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Citations for Sino Biological :
451 - 500 of 519 citations for Dog Trefoil Factor 3 TFF3 Protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... of the following SARS-CoV-2 derived antigens: S1+S2 subunits of the Spike (S) protein and receptor-binding domain (RBD, Sino Biological) were subsequently added and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with 3% BSA-PBS for 30 min to block nonspecific antibody binding and then incubated with SARS-CoV-2 nucleocapsid protein antibody (Sino Biological), followed by incubating with Alexa Fluor 488 (Thermo) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... two New Zealand white rabbits were immunized subcutaneously with 0.5 mg recombinantly expressed human Chi3l1 protein (Sino Biological, Cat: 11227-H08H). After the initial immunization ...
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bioRxiv - Microbiology 2021Quote: ... Calu3 cells were fixed for 30 min in 4 % paraformaldehyde (PFA) and stained with a polyclonal antibody targeting the SARS-CoV-2 Nucleocapsid (N) protein (Sino Biological, catalogue number ...
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bioRxiv - Immunology 2022Quote: ... Various dilutions of SEC-purified S-EABR eVLP samples and known amounts of soluble SARS-CoV-2 S1 protein (Sino Biological) were separated by SDS-PAGE and transferred to nitrocellulose membranes (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... IHC was carried out using a rabbit monoclonal antibody for SARS-CoV nucleocapsid protein (catalogue #40143-R001, clone #001, Sino Biological) which cross-reacts with SARS-CoV-2 nucleocapsid protein ...
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bioRxiv - Immunology 2024Quote: ... were coated with 100 ng/well of SARS-CoV-2 (2019-nCOV) Spike S1 + S2 ECD-His-Recombinant Protein (40589-V08B1, Sino Biological) overnight at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... Confirmation of the S expression in HEK293T transfected cells was performed by Western blot using antibody against SARS-CoV-2 S2 protein (Sino Biological).
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bioRxiv - Biochemistry 2022Quote: ... Codon-optimized expression vactors for HA-tagged and GFP-tagged nucleocapsid (N) protein of SARS-CoV-2 virus were purchased from Sino Biologicals US Inc ...
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bioRxiv - Microbiology 2022Quote: ... The vectors expressing the SARS-CoV-2 M and S proteins (with C-terminal HA-tag) were obtained from Sino Biologicals. The pcDNA3.1 (+)-N-eGFP-N plasmid ...
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bioRxiv - Microbiology 2022Quote: ... The copies of the phage-packaged NP protein were quantified by Western blotting using the commercial rabbit anti-NP antibody (Sino Biological) and NP protein standard (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ELISA plates (Evergreen Scientific) were coated with 100 µL (1 µg/mL) per well of SARS-CoV-2 Secto protein (Sino Biological), SARS-CoV-2 Secto-β protein ...
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bioRxiv - Microbiology 2022Quote: ... The supernatants of Expi293™ cells transfected with pAd/SARS-CoV-2-S1SA was diluted 1:40 in PBS-T with 1% BSA and along with standard control protein 40591-V08H (rS1H, Sino Biological) or purified rSARS-CoV-2S1Beta were incubated overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were then fixed and virus-specific foci were detected by antibody against RSV F protein (Sino Biological, 11049-R338) or RSV G protein (Vazyme ...
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Efficacy of recombinant NP-M1 and NP-M1-CRT DNA vaccines against Influenza A viruses in mice C57/BL6bioRxiv - Molecular Biology 2021Quote: ... and then membrane incubated with mouse anti-influenza A virus NP protein monoclonal Ab (Sino biological Company, China; 1: 10,000, 1 h) or mouse anti-influenza polyclonal anti-body (1 ...
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bioRxiv - Biochemistry 2020Quote: ... the “BiEV index (caspase 14)” was used based on the fluorescein-labeled recombinant caspase 14 protein (11856-H07E; Sino Biological, Beijing, China) labeled with NHS-fluorescein (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Prepared elastic Fab anti-Fc-functionalized PAA nanotextures were incubated with 20 μg/mL human ICAM1-Fc chimeric protein (Sino Biological, China) in cold PBS (4°C ...
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bioRxiv - Immunology 2021Quote: ... isolated splenocytes were cultured with 10 mg/ml recombinant SARS-CoV-2 (2019-nCoV) Spike Protein (S1+S2 ECD, His tag) (Sino biological Europe). Recombinant Ovalbumin [10 mg/ml] served as negative protein control ...
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Oral delivery of SARS-CoV-2 DNA vaccines using attenuated Salmonella typhimurium as a carrier in ratbioRxiv - Microbiology 2020Quote: ... then proteins were monitored with ECL Western blotting substrate (Pierce) after incubation with anti-SARS-CoV-2-S-RBD (Sino Biological, China) or anti-β-actin antibody (Solarbio ...
