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Citations for Sino Biological :
451 - 500 of 510 citations for Recombinant Human ABHD15 His tagged since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... 50 ng Firefly-luciferase (FLuc) reporter constructs were mixed with storage buffer or storage buffer supplemented with recombinant 4E-BP1 (Sino Biological, 10022-H07E), eIF4A1wt or eIF4A1E183Q (eIF4A1DQAD ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...
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bioRxiv - Immunology 2021Quote: 96-well microtiter plates were coated over night at 4°C with 100ng per well of recombinant NTD (Sino Biological; Beijing, China #40591-V49H), SARS-CoV-2 Spike S2 or S1 Glycoprotein (Virion\Serion ...
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bioRxiv - Molecular Biology 2020Quote: ... 1A10 were prepared by immunizing BALB/c mice with recombinant SARS-CoV-2 RBD fused with a C-terminal mouse IgGFc tag (Sino Biological Inc, Beijing, China) using previously described protocols (Qu et al. ...
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bioRxiv - Immunology 2020Quote: ... and ADI-56046) were subject to two rounds of selection for binding to a recombinant SARS-CoV-2 S1 protein (Sino Biological, Cat # 40591-V08H). Induced yeast libraries covering at least 10-fold of their respective diversities were incubated with 10 or 1 nM biotinylated SARS-CoV-2 S1 protein under equilibrium conditions ...
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bioRxiv - Cell Biology 2023Quote: Cells were treated with either the recombinant gp120 of HIV-1 Group M Subunit A (Catalog number 40403-V08H, Sino Biological, Inc., Beijing, China), gp120 of HIV-2 Group M Subunit B (Catalog number 40404-V08H ...
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bioRxiv - Microbiology 2021Quote: The human ACE2 protein was purchased from Sino Biological (Beijing, China; Cat# 10108-H08H) and the human IgG1 Fc-tagged RBD proteins were made in-house using a method as previously described35 ...
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bioRxiv - Biochemistry 2021Quote: The cDNA for human ACE2 (Uniprot: Q9BYF1) was purchased from Sino Biological (HG10108-M). cDNAs encoding the SARS-CoV-2 genes for ORF6 ...
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bioRxiv - Biochemistry 2021Quote: ... Full-length human MAVS cDNA was transferred from pGEM- MAVS (Sino Biological HG15224-G) into the KpnI-NotI sites of the pCMV6-Entry vector (OriGene Technologies #PS100001 ...
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bioRxiv - Microbiology 2021Quote: The human ACE2 protein was purchased from Sino Biological (Beijing, China; Cat# 10108-H08H) and the human IgG1 Fc-tagged RBD proteins were made in-house using a method as previously described28 ...
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bioRxiv - Immunology 2020Quote: The MerTK (Mer cDNA ORF Clone, Human, untagged, pCMV3) was obtained from Sino Biological. Both pcDNAI-GAL4-CREB and 5xGAL4-TATA-luciferase were a gift from Richard Maurer(Sun et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The pCMV3-N-Myc-human AXL (HG10279-NM) plasmid was purchased from Sino Biological. The peGFP-C1-KRas4A ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with 100nM human or mouse PC (HTI and Sino Biologicals, respectively) and 5nM thrombin (HTI ...
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bioRxiv - Biophysics 2021Quote: For affinity measurements of anti-human coronavirus spike glycoprotein HKU1 (40021-MM07-100, Sino Biological) to HCoV-HKU1 S1 protein ...
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bioRxiv - Cancer Biology 2021Quote: ... Ligand blocking was performed by pre-adsorbing HPK with soluble human HER3 peptide (Sino Biological) at 10× molar excess in PBS before adding to cells ...
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bioRxiv - Immunology 2020Quote: ... Mouse-Human chimeric mAb D001 (SARS-CoV RBD mAb; Sino Biological Inc Cat# 40150-D001) was used as a control mAb ...
