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Citations for World Precision Instruments :
101 - 142 of 142 citations for SARS CoV 2 Spike Glycoprotein S2 Sheep Fc Tag HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Neuroscience 2023Quote: ... Glass pipettes with a resistance of 2-4 MΩ were pulled from borosilicate glass capillaries (World Precision Instruments, Cat# TW150-4) using a PC-10 pipette puller (Narishige) ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with 3% isoflurane (Aerrane, Henry Schein) for 2 min and then transferred to the stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% and monitored the same way as described above for IHKA injection ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2021Quote: ... was inserted in the dorsal horn to carefully deliver CAV virus at 2 nl/s with the aid of electronically controlled nanoinjector (Nanoliter 2010, WPI, FL, US). Three injections ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 min in a rectangular transparent plexiglass box (induction chamber) and then transferred to a stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% ...
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bioRxiv - Microbiology 2021Quote: ... The dorsal side of their ears was then either exposed to mosquito bites for two minutes or injected with 0.2 μL of sporozoite suspension using a micro syringe (NanoFil 10 μL syringe mounted with a 35G beveled needle, World Precision Instruments), yielding fields of view containing in average 100 parasites ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nl of one of the viral vectors listed above at a titer of 2*1012 particles/ml was injected per site (PMd, amv dlPAG) using a 10 μl nanofil syringe (World Precision Instruments) at 0.08 μl/min ...
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bioRxiv - Neuroscience 2021Quote: ... we used four individual samples (exceptions: SST RML 10 WPI: n = 3; PV NBH 10 WPI: n = 3; Astro RML 18 WPI: n = 2). RNA was converted to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Aplied Science) ...
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 1 µl (2 x 1010 GC/hemisphere) virus was dispensed using a NanoFil syringe with a 33G beveled needle (World Precision Instruments). The other hemisphere was injected in the same way ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings from GPe neurons were obtained using micropipettes (2–4MΩ) made from 1.5 mm OD/1.12 mm ID borosilicate glass with a filament (World Precision Instruments, Sarasota, FL) pulled with a P-97 puller (Sutter Instruments Novato ...
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bioRxiv - Neuroscience 2023Quote: ... Three circular glass coverslip (2 of 3 mm and one 4mm coverslips) was affixed with a clear silicone elastomer (Kwik-sil, World Precision Instruments). A custom 3D-printed stainless steel headpost was attached on the skull and the rest of exposed skull area and the edge of the cranial window were covered using a dental acrylic (C&B Metabond) ...
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bioRxiv - Neuroscience 2023Quote: ... 20 nl of the Cre-dependent helper virus was injected to the HDB (bilateral, n = 2; unilateral, n = 1) using a Nanoliter 2010 microinjection pump (World Precision Instruments). The injection coordinates were defined by a stereotaxic atlas (Paxinos and Franklin ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
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bioRxiv - Neuroscience 2023Quote: ... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
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bioRxiv - Cell Biology 2024Quote: ... and injected with 3 μl of AAV9-hSyn-iCre (titer 1 x 1013 to 2 x 1014) into the right lateral ventricle using a nanoliter injector (World Precision Instruments; Micro4 MicroSyringe Pump Controller connected with Nanoliter 2000 ...
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bioRxiv - Neuroscience 2020Quote: ... with a 33-G beveled needle that was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, FL, USA). Other surgical procedures and viral injection was as described previously (15).
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bioRxiv - Neuroscience 2020Quote: ... at a rate of 0.2 μL/min with a glass micropipette connected to a Hamilton syringe controlled by stereotaxic syringe holder (SYS-Micro4, WPI, Sarasota, FL, USA). The needle was removed 10 min later ...
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bioRxiv - Molecular Biology 2023Quote: The trans-epithelial electric resistance (TEER) was tracked using the EVOM voltohmmeter equipped with STX-2 chopstick electrodes (World Precision Instruments, Stevenage, UK) during wet (days −3 ...
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bioRxiv - Neuroscience 2023Quote: ... fluoxetine was intracranially delivered with a volume of 58.7 nl/side over 2 min using a motorized single pressure syringe pump (World Precision Instruments, Sarasota, FL). After infusion ...
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bioRxiv - Cancer Biology 2023Quote: ... a custom-made EEG adapter with attached wires (2 recording channels, one reference, each made from Teflon-coated silver (WPI, Sarasota, FL, USA) wire with ∼0.5mm tips exposed ...
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bioRxiv - Bioengineering 2023Quote: ... Pupils were dilated and sub-retinal injections of 2 µL were performed using a Nanofil syringe with a 28-gauge blunt needle (World Precision Instruments, Inc.) under an operating microscope (Leica Microsystems ...
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bioRxiv - Neuroscience 2021Quote: ... we used four individual samples (exceptions: SST RML 10 WPI: n = 3; PV NBH 10 WPI: n = 3; Astro RML 18 WPI: n = 2). RNA was converted to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Aplied Science) ...
