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Citations for World Precision Instruments :
201 - 250 of 250 citations for Dengue Virus Serotype 3 DIII envelope protein since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with 3% isoflurane (Aerrane, Henry Schein) for 2 min and then transferred to the stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% and monitored the same way as described above for IHKA injection ...
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bioRxiv - Neuroscience 2020Quote: Somatic whole cell patch clamp recordings of visually identified CA1 principal cells were performed after 16-23 DIV with microelectrodes (3-8 MΩ) prepared from borosilicate glass capillaries (1B150F-4 World Precision Instruments) using a Sutter P-1000 puller ...
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bioRxiv - Neuroscience 2020Quote: ... The administration speed was held constant at 800 nL/min by an injection pump (World Precision Instruments, Inc., UMP 3-4, USA), and the time interval between each injection path was 5 min (43) ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: ... to the bregma and 3 mm deep at a flow of 0.2 μL/min using a 10-μL precision microsyringe (World Precision Instruments, Inc) with a 34G needle.
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bioRxiv - Cell Biology 2021Quote: ... were resuspended in 2–3 mg/ml rat-tail collagen I in RM+ medium and seeded on 23-mm FluoroDish cell culture dishes (World Precision Instruments). The collagen with embedded cells was allowed to set at 37°C until collagen contraction was observed (∼30 min) ...
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bioRxiv - Neuroscience 2020Quote: ... and temperature measurements with a digital thermometer (Fisher Traceable Type-K thermometer) with rodent rectal temperature probe (World Precision Instruments, RET-3). For experiments performed in a cold environment ...
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bioRxiv - Cell Biology 2022Quote: Pre-warmed assay media (200 μl) was added to the apical surface of the cultures and TEER was measured using an EVOM-3 meter (World Precision Instruments).
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bioRxiv - Pathology 2022Quote: ... The core body temperature was measured rectally with a Thermometer equipped with a RET-3 mouse rectal temperature probe (WPI, Sarasota, FL).
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bioRxiv - Neuroscience 2020Quote: Electrical stimulation was applied through ACSF filled glass pipette (tip diameter 3–5 µm) connected to a linear stimulus isolator (A395, World Precision Instruments) was used to deliver monopolar electrical stimulation (10–90 µA ...
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bioRxiv - Neuroscience 2020Quote: ... The reference and recording glass electrodes (thin-wall glass capillaries; OD, 1.0 mm; length, 76 mm; World Precision Instruments, cat. # TW100F-3) were pulled using a micropipette puller (Sutter Instrument ...
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bioRxiv - Bioengineering 2020Quote: ... was injected to separate microfluidic channels in the fiber through tubing connected to a standard precision injection apparatus (100 nl/s, NanoFil Syringe and UMP-3 Syringe pump, World Precision Instruments) while the fiber probe was embedded in 0.6% agarose gel (see details of tubing connection in Multifunctional Neuro Probes Assembly).
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bioRxiv - Neuroscience 2020Quote: ... Field stimulations (10 Hz*60 s and 30 Hz*3 s) were performed with an A385 current isolator and a SYS-A310 accupulser signal generator (World Precision Instruments). Glycolysis was inhibited with 2-DG [5 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular field potentials from de CA1 pyramidal neurons were recorded using a borosilicate glass micropipette (1–3 MΩ; RRID:SCR_008593; World Precision Instruments, Sarasota, US) filled with aCSF positioned on the slice surface in the stratum radiatum of CA1 and connected to the headstage of an extracellular recording amplifier (NeuroLog System ...
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bioRxiv - Neuroscience 2023Quote: ... Three circular glass coverslip (2 of 3 mm and one 4mm coverslips) was affixed with a clear silicone elastomer (Kwik-sil, World Precision Instruments). A custom 3D-printed stainless steel headpost was attached on the skull and the rest of exposed skull area and the edge of the cranial window were covered using a dental acrylic (C&B Metabond) ...