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bioRxiv - Microbiology 2020Quote: SARS-CoV-2 spike specific antibody was generated by immunized Balb/c mouse with SARS-CoV-2 spike protein (RBD, Fc Tag, 40592-V05H, Sino Biological inc). MAbs against SARS-CoV-2 RBD were produced using hybridoma technology and were characterized by pseudotyped virus assay.
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bioRxiv - Microbiology 2020Quote: ... The horses were inoculated subcutaneously with SARS-CoV-2 Spike Protein (S1 Subunit, His tag) (Sino Biological, China, cat no:40591-V08H) antigens containing 1.0mg ...
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bioRxiv - Immunology 2020Quote: ... The expression of SARS-CoV-2 S protein was analyzed by Western blotting with rabbit polyclonal antibody to SARS-CoV-2 RBD (Sino Biological, China) and heat-inactivated human serum samples from Chinese COVID-19 infected patients ...
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bioRxiv - Bioengineering 2021Quote: ... were coated with 100 mM bicarbonate/carbonate buffer (pH 9.6) containing 0.03 μg/mL of recombinant proteins (VP1) from homologous wild-type viruses (Sino Biological Inc., Beijing, China) overnight at 4 °C ...
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bioRxiv - Microbiology 2021Quote: ... Cells were then incubated with a rabbit anti-SARS-CoV nucleocapsid protein polyclonal antibody (Cat. No.: 40143-T62, Sino Biological, Inc. Beijing), followed by an HRP-labelled goat anti-rabbit secondary antibody (Cat ...
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bioRxiv - Immunology 2021Quote: ... Plates were coated with 1μg/ml of SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1), SARS-CoV-2_DB_his (monomeric RBD ...
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bioRxiv - Bioengineering 2023Quote: ... The libraries were subjected to five rounds of biopanning against the recombinant SARS-CoV-2 BA.1 RBD protein (Sino Biological Inc.) as described previously38 ...
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bioRxiv - Microbiology 2022Quote: ... Plasmids expressing the SARS-CoV and SARS-CoV-2 S proteins were purchased from Sino Biologicals (catalog # VG40150-G-N; VG40589-CY). Expression of different coronavirus E proteins ...
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bioRxiv - Immunology 2023Quote: ... 26.4 pmol His-Tagged Biotinylated SARS-CoV-2 (2019-nCoV) spike RBD Recombinant Protein (Sino Biological Inc.; cat number: 40592-VO8H-B) was firstly incubated for 1 hour with 3.78 pmol Streptavidin Alexa Fluor 647 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... The S protein expression from cell lysates was analyzed by Western blot using an anti-SARS-CoV-2 RBD polyclonal antibody (Sino Biological, China) and GAPDH as a loading control.
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bioRxiv - Immunology 2024Quote: ... in PBS at 4°C overnight in absence or presence of recombinant BTN2A2-Fc (10 μg/ml) or Fc tag protein (Cat#10702-HNAH, Sino Biological Inc) or 293 cells derived human BTN2A2-Fc or mouse BTN2A2 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Evaluation of RSV F-specific antibody titers were conducted as previously described.[29] ELISA plates were coated with recombinant human RSV fusion glycoprotein (total-F protein, final concentration: 0.2μg /mL) (Sino Biological Inc. 11049-V0813). The coated plates were incubated with a given serum dilution ...
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bioRxiv - Microbiology 2020Quote: ... recombinant SARS-CoV-2 RBD fusion protein with the Fc region of mouse IgG1 at the C-terminus (RBD-Fc) was purchased from Sino Biological (Beijing, China), recombinant SARS-CoV-2 RBD with a C-terminal His-tag was purchased from Kactus Biosystems (Shanghai ...
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bioRxiv - Immunology 2021Quote: ... Cells infected with MV-014-212 were stained with primary rabbit anti-SARS-CoV-2 spike protein polyclonal antisera (Sino Biologicals, Beijing, China). The plates were incubated for 1 hour at room temperature with constant rocking ...
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bioRxiv - Biochemistry 2020Quote: ... via baculovirus and S protein (S1 and RBD, His tag) expressed in human embryonic kidney cells (HEK293) were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Biochemistry 2020Quote: ... (Cat # 10108-H08H), and S1-His (spike protein residues 1-667, C-terminal His tag) (Cat # 40150-V08B1) were acquired from Sino Biological (Wayne, PA). All proteins were reconstituted in 1X PBS at pH 7.4 supplemented with 0.05 mg/mL bovine serum albumin (BSA) ...
