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Citations for World Precision Instruments :
551 - 600 of 632 citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Intracortical injections were made at multiple sites (∼5-9 sites per cranial window, spaced 0.5-1 mm apart) using a microinjector (NanoFil, World Precision Instruments) fit with a 36G beveled needle (NF36BV-2 ...
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bioRxiv - Bioengineering 2023Quote: ... Drugs were infused over 5 minutes intravenously via a tail vein catheter with an infusion pump (World Precision Instruments, Sarasota, FL). Focused ultrasound was applied after 2.5 minutes of drug infusion for 2.5 minutes (250 kHz ...
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bioRxiv - Cell Biology 2020Quote: ... on to 1×105 adhered U–2–OS or U–2–OS Flp-In T-Rex cells in 2 mL media in a 3.5 cm glass bottom dish (FD35–100, WPI). 8 hr following transfection ...
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bioRxiv - Immunology 2021Quote: ... Barrier integrity of ALI cultures was monitored by measuring trans-epithelial electrical resistance (TEER, EVOM 2, World Precision Instruments). ALI cultures were validated for use in experiments based on microscopic appearance of appropriate ciliated morphology and TEER > 500 Ω*cm2 ...
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bioRxiv - Cell Biology 2021Quote: ... TEER values were determined daily beginning on day-2 using an epithelial volt/ohm meter (EVOM2, World Precision Instruments) and STX2 series chopstick electrodes as described previously (23).
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bioRxiv - Neuroscience 2024Quote: ... For all surgical procedures mice were anesthetized using 1.5-2% isoflurane and were injected using a Kopf stereotaxic and World Precision Instruments (WPI) microinjection syringe pump with a 10 mL syringe and 33g blunt needle at 50-100 nL/min ...
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bioRxiv - Neuroscience 2024Quote: ... Embryos were loaded into a 2% agarose gel mold and a pre-pulled glass needle (World Precision Instruments, Sarasota) was filled with 10 μL of the injection medium ...
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bioRxiv - Neuroscience 2024Quote: ... 600nl of vector per hemisphere was injected at 200nl/min into the lateral hypothalamic area (AP/DV/ML = ±1.6/-5.3/ ±0.9 mm) using a microinjection syringe pump (UMP3T-2 World Precision Instruments). For morphological analysis of astrocytes in the LHa viral titres were dropped ten-fold to 1011 GC/ml ...
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bioRxiv - Neuroscience 2022Quote: ... A standard micropipette injection protocol was used to make all injections into the brain or spinal cord using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2020Quote: MC3T3-E1 Subclone 4 (ATCC® CRL-2593™) preosteoblastic cells were cultured on duplicate samples of tissue-cultured polystyrene (Fluorodish, World Precision Instruments) and commercially available argon-treated BC (CoArBC) ...
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bioRxiv - Biophysics 2023Quote: ... A standard micropipette injection protocol was used to make all injections into the brain using pulled borosilicate glass micropipettes (WPI, Sarasota, FL, #1B100-4) that were ground to a 35° beveled tip with 150–250 μm inner diameter ...
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bioRxiv - Bioengineering 2024Quote: Molds for the device assembly were fabricated using PDMS and biopsy punches of 4 mm and 7 mm diameters (World Precision Instruments, Sarasota, FL). A large well was made in the PDMS with the 7 mm punch ...
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bioRxiv - Neuroscience 2024Quote: ... A Hamilton syringe (Hamilton 7635-01) was filled with either PBS or C9-ASO at 50 ng/μL and attached to a microinjection pump (World Precision Instruments Micro 4) on a stereotactic arm ...
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bioRxiv - Cell Biology 2021Quote: ... was injected into each hemisphere of the M1 motor cortex using a glass pipette (tip diameter 5∼8 mm) at a rate of 100 nl/min using a syringe pump (Micro4; World Precision Instruments). The injection site was standardized among animals by using stereotaxic coordinates (ML = ±1.50 ...
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bioRxiv - Neuroscience 2022Quote: ... rats were deeply anaesthetised with urethane and lesions were made at the tip of the tetrodes using a 30 μA unipolar current for 5 – 10 s (Stimulus Isolator, World Precision Instruments). Then ...
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bioRxiv - Neuroscience 2022Quote: ... Neural signals were amplified (20,000 X) and filtered (300 Hz – 5 kHz) through an isolated amplifier (ISO-Dam 80, WPI, Sarasota, FL, USA). The microelectrode was manually guided into a cutaneous fascicle by delivering weak electrical pulses (0.2 ms ...
