of SARS-CoV-2 (2019-nCOV) Spike S1 + S2 ECD-His-Recombinant Protein (40589-V08B1, Sino Biological) overnight at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Various dilutions of SEC-purified S-EABR eVLP samples and known amounts of soluble SARS-CoV-2 S1 protein (Sino Biological) were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Microbiology 2022Quote: The following antibodies were used for flow cytometry: rabbit monoclonal SARS-CoV-2 spike-specific antibody [R001] (Cat. n° 40592-R001, Sino Biological), mouse monoclonal SARS-CoV-2 spike-specific antibody [MM57] (cat ...
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bioRxiv - Microbiology 2022Quote: ... anti-SARS-CoV-2 binding IgG levels were reported in units of μg/mL based on a standard curve that was generated using a SARS-CoV-2 spike nAb (Cat# 40595-MM57, Sino Biological).
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was coated with 2 µg/mL spike antibody overnight at 4°C (Cat# 41050-D003, Sino Biological). The plate was then washed with ELISA wash buffer and blocked with 300 µL/well blocking buffer at room temperature for 1 hour and washed again ...
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bioRxiv - Microbiology 2023Quote: ... The antibodies used in this study included rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Sino Biological, Cat# 40150-R007), mouse monoclonal anti-SARS-CoV-2 S2 antibody (Abcam ...
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bioRxiv - Immunology 2023Quote: Memory B cell culture supernatants were diluted in PBS and mixed with SARS-CoV-2 RBD mouse Fc-tagged antigen (Sino Biological, 40592-V05H ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Bioengineering 2023Quote: ... 96-well plates were coated with 50 μL of coating buffer containing 100 ng/well recombinant SARS-CoV-2 spike antigens (Sino Biological) at 4 °C overnight ...
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bioRxiv - Cell Biology 2024Quote: ... viral infection was examined by quantifying genomic and sub-genomic copy number by qPCR and immunofluorescence (IF) analysis using a SARS-CoV-2 antibody Polyclonal Anti-SARS Coronavirus antibody (Cat. # 40143-R001 from Sino Biological). SARS-CoV-2 antigen was detected in the cytoplasm of the infected cells ...
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bioRxiv - Immunology 2024Quote: ... plates were then incubated with 25 μl/well of a 1 μg/ml solution of recombinant antibody or control antibodies (anti-PTx, Pertussis Toxinm, Ab49, in-house; Anti SARS-CoV-2 S RBD, 40592-R001, Sino Biological) was applied and incubated for 2 hours at room temperature ...
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bioRxiv - Microbiology 2024Quote: ... with rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc, Cat. no. 40592-T62, 1: 1000) and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2024Quote: ... and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike S2 Antibody (Sino Biological Inc, Cat. no. 40590-T62, 1: 1000) or N protein of SARS-CoV-2 (dilution 1:2000 ...
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bioRxiv - Microbiology 2024Quote: ... with or without rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV) Spike RBD Antibody (Sino Biological Inc, Cat no. 40592-T62) and rabbit polyclonal anti-SARS-CoV-2 (2019-nCoV ...
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bioRxiv - Microbiology 2024Quote: ... Slides were incubated with a rabbit polyclonal antibody against SARS-CoV/SARS-CoV-2-nucleoprotein (40143-T62, Sino Biological, 1:1000), rabbit anti-IBA1 (019-19741 ...
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bioRxiv - Bioengineering 2023Quote: ... cells were stimulated using a half media change with a final cytokine cocktail of 5 ng/mL IFN-ү/TNF-α (Gibco, Sino Biological, respectively). MSC-EV treatment groups received an additional 10 uL of MSC-EV preparation for the 1X dilution ...
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bioRxiv - Genetics 2020Quote: ... and incubated overnight in 500 µL of PBS/0.2% BSA containing 150 nM His6-tagged SARS-CoV-2 spike RBD (Sino Biological, Chesterbrook, PA) and 5 µg/mL anti-myc IgY (Aves Labs ...
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Bioengineering 2020Quote: ... and SARS-CoV-2 (2019-nCoV) and RBD of spike glycoprotein (mFC tag)(40592-V05H) were purchased from Sino Biological (Beijing, China).