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bioRxiv - Bioengineering 2021Quote: ... wells were washed with PBS-T and further incubated with human ACE2-Fc (Sino Biological) for 2 hours at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Antibodies against human PCNA (proliferating cell nuclear antigen, rabbit) were purchased from Sino Biological (China). DNase I recombinant RNase free was purchased from Roche Life Sciences (Germany).
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bioRxiv - Developmental Biology 2024Quote: The I-BAR domain of human Mtss1 was purchased from Sino Biological (Cat 13085-H10E). The human Plexin-D1 cytosolic domain was prepared ...
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bioRxiv - Biochemistry 2023Quote: ... Expression plasmids in human cells of untagged RFPL4 and PTOV1 were purchased from Sino Biological (products HG25044-UT and #HG20551-UT ...
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bioRxiv - Immunology 2021Quote: ... 96 well NUNC MaxSorb flat-bottom plates were coated with 3.99 μg/ml recombinant SARS-CoV-2 Wuhan-Hu-1 spike RBD protein (Sino Biological, Beijing, PR China, Cat. 40592-V08B), 1 μg/ml SARS-CoV-2 Wuhan-Hu-1 nucleocapsid protein (Sino ...
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bioRxiv - Immunology 2024Quote: ... 300 response units (RU) of IFN-α (human, rhesus, or mouse untagged, Sino Biological, Chesterbrook, PA) or 500 RU of IFN-β (human ...
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bioRxiv - Immunology 2022Quote: ... HEK 293T cells were transiently transfected with a plasmid expressing human DC-SIGN (HG10200-UT; Sino Biological), using Promega FuGENE™ HD Transfection Reagent (Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... and human HepaCAM protein extracellular domain (ECD, amino acid sequence 1-240, 16047-H08H) (Sino Biological Inc.) were used in this study ...
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bioRxiv - Bioengineering 2023Quote: ... Human polymeric immunoglobulin receptor (hpIgR) construct was assembled from a cDNA clone (Sino Biological, cat. HG10131-UT), which was used to extract the fragment encoding the hpIgR ectodomain ( GenBank:NP_002635 ...
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bioRxiv - Biochemistry 2024Quote: Human eEF2K cDNA clone in pGEM-T cloning vector was purchased commercially (Sino Biological Cat: HG11587-G) and resuspended in 100 uL of sterile water ...
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bioRxiv - Bioengineering 2024Quote: NUNC MaxiSorp ELISA plates were coated with 0.5 μg/mL human CD64 (Sino Biological, Catalog# 10256-H08H) in 50 mM carbonate-bicarbonate buffer ...
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bioRxiv - Microbiology 2021Quote: ... we screened preconstructed human naïve phage scFv libraries with biotinylated SARS-CoV-2 RBD (Sino Biological, 40592-V05H). Phage libraries were constructed using antibody gene fragments amplified from PBMCs of 50 healthy human subjects (Allcells ...
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bioRxiv - Bioengineering 2023Quote: Full length cDNA of human CD123 (NM_002183.2) was obtained from a pCMV3 vector (Cat#HG10518-M, Sino Biological). The Hygromycin sequence was replaced by a Neomycin resistance cassette by Gibson Assembly ...
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bioRxiv - Neuroscience 2022Quote: ... Anti-SARS-CoV-2 nucleocapsid (Rabbit Anti-Human, Citrate Buffer HIER, dilution 1:7000, Sino Biologicals, 40143-R001) and -Spike Subunit 1 Antibody (Monoclonal Rabbit Anti-Human ...
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bioRxiv - Genetics 2020Quote: Mammalian cDNA ORF clones were used for AVP (human, HG17671-UT, NCBI Ref Seq: NM_000490.4, Sino Biological, Beijing, China) and MAOA (mouse ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA fragments encoding human hSSB1 and INTS3 were amplified by PCR from NABP2- GFPspark plasmid (Sino Biological, HG22790-ACG) and INTS3- GFPspark plasmid (Sino Biological ...
