Labshake search
Citations for World Precision Instruments :
201 - 249 of 249 citations for CTLA 4 Mouse HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... was used to create a micropipette (tip diameter 30-50 µm) from a micro glass tube (1.0-mm diameter; TW100F-4; WPI, USA). The pulled micropipette was then beveled using a micro grinder (EG-402 ...
-
bioRxiv - Neuroscience 2023Quote: ... Patch pipettes were pulled to resistances of ∼3 MΩ from thin-walled glass capillaries (World Precision Instruments, Cat# 1B120F-4). Importantly ...
-
bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were obtained using patch pipettes pulled from borosilicate glass capillaries (1B120F-4, World Precision Instruments). Pipette tip resistances were ∼4-10 MΩ for amacrine cell recordings in the ganglion cell layer ...
-
bioRxiv - Pathology 2021Quote: ... body temperatures were measured using a thermocouple thermometer and mouse rectal probe (World Precision Instruments, RET-3). Blood glucose measurements were obtained with 2uL of tail vein blood analyzed with a Bayer CONTOUR Blood Glucose Monitor and Test Strips ...
-
bioRxiv - Neuroscience 2020Quote: ... Viral construct were administered in 4 injections within GPe using a UMP3 microsyringe pump injector (World Precision Instruments, Sarasota, FL, USA) with 100µl Hamilton syringe ...
-
bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a resistance of 4–6 MΩ were pulled from borosilicate glass capillaries (1.5 mm outer diameter, 0.86 mm internal diameter, World Precision Instruments, USA) using a micropipette puller (PC-10 ...
-
bioRxiv - Neuroscience 2019Quote: ... Recording patch pipettes were pulled with a PC-10 vertical electrode puller (Narishige) from 1.5 mm outer and 1 mm inner diameter patch clamp glass capillaries without filament (WPI, #PG52151-4). Pipette resistance with Ca2+ current recording solutions was ∼3.5 MΩ for pupal and adult MN5 ...
-
bioRxiv - Neuroscience 2019Quote: Single-unit recordings in 3 awake Pcp2-Cre-Ai27D mice were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1- to 2-μm tips and 3 to 12 MΩ impedance ...
-
bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
-
bioRxiv - Neuroscience 2022Quote: Single-unit recordings of Purkinje neurons and cerebellar nuclei neurons were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1-to 2-μm tips ...
-
bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 0.5 and 4 MΩ using a MF-200 microforge (World Precision Instruments). 90% series resistance (Rs ...
-
bioRxiv - Neuroscience 2024Quote: ... dorsoventral from the brain surface [DV] 1.9 mm) at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For the optogenetic silencing experiments (Figure 1H–N) ...
-
bioRxiv - Neuroscience 2020Quote: ... the mouse was either perfused for histology or its skull was protected with Kwik-Cast (World Precision Instruments) for the next experiment ...
-
bioRxiv - Neuroscience 2020Quote: Somatic whole cell patch clamp recordings of visually identified CA1 principal cells were performed after 16-23 DIV with microelectrodes (3-8 MΩ) prepared from borosilicate glass capillaries (1B150F-4 World Precision Instruments) using a Sutter P-1000 puller ...
-
bioRxiv - Cancer Biology 2019Quote: Melanoma cells were injected into the circulation of 2dpf larvae with a microinjection apparatus and needles were pulled from capillaries (TW100-4, World precision instruments). Transplanted larvae were kept overnight at 28ºC in embryo media ...
-
bioRxiv - Neuroscience 2020Quote: ... The administration speed was held constant at 800 nL/min by an injection pump (World Precision Instruments, Inc., UMP 3-4, USA), and the time interval between each injection path was 5 min (43) ...
-
bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
-
bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
-
bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
-
bioRxiv - Neuroscience 2019Quote: ... TdTomato-expressing (Vglut3lineage) dissociated DRG neurons and HEK cells were performed with patch pipettes pulled from standard borosilicate glass (1B150F-4, World Precision Instruments) with a P-97 puller (Sutter Instruments) ...
-
bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
-
Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
-
bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
-
bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
-
The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
-
bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
-
bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
-
bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
-
bioRxiv - Molecular Biology 2024Quote: ... PRV-CAG-EGFP (BrainVTA, Cat. P03001) was injected into unilateral inguinal WAT (4 × 200 nL) using a 36-gauge beveled needle (WPI, Cat. NF36BV). After the injection ...
-
bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
-
bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
-
bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
-
bioRxiv - Cell Biology 2021Quote: ... and grown with daily media changes until the transepithelial resistance reached 400 ohm/cm2 (4–5 days) measured with an EVOM electrometer (World Precision Instruments, Sarasota, FL).
-
bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
-
bioRxiv - Cell Biology 2020Quote: ... The resistance between the upper and lower surfaces of IEC monolayers was measured before (Day 4) and after 2 days of stimulation (Day 6) using an EVOM2 epithelial Volt/Ohm meter (World Precision Instruments, FL, USA). The resistance of IEC monolayers was corrected by subtracting the resistance of scaffolds without cells and normalized by multiplying the effective scaffold surface area to provide a trans-epithelial electrical resistance (TEER ...
-
bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
-
bioRxiv - Genetics 2019Quote: ... repair templates and nuclease-free water were added to the mixtures and loaded into pulled injection needles (1B100F-4, World Precision Instruments, Sarasota, FL). Individual injected P0 animals were transferred to new 6 cm NGM plates approximately 18 hours after injections ...
-
bioRxiv - Neuroscience 2023Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
-
bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
-
bioRxiv - Neuroscience 2019Quote: ... The muscle temporalis was detached from the skull and a 4×2 mm craniotomy was performed using a dental drill (World Precision Instruments, Omnidrill3, tip #7), following the contour delimited rostral and ventrally by the squamosal suture ...
-
bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
-
bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
-
bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
-
bioRxiv - Pathology 2022Quote: ... The core body temperature was measured rectally with a Thermometer equipped with a RET-3 mouse rectal temperature probe (WPI, Sarasota, FL).
-
bioRxiv - Neuroscience 2021Quote: ... Extracellular recordings were obtained using tungsten electrodes (TM31C40KT, 4-MΩ impedance at 1 kHz or TM31A50KT, 5-MΩ impedance at 1 kHz; World Precision Instruments Inc., Sarasota FL, USA). All recordings were pre-amplified in the DC mode ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
-
bioRxiv - Neuroscience 2021Quote: Eight- to 10-week-old mice were anesthetized with IP ketamine/Xylazine (100mg/kg; 10mg/kg) and placed in a mouse stereotaxic frame (World Precision Instruments, 502600) over a warm heating pad ...
-
bioRxiv - Neuroscience 2020Quote: ... and 2 μl of the mixed viruses (1.0-1.3 × 1013 particles/μl) were injected into each side of the mouse striatum using a Hamilton syringe and a syringe infusion pump (World Precision Instruments, Inc., Sarasota, Florida, USA). Small holes were drilled in the skull ...