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bioRxiv - Neuroscience 2023Quote: ... Animals were injected into their foot with 3-5 µL of 10-5 M ELH using a 10 µL Nanofil syringe (World Precision Instruments) equipped with a 35-gauge needle ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified dsRNA was diluted to 2,000 ng/μL and 3 μg of dsRNA were injected with a Nanoliter (WPI, Sarasota, FL, USA) to the body cavity via the metapleuron under the wing ...
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bioRxiv - Bioengineering 2023Quote: ... DV –4.5 mm) using a microinjection apparatus (10 µL Hamilton syringe #80308, UMP-3 syringe pump, and Micro4 controller; all from World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... A connection cable (Plastics One, model 335–340/3) was used to connect the electrodes to a stimulus isolator (WPI, model A365) and the stimulator (AMPI ...
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bioRxiv - Neuroscience 2023Quote: ... at a rate of 100 nL/minute through a 31-gauge gastight Hamilton syringe attached to a Micro4 Ultra Microsyringe Pump 3 (World Precision Instruments) with a 10 minute waiting period prior to the removal of the needle ...
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bioRxiv - Neuroscience 2024Quote: ... A recording pipette with a resistance of 3–6 MΩ was pulled from a borosilicate glass capillary (World Precision Instruments, Sarasota, FL) using a micropipette puller (Model P-97 ...
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bioRxiv - Neuroscience 2024Quote: ... two circular glass coverslips (one of 3 mm diameter and one of 4 mm) were affixed with a clear silicone elastomer (Kwik-Sil, World Precision Instruments). An extra layer of dental acrylic (C&B Metabond ...
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bioRxiv - Neuroscience 2024Quote: All recordings were performed at 31–33°C with electrodes (3–5 MΏ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with one of two intracellular solutions (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... A negative pressure of 3 PSI was applied to the pipette using a pressure pump (Pneumatic Picopump PV830, World precision instruments, USA). Once the pipette reached the desired depth ...
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bioRxiv - Bioengineering 2022Quote: ... The needle was allowed to sit in place for approximately 5 min and 3-5 μL of viral vector was injected using a microsyringe pump (World Precision Instruments, UMP3) and pump controller (World Precision Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... Viral Injections were unilaterally or bilaterally delivered using a syringe (65458-01, Hamilton, USA) controlled by an ultra-micropump (UMP-3, World Precision Instruments, USA) at a rate of 0.1 µl/min (total volume of 0.6 µl for slice imaging and 0.4 µl for fiber photometry) ...
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bioRxiv - Cancer Biology 2023Quote: ... and the cells were injected at the position 2 mm to the right lateral bregma and 3 mm deep with a flow of 0.2 μL/min utilizing a 10 μL precision micro syringe (World Precision Instruments, Florida, USA) with a 34-gauge needle ...
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bioRxiv - Neuroscience 2023Quote: ... gap-free LFP recordings (15 minute duration) were obtained from the optic tectum using a single-glass microelectrode (WPI glass #TW150 F-3) with approximately 1 μM tip diameter backfilled with 2 mM NaCl internal solution as described previously 29,37–40 ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... We accomplished this by having Varroa feed on pupae in which 3 μl of 1×107 DAPI microspheres in PBS buffer was injected (31 gauge needle, Micro4 microsyringe pump controller (World Precision Instruments, Sarasota, FL). A second group of pupae which served as a control were injected with PBS ...
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Clemizole and Trazodone are Effective Antiseizure Treatments in a Zebrafish Model of STXBP1 DisorderbioRxiv - Neuroscience 2022Quote: ... gap-free local field potential recordings (LFP; 60 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3) filled with 2 mM NaCl internal solution (~1 μm tip diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Whole cell voltage and current-clamp recordings were performed at 32–34 °C with electrodes (3–5MΩ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with intracellular solution of the following composition (in mM) ...
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bioRxiv - Neuroscience 2021Quote: ... gap-free local field potential recordings (LFP; 15 min duration) were obtained from optic tectum using a single glass microelectrode (WPI glass #TW150 F-3); ~ 1 μm tip diameter ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subject to nano-electrospray ionization (nESI) in positive ion mode (at ∼2 kV) with a pulled nanospray tip (World Precision Instruments 1B100-3) prepared as detailed in (48) ...
