Labshake search
Citations for World Precision Instruments :
201 - 250 of 412 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Current clamp recordings were performed with pipettes pulled (Sutter P-97) using borosilicate capillary tubes (WPI Cat # 1B150F-4) with open tip resistances of 20 +/- 3 MΩ filled with internal solution containing the following (in mM) ...
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bioRxiv - Cell Biology 2023Quote: ... +2.5 mm L/M and −3.0 mm D/V using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4). Glass micropipettes were mounted to the stereotaxic frame via specialized connectors and attached ...
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bioRxiv - Neuroscience 2024Quote: ... The recording electrodes resistance was typically 4 – 6 MΩ and were pulled from borosilicate glass (World Precision Instruments, UK) using a Sutter P-1000 horizontal puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... of 7 μm in diameter were aspirated into ethanol-cleaned glass capillaries (1.2 mm O.D., 0.68 mm I.D., 4 inches long; World Precision Instruments, FL, USA). The glass capillaries were then pulled using a P-2000 micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2020Quote: ... Patch electrodes (2-4 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments Company ...
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bioRxiv - Neuroscience 2020Quote: ... Patch pipettes were pulled from borosilicate glass capillaries (1.0 mm outside diameter, 0.58 mm inside diameter, 1B100F-4, World Precision Instruments) using a P-1000 puller (Sutter Instruments) ...
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bioRxiv - Neuroscience 2020Quote: Pipettes for OGB injections were pulled from borosilicate glass capillaries (1B150F-4, outer diameter 1.5 mm, inner diameter 0.84 mm, World Precision Instruments) using a micropipette puller (P-97 ...
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bioRxiv - Neuroscience 2020Quote: ... Patch-clamp recording pipettes were fabricated from 1 mm inner diameter borosilicate glass capillaries (World Precision Instruments, item# 1B100F-4). Pipettes were pulled with a multistep horizontal puller (P-1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Borosilicate glass capillaries with a length of 4 inches and an OD of 1.0 mm (World Precision Instruments, FL, USA) were used for microinjection ...
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bioRxiv - Molecular Biology 2022Quote: ... Patch recording pipettes (4-6 MΩ) were pulled from thin-walled borosilicate capillary glass (World Precision Instruments [WPI], Sarasota, FL) on a P-2000 laser electrode puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2022Quote: ... Patch recording pipettes (4-6 MΩ) were pulled from thin-walled borosilicate capillary glass (World Precision Instruments [WPI], Sarasota, FL) on a P-2000 laser electrode puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2023Quote: Whole-cell patch-clamp recordings were obtained using patch pipettes pulled from borosilicate glass capillaries (1B120F-4, World Precision Instruments). Pipette tip resistances were ∼4-10 MΩ for amacrine cell recordings in the ganglion cell layer ...
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bioRxiv - Neuroscience 2023Quote: ... of 7[µm in diameter were aspirated into ethanol-cleaned glass capillaries (1.2[mm O.D., 0.68[mm I.D., 4 inches long; World Precision Instruments, FL, USA). The glass capillaries were then pulled using a P-2000 micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... was used to create a micropipette (tip diameter 30-50 µm) from a micro glass tube (1.0-mm diameter; TW100F-4; WPI, USA). The pulled micropipette was then beveled using a micro grinder (EG-402 ...
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bioRxiv - Neuroscience 2024Quote: ... and Long Pulse Photostimulation (LPP; 4-10 s) waveforms were delivered under the command of a pulse generator (Pulsemaster A300 Generator; WPI) connected to the laser power supply ...
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bioRxiv - Neuroscience 2024Quote: ... 200 nl of the AAV-DIO-EGFP virus (7×10¹² vg/mL) were delivered into the dorsal striatum at a rate of 100 nl min-1 using Micro-4 controller (WPI). In order to ensure the dorsal striatum was targeted ...
