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Citations for World Precision Instruments :
101 - 150 of 192 citations for Vanin 1 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2021Quote: ... FP were excised after topical application of 1% lidocaine using sterile curved spring micro-scissors (McPherson-Vannas, WPI, Sarasota, FL) Type # SR5603 (Roboz Surgical Instrument Co ...
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bioRxiv - Bioengineering 2021Quote: ... of either 2% or 4% SDS solution was infused through the trachea using a programmable syringe pump (flow rate: 6.3 mL.s-1; AL-4000, World Precision Instruments) to generate a thin film of the detergent solution on the luminal surface of the trachea.49,51 To promote de-epithelization ...
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bioRxiv - Bioengineering 2021Quote: ... Mice were anesthetized with 3.5 % isoflurane and 1 µl of AAV suspension was injected transsclerally into the vitreous using a 34G canula (WPI, USA). Animals were sacrificed under anesthesia by cervical dislocation 3 weeks after injection and eyes were harvested in 4 % paraformaldehyd for Immunohistochemistry or liquid nitrogen for quantitative reverse transcriptase-PCR.
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bioRxiv - Microbiology 2022Quote: ... stereotaxic coordinates are shown in Table 1) using a Hamilton syringe connected to a pump (model: UltraMicroPump3; World Precision Instruments). The skin incision on the dorsal head was closed with sterile nylon suture and 2-4% topical chlorhexidine solution was be applied over the closed incision ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Neuroscience 2022Quote: ... and the femoral artery was cannulated to monitor mean arterial blood pressure (MABP; Pressure Monitor BP-1, World Precision Instruments) and for blood gasses sampling and delivery of fluorescent probes ...
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Olfactory receptor neurons generate multiple response motifs, increasing coding space dimensionalitybioRxiv - Neuroscience 2022Quote: ... Odorized air (0.5L min-1) was delivered by injecting air with a pneumatic pump (Reliable Pneumatic PicoPump, World Precision Instruments) into the head space of a 100ml glass bottle containing odorant solutions diluted in mineral oil (JT Baker ...
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bioRxiv - Cancer Biology 2022Quote: ... The Nanoject was mounted at a 25-degree angle relative to the skull surface at each location and actuated by a manual and 1-direction motorized micromanipulator (WPI) at speeds of 600 μm per min ...
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bioRxiv - Biophysics 2023Quote: ... Virus suspensions were slowly injected (100 nl min−1) using a 34 G beveled needle (Nanofil syringe, World Precision Instruments). After injection ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocytes were plated with a density of 50.000 cells/ml on laminin-coated (1:100) fluorodishes (World Precision Instruments, USA) or culture plates ...
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bioRxiv - Neuroscience 2023Quote: ... Recordings were performed with low resistance (1–3 MΩ) glass pipettes (ID: 0.68 mm, OD: 1.2 mm, WPI, Sarasota, FL) filled with aCSF under current-clamp mode with I=0 configuration ...
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bioRxiv - Molecular Biology 2024Quote: ... the brain was immediately removed from the skull and sliced into 1 mm thick coronal brain slices with the help of the coronal brain matrix (WPI). The coronal sections were placed on cold saline-soaked filter paper on an ice bath ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2024Quote: ... in the dPAG at a rate of 0.05 µl/min (total volume of 0.5 µl) via a microinjection pump (UMP3-1, World Precision Instruments) with a 33-gauge syringe (Hamilton) ...
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bioRxiv - Cell Biology 2022Quote: ... 1 mg/mL and then embedded in 0.75% low melting point agarose prepared in AFW in a FluoroDish (World Precision Instruments). Specifically ...
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bioRxiv - Developmental Biology 2019Quote: ... 22hpf embryos were imbedded in 1.1% low gelling agarose in 1-inch glass bottomed Flourodish cell culture dishes (World Precision Instruments) and covered in embryo media ...
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bioRxiv - Neuroscience 2020Quote: ... A small craniotomy was made above the S1-HP (1 mm posterior, 1.5 mm lateral from Bregma) using a dental drill (World Precision Instruments). The stereotaxic coordinates were assessed in a set of control experiments using flavoprotein autofluorescence imaging.
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bioRxiv - Neuroscience 2020Quote: ... connected to an air pressure injector (holding pressure: < 1 PSI, injection pressure: 7 to 10 PSI; PV820 Pneumatic Picopump, WPI, USA) (Zerimech et al. ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2019Quote: ... Recording patch pipettes were pulled with a PC-10 vertical electrode puller (Narishige) from 1.5 mm outer and 1 mm inner diameter patch clamp glass capillaries without filament (WPI, #PG52151-4). Pipette resistance with Ca2+ current recording solutions was ∼3.5 MΩ for pupal and adult MN5 ...
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bioRxiv - Neuroscience 2019Quote: Field EPSPs (fEPSPs) were recorded as previously (19) in CA1 stratum radiatum with glass electrodes (1–3 MΩ; WPI, Saratosa, FL) filled with ASCF ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were embedded in a 1.2% Low-gelling agarose in a 1-inch glass bottom cell culture dish (Fluordish, World Precision Instruments) and visualized using a Nikon C2+ confocal microscope with a 20x (0.95NA ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 1-5 nl mRNAs were microinjected into one-cell stage wild-type zebrafish embryo using the WPI microinjector (WPI, USA). The 1-2 dpf embryos were anesthetized in 0.016% tricaine and photographed under a Leica stereomicroscope.
