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Citations for World Precision Instruments :
101 - 150 of 172 citations for Ig lambda constant 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
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bioRxiv - Neuroscience 2023Quote: ... electrodes (2-3 MΩ tip resistance) were pulled from cleaned borosilicate glass capillaries (TW150F-4, World Precision Instruments), their tips were silanized using 5% dichlorodimethylsilane at 220 °C for 30 min and stored in a desiccator ...
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bioRxiv - Cell Biology 2020Quote: ... on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100, WPI). 8 hr following transfection ...
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bioRxiv - Immunology 2021Quote: ... Barrier integrity of ALI cultures was monitored by measuring trans-epithelial electrical resistance (TEER, EVOM 2, World Precision Instruments). ALI cultures were validated for use in experiments based on microscopic appearance of appropriate ciliated morphology and TEER > 500 Ω*cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... TEER values were determined daily beginning on day-2 using an epithelial volt/ohm meter (EVOM2, World Precision Instruments) and STX2 series chopstick electrodes as described previously (23).
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bioRxiv - Neuroscience 2023Quote: ... Item# WE30030.5A5) with 2-3 µm diameter and 0.5 MΩ impedance connected to DAM50 Extracellular Amplifier (World Precision Instruments); data were acquired with Clampex 10.7 (Molecular Devices) ...
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bioRxiv - Neuroscience 2024Quote: ... For all surgical procedures mice were anesthetized using 1.5-2% isoflurane and were injected using a Kopf stereotaxic and World Precision Instruments (WPI) microinjection syringe pump with a 10 mL syringe and 33g blunt needle at 50-100 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2020Quote: ... Patch electrodes (2-4 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments Company ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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bioRxiv - Neuroscience 2021Quote: ... Patch electrodes (2–5 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch-clamp recordings were made using electrodes (2-6MΩ) pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments). They were filled with a pipette solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... and injected with a volume of 2 μL at a rate of 20 nL/s (Micro4, World Precision Instruments, USA) into the vitreous cavity (51730D ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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bioRxiv - Neuroscience 2024Quote: ... we applied 1-s pressure pulses (0.4-0.6 psi) every 2 s for 1 min by a microinjector (PV850, World Precision Instruments). In some experiments ...
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bioRxiv - Microbiology 2019Quote: ... and sporozoites were injected intradermally into the ear pinna in a total volume of 0.2 μl using a NanoFil-10 μl syringe with a NF33BV-2 needle (World Precision Instruments). All sporozoite injections were completed within 30 min of salivary gland dissection to ensure that imaging and parasite conditions remained constant ...
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bioRxiv - Microbiology 2019Quote: ... falciparum sporozoites were then injected into the dermis in a total volume of 0.2 μl using a NanoFil-10 μl syringe with a NF33BV-2 needle (World Precision Instruments). The human skin was then moistened with RPMI medium ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...
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bioRxiv - Bioengineering 2021Quote: ... Electrochemical characterizations were performed in a three-electrode configuration, using a thick (2-mm diameter, ~5 mm2) Pt wire (WPI, 99.99%) as counter electrode (CE ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted lateral-side up on a thin layer of 1.5-2% low-melt agarose in a tissue culture dish with a cover-glass bottom (World Precision Instruments) and covered in E3 media ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy was sealed with agarose (1.5–2%, dissolved in artificial cerebrospinal fluid) and a 5 mm glass coverslip (World Precision Instruments) was placed over it ...
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bioRxiv - Neuroscience 2021Quote: ... A long tempered small tip (2 — 3 μm in diameter) patch pipette (∼3 MΩ; borosilicate glass, WPI, GBF 150-86-10) was used to pressure inject the dye into the extracellular space at 4 — 6 psi for 14 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... VP neurons were visualized using an Olympus BX51WI microscope and recorded from using glass pipettes (1.3-2 MΩ, World Precision Instruments) filled with internal solution (in mM ...
