Labshake search
Citations for World Precision Instruments :
201 - 250 of 383 citations for 7 4 Aminobenzamido 4 hydroxynaphthalene 2 sulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes were pulled to resistances of ∼3 MΩ from thin-walled glass capillaries (World Precision Instruments, Cat# 1B120F-4). Importantly ...
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were obtained using patch pipettes pulled from borosilicate glass capillaries (1B120F-4, World Precision Instruments). Pipette tip resistances were ∼4-10 MΩ for amacrine cell recordings in the ganglion cell layer ...
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bioRxiv - Cell Biology 2023Quote: TEER was measured at 7-day intervals from 7-28 days in ALI cultures using an Epithelial Tissue Voltohmmeter (EVOM2) and STX2 electrodes (World Precision Instruments, Sarasota, USA). The STX2 electrodes were equilibrated in D-PBS prior to measurement ...
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bioRxiv - Neuroscience 2020Quote: ... Viral construct were administered in 4 injections within GPe using a UMP3 microsyringe pump injector (World Precision Instruments, Sarasota, FL, USA) with 100µl Hamilton syringe ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a resistance of 4–6 MΩ were pulled from borosilicate glass capillaries (1.5 mm outer diameter, 0.86 mm internal diameter, World Precision Instruments, USA) using a micropipette puller (PC-10 ...
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bioRxiv - Neuroscience 2019Quote: ... Recording patch pipettes were pulled with a PC-10 vertical electrode puller (Narishige) from 1.5 mm outer and 1 mm inner diameter patch clamp glass capillaries without filament (WPI, #PG52151-4). Pipette resistance with Ca2+ current recording solutions was ∼3.5 MΩ for pupal and adult MN5 ...
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bioRxiv - Neuroscience 2019Quote: Single-unit recordings in 3 awake Pcp2-Cre-Ai27D mice were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1- to 2-μm tips and 3 to 12 MΩ impedance ...
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bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
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bioRxiv - Neuroscience 2022Quote: Single-unit recordings of Purkinje neurons and cerebellar nuclei neurons were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1-to 2-μm tips ...
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 0.5 and 4 MΩ using a MF-200 microforge (World Precision Instruments). 90% series resistance (Rs ...
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bioRxiv - Neuroscience 2024Quote: ... dorsoventral from the brain surface [DV] 1.9 mm) at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For the optogenetic silencing experiments (Figure 1H–N) ...
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bioRxiv - Neuroscience 2020Quote: Somatic whole cell patch clamp recordings of visually identified CA1 principal cells were performed after 16-23 DIV with microelectrodes (3-8 MΩ) prepared from borosilicate glass capillaries (1B150F-4 World Precision Instruments) using a Sutter P-1000 puller ...
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bioRxiv - Cancer Biology 2019Quote: Melanoma cells were injected into the circulation of 2dpf larvae with a microinjection apparatus and needles were pulled from capillaries (TW100-4, World precision instruments). Transplanted larvae were kept overnight at 28ºC in embryo media ...
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bioRxiv - Neuroscience 2020Quote: ... The administration speed was held constant at 800 nL/min by an injection pump (World Precision Instruments, Inc., UMP 3-4, USA), and the time interval between each injection path was 5 min (43) ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2019Quote: ... TdTomato-expressing (Vglut3lineage) dissociated DRG neurons and HEK cells were performed with patch pipettes pulled from standard borosilicate glass (1B150F-4, World Precision Instruments) with a P-97 puller (Sutter Instruments) ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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bioRxiv - Molecular Biology 2024Quote: ... PRV-CAG-EGFP (BrainVTA, Cat. P03001) was injected into unilateral inguinal WAT (4 × 200 nL) using a 36-gauge beveled needle (WPI, Cat. NF36BV). After the injection ...
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bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Cell Biology 2021Quote: ... and grown with daily media changes until the transepithelial resistance reached 400 ohm/cm2 (4–5 days) measured with an EVOM electrometer (World Precision Instruments, Sarasota, FL).
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Genetics 2019Quote: ... repair templates and nuclease-free water were added to the mixtures and loaded into pulled injection needles (1B100F-4, World Precision Instruments, Sarasota, FL). Individual injected P0 animals were transferred to new 6 cm NGM plates approximately 18 hours after injections ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2021Quote: ... Extracellular recordings were obtained using tungsten electrodes (TM31C40KT, 4-MΩ impedance at 1 kHz or TM31A50KT, 5-MΩ impedance at 1 kHz; World Precision Instruments Inc., Sarasota FL, USA). All recordings were pre-amplified in the DC mode ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
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bioRxiv - Neuroscience 2023Quote: Patch electrodes of 5-7 MΩ resistance (thin wall, filament, 1.5 mm, WPI, Florida, USA) were pulled on DMZ Zeitz-Puller (Zeitz-Instruments Vertriebs GmbH ...
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bioRxiv - Microbiology 2021Quote: ... and 7 days post seeding monolayers were analyzed for TEER (EVOM2; STX3; World Precision Instruments, Inc.). Confluent Transwell cultures were infected with POWV-LI9 (MOI ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were patched using fire-polished 3–7 MΩ resistant borosilicate pipettes (World Precision Instruments, USA) and membrane currents and voltages were recorded in the whole-cell conFIGuration by a Digidata 1440A and a MultiClamp 700A amplifier ...
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bioRxiv - Microbiology 2019Quote: ... monolayers of Huh-7 and C6/36 were cells grown in Fluorodish Cell Cultures (WPI, USA) and the SRB-1 coimmunolabelled with wheat germ agglutinin conjugated with Alexa Fluor488 (Thermofisher ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (7-10 MOhm) were pulled from standard wall glass of 1.5 mm OD (World Precision Instruments). The composition of the bath solution (ACSF ...
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bioRxiv - Neuroscience 2021Quote: NO efflux from cerebellar slices was monitored using a NO-selective amperometric microprobe with a 7 μm tip (WPI), similar to previous work (37) ...
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bioRxiv - Neuroscience 2020Quote: ... Recording patch pipettes (3-7 MΩ) were fabricated from standard-wall borosilicate glass capillary with filament (World Precision Instruments). Intracellular solution for DA PSC recording was (in mM) ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Bioengineering 2023Quote: ... rd10 mice (n=7 per condition) were injected subretinally in the right eye by using an automatic pump injector (WPI). In WT mice (C57BL/6 ...