Labshake search
Citations for World Precision Instruments :
401 - 450 of 484 citations for 3 4 Fluorophenyl 5 fluorobenzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subject to nano-electrospray ionization (nESI) in positive ion mode (at ∼2 kV) with a pulled nanospray tip (World Precision Instruments 1B100-3) prepared as detailed in (48) ...
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bioRxiv - Bioengineering 2021Quote: ... New Jersey) and custom 3D-printed components was assembled around the animal to support a 3-axis micromanipulator (502600, World Precision Instruments, Sarasota, FL) and linear actuator (M-235.5DD ...
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bioRxiv - Neuroscience 2023Quote: ... A circular piece of the skull (3 mm in diameter) centered over the injection was carefully cut out using a sharp drill (Ideal micro drill, World Precision Instruments, Berlin, Germany). The dura was removed with forceps and mild vacuum suction was used to slowly remove the cortex within the craniotomy ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... The ion-selective microelectrodes used to measure hemolymph [K+] were fashioned from glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA) and pulled to a ∼ 1-3 mm tip on a Flaming-Brown micropipette puller (P-1000 ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized with isoflurane (induction, 5%; maintenance, 0.5 to 1.5%) and placed into a stereotaxic frame (World Precision Instruments). The body temperature was maintained at 36° C using a heating pad ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Biophysics 2020Quote: ... After punching a fluid port through the 5 mm thick Sylgard using 0.75 mm diameter biopsy punches (World Precision Instruments) the PDMS chip was bonded onto glass substrates using an 0.3 mbar ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were anesthetized under hypothermia and 5 pulses of 69 nL were injected into the V1 with a nanoliter injector (WPI). Four days later ...
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bioRxiv - Neuroscience 2021Quote: ... Patch electrodes (2–5 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments ...
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bioRxiv - Biophysics 2021Quote: ... The tip of the pipette was cut to an opening diameter between 1~ 5 μm and bent to ~40° using a microforge (DMF1000, WPI).
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... square craniotomy (1-1-5 mm diameter) was made and covered with a coverslip (World Precision Instruments, coverslip No. 1) fitting the size of the bone removed ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly inserted into the brain and 0.5 to 1 µl of viral particles were injected into the hippocampus using a microinjection pump (50 nl/min, WPI). The injection needle was left in place for about 3 minutes and then slowly retracted ...
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bioRxiv - Bioengineering 2024Quote: ... The skin incision was closed using absorbable (5-0 PDS) subcuticular sutures and topical tissue adhesive (GLUture, World Precision Instruments) was applied to the incision ...
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Local field-potential (LFP) activity was recorded using a patch pipette prepared from thin-borosilicate glass (1.5mmOD, 0.84mmID, World Precision Instruments, cat.no. 1B150F-4) pooled and fire-polished on a Flaming/Brown micropipette puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2024Quote: Glass capillary tubes with an inner diameter about 0.2 mm were prepared from thin wall glass capillaries (TW100F-4, World Precision Instruments, Sarasota, FL), using puller (PC-100 ...
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bioRxiv - Neuroscience 2020Quote: ... were applied with a glass pipette (2-3 μm diameter) positioned 20 to 30 μm away from the soma and connected to a picospritzer (World Precision Instruments, adjusted to ~2 psi). All electrophysiological recordings were extracted using Clampfit (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Bioengineering 2021Quote: ... Electrochemical characterizations were performed in a three-electrode configuration, using a thick (2-mm diameter, ~5 mm2) Pt wire (WPI, 99.99%) as counter electrode (CE ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy was sealed with agarose (1.5–2%, dissolved in artificial cerebrospinal fluid) and a 5 mm glass coverslip (World Precision Instruments) was placed over it ...
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...