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bioRxiv - Neuroscience 2020Quote: ... Viral construct were administered in 4 injections within GPe using a UMP3 microsyringe pump injector (World Precision Instruments, Sarasota, FL, USA) with 100µl Hamilton syringe ...
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bioRxiv - Neuroscience 2022Quote: ... Patch pipettes with a resistance of 4–6 MΩ were pulled from borosilicate glass capillaries (1.5 mm outer diameter, 0.86 mm internal diameter, World Precision Instruments, USA) using a micropipette puller (PC-10 ...
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bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
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bioRxiv - Neuroscience 2022Quote: Single-unit recordings of Purkinje neurons and cerebellar nuclei neurons were performed using borosilicate glass electrodes (1B100F-4, World Precision Instruments) with 1-to 2-μm tips ...
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 0.5 and 4 MΩ using a MF-200 microforge (World Precision Instruments). 90% series resistance (Rs ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 400 nl of AAV was injected at a rate of 80 nl/min and a depth of 0.6 mm into each of 4 injection sites with a microinjector (WPI, Nanoliter 2010) using the following coordinates (AP/ML ...
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bioRxiv - Neuroscience 2024Quote: ... was puffed locally at the surface of the slice near the recorded cell through a micropipette (4-6 MΩ tip resistance; 1 sec puff duration) with a pneumatic drug ejection system [PicoPump PV 820 (WPI, Germany)] in presence or absence of strychnine (10 µM) ...
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bioRxiv - Neuroscience 2024Quote: ... Recordings were obtained using low-resistance pipettes (2.2 - 5 MΩ) pulled from filamented borosilicate glass (World Precision Instruments, Cat #1B150F-4) with a flaming/brown micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2024Quote: ... Muscle cells were subsequently patched using fire-polished borosilicate pipettes with a resistance of 4-6 MΩ (World Precision Instruments, USA). We recorded membrane currents and potentials in a whole-cell configuration using a Digidata 1440A and a MultiClamp 700A amplifier ...
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bioRxiv - Neuroscience 2022Quote: ... glass pipettes (3-8 MΩ, 1B150F-3, World Precision Instruments) were pulled using a horizontal puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2021Quote: ... was assembled prior to injections into the cortex under microscopic control using a microinjection system (UMP3-1, SYS-Micro 4; World Precision Instruments). To increase optogenetic responses after one year ...
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bioRxiv - Cell Biology 2022Quote: ... IVT reticulocyte lysate was backloaded into a needle pulled from a 1 mm glass capillary tube (TW 100F-4, World Precision Instruments) using a P-87 Micropipette Puller (Sutter Instrument) ...
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bioRxiv - Neuroscience 2021Quote: ... 200 nl of 1:1 mixture of AAV5 CAG-FLEx-TCb and AAV8 CAG-FLEx-RG was injected into the PVH at a speed of 50 nl/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). For local circuit mapping (Fig ...
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bioRxiv - Neuroscience 2021Quote: ... Virus was injected at a rate of 40 μl/min controlled via a microsyringe pump (UltraMicroPump3–4; World Precision Instruments, Sarasota, FL). Upon recovery ...
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Prelimbic proBDNF facilitates memory destabilization by regulation of neuronal function in juvenilesbioRxiv - Neuroscience 2022Quote: ... which was arrayed into a 4×8 matrix using 25-μm-diameter tungsten wires (California Fine Wires) in a 35-gauge silica tube (World Precision Instruments). A cannula was attached to a silica tube ...
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bioRxiv - Neuroscience 2022Quote: ... 0.5-inch-long needle attached to a 5 µl Hamilton syringe was mounted to the stereotaxic frame and controlled using a Micro 4 Microsyringe Pump (World Precision Instruments) to infuse 0.5 µl AAV2/PHP.eB-hGfaABC1D-DIO-hTDP43-WT (1.2 × 1013 particles/µl) ...