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bioRxiv - Bioengineering 2022Quote: ... a 2-mm diameter craniotomy was then made over the fiducial mark and covered with KwikCast (World Precision Instruments, FL, USA). The mouse then recovered overnight before the recording session.
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bioRxiv - Cell Biology 2022Quote: ... TEER was measured with was monitored daily using an epithelial tissue volt ohmmeter (EVOMX) with STX-2 chopsticks (World Precision Instruments). TEER measurements were calculated in ohms cm2 after subtracting the blank value for the membrane insert ...
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bioRxiv - Neuroscience 2023Quote: ... 1474) and a 75-200 nL volume of viral vector was injected using a microinjector pump (World Precision Instruments, UMP3T-2) with an attached 10-μL NanoFil syringe (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Neuroscience 2023Quote: ... Glass pipettes with a resistance of 2-4 MΩ were pulled from borosilicate glass capillaries (World Precision Instruments, Cat# TW150-4) using a PC-10 pipette puller (Narishige) ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with 3% isoflurane (Aerrane, Henry Schein) for 2 min and then transferred to the stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% and monitored the same way as described above for IHKA injection ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2021Quote: ... was inserted in the dorsal horn to carefully deliver CAV virus at 2 nl/s with the aid of electronically controlled nanoinjector (Nanoliter 2010, WPI, FL, US). Three injections ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 min in a rectangular transparent plexiglass box (induction chamber) and then transferred to a stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% ...
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bioRxiv - Microbiology 2021Quote: ... The dorsal side of their ears was then either exposed to mosquito bites for two minutes or injected with 0.2 μL of sporozoite suspension using a micro syringe (NanoFil 10 μL syringe mounted with a 35G beveled needle, World Precision Instruments), yielding fields of view containing in average 100 parasites ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nl of one of the viral vectors listed above at a titer of 2*1012 particles/ml was injected per site (PMd, amv dlPAG) using a 10 μl nanofil syringe (World Precision Instruments) at 0.08 μl/min ...
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bioRxiv - Neuroscience 2021Quote: ... we used four individual samples (exceptions: SST RML 10 WPI: n = 3; PV NBH 10 WPI: n = 3; Astro RML 18 WPI: n = 2). RNA was converted to cDNA using the Transcriptor High Fidelity cDNA Synthesis Kit (Roche Aplied Science) ...
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 1 µl (2 x 1010 GC/hemisphere) virus was dispensed using a NanoFil syringe with a 33G beveled needle (World Precision Instruments). The other hemisphere was injected in the same way ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings from GPe neurons were obtained using micropipettes (2–4MΩ) made from 1.5 mm OD/1.12 mm ID borosilicate glass with a filament (World Precision Instruments, Sarasota, FL) pulled with a P-97 puller (Sutter Instruments Novato ...
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bioRxiv - Neuroscience 2023Quote: ... Three circular glass coverslip (2 of 3 mm and one 4mm coverslips) was affixed with a clear silicone elastomer (Kwik-sil, World Precision Instruments). A custom 3D-printed stainless steel headpost was attached on the skull and the rest of exposed skull area and the edge of the cranial window were covered using a dental acrylic (C&B Metabond) ...
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bioRxiv - Neuroscience 2023Quote: ... 20 nl of the Cre-dependent helper virus was injected to the HDB (bilateral, n = 2; unilateral, n = 1) using a Nanoliter 2010 microinjection pump (World Precision Instruments). The injection coordinates were defined by a stereotaxic atlas (Paxinos and Franklin ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
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bioRxiv - Cell Biology 2024Quote: ... and injected with 3 μl of AAV9-hSyn-iCre (titer 1 x 1013 to 2 x 1014) into the right lateral ventricle using a nanoliter injector (World Precision Instruments; Micro4 MicroSyringe Pump Controller connected with Nanoliter 2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
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bioRxiv - Neuroscience 2020Quote: ... with a 33-G beveled needle that was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, FL, USA). Other surgical procedures and viral injection was as described previously (15).