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bioRxiv - Cancer Biology 2023Quote: ... was coated overnight with 100 ng/well (in 100 μL/well volume) of purified human CEACAM6 protein (Sino Biological). Standards containing known concentrations of the UCHT-2-03 recombinant protein or test samples were applied to the plate for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... The spike expression on cell surface were detected by staining cells with human ACE2–Fc chimera (Sino Biological, 10108-H02HG) in MACS buffer (D-PBS with 2 mM EDTA and 0.5% BSA ...
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bioRxiv - Biochemistry 2021Quote: Vero E6 cells were incubated with RBD protein (0.45 μg/mL, final concentration) followed by incubation with human anti-RBD antibody (primary antibody) (40150-D003, Sino Biological) and goat anti-human IgG AF488-conjugated antibody (secondary antibody ...
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bioRxiv - Biochemistry 2024Quote: ... Full-length monoclonal antibodies (mAb) and antibody fragments (Fab) were produced recombinantly in human cells by Sino Biological (Wayne, PA). Fabs were made from mAbs by papain protease digestion ...
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bioRxiv - Genomics 2024Quote: The human codon-optimized full-length SARS-CoV-2 spike (Wuhan-Hu-1 strain) gene was obtained from Sino Biological Inc ...
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bioRxiv - Biophysics 2024Quote: The genes encoding wild-type full-length human PDGFRβ (from Dr. Jiahuai Han laboratory) and CSF1R (from Sino Biological Inc) were subcloned into the pEF1-puro plasmid (from Dr ...
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bioRxiv - Biochemistry 2020Quote: A Pro-CTSL clone was generated by sub-cloning the human Pre-Pro-CTSL ORF clone (in pMD18-T Simple Vector) obtained from Sino Biologicals Inc. ...
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bioRxiv - Microbiology 2021Quote: ... The binding of hACE2 to surface expressed S protein on infected HeLa cells was detected by incubating with 100 ng/106 cells of biotinylated human ACE2 protein (Cat# 10108-H08H-B, Sino Biological) followed by Alexa Fluor 647-conjugated anti-hACE2 antibody (Cat# FAB9332R ...
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bioRxiv - Microbiology 2021Quote: ... The cDNA of human CD47 and mouse CD47 with an HA-tag at the C-terminal were purchased from Sino Biological and Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... Similarly human ACE2 binding to surface expressed spike on MVA/S infected DF-1 cells was done using biotinylated human ACE2 protein (#10108-H08H-B, Sino Biological) followed by streptavidin-PE (BD Pharmingen ...
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bioRxiv - Microbiology 2021Quote: The rabbit polyclonal antibody against SARS-CoV-2 SP/RBD (Cat# 40592-T62) or human SARS-CoV-2 S-NTD antibody (E-AB-V1030) was obtained from Sino Biological or Elabscience ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Evolutionary Biology 2023Quote: The plasmid containing the human SLC30A9 gene codifying for the ZnT9 transporter with an N-HA tag was obtained from Sino Biological Inc ...
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bioRxiv - Microbiology 2021Quote: ... of the spike protein (S-RBD) of the SARS-CoV-2 virus (with human Fc as a tag for detection) was purchased from Sino Biological (Beijing).
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bioRxiv - Cancer Biology 2021Quote: pCMV3-N-HA negative control vector and human RPRM plasmids (full length, S98A mutant and Δ(79-109) mutant) were purchased from Sino Biological Inc ...
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bioRxiv - Bioengineering 2020Quote: ... Human scFv phage libraries were incubated with a mixture of 6 other coronaviruses for negative selection: SARS-CoV (Sino biological, 40150-V08B1), MERS-CoV (Sino biological ...
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bioRxiv - Cancer Biology 2020Quote: ... Prepared elastic Fab anti-Fc-functionalized PAA nanotextures were incubated with 20 μg/mL human ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (4°C ...
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bioRxiv - Biochemistry 2021Quote: Affinity-purified full-length EGFR was concentrated to 50 μM and treated with 100 μM human EGF (Novoprotein) or TGF-α (Sino Biological) for at least 5 mins on ice ...