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Citations for World Precision Instruments :
101 - 150 of 537 citations for 4' Bromo 2' fluoro 3 2 methoxyphenyl propiophenone since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... Recording pipettes (2-4MΩ) were pulled from borosilicate glass (Schott no. 8250; World Precision Instruments, Sarasota, FL, USA) with a Sutter P-97 puller (Sutter Instrument ...
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bioRxiv - Neuroscience 2022Quote: ... Embryos were removed and injected one at a time with 2 μL (2μg/μl) DNA containing pAAV-CAG-mRuby2 and CMV-dnKv4.2 using pulled glass micropipettes (WPI) into the lateral ventricle ...
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bioRxiv - Neuroscience 2023Quote: ... were pressure-injected into caudate nucleus (Interaural 9.75 mm) and putamen (Interaural 9.75 mm) by Hamilton syringe mounted into motorized microinjector (UMP3T-2, WPI) held by manipulator (Model 1460 ...
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bioRxiv - Neuroscience 2023Quote: ... AAV virus was then infused via a blunt 36-gauge stainless steel needle (World Precision Instruments NF36BL-2) connected to a microliter injection system (10 µL ...
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bioRxiv - Neuroscience 2024Quote: ... the craniotomies were covered with agarose (2%) and subsequently sealed with silicone elastomer (Kwik-cast, World Precision Instruments). Recordings were performed daily for up to 3 days following the craniotomies.
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bioRxiv - Molecular Biology 2024Quote: ... The electrical resistance of cell monolayers was measured on day 4 (before stimulation) and day 7 (after 3 days of stimulation) using an EVOM2 epithelial Volt/ Ohm meter (World Precision Instruments, FL, USA). The resistance of cell monolayers was corrected by subtracting the resistance of wells without cells and normalized by multiplying by the effective well surface area to provide transepithelial electrical resistance (TEER ...
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bioRxiv - Cell Biology 2020Quote: ... on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100, WPI). 8 hr following transfection ...
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bioRxiv - Immunology 2021Quote: ... Barrier integrity of ALI cultures was monitored by measuring trans-epithelial electrical resistance (TEER, EVOM 2, World Precision Instruments). ALI cultures were validated for use in experiments based on microscopic appearance of appropriate ciliated morphology and TEER > 500 Ω*cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... TEER values were determined daily beginning on day-2 using an epithelial volt/ohm meter (EVOM2, World Precision Instruments) and STX2 series chopstick electrodes as described previously (23).
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bioRxiv - Neuroscience 2024Quote: ... For all surgical procedures mice were anesthetized using 1.5-2% isoflurane and were injected using a Kopf stereotaxic and World Precision Instruments (WPI) microinjection syringe pump with a 10 mL syringe and 33g blunt needle at 50-100 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2024Quote: ... 600nl of vector per hemisphere was injected at 200nl/min into the lateral hypothalamic area (AP/DV/ML = ±1.6/-5.3/ ±0.9 mm) using a microinjection syringe pump (UMP3T-2 World Precision Instruments). For morphological analysis of astrocytes in the LHa viral titres were dropped ten-fold to 1011 GC/ml ...
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bioRxiv - Immunology 2020Quote: 1.5 mm outer diameter and 1.12 mm inner diameter borosilicate glass capillaries were purchased from World Precision Instruments (WPI# TW150-4). We optimized fabrication procedures to obtain micropipettes with 2 μm diameter tips ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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bioRxiv - Neuroscience 2021Quote: ... Patch electrodes (2–5 MΩ) were pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments) on a Sutter P-97 puller (Sutter Instruments ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Neuroscience 2024Quote: ... we applied 1-s pressure pulses (0.4-0.6 psi) every 2 s for 1 min by a microinjector (PV850, World Precision Instruments). In some experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch-clamp recordings were made using electrodes (2-6MΩ) pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments). They were filled with a pipette solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... and injected with a volume of 2 μL at a rate of 20 nL/s (Micro4, World Precision Instruments, USA) into the vitreous cavity (51730D ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...
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bioRxiv - Bioengineering 2021Quote: ... Electrochemical characterizations were performed in a three-electrode configuration, using a thick (2-mm diameter, ~5 mm2) Pt wire (WPI, 99.99%) as counter electrode (CE ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted lateral-side up on a thin layer of 1.5-2% low-melt agarose in a tissue culture dish with a cover-glass bottom (World Precision Instruments) and covered in E3 media ...
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bioRxiv - Neuroscience 2020Quote: ... The craniotomy was sealed with agarose (1.5–2%, dissolved in artificial cerebrospinal fluid) and a 5 mm glass coverslip (World Precision Instruments) was placed over it ...
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bioRxiv - Neuroscience 2020Quote: ... VP neurons were visualized using an Olympus BX51WI microscope and recorded from using glass pipettes (1.3-2 MΩ, World Precision Instruments) filled with internal solution (in mM ...