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bioRxiv - Neuroscience 2022Quote: ... They were filled with a 1 M KCl solution using a MicroFil non-metallic syringe needle (MF 34G-5, World Precision Instruments); a few recordings were done with 3 M KCl (no particular change was noticed).
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bioRxiv - Biophysics 2023Quote: ... using a Sutter P-97 puller and heat-polished to final resistances between 1 and 5 MΩ using a MF-200 microforge (World Precision Instruments). An agar bridge (1M KCl ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated for 60 min in 5% CO2 at 37°C in suspension before adding to glass-bottom dishes (FluoroDish, FD35-100, World Precision Instruments) or 12-mm diameter coverslips (Fisherbrand 1254580 ...
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bioRxiv - Neuroscience 2021Quote: ... 2.0-2.3 mm lateral to midline and 2.0-2.5 mm posterior to bregma) at a rate of 60 nL/min at a concentration of 2 mM using a Nanoliter 2020 system (WPI). Optogenetic silencing of V1 was achieved by the use of mice in which channelrhodopsin-2 expression was driven by cre in parvalbumin positive interneurons (Ai32 x PVcre) ...
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bioRxiv - Neuroscience 2023Quote: ... 1010 gc AAV8 in 1-2 μl was stereotactically injected with 15 nl/sec using a microinjection system (Nanoliter 2010, WPI) at (X ...
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bioRxiv - Neuroscience 2024Quote: ... we applied 1-s pressure pulses (0.4-0.6 psi) every 2 s for 1 min by a microinjector (PV850, World Precision Instruments). In some experiments ...
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bioRxiv - Neuroscience 2023Quote: ... Patch pipettes (2–4MU) were pulled from filamental borosilicate glass capillaries (1.5 mm OD, 0.84 mm ID; World Precision Instruments) Patch pipettes were filled with internal solution containing (mM) ...
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Red Algae-Derived Mineral Intervention to Counter Pro-inflammatory Activity in Human Colon OrganoidsbioRxiv - Molecular Biology 2022Quote: ... TEER values were determined daily with an Epithelial Volt ohm meter 2 (EVOM2) epithelial volt/ohm meter (World Precision Instruments) and STX2 series chopstick electrodes.
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bioRxiv - Biochemistry 2023Quote: ... 1×105 U-2 OS or U-2 OS Flp-In T-Rex cells were seeded in 35 mm glass bottom dishes (FD35-100, WPI); cells were transfected with 1-2 µg of pcDNA5 FRT/TO plasmids containing the gene of interest using GeneJuice transfection reagent (Cat#70967 ...
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bioRxiv - Neuroscience 2023Quote: ... The compound solutions were perfused into the chamber and cells via a multichannel perfusion system (MPS-2, World Precision Instruments).
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bioRxiv - Neuroscience 2023Quote: ... and injected with a volume of 2 μL at a rate of 20 nL/s (Micro4, World Precision Instruments, USA) into the vitreous cavity (51730D ...
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bioRxiv - Neuroscience 2023Quote: ... Whole-cell patch-clamp recordings were made using electrodes (2-6MΩ) pulled from borosilicate glass capillaries (1.5 mm OD, 1.12 mm ID; World Precision Instruments). They were filled with a pipette solution containing (in mM) ...
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bioRxiv - Neuroscience 2023Quote: ... and 26-gauge needle (Hamilton) at a flow rate of 100 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2023Quote: ... Recording pipettes (10-15 MΩ) were pulled from borosilicate capillary glass (BF150-86-10, Sutter Instruments, Novato, USA or 1B150F-4, World Precision Instruments, Sarasota, USA) in a horizontal pipette puller (P-1000 Micropipette puller ...
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bioRxiv - Genetics 2023Quote: ... 0.5nl of the mixtures were directly injected into the yolk of freshly layed zygotes using a glass capillary needle [TW120-4) (Kwik-Fil, World Precision Instruments, Inc., Hertfordshire, UK)] with the help of a pedal controlled microinjector (PV820 Pneumatic PicoPump ...
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bioRxiv - Neuroscience 2024Quote: ... plasmid was delivered using a glass pipette at a flow rate of 50 nl/min driven by a microsyringe pump controller (World Precision Instruments, model Micro 4). The syringe needle was left in place for 10 min and was completely withdrawn 17 min after viral delivery ...
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bioRxiv - Neuroscience 2021Quote: ... we filled the central barrel (with the tungsten electrode inside) with superglue using a syringe and fine flexible injection cannula (MicroFil 28 AWG, MF28G67-5, World Precision Instruments, Ltd.). We found that if we did not fill (most of ...
