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Citations for World Precision Instruments :
151 - 197 of 197 citations for Stem Cell Culture Media since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Pathology 2019Quote: iPSC imaging assays were set up a day before by seeding cells onto Geltrex coated 35mm Fluro dishes (WPI). Prior to staining ...
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bioRxiv - Developmental Biology 2020Quote: ... One-cell-stage AB WT embryos were injected with gRNA and Ca9 mRNA with a Picopump (World Precision Instruments). Potential founders (F0 ...
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bioRxiv - Neuroscience 2020Quote: ... and anterior muscle cells were patched using fire-polished 4−6 MΩ resistant borosilicate pipettes (World Precision Instruments, USA). Membrane currents were recorded in the whole-cell configuration by HEKA EPC-9 patch clamp amplifier ...
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were obtained from NAc shell MSNs using borosilicate glass pipettes (3-5 ΩM; WPI) filled with intracellular solution containing (in mM) ...
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bioRxiv - Microbiology 2023Quote: ... The coverslip-pad-cell sample assembly was placed inverted onto the base of a 35 mm #1.5 FluoroDish (WPI)45 ...
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bioRxiv - Microbiology 2023Quote: ... expressing C91-PL cells were diluted in TNE 1X (Tris/HCl pH7.4 NaCl, EDTA) and plated on glass-bottom fluorodishes (WPI) coated with 0.01% poly-L-lysine (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cells were plated at 20,000 per cm2 on 35 mm dishes (World Precision Instruments, Inc, #FD 35-100) pre-coated with collagen I (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2020Quote: ... A solution containing 25 ng/µL gRNA and 100 ng/µL Cas9 mRNA or 1000 ng/µL Cas9 protein (Toolgen) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). The insertion and/or deletion (indel ...
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bioRxiv - Cancer Biology 2019Quote: CRISPR against vhl and AR was injected into the newly fertilized 1-cells stage embryos using microinjector (Pneumatic PicoPump WPI) at 100uM concentration at 1nl volume.
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bioRxiv - Neuroscience 2021Quote: ... Motoneurons were visualized and whole cell recordings obtained under DIC illumination with pipettes (L: 100 mm, OD: 1.5 mm, ID: 0.84 mm; World Precision Instruments) pulled on a Flaming Brown micropipette puller (Sutter instruments P97 ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch clamp recordings were obtained with glass pipette electrodes pulled from borosilicate capillaries (World Precision Instruments, Sarasota, FL) on a P97 Flame/Brown Puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... Motoneurons were visualized and whole-cell recordings obtained under DIC using pipettes (L: 100 mm, OD: 1.5 mm, ID: 0.84 mm; World Precision Instruments) pulled on a Flaming Brown micropipette puller (Sutter instruments P97 ...
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bioRxiv - Cell Biology 2023Quote: ... cells were plated in 35 mm petri dishes containing 23 mm #1.5 poly-D- lysine-coated coverslips (World Precision Instruments) two days prior to imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... Cardiomyocytes were plated with a density of 50.000 cells/ml on laminin-coated (1:100) fluorodishes (World Precision Instruments, USA) or culture plates ...
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Developmental Biology 2023Quote: ... A solution containing 25 ng/μL gRNA and 100 ng/μL Cas9 mRNA or 1000 ng/μL Cas9 protein (ToolGen Inc.) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). To establish the foxp1b mutant ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Cancer Biology 2023Quote: ... 300-500 cancer cells were injected into duct of cuvier (DoC) of ZF embryos using a Pneumatic Picopump and a manipulator (WPI). The injected embryos were further maintained in a 34 °C incubator ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipette electrodes with a tip resistance between 10 and 14 MΩ for cones and 6-8 for horizontal cells were pulled from thick-walled borosilicate glass (1.5 mm outer diameter; 0.84 mm inner diameter; World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were obtained using patch pipettes pulled from borosilicate glass capillaries (1B120F-4, World Precision Instruments). Pipette tip resistances were ∼4-10 MΩ for amacrine cell recordings in the ganglion cell layer ...
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Neuroscience 2023Quote: ... and muscle cells were patched using fire-polished 4–6 MΩ resistant borosilicate pipettes (1B100F-4, World Precision Instruments, USA). Membrane currents were recorded in the whole-cell configuration by PULSE software with a HEKA EPC-9 amplifier (Germany) ...
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bioRxiv - Microbiology 2019Quote: ... About 20.000 or 150.000 cells were seeded in 8-well chamber slides (see above) or 3.5 mm glass bottom dishes (FluoroDish, WPI, Berlin, Germany), respectively ...
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bioRxiv - Developmental Biology 2019Quote: ... A total of 1-5 nl mRNAs were microinjected into one-cell stage wild-type zebrafish embryo using the WPI microinjector (WPI, USA). The 1-2 dpf embryos were anesthetized in 0.016% tricaine and photographed under a Leica stereomicroscope.
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Cell Biology 2023Quote: Barrier integrity of cell monolayers was assessed via TEER using an Epithelial Volt/Ohm Meter (World Precision Instruments, EVOM2 or EVOM3) and STX100C96 electrode for 96-well cultures or STX2 electrode for 12-well cultures ...
