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Citations for World Precision Instruments :
151 - 200 of 310 citations for Methyl 2 chloro 3 trifluoromethyl benzoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... Recordings were performed with low resistance (1–3 MΩ) glass pipettes (ID: 0.68 mm, OD: 1.2 mm, WPI, Sarasota, FL) filled with aCSF under current-clamp mode with I=0 configuration ...
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bioRxiv - Neuroscience 2023Quote: ... 30–120 nl of virus were injected at a rate of 3 nl/s at two depths (0.1 mm and 0.2 mm ventral/below pia) using a microsyringe pump (UMP-3, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes were pulled to resistances of ∼3 MΩ from thin-walled glass capillaries (World Precision Instruments, Cat# 1B120F-4). Importantly ...
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bioRxiv - Neuroscience 2022Quote: ... was left in place for 3 and 5 min after the first and second injections (100-nl/min, UMP3 electrical pump, WPI) to prevent backflow of the injected virus solution ...
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bioRxiv - Neuroscience 2024Quote: ... Viral suspensions were loaded in beveled glass capillaries (tip diameter: 15-30 µm) and injected with a micropump (UMP-3, WPI) at a rate of 30-40 nl/min into the parenchyma ...
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bioRxiv - Physiology 2022Quote: ... pulled from glass tubes (1.12 mm inner / 2 mm outer diameter, World Precision Instruments) and bent at 30 ° to allow near-parallel alignment of the fibres with the bottom of the experimental chamber ...
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bioRxiv - Neuroscience 2023Quote: ... Bilateral infusions were achieved using a 35-gauge needle (World Precision Instruments NF35BV-2) and a 10 μL Hamilton Syringe (World Precision Instrument NANOFIL) ...
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bioRxiv - Genetics 2020Quote: ... Glass needles for zebrafish eggs injections were prepared from glass capillary tubes with filament (ø1 mm × 90 mm) (WPI #1B100F-3) using a NARISHIGE dual-stage micropipette puller PC-10 machine ...
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bioRxiv - Biophysics 2020Quote: ... were collected by centrifugation for 3 min at 180 RCF and seeded onto glass bottom dishes (fluorodish; #FD35100, WPI, FL, USA) in CO2-independent medium (#18045-054 ...
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bioRxiv - Neuroscience 2020Quote: ... Germany) and Pulse 8.11 software as described previously.3 Patch pipettes were pulled from borosilicate glass tubing (World Precision Instruments, Germany). The signals were filtered using an eight-pole Bessel filter built into the EPC-9 amplifier and digitized at a frequency ≥ filter cut-off frequency (VR-10B ...
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bioRxiv - Neuroscience 2022Quote: ... The tip of the pipette was cut to an opening diameter of ∼3 μm and bent to ∼40° using a microforge (WPI, DMF1000). Tethers were pulled with a 4 µm diameter polystyrene bead (Spherotech #DIGP-40-2 ...
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bioRxiv - Neuroscience 2022Quote: ... We sealed the craniotomy with silicone gel (Dow DOWSIL 3-4680, sometimes called “artificial dura”) and a silicone sealant (WPI KwikCast). We also implanted a stainless steel pin elsewhere on the dorsal surface of the skull ...
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bioRxiv - Developmental Biology 2024Quote: Erythrocytes were isolated from 3-dpf zebrafish embryos (wild-type and miR-144Δ/Δ) by dissecting their tail using sapphire blade (WPI) and collecting cells in the bleeding buffer (1X PBS containing 2% PBS and 5 mM EDTA) ...
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bioRxiv - Neuroscience 2024Quote: ... Reference glass electrode and recording glass electrode were created by processing Standard Glass Capillaries (Cat # IB150F-3, World Precision Instruments, USA) with glass puller ...
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bioRxiv - Neuroscience 2024Quote: ... We used 3−5 MΩ glass patch pipettes (capillary tubing with 1.5/0.75 mm OD/ID-World Precision Instruments, Sarasota, FL) that were pulled on a P-97 Flaming/Brown type micropipette puller (Sutter Instrument Co. ...
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bioRxiv - Neuroscience 2020Quote: ... driven by a digital Micro2T controller (Model UMP3T-2; World Precision Instruments, Sarasota, FL, USA). After each injection ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Anterior chambers were cannulated with 33-gauge beveled needles (NanoFil, NF33BV-2, World Precision Instruments) attached to micro-manipulators and equilibrated for 30 minutes at 9 mmHg ...
