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Citations for World Precision Instruments :
101 - 150 of 601 citations for 5 2 4 Dimethylphenyl 3 methyl 5 oxovaleric acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... transfected slices were then transferred to the microscope chamber perfused with carbogen (95% O2 / 5% CO2)-bubbled recording aCSF maintained at approximately 30 - 34 °C by an in-line solution heater (WPI). The aCSF contained (in mM) ...
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bioRxiv - Neuroscience 2024Quote: ... AAV viral vectors mixed with FastGreen for visualization (ratio 1:5) were injected using a PicoSpritzer (Cat. num. PV820, WPI) through pulled (P-30 puller ...
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bioRxiv - Neuroscience 2022Quote: ... electrolytic lesions were obtained by passing positive and negative current (20-25 uA) on each electrode contact for 5 s with a stimulus isolator (A365RC, WPI) while the animal was under isofluorane-induced anesthesia ...
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bioRxiv - Neuroscience 2024Quote: ... square craniotomy (1-1-5 mm diameter) was made and covered with a coverslip (World Precision Instruments, coverslip No. 1) fitting the size of the bone removed ...
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bioRxiv - Neuroscience 2024Quote: ... was slowly inserted into the brain and 0.5 to 1 µl of viral particles were injected into the hippocampus using a microinjection pump (50 nl/min, WPI). The injection needle was left in place for about 3 minutes and then slowly retracted ...
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bioRxiv - Bioengineering 2024Quote: ... The skin incision was closed using absorbable (5-0 PDS) subcuticular sutures and topical tissue adhesive (GLUture, World Precision Instruments) was applied to the incision ...
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bioRxiv - Neuroscience 2020Quote: ... Patch electrodes (2-4 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments Company ...
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2022Quote: Mice were anesthetized with 3% isoflurane (Aerrane, Henry Schein) for 2 min and then transferred to the stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% and monitored the same way as described above for IHKA injection ...
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bioRxiv - Neuroscience 2023Quote: ... Three circular glass coverslip (2 of 3 mm and one 4mm coverslips) was affixed with a clear silicone elastomer (Kwik-sil, World Precision Instruments). A custom 3D-printed stainless steel headpost was attached on the skull and the rest of exposed skull area and the edge of the cranial window were covered using a dental acrylic (C&B Metabond) ...
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bioRxiv - Neuroscience 2021Quote: ... Constant current stimuli (0.5 μA-5 mA) were applied using a high-voltage stimulus isolator (A365R, World Precision Instruments, Sarasota, FL). The evoked mono and polysynaptic IPSCs were recorded simultaneously in GCs and SGC pairs ...
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bioRxiv - Systems Biology 2021Quote: ... 1 mM beta-glycerol phosphate and 5 mM of sodium orthovanadate) using a syringe pump (Aladdin AL-1000, World precision instruments). Livers and right kidneys were quickly removed and snap frozen in liquid nitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... was injected iontophoretically (0.8—1.0 μA positive current pulses with a 7 s-on/off cycle for 5 min using A365, World Precision Instruments). The coordinates of the injection were [AP −2.9 ...
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bioRxiv - Bioengineering 2021Quote: ... The culture medium in the trachea lumen and within the chamber was refreshed via a syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and a programmable pump (flow rate ...
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bioRxiv - Bioengineering 2021Quote: ... the culture medium within the inner space of the trachea was replaced with the fresh medium once a day using a programmable syringe pump (flow rate: 5 mL.h-1; volume: 10 mL; AL-4000, World Precision Instruments) and the removed culture medium was collected in a waste reservoir ...
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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The CB1 receptor interacts with cereblon and drives cereblon deficiency-associated memory shortfallsbioRxiv - Neuroscience 2023Quote: Adult mice (2 months-old) were anaesthetized with isoflurane (4%) and placed into a stereotaxic apparatus (World Precision Instruments, Sarasota, FL, US). Adeno-associated viral particles were injected with a Hamilton microsyringe (Sigma-Aldrich #HAM7635-01 ...
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bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were subject to nano-electrospray ionization (nESI) in positive ion mode (at ∼2 kV) with a pulled nanospray tip (World Precision Instruments 1B100-3) prepared as detailed in (48) ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2024Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Physiology 2021Quote: The mixed sample was then transferred to a 5 mL syringe and perfused using an Aladdin AL-1000 syringe pump (World precision instruments, United Kingdom) through the ibidi slide ...