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... a 2-mm diameter craniotomy was then made over the fiducial mark and covered with KwikCast (World Precision Instruments, FL, USA). The mouse then recovered overnight before the recording session.
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bioRxiv - Cell Biology 2022Quote: ... TEER was measured with was monitored daily using an epithelial tissue volt ohmmeter (EVOMX) with STX-2 chopsticks (World Precision Instruments). TEER measurements were calculated in ohms cm2 after subtracting the blank value for the membrane insert ...
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bioRxiv - Neuroscience 2023Quote: ... 1474) and a 75-200 nL volume of viral vector was injected using a microinjector pump (World Precision Instruments, UMP3T-2) with an attached 10-μL NanoFil syringe (World Precision Instruments ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Neuroscience 2024Quote: ... The craniotomy was covered with agarose (2% in physiological Ringer solution) then sealed with silicon elastomer (Kwik-Cast, World Precision Instruments). This craniotomy was used for acute recording for 2-3 consecutive days (with the probe lowered to a new location each time) ...
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bioRxiv - Neuroscience 2024Quote: ... mounted on a microinjection syringe pump connected to a digital Micro2T controller (Model UMP3T-2; World Precision Instruments, Sarasota, FL, USA). After each injection ...
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bioRxiv - Neuroscience 2024Quote: ... we filled a glass pipettes (2-3 MΩ) with aCSF and delivered monophasic stimulus pulses (2-15 mV, 100 μs/pulse) using a stimulus isolator (A360, WPI, USA). For L2/3 stimulation ...
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bioRxiv - Neuroscience 2020Quote: ... Mice were anesthetized with isoflurane (5% induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2020Quote: ... mice were anesthetized with isoflurane (5 % induction, 1-2 % maintenance; Aerrane, Henry Schein) and secured in a rodent stereotaxic apparatus (Model #502063, World Precision Instruments). Buprenex (Buprenorphine ...
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bioRxiv - Neuroscience 2021Quote: ... was inserted in the dorsal horn to carefully deliver CAV virus at 2 nl/s with the aid of electronically controlled nanoinjector (Nanoliter 2010, WPI, FL, US). Three injections ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 min in a rectangular transparent plexiglass box (induction chamber) and then transferred to a stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% ...
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bioRxiv - Microbiology 2021Quote: ... The dorsal side of their ears was then either exposed to mosquito bites for two minutes or injected with 0.2 μL of sporozoite suspension using a micro syringe (NanoFil 10 μL syringe mounted with a 35G beveled needle, World Precision Instruments), yielding fields of view containing in average 100 parasites ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nl of one of the viral vectors listed above at a titer of 2*1012 particles/ml was injected per site (PMd, amv dlPAG) using a 10 μl nanofil syringe (World Precision Instruments) at 0.08 μl/min ...
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bioRxiv - Genetics 2020Quote: ... phenol red was injected into the yolk of 1- to 2-cell-stage embryos with a Pneumatic PicoPump pv280 (World Precision Instruments). After injection ...
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bioRxiv - Neuroscience 2021Quote: ... Approximately 1 µl (2 x 1010 GC/hemisphere) virus was dispensed using a NanoFil syringe with a 33G beveled needle (World Precision Instruments). The other hemisphere was injected in the same way ...
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bioRxiv - Neuroscience 2023Quote: ... 20 nl of the Cre-dependent helper virus was injected to the HDB (bilateral, n = 2; unilateral, n = 1) using a Nanoliter 2010 microinjection pump (World Precision Instruments). The injection coordinates were defined by a stereotaxic atlas (Paxinos and Franklin ...
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bioRxiv - Cell Biology 2024Quote: ... and injected with 3 μl of AAV9-hSyn-iCre (titer 1 x 1013 to 2 x 1014) into the right lateral ventricle using a nanoliter injector (World Precision Instruments; Micro4 MicroSyringe Pump Controller connected with Nanoliter 2000 ...
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bioRxiv - Neuroscience 2023Quote: ... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings from GPe neurons were obtained using micropipettes (2–4MΩ) made from 1.5 mm OD/1.12 mm ID borosilicate glass with a filament (World Precision Instruments, Sarasota, FL) pulled with a P-97 puller (Sutter Instruments Novato ...
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bioRxiv - Cell Biology 2024Quote: ... Briefly 0.1 to 0.2 x 106 cells were seeded in 2 ml of medium in 6-well dishes or 3cm glass-bottomed microscope dishes (World Precision Instruments) or 0.04 x 106 cells were seeded in 300µl of medium in chambers of an 8-well microscopy slide (Ibidi ...
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bioRxiv - Cell Biology 2024Quote: ... Briefly 0.1 to 0.2 x 106 cells were seeded in 2 ml of medium in 6-well dishes or 3cm glass- bottomed microscope dishes (World Precision Instruments) or 0.04 x 106 cells were seeded in 300µl of medium in chambers of an 8-well microscopy slide (Ibidi ...