Labshake search
Citations for World Precision Instruments :
51 - 100 of 104 citations for Tetratricopeptide Repeat Protein 5 TTC5 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2019Quote: Zebrafish embryos staged at 24 hours post-fertilisation (hpf) were manually dechorionated using jeweller’s forceps (Dumont #5, World Precision Instruments), then infected at 28-30 hpf by caudal vein injection of Mm6 ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Biophysics 2020Quote: ... After punching a fluid port through the 5 mm thick Sylgard using 0.75 mm diameter biopsy punches (World Precision Instruments) the PDMS chip was bonded onto glass substrates using an 0.3 mbar ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were anesthetized under hypothermia and 5 pulses of 69 nL were injected into the V1 with a nanoliter injector (WPI). Four days later ...
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bioRxiv - Neuroscience 2021Quote: ... Patch electrodes (2–5 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments ...
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bioRxiv - Biophysics 2021Quote: ... The tip of the pipette was cut to an opening diameter between 1~ 5 μm and bent to ~40° using a microforge (DMF1000, WPI).
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bioRxiv - Biophysics 2019Quote: ... an excess amount of chloride cocktail (2-3 times the final volume of the liquid membrane) was delivered as close as possible to the micro-pipette tip using a 34G microneedle (Quickfil 34G-5, WPI, for which the polyimide coating was stripped from the end) ...
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2022Quote: ... was left in place for 3 and 5 min after the first and second injections (100-nl/min, UMP3 electrical pump, WPI) to prevent backflow of the injected virus solution ...
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Developmental Biology 2020Quote: ... A solution containing 25 ng/µL gRNA and 100 ng/µL Cas9 mRNA or 1000 ng/µL Cas9 protein (Toolgen) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). The insertion and/or deletion (indel ...
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bioRxiv - Developmental Biology 2023Quote: ... A solution containing 25 ng/μL gRNA and 100 ng/μL Cas9 mRNA or 1000 ng/μL Cas9 protein (ToolGen Inc.) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). To establish the foxp1b mutant ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Bioengineering 2021Quote: ... Electrochemical characterizations were performed in a three-electrode configuration, using a thick (2-mm diameter, ~5 mm2) Pt wire (WPI, 99.99%) as counter electrode (CE ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy was sealed with agarose (1.5–2%, dissolved in artificial cerebrospinal fluid) and a 5 mm glass coverslip (World Precision Instruments) was placed over it ...
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bioRxiv - Microbiology 2019Quote: ... They were filled with a 1M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments).
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 1-5 nl mRNAs were microinjected into one-cell stage wild-type zebrafish embryo using the WPI microinjector (WPI, USA). The 1-2 dpf embryos were anesthetized in 0.016% tricaine and photographed under a Leica stereomicroscope.
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Neuroscience 2021Quote: ... was injected into PR at 0.5 µl/min using G33 needle (Hamilton) connected to a 10 µl micro-syringe controlled by a micro-pump (WPI, UltraMicroPump 3). AM cells were recorded both before and 30 min after injection.
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated as single cells (5 × 105 cells) on 35 mm FluoroDish Cell Culture Dishes (World Precision Instruments, cat. WD35-100) and allowed to attach for 72 h ...
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bioRxiv - Physiology 2019Quote: ... TEER values were determined on days-2 and −5 with an Epithelial Volt ohm meter 2 (EVOM2) and STX2 series chopstick electrodes (World Precision Instruments).
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bioRxiv - Neuroscience 2019Quote: ... Anesthesia was induced at 5% isofluorane in O2/N2O mixture (Rothacher Medical GmbH., Switzerland) and mice were placed in the stereotaxic frame (World Precision Instruments). During surgery ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2020Quote: Electrical stimulation was applied through ACSF filled glass pipette (tip diameter 3–5 µm) connected to a linear stimulus isolator (A395, World Precision Instruments) was used to deliver monopolar electrical stimulation (10–90 µA ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Biophysics 2024Quote: ... A Polydimethylsiloxan (PDMS) stencil with a circle area of 5 [mm] was placed inside a bottom glass dish (FluoroDish, World Precision Instruments), which is a 35 [mm] diameter glass bottom dish ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Neuroscience 2019Quote: Eight to eleven weeks old injected mice were anesthetized with 5% of isoflurane a 1.5% of oxygen into the anesthesia system box (World Precision Instruments, Ref. EZ-B800) for 5 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... and grown with daily media changes until the transepithelial resistance reached 400 ohm/cm2 (4–5 days) measured with an EVOM electrometer (World Precision Instruments, Sarasota, FL).
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bioRxiv - Neuroscience 2020Quote: Extracellular single-unit recordings confined to superficial cerebellar cortical layers were obtained in the awake animal during the reaching task using either parylene-insulated tungsten microelectrodes (WPI, impedance 2-5 MΩ) or a 16 channel Vector Array microelectrode (Neuronexus ...
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bioRxiv - Physiology 2021Quote: The mixed sample was then transferred to a 5 mL syringe and perfused using an Aladdin AL-1000 syringe pump (World precision instruments, United Kingdom) through the ibidi slide ...
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bioRxiv - Cell Biology 2023Quote: ... The micropipette was bent to a desired degree by heating it over an alcohol lamp and was filled with phosphate-buffered saline (PBS) using the MicroFil (World Precision Instruments, MF34G-5). The micropipette was installed to a micropipette holder ...