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bioRxiv - Cancer Biology 2023Quote: ... which was experimentally validated using a set Ca2+ calibration buffers (World Precision Instruments, Calbuf-2). Rates of Ca2+ uptake were determined by fitting a single exponential from the initial peak after Ca2+ addition (T = 400 sec ...
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bioRxiv - Neuroscience 2024Quote: ... The filling needle was replaced with a 35-gauge needle (World Precision Instruments, #NF35BV-2), and the syringe was secured to a micromanipulator (Model Kite-L ...
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bioRxiv - Biophysics 2023Quote: ... with resistances of 2-3.5 MΩ (Cat. #TW150F-4, World Precision Instruments, Sarasota, FL, USA). Voltage commands were applied with a MultiClamp 700B amplifier (Axon Instruments ...
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bioRxiv - Microbiology 2023Quote: ... and 14 Trans-epithelial electrical resistance (TEER) was measured (EVOM-2 voltohmmeter, World Precision Instruments) 10 min after medium change ...
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bioRxiv - Neuroscience 2023Quote: Eyes were initially punctured with a beveled needle (NF36BV-2, NanoFil Needles, World Precision Instruments) in the pars plana site that is posterior from the limbus of the eye [41 ...
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bioRxiv - Cell Biology 2024Quote: STX4 EVOM electrodes connected to an EVOM 2 electrical impedance reader (both World Precision Instruments) were used to measure electrical resistance in ohms (Ω) ...
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bioRxiv - Neuroscience 2021Quote: ... was injected into PR at 0.5 µl/min using G33 needle (Hamilton) connected to a 10 µl micro-syringe controlled by a micro-pump (WPI, UltraMicroPump 3). AM cells were recorded both before and 30 min after injection.
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bioRxiv - Neuroscience 2020Quote: Somatic whole cell patch clamp recordings of visually identified CA1 principal cells were performed after 16-23 DIV with microelectrodes (3-8 MΩ) prepared from borosilicate glass capillaries (1B150F-4 World Precision Instruments) using a Sutter P-1000 puller ...
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bioRxiv - Neuroscience 2020Quote: ... The administration speed was held constant at 800 nL/min by an injection pump (World Precision Instruments, Inc., UMP 3-4, USA), and the time interval between each injection path was 5 min (43) ...
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A novel neural stem cell-derived immunocompetent mouse model of glioblastoma for preclinical studiesbioRxiv - Cancer Biology 2020Quote: ... to the bregma and 3 mm deep at a flow of 0.2 μL/min using a 10-μL precision microsyringe (World Precision Instruments, Inc) with a 34G needle.
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bioRxiv - Cell Biology 2021Quote: ... were resuspended in 2–3 mg/ml rat-tail collagen I in RM+ medium and seeded on 23-mm FluoroDish cell culture dishes (World Precision Instruments). The collagen with embedded cells was allowed to set at 37°C until collagen contraction was observed (∼30 min) ...
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bioRxiv - Neuroscience 2020Quote: ... and temperature measurements with a digital thermometer (Fisher Traceable Type-K thermometer) with rodent rectal temperature probe (World Precision Instruments, RET-3). For experiments performed in a cold environment ...
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bioRxiv - Cell Biology 2022Quote: Pre-warmed assay media (200 μl) was added to the apical surface of the cultures and TEER was measured using an EVOM-3 meter (World Precision Instruments).
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bioRxiv - Pathology 2022Quote: ... The core body temperature was measured rectally with a Thermometer equipped with a RET-3 mouse rectal temperature probe (WPI, Sarasota, FL).
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bioRxiv - Neuroscience 2020Quote: Electrical stimulation was applied through ACSF filled glass pipette (tip diameter 3–5 µm) connected to a linear stimulus isolator (A395, World Precision Instruments) was used to deliver monopolar electrical stimulation (10–90 µA ...
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bioRxiv - Neuroscience 2020Quote: ... The reference and recording glass electrodes (thin-wall glass capillaries; OD, 1.0 mm; length, 76 mm; World Precision Instruments, cat. # TW100F-3) were pulled using a micropipette puller (Sutter Instrument ...