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bioRxiv - Bioengineering 2021Quote: ... New Jersey) and custom 3D-printed components was assembled around the animal to support a 3-axis micromanipulator (502600, World Precision Instruments, Sarasota, FL) and linear actuator (M-235.5DD ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (3-6 MΩ) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... at least 3 days before the day of injection and the craniotomies were covered with silicone elastomer (Kwik-Cast, World Precision Instruments, FL, USA). Before injection ...
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bioRxiv - Neuroscience 2023Quote: ... A circular piece of the skull (3 mm in diameter) centered over the injection was carefully cut out using a sharp drill (Ideal micro drill, World Precision Instruments, Berlin, Germany). The dura was removed with forceps and mild vacuum suction was used to slowly remove the cortex within the craniotomy ...
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bioRxiv - Developmental Biology 2023Quote: ... Ventral longitudinal muscle 6 in abdominal segments 3 or 4 was impaled with two intracellular electrodes (1 mm outer diameter borosilicate capillaries; World Precision Instruments, 1B100F-4) of ∼15 MΩ resistance (3M KCl) ...
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Biophysics 2022Quote: ... A micropipette was filled with fluorescently labeled protein using a MicroFil flexible needle (MF34G-5, World Precision Instruments, UK). The micropipettes were introduced in each side of the chamber and placed in the middle using micromanipulators ...
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bioRxiv - Biophysics 2021Quote: ... A micropipette was filled with the same buffer as the protein (20 mM Tris and 150 mM NaCl, pH 7.5) using a MICROFIL needle (WPI) and subsequently mounted onto a micromanipulator ...
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bioRxiv - Neuroscience 2020Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2022Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2021Quote: Patch pipettes (3-6 MΩ resistance) were pulled from standard borosilicate glass pipettes (thin-wall borosilicate tubes with filaments, World Precision Instruments, Sarasota, FL, USA) using a vertical puller (PC-10 ...
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bioRxiv - Neuroscience 2020Quote: ... cone tipped 5μl stainless steel injector (SGE Analytical Science, Australia) over 3 min using an infusion pump (UMP3 with SYS4 Micro-controller, World Precision Instruments, Inc., FL, USA). The needle was left in place for 7 min to allow for diffusion of and reduce spread up the infusion tract ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Whole cell current- and voltage-clamp recordings were performed with pipettes (3-7MΩ when filled with intracellular solution) made from borosilicate glass capillaries (World Precision Instruments, Hitchin, Herts, UK) pulled on a P-97 Flaming/Brown micropipette puller (Sutter ...
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bioRxiv - Physiology 2024Quote: ... using glass pulled microelectrodes filled with 3 M KCl (pipette resistance: 30–60 MΩ) connected to a DUO 773 electrometer (World Precision Instruments, Inc., FL, USA). For smooth muscle and endothelium recordings ...
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bioRxiv - Developmental Biology 2020Quote: ... A solution containing 25 ng/µL gRNA and 100 ng/µL Cas9 mRNA or 1000 ng/µL Cas9 protein (Toolgen) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). The insertion and/or deletion (indel ...
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bioRxiv - Developmental Biology 2023Quote: ... A solution containing 25 ng/μL gRNA and 100 ng/μL Cas9 mRNA or 1000 ng/μL Cas9 protein (ToolGen Inc.) was injected into one-cell-stage embryos using a pneumatic microinjector (PV830, WPI). To establish the foxp1b mutant ...
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bioRxiv - Neuroscience 2020Quote: ... were applied with a glass pipette (2-3 μm diameter) positioned 20 to 30 μm away from the soma and connected to a picospritzer (World Precision Instruments, adjusted to ~2 psi). All electrophysiological recordings were extracted using Clampfit (Molecular Devices ...
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bioRxiv - Neuroscience 2023Quote: ... Triturations were carried out using two sequential bore diameters (∼300μm and ∼150μm) of fire-polished glass capillaries (World Precision Instruments; Sarasota, FL, Item No. TW100-3). A total of six triturations were used for female mice and twelve triturations for male mice ...