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bioRxiv - Cell Biology 2023Quote: ... The micropipette was bent to a desired degree by heating it over an alcohol lamp and was filled with phosphate-buffered saline (PBS) using the MicroFil (World Precision Instruments, MF34G-5). The micropipette was installed to a micropipette holder ...
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bioRxiv - Cell Biology 2020Quote: ... The resistance between the upper and lower surfaces of IEC monolayers was measured before (Day 4) and after 2 days of stimulation (Day 6) using an EVOM2 epithelial Volt/Ohm meter (World Precision Instruments, FL, USA). The resistance of IEC monolayers was corrected by subtracting the resistance of scaffolds without cells and normalized by multiplying the effective scaffold surface area to provide a trans-epithelial electrical resistance (TEER ...
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bioRxiv - Neuroscience 2022Quote: ... filamented glass capillaries (4”, 1.0 mm outer diameter; World Precision Instruments, 1B100F-4) were pulled using a Sutter P-97 Flaming/Brown type micropipette puller using a custom program with the following parameters ...
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bioRxiv - Neuroscience 2024Quote: ... filamented glass capillaries (4”, 1.0 mm outer diameter; World Precision Instruments, 1B100F-4) were pulled using a Sutter P-97 Flaming/Brown type micropipette puller using a custom program with the following parameters ...
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bioRxiv - Neuroscience 2020Quote: ... pipettes (World Precision Instruments, 1B150F-3) were pulled (Sutter ...
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bioRxiv - Neuroscience 2021Quote: ... M3516)-filled glass pipette (WPI, TW100F-3) and delivered at rate 30 nl/min using a micro-infusion pump (Harvard Apparatus ...
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bioRxiv - Microbiology 2021Quote: ... cRNA was micro-injected into stage 5 or 6 oocytes using a Micro4TM micro-syringe pump controller and A203XVY nanoliter injector (World Precision Instruments, Sarasota, FLA, U.S.A.). Oocytes were maintained in oocyte Ringer’s buffer with added gentamycin (50 μg/ml ...
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bioRxiv - Neuroscience 2024Quote: ... mice were anesthetized and electrolytic lesions were made through individual tetrode leads (5-30μA for 5s; stimulus isolator, World Precision Instruments or A-M Systems). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Recording electrodes (resistance 1-4 MΩ) were pulled from borosilicate glass (WPI, 1B120F-4) with a micropipette puller (Sutter Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... Recording electrodes of 4-6 MΩ were pulled from borosilicate glass (WPI 1B150F-4) with a vertical puller (Narishige P-10) ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... Recording electrodes (resistance 1-4 MΩ) were pulled from borosilicate glass (WPI, 1B120F-4) with a micropipette puller (NARISHIGE ...
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bioRxiv - Neuroscience 2023Quote: ... covered with cells was placed between two plates and an oscillatory pressure-driven fluid flow was propelled through it using 5 mL syringes (Terumo®) mounted on a programmable double syringe pump (AL-4000; World Precision Instruments®, Hertfordshire, UK). The flow rates of 22.45 mL/min and 4.49 mL/min at 0.5 Hz were used to obtain a shear stress magnitude of 0.1 and 0.5 Pa ...
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bioRxiv - Developmental Biology 2020Quote: ... Patch pipettes (1B150F-4, WPI) with a tip resistance of 5–8MΩ were filled with internal solution containing in mM ...
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bioRxiv - Developmental Biology 2022Quote: ... glass capillaries (World Precision Instruments TW100-3) were pulled using a P-97 Flaming Brown needle puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2023Quote: ... Glass capillaries (WPI, cat. No. 1B100-3) coated with a drop of heparin (50U/ml ...
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bioRxiv - Neuroscience 2023Quote: ... Using an UltraMicroPump 3 (World Precision Instruments), 5.5 μg of Rho-BR-EVs were injected into the lateral ventricle at a 1 μl/min rate ...
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bioRxiv - Developmental Biology 2023Quote: ... glass capillaries (World Precision Instruments TW100-3) are pulled using a P-97 Flaming Brown needle puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2021Quote: ... 4-6 MOhm pipettes were prepared from borosilicate glass capillaries (PG10165-4, World Precision Instruments) in a vertical puller (PIP 6 ...