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bioRxiv - Neuroscience 2022Quote: Pipettes for patch clamping were prepared freshly on the day of electrophysiology experiments from filamented glass capillaries (1B150F-4, World Precision Instruments) using a horizontal pipette puller (P1000 ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
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bioRxiv - Neuroscience 2023Quote: ... A total of 250 nL AAV was dispensed at each cortical layer through a glass-capillary-attached Hamilton syringe at a rate of 40 nL/min using a UltraMicroPump-4 system (World Precision Instruments). To image UDP in hippocampus ...
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV serotype 9 OTp-mCherry was bilaterally injected into the PVH and the supraoptic nucleus at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... 250 nL of either AAV8 hSyn-DIO-hM3Dq-mCherry or AAV5 hSyn-DIO-mCherry was bilaterally injected into the PVH at a speed of 50 nL/min using a UMP3 pump regulated by Micro-4 (World Precision Instruments). The coordinates for injection into the supraoptic nucleus were as follows ...
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Dietary potassium and cold acclimation additively increase cold tolerance in Drosophila melanogasterbioRxiv - Physiology 2024Quote: ... The ion-selective microelectrodes used to measure hemolymph [K+] were fashioned from glass capillaries (TW150-4, World Precision Instruments, Sarasota, FL, USA) and pulled to a ∼ 1-3 mm tip on a Flaming-Brown micropipette puller (P-1000 ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were plated onto either 13 mm 1.5 glass coverslips in 4-well plates or FluoroDish 35 mm dishes ready coated with poly-D-lysine (PDL) (World Precision Instruments). Glass coverslips were coated with 0.1 ug/ul PDL for 20 minutes and washed once with F12 ...
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bioRxiv - Biophysics 2020Quote: ... The signal from the force transducer was amplified using Transbridge TBM4M and digitized using Lab-Trax-4 data acquisition hardware (WPI, Sarasota, FL, USA). Three phases could be distinguished on force curves recorded using LabScribe v3 (iWorks ...
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bioRxiv - Microbiology 2020Quote: The permeability of the differentiated epithelial layer (after 4 weeks of differentiation) was determined by measuring the TEER using an epithelial volt-ohm meter (EVOM2, World Precision Instruments, Inc.). The EVOM2 was calibrated according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: Test compounds were dissolved in aCSF and locally puff-applied with a patch pipette (4–6 MΩ) at low pressure (∼1 psi) controlled by a Pneumatic Picopump (PV821, World Precision Instruments, USA) for 20 to 90s ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was injected via a pulled needle of a borosilicate glass capillary (ID: 0.68mm, OD: 1.2mm; Kwik-fill, World Precision Instruments Inc., # 1B150F-4) that was angled at 50° to the slice using a stereotactic micromanipulator (SM-11 ...
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bioRxiv - Neuroscience 2024Quote: ... Local field-potential (LFP) activity was recorded using a patch pipette prepared from thin-borosilicate glass (1.5mmOD, 0.84mmID, World Precision Instruments, cat.no. 1B150F-4) pooled and fire-polished on a Flaming/Brown micropipette puller (Sutter Instrument ...
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bioRxiv - Molecular Biology 2024Quote: Glass capillary tubes with an inner diameter about 0.2 mm were prepared from thin wall glass capillaries (TW100F-4, World Precision Instruments, Sarasota, FL), using puller (PC-100 ...
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bioRxiv - Cell Biology 2021Quote: ... and grown with daily media changes until the transepithelial resistance reached 400 ohm/cm2 (4–5 days) measured with an EVOM electrometer (World Precision Instruments, Sarasota, FL).
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Cell Biology 2020Quote: ... The resistance between the upper and lower surfaces of IEC monolayers was measured before (Day 4) and after 2 days of stimulation (Day 6) using an EVOM2 epithelial Volt/Ohm meter (World Precision Instruments, FL, USA). The resistance of IEC monolayers was corrected by subtracting the resistance of scaffolds without cells and normalized by multiplying the effective scaffold surface area to provide a trans-epithelial electrical resistance (TEER ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...