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Citations for World Precision Instruments :
651 - 700 of 716 citations for 7 Chloro 1 3 dihydro 5 phenyl 2H 1 4 benzodiazepin 2 thione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Genetics 2019Quote: ... repair templates and nuclease-free water were added to the mixtures and loaded into pulled injection needles (1B100F-4, World Precision Instruments, Sarasota, FL). Individual injected P0 animals were transferred to new 6 cm NGM plates approximately 18 hours after injections ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipette electrodes with a tip resistance between 4 and 6 MΩ were pulled from thin-walled borosilicate glass capillaries (World Precision Instruments, Sarasota, FL) using a P-97 Flaming/Brown Puller (Sutter Instruments ...
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bioRxiv - Cell Biology 2019Quote: ... cells were plated as single cells (5 × 105 cells) on 35 mm FluoroDish Cell Culture Dishes (World Precision Instruments, cat. WD35-100) and allowed to attach for 72 h ...
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bioRxiv - Neuroscience 2019Quote: ... Anesthesia was induced at 5% isofluorane in O2/N2O mixture (Rothacher Medical GmbH., Switzerland) and mice were placed in the stereotaxic frame (World Precision Instruments). During surgery ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Biophysics 2024Quote: ... A Polydimethylsiloxan (PDMS) stencil with a circle area of 5 [mm] was placed inside a bottom glass dish (FluoroDish, World Precision Instruments), which is a 35 [mm] diameter glass bottom dish ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch-clamp recordings were made using electrodes (2-6MΩ) pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments). They were filled with a pipette solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... and injected with a volume of 2 μL at a rate of 20 nL/s (Micro4, World Precision Instruments, USA) into the vitreous cavity (51730D ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2023Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Microbiology 2019Quote: ... and sporozoites were injected intradermally into the ear pinna in a total volume of 0.2 μl using a NanoFil-10 μl syringe with a NF33BV-2 needle (World Precision Instruments). All sporozoite injections were completed within 30 min of salivary gland dissection to ensure that imaging and parasite conditions remained constant ...
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bioRxiv - Microbiology 2019Quote: ... falciparum sporozoites were then injected into the dermis in a total volume of 0.2 μl using a NanoFil-10 μl syringe with a NF33BV-2 needle (World Precision Instruments). The human skin was then moistened with RPMI medium ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted lateral-side up on a thin layer of 1.5-2% low-melt agarose in a tissue culture dish with a cover-glass bottom (World Precision Instruments) and covered in E3 media ...
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bioRxiv - Neuroscience 2020Quote: ... VP neurons were visualized using an Olympus BX51WI microscope and recorded from using glass pipettes (1.3-2 MΩ, World Precision Instruments) filled with internal solution (in mM ...
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bioRxiv - Bioengineering 2022Quote: ... a 2-mm diameter craniotomy was then made over the fiducial mark and covered with KwikCast (World Precision Instruments, FL, USA). The mouse then recovered overnight before the recording session.
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bioRxiv - Cell Biology 2022Quote: ... TEER was measured with was monitored daily using an epithelial tissue volt ohmmeter (EVOMX) with STX-2 chopsticks (World Precision Instruments). TEER measurements were calculated in ohms cm2 after subtracting the blank value for the membrane insert ...
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bioRxiv - Neuroscience 2023Quote: ... 1474) and a 75-200 nL volume of viral vector was injected using a microinjector pump (World Precision Instruments, UMP3T-2) with an attached 10-μL NanoFil syringe (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
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bioRxiv - Neuroscience 2019Quote: Eight to eleven weeks old injected mice were anesthetized with 5% of isoflurane a 1.5% of oxygen into the anesthesia system box (World Precision Instruments, Ref. EZ-B800) for 5 minutes ...
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bioRxiv - Physiology 2021Quote: The mixed sample was then transferred to a 5 mL syringe and perfused using an Aladdin AL-1000 syringe pump (World precision instruments, United Kingdom) through the ibidi slide ...
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bioRxiv - Cell Biology 2023Quote: ... The micropipette was bent to a desired degree by heating it over an alcohol lamp and was filled with phosphate-buffered saline (PBS) using the MicroFil (World Precision Instruments, MF34G-5). The micropipette was installed to a micropipette holder ...
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bioRxiv - Neuroscience 2021Quote: ... was inserted in the dorsal horn to carefully deliver CAV virus at 2 nl/s with the aid of electronically controlled nanoinjector (Nanoliter 2010, WPI, FL, US). Three injections ...
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bioRxiv - Neuroscience 2022Quote: ... for 2 min in a rectangular transparent plexiglass box (induction chamber) and then transferred to a stereotaxic apparatus (Model #502063, World Precision Instruments). Anesthesia was then lowered to 1-2% ...
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bioRxiv - Microbiology 2021Quote: ... The dorsal side of their ears was then either exposed to mosquito bites for two minutes or injected with 0.2 μL of sporozoite suspension using a micro syringe (NanoFil 10 μL syringe mounted with a 35G beveled needle, World Precision Instruments), yielding fields of view containing in average 100 parasites ...
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bioRxiv - Neuroscience 2021Quote: ... 40 nl of one of the viral vectors listed above at a titer of 2*1012 particles/ml was injected per site (PMd, amv dlPAG) using a 10 μl nanofil syringe (World Precision Instruments) at 0.08 μl/min ...
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bioRxiv - Neuroscience 2022Quote: ... Recordings from GPe neurons were obtained using micropipettes (2–4MΩ) made from 1.5 mm OD/1.12 mm ID borosilicate glass with a filament (World Precision Instruments, Sarasota, FL) pulled with a P-97 puller (Sutter Instruments Novato ...
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bioRxiv - Neuroscience 2023Quote: ... The virus solution (0.4-0.6 µL) was injected at a rate of 2 nL/s with a microinjection pump (either Neurostar NanoW or WPI NanoLiter 2010). The pipette was slowly withdrawn at least 10 minutes after the completion of the injection ...
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bioRxiv - Microbiology 2021Quote: ... cRNA was micro-injected into stage 5 or 6 oocytes using a Micro4TM micro-syringe pump controller and A203XVY nanoliter injector (World Precision Instruments, Sarasota, FLA, U.S.A.). Oocytes were maintained in oocyte Ringer’s buffer with added gentamycin (50 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... with a 33-G beveled needle that was mounted into a motorized microinjector (Legato130, KD Scientific or UMP3T-2, WPI, Sarasota, FL, USA). Other surgical procedures and viral injection was as described previously (15).
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bioRxiv - Neuroscience 2020Quote: ... at a rate of 0.2 μL/min with a glass micropipette connected to a Hamilton syringe controlled by stereotaxic syringe holder (SYS-Micro4, WPI, Sarasota, FL, USA). The needle was removed 10 min later ...