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bioRxiv - Bioengineering 2020Quote: ... was injected to separate microfluidic channels in the fiber through tubing connected to a standard precision injection apparatus (100 nl/s, NanoFil Syringe and UMP-3 Syringe pump, World Precision Instruments) while the fiber probe was embedded in 0.6% agarose gel (see details of tubing connection in Multifunctional Neuro Probes Assembly).
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bioRxiv - Neuroscience 2021Quote: ... 1μL of virus was injected in the dorsal hippocampus and 1μl of virus in the ventral hippocampus (either AAV-miR-499 or AAV-Control) over 3 min via a microinjection pump (World Precision Instruments). The infusion cannula was left in place for an additional 3 min thereafter to allow diffusion ...
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bioRxiv - Neuroscience 2020Quote: ... Field stimulations (10 Hz*60 s and 30 Hz*3 s) were performed with an A385 current isolator and a SYS-A310 accupulser signal generator (World Precision Instruments). Glycolysis was inhibited with 2-DG [5 mM ...
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bioRxiv - Neuroscience 2020Quote: ... Extracellular field potentials from de CA1 pyramidal neurons were recorded using a borosilicate glass micropipette (1–3 MΩ; RRID:SCR_008593; World Precision Instruments, Sarasota, US) filled with aCSF positioned on the slice surface in the stratum radiatum of CA1 and connected to the headstage of an extracellular recording amplifier (NeuroLog System ...
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bioRxiv - Neuroscience 2023Quote: ... A connection cable (Plastics One, model 335–340/3) was used to connect the electrodes to a stimulus isolator (WPI, model A365) and the stimulator (AMPI ...
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bioRxiv - Neuroscience 2023Quote: ... at a rate of 100 nL/minute through a 31-gauge gastight Hamilton syringe attached to a Micro4 Ultra Microsyringe Pump 3 (World Precision Instruments) with a 10 minute waiting period prior to the removal of the needle ...
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bioRxiv - Bioengineering 2023Quote: ... DV –4.5 mm) using a microinjection apparatus (10 µL Hamilton syringe #80308, UMP-3 syringe pump, and Micro4 controller; all from World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... Animals were injected into their foot with 3-5 µL of 10-5 M ELH using a 10 µL Nanofil syringe (World Precision Instruments) equipped with a 35-gauge needle ...
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bioRxiv - Molecular Biology 2023Quote: ... The purified dsRNA was diluted to 2,000 ng/μL and 3 μg of dsRNA were injected with a Nanoliter (WPI, Sarasota, FL, USA) to the body cavity via the metapleuron under the wing ...
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bioRxiv - Neuroscience 2024Quote: All recordings were performed at 31–33°C with electrodes (3–5 MΏ) pulled from borosilicate glass (World Precision Instruments, Sarasota, FL) filled with one of two intracellular solutions (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... A negative pressure of 3 PSI was applied to the pipette using a pressure pump (Pneumatic Picopump PV830, World precision instruments, USA). Once the pipette reached the desired depth ...
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bioRxiv - Neuroscience 2024Quote: ... two circular glass coverslips (one of 3 mm diameter and one of 4 mm) were affixed with a clear silicone elastomer (Kwik-Sil, World Precision Instruments). An extra layer of dental acrylic (C&B Metabond ...
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bioRxiv - Neuroscience 2024Quote: ... A recording pipette with a resistance of 3–6 MΩ was pulled from a borosilicate glass capillary (World Precision Instruments, Sarasota, FL) using a micropipette puller (Model P-97 ...
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bioRxiv - Neuroscience 2022Quote: ... Injections were performed with a blunt 33-gauge stainless steel needle (NF33BL-2, World Precision Instruments) and a 10 μL microinjection syringe (Nanofil ...
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bioRxiv - Neuroscience 2023Quote: ... Injections were performed with a blunt 33-gauge stainless steel needle (World Precision Instruments NF33BL-2) and a 10 µL microinjection syringe (World Precision Instruments NanoFil) ...
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bioRxiv - Neuroscience 2024Quote: ... Isoflurane (1-2%; Aerrane, Piramal Enterprises) mixed with oxygen was delivered through a nose cone (WPI). Buprenex (buprenorphine ...
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bioRxiv - Bioengineering 2022Quote: ... The needle was allowed to sit in place for approximately 5 min and 3-5 μL of viral vector was injected using a microsyringe pump (World Precision Instruments, UMP3) and pump controller (World Precision Instruments ...