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bioRxiv - Neuroscience 2021Quote: ... Injectors were connected via flexible polyethylene tubing to 5-µl SGE micro-syringes mounted on a microinfusion pump (sp200IZ, World Precision Instruments, UK). A volume of 0.5µl/side of either 0.9 % sterile saline (vehicle ...
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bioRxiv - Neuroscience 2023Quote: ... Electrical hindpaw stimulation was applied with 1 msec 5 mA current pulses using an isolated current stimulator (A382, World Precision Instruments, Sarasota, Florida). For each stimulation condition ...
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bioRxiv - Neuroscience 2022Quote: ... cells were treated with 12-h αSN-AGs (5% v/v) for 16 h and TEER was measured using the EVOM2 apparatus with STX2 chopstick electrodes (World Precision Instruments, USA).
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bioRxiv - Neuroscience 2024Quote: ... Bipolar stimulation electrodes were placed on the unopened spinal cord to transmit low-intensity (5–8 V) electrical pulses generated by an isolated stimulator (A 360, WPI, Sarasota, FL, USA). This allowed antidromic activation of the M-cell axons ...
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bioRxiv - Neuroscience 2021Quote: ... +/−2.6mm ML, −4.5mm DV) using a 10μL syringe (nanofil, World Precision Instrument, USA) with NanoFil Needle (NF33BV-2, World Precision Instruments, USA) at a rate of 100nL/min (Micro4 pump ...
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bioRxiv - Neuroscience 2021Quote: ... then mounted lateral-side up on a thin layer of 1.5-2% low-melt agarose in a tissue culture dish with a cover-glass bottom (World Precision Instruments) and covered in E3 media ...
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bioRxiv - Neuroscience 2020Quote: ... VP neurons were visualized using an Olympus BX51WI microscope and recorded from using glass pipettes (1.3-2 MΩ, World Precision Instruments) filled with internal solution (in mM ...
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bioRxiv - Bioengineering 2022Quote: ... a 2-mm diameter craniotomy was then made over the fiducial mark and covered with KwikCast (World Precision Instruments, FL, USA). The mouse then recovered overnight before the recording session.
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bioRxiv - Cell Biology 2022Quote: ... TEER was measured with was monitored daily using an epithelial tissue volt ohmmeter (EVOMX) with STX-2 chopsticks (World Precision Instruments). TEER measurements were calculated in ohms cm2 after subtracting the blank value for the membrane insert ...
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bioRxiv - Neuroscience 2023Quote: ... 1474) and a 75-200 nL volume of viral vector was injected using a microinjector pump (World Precision Instruments, UMP3T-2) with an attached 10-μL NanoFil syringe (World Precision Instruments ...
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bioRxiv - Cell Biology 2023Quote: ... was injected using a 5-µl Hamilton syringe at a rate of 0.25µl/min during 2 min with a mini-pump (UltraMicroPump III, World Precision Instruments, USA). The same volume of the dye Evans Blue (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... A suspension of 100,000 cells/2 µl/injection was then slowly injected using an UltraMicroPump III and a Micro4 Controller (World Precision Instruments). Post-surgical pain was controlled with SQ carprofen ...
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bioRxiv - Neuroscience 2024Quote: ... mounted on a microinjection syringe pump connected to a digital Micro2T controller (Model UMP3T-2; World Precision Instruments, Sarasota, FL, USA). After each injection ...
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bioRxiv - Neuroscience 2024Quote: ... The craniotomy was covered with agarose (2% in physiological Ringer solution) then sealed with silicon elastomer (Kwik-Cast, World Precision Instruments). This craniotomy was used for acute recording for 2-3 consecutive days (with the probe lowered to a new location each time) ...
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bioRxiv - Neuroscience 2024Quote: ... we filled a glass pipettes (2-3 MΩ) with aCSF and delivered monophasic stimulus pulses (2-15 mV, 100 μs/pulse) using a stimulus isolator (A360, WPI, USA). For L2/3 stimulation ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... SM preparations were fixed between a stationary stainless steel hook and an isometric force transducer (AE 801, SensoNor A/S, Norten, Norway) coupled to an AD converter Lab-Trax 4/16 (World Precision Instruments, Inc., Sarasota, FL, USA). The data were continuously recorded using DataTrax2 (World Precision Instruments ...
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bioRxiv - Physiology 2021Quote: The mixed sample was then transferred to a 5 mL syringe and perfused using an Aladdin AL-1000 syringe pump (World precision instruments, United Kingdom) through the ibidi slide ...