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bioRxiv - Cell Biology 2021Quote: ... Transendothelial electrical resistance (TER) was measured daily using an EVOM volt ohm meter equipped with STX-2 electrodes (World Precision Instruments, Sarasota, FL, USA) and electrical resistance calculated as previously described (Bartosova et al. ...
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bioRxiv - Cell Biology 2020Quote: ... The resistance between the upper and lower surfaces of IEC monolayers was measured before (Day 4) and after 2 days of stimulation (Day 6) using an EVOM2 epithelial Volt/Ohm meter (World Precision Instruments, FL, USA). The resistance of IEC monolayers was corrected by subtracting the resistance of scaffolds without cells and normalized by multiplying the effective scaffold surface area to provide a trans-epithelial electrical resistance (TEER ...
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Validating Minimally Invasive Laser Doppler Flowmetry for Serial Bone Perfusion Measurements in MicebioRxiv - Bioengineering 2019Quote: ... was placed directly on the exposed bone surface (Fig. 2) and held in place using a micromanipulator (MM3-ALL, World Precision Instruments, Sarasota, FL) to reduce signal noise from probe movement ...
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bioRxiv - Neuroscience 2020Quote: Extracellular single-unit recordings confined to superficial cerebellar cortical layers were obtained in the awake animal during the reaching task using either parylene-insulated tungsten microelectrodes (WPI, impedance 2-5 MΩ) or a 16 channel Vector Array microelectrode (Neuronexus ...
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bioRxiv - Neuroscience 2021Quote: ... The pipette was filled from the back end with mineral oil and attached to a syringe needle mounted in a microinjection syringe pump (World Precision Instruments, UMP3T-2). Before injection ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subject to nano-electrospray ionization (nESI) in positive ion mode (at ∼2 kV) with a pulled nanospray tip (World Precision Instruments 1B100-3) prepared as detailed in (48) ...
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bioRxiv - Neuroscience 2022Quote: ... with a Gamry 600+ potentiostat (Gamry Instruments, Warminster, PA) (N=2 arrays) using previously published methods89.Silver ground and reference wires (AGT05100, World Precision Instruments, Sarasota, FL) were soldered to the PCB ...
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bioRxiv - Neuroscience 2023Quote: ... the vagus nerve was mechanically stabilized on the micro-cuff electrode by applying a drop of 2-component silicone (Kwik-Sil, World Precision Instruments, Sarasota, FL) and then sectioned either caudal or rostral to the cuff ...
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bioRxiv - Neuroscience 2023Quote: ... Infusions were made at a rate of 100 nL/min utilizing 29 Ga microinjection needles connected to FEP tubing secured to a 2 µl Hamilton syringe and a microinjection pump (UMP3, World Precision Instruments, Sarasota, FL). The infusion system was filled with distilled water and separated from the infused virus or drug by a small air bubble ...
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bioRxiv - Neuroscience 2024Quote: ... the stock solution was further diluted to 0.6 mM with saline and 2 µl of the diluted solution was intracerebroventricularly administered after the control recording sessions using a NANOFIL micro-syringe (World Precision Instruments; FL, U.S.A.) and ultra-micro pomp (UMP3T-1 ...
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bioRxiv - Microbiology 2022Quote: Consumption of O2 was measured using an ISO-OXY-2 O2 sensor attached to an APOLLO 4000 free radical analyzer (World Precision Instruments, Inc., Sarasota, FL) as described52 ...
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bioRxiv - Neuroscience 2019Quote: ... The muscle temporalis was detached from the skull and a 4×2 mm craniotomy was performed using a dental drill (World Precision Instruments, Omnidrill3, tip #7), following the contour delimited rostral and ventrally by the squamosal suture ...
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bioRxiv - Neuroscience 2022Quote: Whole cell patch clamp was performed on interneurons from the lumbar segments using 2–4 MΩ glass electrodes pulled from glass capillary tubes (Item #TW150F-4, World Precision Instruments, Sarasota, FL, USA) with a Flaming-Brown P-97 (Sutter Instrument Company ...
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bioRxiv - Neuroscience 2023Quote: ... The space between the edges of the foil and the head capsule of the fly was sealed with 2-component silicon (Kwik-Sil™, World Precision Instruments, Sarasota, USA). A droplet of Ringer’s solution (NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The virus (AAV1/2-OXTp-hM4Dgi-mCherry or AAV1/2-OXTp-Venus or AAV1/2-OXTp-mCherry) was loaded into a 20μl NanoFil syringe fitted with a 33gauge needle (cat no. NF33BL, World Precision Instruments Inc, Sarasota, FL, USA). A final volume of 270nl was injected into the PVH (A-P −1.7mm ...
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bioRxiv - Biophysics 2021Quote: ... to block or enhance a specific pathway the ear surface was gently cleaned and dried and a 4 µl solution containing a selected drug (Table 2) diluted in a PBS was microinjected in the earlobe skin using a 10 µl NANOFIL syringe (World Precision Instruments Inc., Sarasota, FL, USA) equipped with a gauge 33 needle ...