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bioRxiv - Developmental Biology 2024Quote: Control and SLAP overexpressing HEK cells were seeded 24 h before mechanical probing by AFM onto glass bottom dishes (WPI, Fluorodish). Thirty min before starting the AFM indentation tests ...
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bioRxiv - Neuroscience 2020Quote: Somatic whole cell patch clamp recordings of visually identified CA1 principal cells were performed after 16-23 DIV with microelectrodes (3-8 MΩ) prepared from borosilicate glass capillaries (1B150F-4 World Precision Instruments) using a Sutter P-1000 puller ...
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bioRxiv - Cell Biology 2021Quote: ... 0.075 × 106 FHIP2A KO U2OS cells stably expressing FHIP2A-TagRFPT-V5 were plated into a 35mm glass bottom FluoroDish with 23mm well (World Precision Instruments) and grown for 24 hours ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... the cells were approached with micropipettes of 1.2-3.3 MΩ resistance manufactured from 1.5 mm OD glass (World Precision Instruments, Sarasota, Florida, USA) on a microelectrode puller ...
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bioRxiv - Neuroscience 2019Quote: ... TdTomato-expressing (Vglut3lineage) dissociated DRG neurons and HEK cells were performed with patch pipettes pulled from standard borosilicate glass (1B150F-4, World Precision Instruments) with a P-97 puller (Sutter Instruments) ...
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bioRxiv - Immunology 2019Quote: ... Embryos were collected at the one cell stage and injected using non-filament glass capillary needles (Kwik-FilTM Borosilicate Glass Capillaries, World Precision Instruments (WPI), Herts ...
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bioRxiv - Developmental Biology 2020Quote: ... Fertilized eggs were collected after timed matings of adult zebrafish and injected at the 1-cell stage using a Picopump (World Precision Instruments). Embryos were injected with concentrations ranging from 0.15 – 0.5 mM in a volume of 1 nl.
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Developmental Biology 2023Quote: ... One-cell stage fertilized eggs were aligned in a custom-made setup and microinjected into the cell using pressurized microinjector from World Precision Instruments. Injection volumes were optically adjusted to about 5% from the cell volume ...
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bioRxiv - Biophysics 2023Quote: ... ∼0.5 ml of prewarmed antibiotic-free complete media was added to the electroporation cuvette and the cell suspension was transferred to 35 mm poly-D-lysine coated FluoroDish optical dishes (WPI, #FD35PDL-100) for a final volume of 2 ml of antibiotic-free complete media for test imaging ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended and 45 µL of cells were added to 955 µL of DB and placed in a 35 mm glass bottom dish (FD35-100, World Precision Instruments) for imaging.
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bioRxiv - Biophysics 2022Quote: ... One day before the experiment the cells were transferred to a 35 mm glass-bottom Petri dish (FluoroDish, World Precision Instruments Germany GmbH). 30min prior to the measurements the culture medium was exchanged to Leibovitz’s L-15 medium without phenol red (21083027 ...
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bioRxiv - Microbiology 2021Quote: ... and urea were measured using a high-sensitivity colorimetric approach with an AutoAnalyzer II (Technicon) and Liquid Waveguide Capillary Cells (World Precision Instruments, USA) as outlined (51) ...
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bioRxiv - Immunology 2019Quote: ... Embryos were collected at the one cell stage and injected using non-filament glass capillary needles (Kwik-FilTM Borosilicate Glass Capillaries, World Precision Instruments (WPI), Herts ...
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bioRxiv - Immunology 2019Quote: ... Embryos were collected at the one cell stage and injected using non-filament glass capillary needles (Kwik-Fil™ Borosilicate Glass Capillaries, World Precision Instruments (WPI), Herts ...
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bioRxiv - Cell Biology 2022Quote: ... For experiments conducted with healthy donors for the CBF and cell velocity the bronchial epithelial barrier function was evaluated by quantifying TEER using an EVOM epithelial voltohmmeter (World Precision Instruments, FL, USA) connected with the STX2 electrodes.
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Immunology 2019Quote: ... Embryos were collected at the one cell stage and injected using non-filament glass capillary needles (Kwik-Fil™ Borosilicate Glass Capillaries, World Precision Instruments (WPI), Herts ...
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bioRxiv - Microbiology 2022Quote: ... Cilia activity was checked every 2~3 days and trans-epithelial electrical resistance (TEER) was measured to ensure confluency of the cells using the EVOM meter (World Precision Instruments, Sarasota, FL).
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bioRxiv - Physiology 2023Quote: ... Cell monolayer transepithelial voltage (VTE) and resistance (RTE) were measured using an epithelial volt-ohmmeter equipped with stick electrodes (World Precision Instruments, Sarasota, FL). The equivalent transepithelial current (ITE ...
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bioRxiv - Neuroscience 2020Quote: ... then a small volume of agonist-containing solution was puffed on the cells via a pneumatic pico-pump (PV820; World Precision Instruments, Inc., Sarasota, FL) with a brief pressure (10 psi ...