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Citations for World Precision Instruments :
201 - 250 of 259 citations for R 3 Amino 5 hexynoic acid hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Developmental Biology 2023Quote: ... Ventral longitudinal muscle 6 in abdominal segments 3 or 4 was impaled with two intracellular electrodes (1 mm outer diameter borosilicate capillaries; World Precision Instruments, 1B100F-4) of ∼15 MΩ resistance (3M KCl) ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2021Quote: ... mice were anesthetized with isoflurane (induction, 5%; maintenance, 0.5 to 1.5%) and placed into a stereotaxic frame (World Precision Instruments). The body temperature was maintained at 36° C using a heating pad ...
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bioRxiv - Neuroscience 2020Quote: ... Viral injections were performed using a 32-gauge syringe (Hamilton Syringes Neuros, #65458) at 5-7 nl/min rate using a microsyringe pump (micro4, WPI). For multiple injections ...
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bioRxiv - Biophysics 2020Quote: ... After punching a fluid port through the 5 mm thick Sylgard using 0.75 mm diameter biopsy punches (World Precision Instruments) the PDMS chip was bonded onto glass substrates using an 0.3 mbar ...
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bioRxiv - Neuroscience 2022Quote: ... Pups were anesthetized under hypothermia and 5 pulses of 69 nL were injected into the V1 with a nanoliter injector (WPI). Four days later ...
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bioRxiv - Neuroscience 2021Quote: ... Patch electrodes (2–5 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments ...
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bioRxiv - Biophysics 2021Quote: ... The tip of the pipette was cut to an opening diameter between 1~ 5 μm and bent to ~40° using a microforge (DMF1000, WPI).
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bioRxiv - Neuroscience 2023Quote: ... Cells expressing YFP and with a soma diameter <25 µm were targeted for whole cell recording using pipettes (∼5 MΩ resistance) pulled from borosilicate glass (WPI).
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... square craniotomy (1-1-5 mm diameter) was made and covered with a coverslip (World Precision Instruments, coverslip No. 1) fitting the size of the bone removed ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly inserted into the brain and 0.5 to 1 µl of viral particles were injected into the hippocampus using a microinjection pump (50 nl/min, WPI). The injection needle was left in place for about 3 minutes and then slowly retracted ...
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bioRxiv - Bioengineering 2024Quote: ... The skin incision was closed using absorbable (5-0 PDS) subcuticular sutures and topical tissue adhesive (GLUture, World Precision Instruments) was applied to the incision ...
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Neuroscience 2020Quote: ... were applied with a glass pipette (2-3 μm diameter) positioned 20 to 30 μm away from the soma and connected to a picospritzer (World Precision Instruments, adjusted to ~2 psi). All electrophysiological recordings were extracted using Clampfit (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Bioengineering 2021Quote: ... Electrochemical characterizations were performed in a three-electrode configuration, using a thick (2-mm diameter, ~5 mm2) Pt wire (WPI, 99.99%) as counter electrode (CE ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy was sealed with agarose (1.5–2%, dissolved in artificial cerebrospinal fluid) and a 5 mm glass coverslip (World Precision Instruments) was placed over it ...
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Neuroscience 2024Quote: ... Recordings were obtained using low-resistance pipettes (2.2 - 5 MΩ) pulled from filamented borosilicate glass (World Precision Instruments, Cat #1B150F-4) with a flaming/brown micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated onto either 13 mm 1.5 glass coverslips in 4-well plates or FluoroDish 35 mm dishes ready coated with poly-D-lysine (PDL) (World Precision Instruments). Glass coverslips were coated with 0.1 ug/ul PDL for 20 minutes and washed once with F12 ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...