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bioRxiv - Immunology 2021Quote: ... Cells were stained with an anti-SARS antibody (SARS-CoV/SARS-CoV-2 nucleocapsid antibody, rabbit monoclonal antibody; Sino Biological #40143-R001), diluted to 0.125 μg/mL in 3% BSA/PBS blocking solution for 1 h at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... cells were incubated for 2 h at room temperature with mouse anti-MERS-CoV N IgGs (1:000 dilution, Sino Biological, China), followed by 1 h of incubation with Alexa Fluor 488-labeled goat anti-mouse IgG (2.5 µg/ml ...
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bioRxiv - Microbiology 2022Quote: ... the plates were sequentially stained with cross-reactive rabbit anti-SARS-CoV-2 N IgG (Cat. No.: 40143-T62, Sino Biological Inc) as the primary antibody and HRP-conjugated goat anti-rabbit IgG(H+L ...
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bioRxiv - Microbiology 2022Quote: ... Slides were then incubated with a rabbit polyclonal antibody against SARS-CoV/SARS-CoV-2-nucleoprotein (40143-T62, Sino Biological, Pennsylvania, USA) (1:1000) ...
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bioRxiv - Immunology 2021Quote: ... isolated splenocytes were cultured with 10 mg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) (Sino biological Europe). Recombinant Ovalbumin [10 mg/ml] served as negative protein control ...
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Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: ... then proteins were monitored with ECL Western blotting substrate (Pierce) after incubation with anti-SARS-CoV-2-S-RBD (Sino Biological, China) or anti-β-actin antibody (Solarbio ...
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bioRxiv - Microbiology 2022Quote: ... after mounting slides were deparaffinized and antigen stained in house with a SARS-CoV-2 N specific antibody (Sino Biologicals #40143-R001) at a dilution of 1:30,000 followed by goat anti-rabbit secondary (Vector Labs #BA1000) ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
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bioRxiv - Microbiology 2020Quote: ... The horses were inoculated subcutaneously with SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) antigens containing 1.0mg ...
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bioRxiv - Microbiology 2020Quote: ... were coated with 8 μg/ml SARS-CoV-2 antigens (spike glycoprotein extracellular or receptor-binding domains, or nucleocapsid: Sino Biological, China) or SARS antigen (spike glycoprotein S1 subunit ...
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bioRxiv - Immunology 2021Quote: ... the viral nucleocapsid was detected using 1:5000 of SARS-CoV/SARS-CoV-2 Nucleocapsid monoclonal antibody (Sino Biological, Cat#40143-R001) by incubation at 37°C for 1 h ...
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bioRxiv - Immunology 2021Quote: ... Plates were coated with 1μg/ml of SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1), SARS-CoV-2_DB_his (monomeric RBD ...
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bioRxiv - Immunology 2022Quote: ... and SARS-CoV-1 pseudotyped virus neutralization assay) or 293T cells overexpressing human angiotensin-converting enzyme 2 (293T-hACE2) (Sino Biological Company) (for Pangolin-GD and RaTG13 pseudotyped virus neutralization assay) ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the SARS-CoV and SARS-CoV-2 S proteins were purchased from Sino Biologicals (catalog # VG40150-G-N; VG40589-CY). Expression of different coronavirus E proteins ...
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bioRxiv - Immunology 2023Quote: ... each well was transfected with 2 μg eucaryotic recombinant plasmid pCMV3-C-GFPSpark (C-GFPSpark tag, Sino Biological Inc., Cat#VG40608-ACG) encoding the SARS-CoV-2 full-length membrane glycoprotein fused to a green fluorescent protein (GFP) ...
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bioRxiv - Immunology 2023Quote: ... 26.4 pmol His-Tagged Biotinylated SARS-CoV-2 (2019-nCoV) spike RBD Recombinant Protein (Sino Biological Inc.; cat number: 40592-VO8H-B) was firstly incubated for 1 hour with 3.78 pmol Streptavidin Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Bioengineering 2023Quote: ... The libraries were subjected to five rounds of biopanning against the recombinant SARS-CoV-2 BA.1 RBD protein (Sino Biological Inc.) as described previously38 ...
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bioRxiv - Molecular Biology 2021Quote: ... Full-length DNA clone of Human angiotensin I converting enzyme (peptidyl-dipeptidase A) 2 with C terminal GFPSpark tag (pCMV3-hACE2-GFPSpark) was obtained from Sino biological (#HG10108-ACG). The mammalian expression plasmid for RBD of SARS-CoV-2 protein S (spike ...