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bioRxiv - Bioengineering 2021Quote: ... was incubated with 5 ng/µl of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-His Recombinant Protein (Sino Biological #40589-V08B1) in phosphate buffer (pH 6.0) ...
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bioRxiv - Microbiology 2020Quote: ... For the detection of SARS-CoV-2 spike protein a rabbit polyclonal anti-SARS-CoV-2 spike S2 antibody (Sino Biological #40590-T62) was used.
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bioRxiv - Immunology 2021Quote: ... Potency was assessed using a cell-based immunosorbent assay to quantify infection by detecting the SARS-CoV-2 nucleoprotein using a specific antibody raised against this protein (Sino Biological, Wayne, PA). The secondary antibody (Life Technologies ...
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bioRxiv - Immunology 2021Quote: ... with 5×105 TCID50 of recombinant MeV-derived vaccine virus in 200 μl volume or subcutaneously (s.c.) with 10 μg recombinant SARS-CoV-2 S protein (Sino Biological Europe, Eschborn, Germany) adjuvanted with 500 μg aluminum hydroxide (Allhydrogel adjuvant 2% ...
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bioRxiv - Immunology 2021Quote: ... and incubated the monolayer with the rabbit polyclonal antibody specific to SARS-CoV-2 RBD protein (1:200) (cat. n° 40592-T62, Sino Biological, Beijing, China), and a chicken antiserum specific to Newcastle disease virus (1:200 ...
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bioRxiv - Immunology 2022Quote: ... pre-incubated beads were incubated a second time with 2 µg of a recombinant human DC-SIGN protein with a human Fc tag (Sino Biological, Köln, Germany) for 1 h at 4°C with agitation ...
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bioRxiv - Microbiology 2021Quote: Biotinylated SARS-CoV-2 Spike D614G protein (Spikebiotin, in-house generated) or the biotinylated SARS-CoV-2 Spike receptor-binding domain (RBDbiotin, Sino Biological, 40592-V08B) were incubated with Alexa Fluor® 647 streptavidin (AF647-strep ...
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bioRxiv - Biochemistry 2020Quote: ... via a baculovirus, and S protein (S1, His tag) expressed by human embryonic kidney (HEK293) cells were purchased from Sino Biological (Beijing, China). Sequencing-grade trypsin and Glu-C were obtained from Enzyme & Spectrum (Beijing ...
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bioRxiv - Immunology 2021Quote: ... 1μg of formalin and heat inactivated SARS-CoV 2 adjuvanted in Alhydrogel (Brenntag) 1% or 1.5 μg of recombinant SARS-CoV-2 spike protein (S1+S2 ECD, His tag; Sino Biological, Cat. 40589-V08B1) adjuvanted in Alhydrogel (Brenntag ...
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bioRxiv - Microbiology 2020Quote: The concentration of the S protein was determined with an ELISA kit (SARS-CoV-2 Spike ELISA kit, Sino Biological Inc., Beijing, China). A monoclonal antibody specific for the S protein of SARS-CoV-2 was pre-coated onto well plates ...
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bioRxiv - Immunology 2023Quote: ... vaccines were formulated in 100 μL of PBS 1X and contained a 10 µg antigen dose of spike S1+S2 ECD (R683A, R685A, F817P, A892P, A899P, A942P, K986P, V987P)-His Recombinant Protein (Sino Biological 40589-V08H4) and a 20 µg 30% CpG-NPs adjuvant dose ...
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bioRxiv - Microbiology 2022Quote: ... spleen and tracheobronchial lymph node were covered with a mouse monoclonal anti-SARS-CoV nucleocapsid protein (#40143-MM05, Sino Biological, Chesterbrook, PA, USA) at a dilution of 1:6000 and incubated at room temperature for forty five minutes ...
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bioRxiv - Microbiology 2022Quote: ... followed by radiolabeling and immunoprecipitation analysis using a mouse monoclonal antibody directed against the HA-tag (Thermo-Fisher, #26183) or an antibody against SARS-CoV Spike protein (Sino Biologicals, #40590-T62).
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bioRxiv - Microbiology 2024Quote: ... They were then probed for protein with a combination of anti-HA monoclonal and polyclonal Abs (Sino Biologicals 11055-RM10 and 11055-T62). IRDye 680RD goat anti-rabbit IgG secondary (LI-COR 926-68071 ...
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bioRxiv - Immunology 2021Quote: ... 100 μg of recombinant SARS-CoV-2 (2019-nCoV) spike S1 protein (Cat: 40591-V08H and 40591-V08H10, Sino Biological, endotoxin level: <0.001U/μg) was used per dose ...