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bioRxiv - Cell Biology 2019Quote: Glass capillaries with an inner and outer diameter of 1 mm and 0.58 mm respectively (1B100-4 or 1B100F-4, World Precision Instruments) were used to create microneedles ...
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Individual kinetochore-fibers locally dissipate force to maintain robust mammalian spindle structurebioRxiv - Cell Biology 2019Quote: Glass capillaries with an inner and outer diameter of 1 mm and 0.58 mm respectively (1B100-4 and 1B100F-4, World Precision Instruments) were used to create microneedles using a pipette puller (P-87 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2022Quote: ... We injected the construct AAV2-CamKIIa-hChR2(H134R)-mCherry (1 μl, UNC Vector Core) unilaterally using a microsyringe (34 GA. beveled Needle, 10 μ l NANOFIL syringe, WPI) into the left LGN (AP 3.1 ...
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bioRxiv - Neuroscience 2023Quote: ... A total volume of 0,5 ul of the virus was injected at a controlled rate of 0.1 µl min−1 using a microinjector (WPI, Sarasota, USA) The pipette was slowly lowered to the target site and remained in place for another 5 min post-injection before being slowly withdrawn ...
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bioRxiv - Neuroscience 2023Quote: ... Microinjections were performed with a 1 μl Neuros Hamilton syringe (Hamilton, Reno, NV) controlled by a micro-infusion pump (WPI, FL) at a rate of 100 nl/min ...
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bioRxiv - Neuroscience 2023Quote: ... Physiological responses were amplified by 10,000x (80dB) with a 1-Hz to 10-kHz band-pass filter (IsoDAM, WPI, FL, USA), prior to being digitized at 40 kHz and 16-bit using an Analog-to-Digital Converter (NI9205 ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Neuroscience 2023Quote: ... viruses were injected at a rate of 0.1□μl□min−1 with a 10 µl Nanofil syringe (34 GA blunt needle, World Precision Instruments) into the striatum (1.1 mm anterior to bregma ...
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bioRxiv - Biophysics 2022Quote: ... WT and mutant rP2X2R mRNAs (concentration 50 - 3000 ng μl−1) were injected into the oocyte cytoplasm with a Nanoliter 2010 injector (World Precision Instruments). The volume of mRNA injected varied depending on the construct (10-50 nl) ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Neuroscience 2020Quote: ... In some experiments drugs (1 mM Glutamate or 100 μM ATP) were puffed for 250 ms just before the bleaching using Pneumatic PicoPump (World Precision Instruments). Imaging ...
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bioRxiv - Neuroscience 2022Quote: In vivo intracellular recordings of cortical cells were performed in the primary visual cortex (area 1. 17) using sharp Glass electrodes pulled from 1.5-mm-thick borosilicate capillaries (World Precision Instruments Inc.). Electrodes were filled with a 2M potassium methyl sulfate (pH ranging from 7.2 to 7.4) ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2020Quote: ... intracranial pressure (ICP) was monitored via the ventricle cannulation connected to a transducer and a pressure monitor (BP-1, World Precision Instruments). ICP was acquired at 1 kHz ...
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bioRxiv - Physiology 2020Quote: ... The electrodes were insulated by a 3μm kapton sheath (electrode OD 0.356mm) exposing a tip of length 5mm and tip diameter of 1-2μm (World Precision instruments, ref TM33B20KT). They were placed either in the endocardial surface ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Compounds were superfused to the neuron using a syringe pump (New Era Pump Systems Inc, USA) loaded with 1 mL syringe connected to a 50 cm MicroFil 28G (World Precision Instruments) at 2 μL/min allowing rapid solution exchanges.
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bioRxiv - Molecular Biology 2022Quote: ... Action potentials were caused by passing a 1 ms current pulse through platinum-iridium electrodes from an isolated current stimulator (World Precision Instruments). Neurons were perfused in saline-based Tyrode’s buffer consisting of 119 mM NaCl ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized eggs were collected after timed matings of adult zebrafish and injected at the 1-cell stage using a Picopump (World Precision Instruments). Embryos were injected with concentrations ranging from 0.15 – 0.5 mM in a volume of 1 nl.
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bioRxiv - Neuroscience 2019Quote: ... the cranial window contained two ~1 mm diameter holes pre-drilled and filled with a silicone elastomer (Kwik-Sil, World Precision Instruments). A custom 3D-printed headpost was placed around the cranial window and affixed to the skull using C&B Metabond dental cement (Parkell) ...
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bioRxiv - Neuroscience 2019Quote: ... Two catheters were inserted: the first into the left femoral artery to monitor arterial blood pressure (Pressure Monitor BP-1, World Precision Instruments) and inject compounds ...
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bioRxiv - Neuroscience 2019Quote: ... Papaverine was locally ejected for ~1 s by 3 times using an air pressure of <15 psi in the pipette (PV830 Pneumatic PicoPump, World Precision Instruments). A red cloud (Alexa 594 ...
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular field potentials from de CA1 pyramidal neurons were recorded using a borosilicate glass micropipette (1–3 MΩ; RRID:SCR_008593; World Precision Instruments, Sarasota, US) filled with aCSF positioned on the slice surface in the stratum radiatum of CA1 and connected to the headstage of an extracellular recording amplifier (NeuroLog System ...